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Citations for Polysciences :
2101 - 2150 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 75 µl of a 5000x diluted stock solution of polystyrene beads (Polysciences, USA) in ethanol (Carl Roth ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transiently transfected with the expression plasmids using polyethylenimines (PEI, Polysciences). In details ...
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bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg polyethylenimines (PEIs, Polysciences) in 50 mL SMM 293T-II medium (Sino Biological Inc. ...
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bioRxiv - Biophysics 2021Quote: ... Cells in a single dish were transfected with 1.25 μg of c-term EGFP tagged hTMEM175 plasmid using 3.75 μg of PEI 25 k (Polysciences, Inc). Electrophysiological recordings were performed 48-72 hr after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Fixed neurons were imaged on a confocal laser-scanning microscope Leica TCS SP8 with identical settings used for all samples ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... sections were mounted (Aqua-Poly/Mount, Polysciences), and the images acquired with a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... followed by 1360µL PEI (Polyethylenimine, Polysciences, 24765-2). 5 mL of DNA-transfection mixture was added to each T150 flask after a 15 minute incubation.
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bioRxiv - Neuroscience 2021Quote: ... and slides were mounted with Aqua-Poly-mount (Polysciences) or cell plate wells were covered with glycerol (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: Cell transfection was performed with lineal polyethylenimine (MW 25 kDa; Polysciences, Warrington, PA, USA). Parenteral HEK-293T cells (8×106 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were covered with Aqua-Poly/Mount (Polysciences). A 63X oil objective (NA = 1.4 ...
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bioRxiv - Neuroscience 2021Quote: ... 1.5 × 107 cells were plated the day before the PEI-mediated transfection (Polysciences). For each plate were used 30μg of pAdDeltaF6 helper plasmid (Addgene plasmid # 112867) ...
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bioRxiv - Neuroscience 2021Quote: ... mixed with the transfection reagent polyethylenimine hydrochloride (PEI Max, 24765-1, Polysciences, Inc., Warrington, PA, USA) at a 1:2 ratio (W/V) ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were stained with 1 mL of 2% uranyl acetate (Polysciences #21447-25) in water for 30 min at room temperature and then briefly washed with 50% ethanol (Pharmco #111000200 ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips were mounted with Aqua-Poly/Mount (Polysciences) and dried overnight in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 67.5 µg of polyethylenimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 µg of plasmid encoding a spike variant ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 100 µg plasmid in complex with 250 µg PEI MAX 4000 (Polysciences, Inc, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... mixed with the transfection reagent polyethylenimine (PEI) hydrochloride (PEI Max, 24765-1, Polysciences, Inc., Warrington, PA, USA) at a 1:2 ratio (W/V) ...
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bioRxiv - Molecular Biology 2021Quote: ... and polyethylenimine (Polysciences, # 23966). Plasmids and polyethylenimine were diluted in Opti-MEM™ ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were incubated for 3 h at RT in the mixture of EtOH and LR White resin (Polysciences, Inc., Warrington, PA, USA) in the following proportions ...
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bioRxiv - Biochemistry 2021Quote: ... and after 4 h the cells were transfected using 60 μg polyethyleimine (PEI, Polysciences, Warrington, PA, USA). Plasmid DNA in an amount of 1.5 μg was diluted in buffer containing 0.15 M NaCl and 20 mM HEPES (pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... 2500 sterilized FITC-tagged microspheres (Polysciences Inc., PA, USA) suspended in a 100µl of PBS were slowly injected into the CCA using a 33G hypodermic needle (TSK Laboratory International ...
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bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Neuroscience 2021Quote: ... sections were coverslipped with Aqua-Poly/Mount (Polysciences, Inc). Images were obtained with an Axio Scan.Z1 digital slide scanner (Zeiss ...
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bioRxiv - Cancer Biology 2021Quote: High titer lentivirus was generated by transfecting HEK293FT cells with a mixture containing three plasmids and 25-kDa linear polyethyleneimine (PEI) (Polysciences, Inc. PA, USA, 239662). In brief ...
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bioRxiv - Cell Biology 2021Quote: ... the pigmentation of RPE cells was removed using Melanin Bleach Kit (Polysciences), followed by 3 washes with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly sub-confluent HEK 293 cells were transfected with branched or linear PEI (Polysciences, Inc.) 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and combined with 1μg/μL polyethylenimine (PEI; Polysciences Inc.) in a 3:1 ratio of PEI:DNA concentration ...
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bioRxiv - Cell Biology 2021Quote: ... Six□micrograms of PEI-Max (Polysciences, #24765-1) were used for transfection ...
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bioRxiv - Biophysics 2021Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Cell Biology 2021Quote: ... linear (PEI, MW-25,000; Polysciences Inc.) or Lipofectamine 2000 (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Streptavidin-conjugated Yellow-Green latex beads (2 μm diameter, 5 x 108 beads/preparation, Polysciences) were washed with 1% BSA in PBS and incubated with Biotin-SP (long spacer)-conjugated Fab fragments of goat-anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: Latex NH2-beads (3 μm diameter, 3.5 x 108 beads/preparation, Polysciences) were activated with 8% glutaraldehyde in 0.5 ml PBS for 120 min under rotation at room temperature ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells grown in 60 mm dish to 70-80 % density were transfected with a mixture of 4 μg plasmid DNA and 20 μg polyethylenimine (Polysciences). Cell lysates were extracted 24 hrs after transfection.
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg/mL of each expression vector using 2 μg/mL Polyethyleneimine “MAX” (PolySciences) (Hirayama et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Transient transfections were performed using 25 kDa linear polyethylenimine (Polysciences) (80 ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 µL of fluorescent microspheres (0.2 µm, Polysciences Fluoresbrite® YO Carboxylate Microspheres catalog number 19391 ...
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bioRxiv - Cell Biology 2021Quote: ... Collagens in the organoids were stained using Masson’s Trichrome Stain Kit (Polysciences) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Transfection of Capsaspora cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) as described previously (19 ...
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bioRxiv - Cell Biology 2021Quote: ... The Huh7 cells were transfected with indicated plasmid using Polyethyleneimine HCl MAX (Polysciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using linear polyethylenimine (Polysciences Inc.) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were mounted in Aqua-Poly/Mount (Polysciences, Inc.) prior to imaging.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Treated rat explants were fixed in 4% PFA (Polysciences, Inc., Warrington, PA, USA) at room temperature for 40 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were first fixed using 4% formaldehyde (pH 7.4) (Polysciences, Inc.) for 10 minutes at room temperature ...