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Citations for VWR :
1451 - 1500 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Equal spotting was controlled by simultaneously spotting cells using a multi-channel ultra-high-performance pipette (VWR International). Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD) ...
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bioRxiv - Immunology 2019Quote: ... or with 5×104 B16F10 wild type (WT) or B16F10-Globin-SL8-intron cells along with matrigel (VWR). Five days after challenge ...
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bioRxiv - Neuroscience 2021Quote: ... The cortex was dissected and homogenized into a single cell suspension using a tissue homogenizer (5 cm3, VWR) and filtered m strainer (Falcon) ...
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bioRxiv - Cell Biology 2021Quote: An early passage iMPC clone was trypsinized and filtered using a 40 μm cell strainer (VWR, 734-0002) to filter out debris and fragments of myofibers ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK293T and HEK293TERK2-EGFP cells were cultured in DMEM media supplemented with high glucose and L-glutamine (VWR) supplemented with 10% Hyclone FetalClone I serum (VWR ...
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bioRxiv - Microbiology 2020Quote: ... The flow cell was pre-incubated with microscopy medium and a bottom coverslip (25 mm No. 1.5; VWR) was attached to the cell ...
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bioRxiv - Cancer Biology 2020Quote: Cell line MDA MB 436 was propagated in DMEM media supplemented with 10% FBS (VWR Seradigm Life Science), 1% L-glutamine and 1% sodium pyruvate (Life Technology-Thermo-Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were then washed 1x with PBS and resuspended in RIPA lysis buffer (VWR, cat # 97063-270) supplemented with protease (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... purified cells were diluted and imaged at 100x magnification within a 20 μm counting chamber (VWR 15170-048). Images were analyzed using FIJI/ImageJ 1.52p (Java 1.8.0_172 ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 104 target cells were pipetted per well into 96-well black plates with clear-bottom (VWR/Corning) and kept in the dark for 20 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... after which the media was changed to complete growth medium and the cells expanded in T182.5 flasks (VWR) in monolayer and until confluent (between 10-14 days) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK T-REx cells were transfected with pcDNA4/TO/hTRPC3 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... For TPE-FLIM imaging the cells were seeded on 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ at room temperature.
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then washed with PBS thrice and treated with 1.6 mM sodium metaperiodate (VWR, 13798-22) for 20 minutes at 4C for oxidation of the N-linked sugar residues ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated off and the cells were resuspended in 500 μl RiboZol RNA extraction reagent (VWR). Tubes were then vortexed and 100 μl chloroform was added to the tube ...
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bioRxiv - Plant Biology 2022Quote: ... The latter was captured in a 300 mL beaker and poured through a cell strainer (VWR, Radnor, USA) with a nominal pore size of 100 µm ...
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bioRxiv - Microbiology 2022Quote: ... cells from a rocking culture of swimmers were added to 60 mm non-treated tissue culture plates (VWR) in 2 mL of BHI ...
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bioRxiv - Neuroscience 2023Quote: ... The solution containing dissociated neurons was passed through a 100 µm sterile cell strainer (VWR, Cat# 21008-950) into a 50 mL conical tube ...
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bioRxiv - Cell Biology 2023Quote: ... Swollen cells were then ruptured using pre-cooled dounce homogenizer and the tight pestle (VWR Cat. 62400-595). The ruptured cells were centrifuged at 218xg for 5 min to pellet nuclei ...
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bioRxiv - Immunology 2023Quote: ... at a density of 5 x 106 cells/mL in 100 μL of RPMI 1640 (#45000-396, VWR) supplemented with 10% fetal bovine serum (Omega Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Digested tissues were passed through 100 and 70 um nylon cell strainers (Corning, VWR# 10054-458, 89508-344). Single cell suspensions were washed by MACS buffer ...
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bioRxiv - Cell Biology 2023Quote: Frozen lentiviral stocks were generated by transfecting 70% confluent HEK-293T cells in 15cm cell culture dishes in ‘MEF medium’ together with a solution consisting of 1ml 150mM NaCl (1.06404.1000, VWR), 1ml Polyethylenimine (23966-1 ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded on a 24-well plate on 12 mm #1.0 glass coverslips (VWR, CAT# 631-1577P) in 500 µL media ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Cells and tissues were lysed by radioimmunoprecipitation assay (RIPA) buffer (0.2% IGEPAL CA-620, 0.02% SDS with VWR Life Science Protease Inhibitor Cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... coli (E23) CRISPRi cell lines (details below) were grown overnight to stationary phase in LB Lenox Broth (VWR) before dilution 1:50 into fresh media in the morning ± 2 µM anhydrotetracycline ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Physiology 2024Quote: ... cells were perfused with Ca2+-containing Krebs-Ringer’s solution supplemented with 0.1% fatty-acid free BSA (E588, VWR). To trigger ionic responses ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were separated from the culture liquid by centrifugation and filtration through 0.22 µm membranes (VWR, USA). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in 6-well plates after placing round coverslips in the bottom of the wells (VWR), or in PhenoPlates 96-well (Perkin Elmer) ...
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bioRxiv - Neuroscience 2024Quote: ... The solution containing dissociated neurons was passed through a 100 μm sterile cell strainer (VWR, Cat# 21008-950) into a 50 mL conical tube ...
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bioRxiv - Biophysics 2024Quote: Experiments were done using a flow cell assembled by separating silanized hydrophobic coverslips and cleaned glass slides (VWR) by double sided tape (3M) ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... at a 1:285 dilution in 96-well plates with optically clear plastic bottoms (VWR 10062-900). To avoid evaporation from the wells ...
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bioRxiv - Immunology 2019Quote: ... and plated at 1 ml/well in 24-well tissue culture plat (Falcon, VWR Edmonton, AB, Canada). Cells were stimulated with SLA (50 μg/ml ...
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bioRxiv - Systems Biology 2021Quote: ... 50 ng lysozyme and PMSF (final concentration of 1 mM) and sonication (Branson Sonifier 450, VWR international). Centrifugation was used to separate soluble proteins from cell debris ...
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bioRxiv - Neuroscience 2021Quote: ... 1:5000 dilution in PBS-T) for one hour and developed with chemiluminescent reaction (VWR, 490005-008).
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bioRxiv - Neuroscience 2021Quote: ... cOFM probes were used and the setup was prepared as follows: 1% Cold Sterilant (VWR #148-0062) was flushed for 30 min and washed out with Ampuwa sterile water (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were subcultured 1:50 (OD 0.05 ± 0.02) in LB medium in 2.2 ml 96-well deep well plates (VWR) and grown to exponential phase (3 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with an antibody against RFP (1:1000, Rockland (VWR), catalog #RL600-401-379) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Phage DNA was extracted using an equal amount of phenol:chloroform:isoamyl alcohol (25:24:1) (VWR Life Science) to lysate ...
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bioRxiv - Microbiology 2020Quote: ... and wine yeasts (Table 1.) and pre-cultured the products in YPD medium (VWR, Radnor, PA, USA) for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 5% CO2 at a density between 0.3-1 x 106cells/mL in RPMI medium (VWR 45000-396) topped up with 10% Fetal Bovine Serum (Genesee Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton X) containing a cocktail of protease and phosphatase inhibitors using a bead disruptor (Omni, VWR). Homogenates were centrifuged at 2100g for 10 min at 4°C and supernatants stored at −80°C ...
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bioRxiv - Bioengineering 2021Quote: ... Left ventricles from donors were sectioned and decellularized using 1% Sodium dodecyl sulfate (SDS, VWR, PA, USA) for 24 h or until the tissues turned transparent white ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Cancer Biology 2022Quote: ... Organellar pellet was then washed twice and resuspended in 100μL of 1% Triton X-100 (VWR, 0694) in 50mM Tris-HCl and 150mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The micropipette was then sandwiched between a 1-mm glass cover slide (3”x1”x 1mm; VWR) and a #1.5 glass cover slip (24×60 mm ...
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bioRxiv - Microbiology 2022Quote: ... prepared as a 1:5 dilution in buffer (pH=7.2) made using Gurr buffered tablets (VWR #331942F) and filtered ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were mounted on micro-scope slides using 4:1 Citifluor:Vectashield solution (VWR and Vector Laboratories) and stored at −20 °C ...