Labshake search
Citations for VWR :
1401 - 1450 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: RNA was isolated from tissue or cell culture using the EZNA RNA isolation Kit (VWR, Spånga, Sweden) or using TRIzol reagent and RNeasy Universal Mini Kit (Qiagen ...
-
bioRxiv - Neuroscience 2020Quote: LUHMES cells and neurons were grown on coverslips (No. 1.5 round-shaped borosilicate glass, VWR 631-0150) pre-treated with concentrated hydrogen chloride (37%) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cultured in an optimized mouse prostate primary cell medium composed of DMEM/F12 (VWR, 45000-344), 10% FBS ...
-
bioRxiv - Cell Biology 2022Quote: HEK293T cells were plated on poly-D-lysine coated 24-well plates (356413, Corning BioCoat, VWR, UK) and transfected with 2.4 μg of pEF-zDHHC2 plasmid (or the control empty plasmid pEF-BOS-HA ...
-
bioRxiv - Immunology 2022Quote: ... resuspended with complete DMEM medium and filtered through a 70-μm cell strainer (VWR international, 10199-657). Bone marrow progenitor cells were cultured in 100 mm dishes for 6-7 days in 70% complete DMEM medium and 30% L929-conditioned medium ...
-
bioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells were plated at a confluency of 70% on glass cover slips (VWR, Cat# 26022) that were acid washed and precoated with poly-D-lysine (0.1 mg/ml ...
-
bioRxiv - Synthetic Biology 2020Quote: ... An identical procedure was performed using LNCaP FLuc+ cells mixed with equal volume Matrigel (VWR, ON, Canada) to aid tumour formation [41] ...
-
bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
-
bioRxiv - Bioengineering 2021Quote: NIH-3T3 cells expressing GFP (ex/em 488/509) were maintained in a Symphony incubator (VWR, USA) at 37⍰C and 5% CO2 and grown in cell culture grade T-75 flasks ...
-
bioRxiv - Immunology 2021Quote: ... cells were resuspended in cold PBS/0.04% BSA and fixed by adding 4 volumes 100% Methanol (VWR) and incubation for 30 min at -20 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were briefly washed with PBS and then incubated in medium containing vehicle (DMSO; #97061-250, VWR) or indicated concentrations of digoxin (#D6003 ...
-
bioRxiv - Microbiology 2023Quote: ... After 72 h media were aspirated and cells were fixed in 10% neutral buffered formalin (NBF) (VWR) for 20 minutes (min) ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were detached with TrypLE enzyme and were fixed with 1.6% (v/v) PFA (VWR, # 100503 917) for 10 minutes at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were detached with TrypLETM Express enzyme and fixed with 1.6% (v/v) PFA (VWR, # 100503 917) for 10 minutes at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... media was extracted and passed through a 40 µm cell strainer (VWR, Radnor, PA, USA, 21008-949) for in vitro α-Syn spreading experiments (Fig ...
-
bioRxiv - Developmental Biology 2023Quote: ... the suspension was filtered through a 40 μm cell strainer (VWR, Radnor, PA, USA, Cat# 76327-098) to yield a single-cell suspension ...
-
bioRxiv - Bioengineering 2023Quote: ... 10,000 viable cells were plated per well in white polystyrene 384-well microplates (Greiner Bio-One; VWR). Cells were exposed to MRX-2843 and venetoclax (5 – 1,000 nM ...
-
bioRxiv - Bioengineering 2023Quote: ... deposited 10,000 viable cells per well (0.25×106/mL) in 384-well microplates (Greiner Bio-One; VWR). Cells were combined with drug matrices using a Beckman NX Liquid Handler (Beckman Coulter ...
-
bioRxiv - Biophysics 2023Quote: ... The cells were then washed with PBS and permeabilized with a 0.5% Triton-PBS (VWR, 0694-1L) solution for 20 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... and tissue was mechanically disrupted by beads to further lyse bacterial cell walls (VWR Mini Bead Mill).
-
bioRxiv - Cell Biology 2023Quote: ... resuspended cells were pipetted onto microscope slides in 7.5 μL aliquots (Fisherbrand™ Superfrost™ Plus, VWR: 48311-703 ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Total RNA from cells was isolated using the peqGOLD total RNA isolation kit (VWR, Radnor, PA, USA) according to the manufacturer’s protocol and RNA concentrations of the samples were determined with a NanoDrop 1,000 spectrometer (VWR ...
-
bioRxiv - Cell Biology 2023Quote: HEK293T cells were seeded at 225,000 cells/well onto 0.01 mg/mL poly-D-lysine coated coverslips (#1.5, VWR) in 6-well plates ...
-
bioRxiv - Developmental Biology 2023Quote: Cells were lysed after 12 h or 16 h using 500 μL pepGOLD TriFast ™ reagent (VWR) and stored at -80 until the RNA extraction process was resumed ...
-
bioRxiv - Cell Biology 2023Quote: Hippocampal neurons seeded at 180,000 cells/well onto 0.1 mg/mL poly-L-lysine coated coverslips (#1.5, VWR) in 6-well plates ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were transferred to FACS buffer (2mM EDTA, 0.5% BSA in DPBS) and filtered (VWR, 734-0001). For single cell RNA sequencing ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were fixed using 4% paraformaldehyde for 10 minutes on the rocker (VWR analog rocker 10127-876). Fixed cells were washes once with PBS ...
-
bioRxiv - Microbiology 2023Quote: Cells were grown to mid-log phase in SC medium in 96 deep-well plates (VWR, 82051). After washing with ddH2O twice and spinning down at 3,000g for 5 minutes ...
-
bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50μL of 1% (w/v) SDS (VWR International, Radnor, PA, USA). Colorimetric absorbance was measured at 414nm using a PowerWaveXS plate reader (Biotek ...
-
bioRxiv - Developmental Biology 2020Quote: ... the NTM was replaced with 1 mL NTM with 0.25 mg/mL NBT (VWR, 97061-412) and 0.125 mg/mL BCIP ...
-
bioRxiv - Genomics 2020Quote: ... 3 15min washes in 5x SSCT (1:10,000 DAPI, VWR, TCA2412-5MG, in the second wash), and storage/imaging in 5x SSCT ...
-
bioRxiv - Biophysics 2020Quote: ... PDMS (base to curing agent ratio of 10:1 w/w; #634165S, SYLGARD 184, VWR, Germany) was poured over the master ...
-
bioRxiv - Bioengineering 2021Quote: ... and secondary antibodies were diluted to 1:10,000 (100 ng/mL) in 2.5% BSA (0332, VWR) in Tris buffered saline tween ...
-
bioRxiv - Bioengineering 2022Quote: ... 350μL of a 40mg/mL−1 solution of bovine serum albumin (BSA) (AAJ64100, VWR, PA, USA) in PBS (Thermo Fisher ...
-
bioRxiv - Evolutionary Biology 2019Quote: 1 ml of each lysate was first treated with 12.5 μl 1M MgCl2 (VWR Life Science), 0.4 μl DNAse 1 (2000 U/ml ...
-
bioRxiv - Bioengineering 2020Quote: ... 1-(3-(Dimethylamino)propyl)-3-ethylcarboiimide hydrochloride (EDC-HCL; 0.5 g, 2.6 mmol, 1.02 equiv., VWR) and NHS-ester (0.65 g ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were loaded on a 1% agarose gel and stained with EZ-Vision® (VWR). Images were taken using the GelDoc system (Bio-Rad).
-
bioRxiv - Molecular Biology 2021Quote: ... rose tissue was macerated in 1 mL of phosphate buffer saline (1X PBS) (VWR, Radnor, PA), 0.05% Tween 20 ...
-
bioRxiv - Genomics 2021Quote: ... 3 15min washes in 5x SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5x SSCT ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... transferred to an ~1 cm square agar “pad” on a glass microscope slide (VWR; 16004-422) and covered with a glass coverslip (VWR ...
-
bioRxiv - Microbiology 2019Quote: ... in tap water (pH adjusted to 7.2 with NaOH) + 1% (w/v) glucose (VWR International, USA) containing 100 μg/mL ampicillin (E ...
-
bioRxiv - Physiology 2022Quote: ... Total RNA (1 μg) was reverse transcribed using qScript cDNA Synthesis Kit (VWR, Houston, TX, USA). qRT-PCR analysis was performed using SYBR Green qPCR Master Mix (VWR) ...
-
bioRxiv - Immunology 2022Quote: ... The reaction was terminated with 50μL of 1% (w/v) SDS (VWR International, Radnor, PA, USA). Colorimetric absorbance was measured at 414nm using a PowerWaveXS plate reader (Biotek ...
-
bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
-
bioRxiv - Cancer Biology 2023Quote: ... the peptides were re-dissolved in 10 μl 1% formic acid (VWR Ltd., Radnor, PA, USA) and were separated in a 180 min water/acetonitrile gradient using an Easy nLC 1200 nano UPLC (Thermo Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... RRID:AB_2259284) in a 1:500 dilution in PB containing 0.4% Saponin (VWR International AS, Bergen, Norway) and 5% normal goat serum ...
-
bioRxiv - Microbiology 2023Quote: ... Pellet was resuspended in 1 mL of Dulbelcco’s Modified Eagle’s Medium (DMEM) (Corning; VWR 45000-304). This suspension was diluted 10-fold by adding 500 uL to 4.5 mL of DMEM to a final concentration of 1×106 cells/mL ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were treated for 4 h with 4HC or doxorubicin at IC50-range drug concentrations using cells seeded the prior day at 50,000 (E0771) or 75,000 cells per well (4T1, B16F10) of a 6-well plate (cat. #10861-696, VWR). Cells were then washed once with PBS and incubated in fresh media for a total of 24 ...
-
bioRxiv - Genetics 2020Quote: ... the egg-containing plug-in cells were removed from the plates and fixed vertically on petri dishes (VWR) with plastiline (Pelikan Schindellegi ...
-
bioRxiv - Molecular Biology 2021Quote: ... we transfect the cells with either WT or mutant SOD1 pCI-Neo plasmid using JetOptimus transfection reagent (VWR) according to the manufacturer’s instructions (31) ...