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Citations for VWR :
1351 - 1400 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: BJ-5ta cells were lifted from T-150 culture flasks using 0.25% trypsin (VWR 45000-664) for 5–10 min ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were seeded into 96-well Flat Bottom TC treated culture plates (VWR® 10062-900) at a density of 15,000 cells per well in F-12K medium with 10% FBS for 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... SMS-CTR and Rh36 cells were maintained in RPMI supplemented with 10% fetal bovine serum (VWR) at 37° C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then manually homogenized with 20 strokes of a PTFE head Tissue homogenizer (VWR®) and centrifuged at 1000xg for 10 minutes ...
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bioRxiv - Immunology 2019Quote: ... All of the cells were cultured in humidified incubators at 37 °C with 5% CO2 (VWR).
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bioRxiv - Microbiology 2020Quote: ... Fungal mycelium was collected using cell strainers with 40 μm mesh size (VWR TM Darmstadt, Germany) and washed with protoplasting buffer (0.1 M sodium citrate ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were incubated at 30°C for 18 h in a stable incubator (INCU-Line, VWR), unless otherwise stated ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were harvested and washed with Wash medium (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) twice and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were generated in ice cold RIPA lysis buffer (20mM Tris-HCl (VWR, 97061-258), pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... diatom strains were grown in 25 cm2 or 75 cm2 cell culture flasks (VWR Avantor, USA), in 17 ml or 50 ml f/2 medium ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded onto polycarbonate transwell inserts with pore size of 0.4 µm (VWR, United Kingdom) at the recommended seeding density (CELLnTEC ...
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bioRxiv - Molecular Biology 2022Quote: DNA was extracted from HEK-293 cells using DirectPCR® DNA Lysis Reagent (VWR, 732-3255) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated at low confluency on ethanol-cleaned 25 mm #1.5 thickness round coverglass (VWR) for imaging ...
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bioRxiv - Microbiology 2022Quote: ... All cells were maintained at 37 °C in a 5% CO2 humidified incubator (VWR, Mississauga, Ontario). For subculturing ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos were collected with a paintbrush and water into a cell collection chamber (VWR #732-2758). These baskets were placed in a 50% bleach solution for dechorionation and agitated with a pipette ...
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bioRxiv - Molecular Biology 2023Quote: 750K B16-HG cells were seeded in 25cm2 plug seal cap flasks (VWR, cat: 82051-070) at the beginning of the time course ...
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bioRxiv - Genomics 2023Quote: ... HeLa cells in the conventional media screen were cultured in DMEM (VWR International LL #45000-304) supplemented with 10% dialyzed FBS (ThermoFisher Scientific #26400044) ...
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bioRxiv - Microbiology 2023Quote: ... coli cells were grown at 37 °C with aeration in either LB (Luria-Bertani broth; VWR) or LA (Luria Agar ...
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bioRxiv - Immunology 2023Quote: ... The cells were fixed by dipping 10 times in pre-cooled ice-cold acetone (VWR, #20066.296) in a glass tray ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysis from organoids and co-culture assays was performed with peqGOLD TriFast (VWR Life Science) as recommended by the manufactures followed by RNA isolation with the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in a primary antibody (CD63 for the endosomal system, clone MX-49.129.5, VWR, anti-LAMP1 for lysosomes ...
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bioRxiv - Immunology 2023Quote: 6 × 104 cells were seeded onto the glass part of P35 confocal dishes (75856-742, VWR), treated ...
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bioRxiv - Biochemistry 2023Quote: ... cells were fixed and stained using crystal violet and scored with a colony counting pen (VWR).
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bioRxiv - Cancer Biology 2023Quote: ... Colonies formed by tumor cells resistant to 6-Thioguanine were fixed with 4 % Formaldehyde (VWR Chemicals) and stained with Hematoxylin Harris (VWR Chemicals) ...
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bioRxiv - Microbiology 2024Quote: ... The platelet (bacterial cells) was then lysed by sonication using a 250HT ultrasonic cleaner (VWR International), set at 60 Hz for 20 min in the same buffer ...
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bioRxiv - Immunology 2023Quote: ... as described [32] The solution was then filtered through a 100 µm nylon cell strainer (VWR) and centrifuged at 400 x g for 10 minutes at 4°C and the supernatant was discarded ...
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bioRxiv - Cancer Biology 2024Quote: ... cells expressing the various shRNAs were selected with 0.2 mg/ml hygromycin (cat#K547-20ml, VWR) at 72-hours post transfection and maintained at 0.8 mg/ml of hygromycin after cell line purification.
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bioRxiv - Immunology 2024Quote: ... The tissue suspension was then passed through 40 µm cell strainer (VWR international, Randor, PA, USA) and the cell suspension was collected ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% non-fat milk and 1% BSA filtered through a paper filter (qualitative, 415, VWR). Blots were rinsed 1-2 times with Wash Buffer ...
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bioRxiv - Immunology 2019Quote: ... DRGs were immediately digested with 1 mL of papain solution (HBSS/10 mM HEPES (VWR)/500 μM EDTA (Westnet)/0.4 mg/mL L-Cysteine (Sigma)/1.5 mM CaCl2 (Sigma)/20 unit/mL Papain (Worthington) ...
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bioRxiv - Biophysics 2021Quote: ... Two no.1 cover slides (24 × 60 mm and 18×18mm, VWR, Radnor, Pennsylvania, USA) are cleaned by sonication in isopropanol and dried under a stream of N22 ...
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bioRxiv - Cell Biology 2022Quote: Liver (∼100mg) was homogenized in 1 mL of PBS using a Bead Mill Homogenizer (VWR). Lipids were extracted ...
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bioRxiv - Molecular Biology 2020Quote: ... zebrafish embryos were homogenized in 1 mL PBS and shaken in a glass vial (VWR) with 1 mL methanol and 2 mL chloroform containing internal standards (13C16-palmitic acid ...
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bioRxiv - Microbiology 2020Quote: ... Teichoic acids were removed by resuspending the samples in 1 ml 49% hydrofluoric acid (VWR) rocking for 48 hrs 4°C ...
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bioRxiv - Immunology 2020Quote: ... the culture medium was replaced with 1 mL growth medium supplemented with G418 (Geneticin; VWR) antibiotic to a final concentration of 250 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 15 mm Ø No.1 coverslips and hydrochloric acid (HCl) 37% were purchased from VWR International (Radnor ...
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bioRxiv - Synthetic Biology 2023Quote: ... then planted onto YPM agar supplemented with 100 µg mL-1 kanamycin (kan) (IB02120, VWR). Recombinants were selected and grown overnight in YPM supplemented with 100 µg mL-1 kan ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of swollen conidia was aliquoted into a 5 mL screwcap centrifuge tube (VWR). To each 1 mL aliquot ...
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bioRxiv - Microbiology 2023Quote: ... extracted biofilm matrix was lyophilized using an ALPHA 1-4 LDplus freeze dryer (Christ, VWR) and kept at -80 °C until sample preparation for proteomics.
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bioRxiv - Neuroscience 2023Quote: ... PDMS at a 10:1 ratio (w/w; base:curing agent; Sylgard 184 silicone elastomer, VWR) was cast into a 3D printed square shape mold and cured at 55 °C for 24 h to make PDMS support ...
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bioRxiv - Biophysics 2023Quote: ... PDMS (base to curing agent ratio of 10:1 w/w; VWR 634165S, SYLGARD 184) was poured over the master ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414). Technical replicates of 20 ng (based on RNA concentration ...
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bioRxiv - Neuroscience 2021Quote: ... 10% FBS)) and filtered through a 100 uM cell strainer (cat no. 21008-950, VWR, Radnor PA). Cell density was quantified using a Countess II Automated Cell Counter (cat no ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: Wilt-type and ISL2 KO cells were plated in micro cover glasses (VWR cat. no. 48368-062) at a density of 2×104 in regular growth conditions ...
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bioRxiv - Cell Biology 2020Quote: ... except cells were disrupted with 20 strokes in a homogenizer containing a pure PTFE head (VWR International) and two strokes with a dounce tissue grinder with tight-fitting pestle (DWK Life Sciences Kimble Kontes) ...
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bioRxiv - Biochemistry 2020Quote: ... Plasma was aspirated off and the cells were resuspended in RPMI-1640 without glutamine (VWR, VWRL0106-0500) supplemented with 5mM L-glutamine (Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Drosophila melanogaster S2R+ cells were maintained in Schneider’s Insect Medium supplemented with 10% heat inactivated FBS (VWR) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Cell aggregates on top of the droplets were transferred to an electroporation cuvette (4 mm gap, VWR). Three square wave pulses of 1 kV for 70 μs were applied with 1 s intervals between each pulse using an ECM 2001 Electro Cell Manipulator (Harvard Apparatus ...
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bioRxiv - Physiology 2019Quote: ... cells cultured in a 96-well plate were incubated with MTT reagent (5mg/ml, VWR, Radnor, PA) for the final 90 minutes of AGE treatment ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from cells using the EZNA Total RNA Kit (Omega, VWR, Radnore, PA, USA). 1 μg of RNA was reverse-transcribed into cDNA using the RT Omniscript cDNA kit (Qiagen) ...