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Citations for VWR :
1301 - 1350 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Cells were seeded in a 96 well flat-bottom tissue culture plate (VWR, Radnor, PA) at 6,000 cells per well in 250 μL of EGM2 and were incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were detached with TrypLETM Express enzyme and fixed with 1.6% (v/v) PFA (VWR) for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... the culture was terminated by either fixation of the cells with ice-cold methanol (VWR) for immunocytochemistry (ICC ...
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bioRxiv - Cell Biology 2024Quote: ... HL-60 cells were diluted into RPMI-1640 medium containing 1.3% DMSO (VWR Life Science) with an initial density of 1*106 cell/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS and HeLa cells were seeded in 24-well plate on #1.5 glass coverslips (VWR), irradiated and/or treated 24 h later ...
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bioRxiv - Molecular Biology 2024Quote: ... MEL015_DTres cells were cultured in 6-well plates containing round coverslips in the bottom (VWR) and were transfected with an ASO targeting ROSALIND or a scramble ASO control as previously described ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were afterwards washed several times in quencher solution [10 mM sodium ascorbate (VWR), 10 mM sodium azide (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... Expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, 437144N) at 26 °C for 6 h ...
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bioRxiv - Microbiology 2020Quote: ... or after 10-1 dilution in molecular biology grade ddH20 (VWR Chemicals, Darmstadt, Germany) for specific amplification and high throughput sequencing of the fungal rDNA internal transcribed spacer 1 (ITS1) ...
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bioRxiv - Microbiology 2019Quote: ... 1 U of VWR Taq DNA Polymerase (VWR®, WR International bvba/sprl, Belbium), 1 X key Buffer supplemented with MgCl2 (1.5 mM) ...
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bioRxiv - Bioengineering 2020Quote: ... Both methods of surface modification were applied to 1” x 3” glass slides (VWR). A small volume (1.75 µL ...
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bioRxiv - Neuroscience 2020Quote: ... cooled down with −1 °C/min in a cryo container (Mr. Frosty™, VWR) and stored at −80 °C or in liquid nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... an equal volume of 25:24:1 phenol-chloroform-isoamyl alcohol (VWR, Radnor, PA) was added and the tubes were vortexed for 30 s followed by centrifugation at 10,000g for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by a washing step with 3 mL 1% acetonitrile (ACN; VWR, 83640-290)/0.1% TFA and elution with 500 µL 40% ACN and 500 µL 60% ACN ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked at room temperature for 1 h using Rockland blocking buffer (VWR). Primary PDI antibody (Abcam ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Colon tissues were homogenized for 1 minute using a disperser (T10 ULTRA-TURRAX; VWR) on ice ...
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bioRxiv - Microbiology 2019Quote: ... A total of 1 x 108 CFU was added to fresh sheep blood (VWR) in V-bottom 96-well plates (Corning) ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1 hour, washed with Wash media (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-cavin3 (Cat no. ABNOH00112464-MO4, 1:200 PLA, VWR International Pty, Australia), rabbit anti-BRCA1 C-20 (SC-642 ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 ml of pre-chilled (−20°C) methanol/acetic acid (3:1, VWR chemicals) was added dropwise and flicking to homogenize ...
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bioRxiv - Immunology 2020Quote: ... Plates were then blocked with 100μl of blocking buffer (1% Casein in PBS; VWR) for 1.5hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... to obtain a final concentration of 1 μM and sodium nitrate (SS0680-500GR VWR) to obtain a final concentration of 10 μM.
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bioRxiv - Biophysics 2022Quote: ... induced with 1 mM B-isopropyl-thiogalactoside-non-animal-origin (IPTG-NAO; VWR USA) in the early-mid log phase ca 1.5 h or OD600 of 0.6 and incubated for another 5 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... by injecting 50 μl of substrate buffer (lysis buffer+ 1 mM DTT (VWR Scientific), 1 mM ATP (Sigma Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... and was stopped by adding 1 μL of 18 mM EDTA (VWR, 97062-656) to each well ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were blocked for 1 h with Rockland blocking buffer (VWR, Catalog #RLMB-070) then incubated with PDI antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:50 in 5 % BSA/TBST overnight at 4°C (BSA: VWR; #422361V). As a negative control ...
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bioRxiv - Immunology 2023Quote: ... 1 × 107 hepatocytes were mechanically disrupted with a pellet mixer (VWR Radnor, PA, USA) for 5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: 1,5 x106 PBMCs were seeded in 1 ml RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction was quenched by adding 70 µL 1% formic acid (VWR, Radnor, PA) containing 0.1 µg/mL isotope-labelled peptide Ac-KKTIPNDSR*E (Cambridge Peptides ...
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bioRxiv - Systems Biology 2024Quote: ... Afterwards beads were rinsed twice with 1 mL 80% (v/v) ethanol (EtOH, VWR) and once with 800 µL ACN ...
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bioRxiv - Synthetic Biology 2024Quote: ... Supernatants were discarded and the pellets were then resuspended in 1 mL acetone (VWR), followed by adding 200 μL glass beads (Sigma) ...
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bioRxiv - Synthetic Biology 2024Quote: ... LB agar was prepared by dissolving 37 g L−1 LB agar powder (VWR) into required amount of distilled water ...
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bioRxiv - Biophysics 2021Quote: ... The cells were left for 7 days and then fixed using 100% methanol (MeOH) (VWR, BDH2029) and stained with a crystal violet (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed with 30 passes through a tissue homogenizer (VWR Cat. No. 89026-386), followed by pelleting nuclei and un-lysed cells for 3 min at 3,000×g to obtain a post-nuclear supernatant (PNS) ...
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bioRxiv - Physiology 2022Quote: ... cells grown on coverslips were washed with PBS and fixed with 4% paraformaldehyde (VWR Chemicals, Spain). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... 104 cells in culture medium were mixed with 0.4% low melting point agarose (VWR, 89125-532) at 370C and seeded on top of 1% agarose standing layer in 12 well dishes ...
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bioRxiv - Genomics 2020Quote: ... Cells were spun at 350g for 15min onto 24 well glass bottom plates (VWR, 82050-898) coated with WGA (VWR ...
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bioRxiv - Molecular Biology 2019Quote: ... Completed DMEM was added to inactivate trypsin and cells were collected into 15 mL tubes (VWR). The tubes were centrifuged at 300 rcf for 5 minutes to pellet cells and total protein was extracted by resuspending each pellet in 1 mL of RIPA buffer (150 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... and the sample was then passed through a 40 µm cell strainer (Cat. # 10199-655, VWR) into a 50 mL conical tube on ice ...
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bioRxiv - Neuroscience 2020Quote: ... the lysate was transferred to a 100 μm nylon cell strainer (VWR, ref nr 732-2759) to remove tissue debris and the flow-through was centrifuged at 2600 × g at 4 °C for 1 min to pellet nuclei ...
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bioRxiv - Microbiology 2020Quote: ... the cells were sorted into sterile 96-well tissue culture plates (VWR, item no. 10062-900) by using the single cell sorting option at a pressure of 60 psi on a MoFlo Astrios Flow Cytometer (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Growth curves were acquired by following OD with a Biowave cell density meter CO8000 (VWR International). Growth rates were inferred by fitting growth-curves to OD as a function of time with a four-parameter logistic function was used70.
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bioRxiv - Microbiology 2021Quote: ... Growth curves were acquired by following OD with a Biowave cell density meter CO8000 (VWR International). Supernatant samples were collected and processed as explained above ...
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bioRxiv - Biophysics 2020Quote: ... All cells used in this study tested mycoplasma negative using the Lonza MycoAlert (VWR 75870-454) testing system.
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bioRxiv - Cell Biology 2020Quote: ... PSCs were seeded single-cell on Corning® Matrigel® Matrix-coated plates (VWR, Leuven, Belgium) at a density of 50,000 cells/cm2 ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia were plated at 7×106 cells/well in 6-well plates (VWR #10062-894) and incubated at 5% CO2 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were permeabilized for 30 minutes at room temperature with 0.3% Triton-X (VWR 80503-490) in PBS ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells expressing Cas9 were selected by 350 ng/ml Puromycin dihydro-chloride (VWR International GmbH, #J593) for three consecutive days starting one day after electroporation (Steyer et al. ...