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Citations for VWR :
1551 - 1600 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
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bioRxiv - Developmental Biology 2022Quote: ... The agarose cubes containing forelimbs were submerged in 1 mL solution containing 0.05% sodium dodecyl sulfate (SDS; VWR), with 2% penicillin-streptomycin or 0.02% sodium azide (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... 7-day-old seedlings grown on hydroponic media were mounted on coverslips (24×50 mm No. 1½, VWR) using medical adhesive (Stock No ...
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bioRxiv - Plant Biology 2020Quote: ... The AIR was digested with 1 mL ultrapure 70% nitric acid (BDH Aristar® Ultra, VWR, Radnor, PA) in 15 mL Teflon beakers ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µl cells were mounted onto microscope slides containing a thin layer of 1 % agarose in water (supplemented with 10 % medium, where appropriate) and covered with 0.13-0.17 mm coverslips (VWR). Nucleoids were visualised using 1 µg/ml DAPI (Sigma Aldrich ...
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bioRxiv - Physiology 2021Quote: ... through sieves with different mesh sizes (4, 3.15, 2.5, 1.25 and 1 mm, VWR International GmbH, Darmstadt/Germany). Flowthrough of the 1 mm sieve was collected in a pan ...
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bioRxiv - Neuroscience 2021Quote: ... In short, free-floating sections were blocked (5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) and then incubated with different primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... eyes enucleated and fixed for 1 h in 4% paraformaldehyde (CAS: 50-00-0, Cat.No.: 100504-858, VWR)) in PBS at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... which consisted of RPMI-1640 medium supplemented with 1% Ultra Low IgG Fetal Bovine Serum (VWR 4100-050) and added to the cell-containing wells ...
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Synthetic Biology 2020Quote: ... 10 µL was quickly pipetted onto a 20 x 20 mm #1 cover glass (VWR, Radnor, PA, USA) and inverted onto a glass slide before the gelation reaction was completed ...
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bioRxiv - Biochemistry 2020Quote: ... Blocks of around 1 mm2 were cut and cryo-protected by overnight infiltration in 2.3 M sucrose (VWR) in 0.1 M phosphate buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We filter-sterilized the supernatants by transferring them to 96-well 1 mL filter-plates (VWR International, Switzerland), followed by centrifugation at 1200 rpm for 15 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then permeabilized in 1× PBS with 0.25% TritonTM X-100 (Cat. No. 97063-864, VWR) and FBS for 20 min ...
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bioRxiv - Biochemistry 2020Quote: α-Syn (1 mg/mL) was dissolved in 10 mM NaOH and sonicated (VWR® Symphony™ Ultrasonic Cleaner) for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of conditioned medium was combined with 395 μl of premixed TCA (256 µl VWR Life Science) and 1% Triton-X 100 (139 µl ...
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bioRxiv - Developmental Biology 2023Quote: ... mice were weighed 1 hr prior to harvest and injected with 0.04 µmol/g puromycin (VWR, #75844-852).
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bioRxiv - Biophysics 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Briefly, HEK293T cells (ATCC catalog #CRL-3216, RRID:CVCL_0063) were grown in DMEM supplemented with 10% FBS (VWR, catalog #97068-085) and 1% penicillin-streptomycin ...
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bioRxiv - Genomics 2020Quote: ... Cells expressing Cas9 were selected either by sorting for GFP10 or selection with 350 ng/ml Puromycin dihydrochloride (VWR J593) for three consecutive days starting one day after electroporation17 ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator ...
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bioRxiv - Neuroscience 2020Quote: ... penicillin/streptomycin and amphotericin B (1X Anti/Anti, #15240062, TFS) (referred to as ‘SHM-10’) on uncoated cell culture flasks (VWR). Cultures were maintained in a sterile incubator (MCO-20AIC ...
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bioRxiv - Cell Biology 2021Quote: ... then lysed cells in 1mL of ice-cold nuclear isolation buffer in a 2mL Dounce homogenizer (VWR, cat. #62400-595). We next filtered the homogenate through a 40µm Flowmi cell strainer (WVR ...
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bioRxiv - Biochemistry 2021Quote: For transductions VeroE6 or HEK cells were seeded into greiner CELLSTAR® 96-well plates or 6-well plates (VWR) containing lentivirus in DMEM containing 2 % FBS and 1 μg/mL polybrene ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris and unbroken cells were removed by centrifugation and supernatants were passed through a 0.22 μm filter unit (VWR International). Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μl of cells were put to 180 μl of TYI-S-33 media in 96-wells Tissue Culture Plate (VWR) and the same dilution step was performed 5 times ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PC3-GFP KPC and NIH-3T3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, cat. no. 16777-129, VWR international, LLC.) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... clear-bottomed, 96-well plates (Falcon, Switzerland) at a density of 200,000 cells/mL or onto coverslips (CS) (VWR, Switzerland) at a density of 250,000 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) and were mounted onto Superfrost Plus microscope slides (VWR) in VECTASHIELD antifade mounting medium (Vector Laboratories) ...
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bioRxiv - Genetics 2019Quote: ... Four million S2 cells that were stably transduced with RasV12Common and RasV12Rare plasmids were seeded into coated tissue culture dishes (60×15mm, VWR) with 2 ml of Schneider’s media (without FBS) ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Cell Biology 2021Quote: ... Cell suspension was filtered through 35 μm nylon filter cap on 12x75 mm round bottom tubes (VWR/Falcon 21008- 948). Flow cytometric measurements were performed using a BD LSR Fortessa ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were seeded on 24-well plate (0.5 x 105 cell/well) in growth medium (DMEM medium; Thermo Fisher Scientific, 10569010, fetal bovine serum; VWR, 97068-085 ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The supernatant from the second and third digestions were then pooled and strained through a 100 μm cell strainer (VWR). Pooled and digested villous suspensions were then centrifuged at 4°C at a speed of 1200 rpm for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Peripheral blood mononuclear cells (PBMC) from 5 healthy young adult donors (mean age 28) were isolated by Ficoll-Paque (VWR) density centrifugation and stained with fixable viability dye efluor506 (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: Hypothalamic progenitors differentiated from the HUES9-POMC-GFP cell line were re-plated onto 24 mm round glass coverslip (VWR) that has been pre-treated with 5 M hydrochloric acid and double coated with both 0.01% poly-L-ornithine hydrobromide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Germany) using standard protocols and transferred to glass-bottom cell culture chambers (cat#631-0150, VWR International, Radnor, PA, USA). 24 h post gene silencing ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and 10 ml of serum-free DMEM containing 0.2% w/v bovine serum albumin (BSA; VWR international) and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting material was well resuspended with keratinocyte media and then filtered through a 70 μm cell strainer (VWR, Corning). The cell suspension was then centrifuged for 7 min at 4 °C 1500 rpm and washed twice with PBS ...
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bioRxiv - Cell Biology 2022Quote: Cells were plated onto 18 or 22 mm-glass coverslips (No. 1.5, 22×22 mm glass diameter, VWR 48366-227) the day before nutrient stress ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were seeded at a density of 45,000–55,000 cells onto 18-mm-diameter #1.0 round coverslips (VWR, Radnor, PA, USA) in 12-well dishes ...
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bioRxiv - Microbiology 2023Quote: Once primary airway epithelial cells reached ∼80% confluence, they were cultured on collagen coated cell culture inserts (Transwell®, 24-Well, 0.4 μm PET clear) (VWR) as previously published (Moreni et al. ...