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Citations for VWR :
1251 - 1300 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and exposed for 1–3 min to maximum sensitivity film (VWR, Radnor, PA). Broad-range pre-stained SDS–PAGE molecular weight markers (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were rehydrated into PBS + 1% Triton X-100 (PBTr) (VWR #97063-864) and incubated with ice-cold acetone at -20C for 20 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed in 1% (wt/vol) sodium dodecyl sulfate (SDS) (VWR, #97062) for 24 hours or until white transparent tissue was obtained ...
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bioRxiv - Synthetic Biology 2024Quote: Yeast extract Peptone Dextrose (YPD) media (10 g L−1 yeast extract (VWR), 20 g L−1 peptone (VWR) ...
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bioRxiv - Physiology 2024Quote: ... tissues were homogenized in 1 mL of RiboZol (Cat no. N580, VWR, USA), and then total RNA was precipitated in 500 μL of isopropanol ...
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bioRxiv - Physiology 2024Quote: ... Western blot secondary antibodies included: donkey anti-rabbit HRP (VWR 95017, 1:1000) and sheep anti-mouse (GE Healthcare #NA931V ...
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bioRxiv - Immunology 2024Quote: ... then blocked with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 hours at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was isolated from the cells with Zymo Quick-RNA Miniprep kit (VWR, #76299-632) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellets were re-suspended in 500 μl of 5 mg/ml lysozyme (VWR) and incubated for 20 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Astrocytes were seeded at a density of 1x105 cells on 12 mm glass coverslips (VWR) coated with poly-L-lysine ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were seeded at a density of 4x105 cells in 24 mm glass coverslips (VWR) coated with poly-L-lysine ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed in phosphate-buffered saline (PBS) and stained with 0.1% Crystal Violet (VWR) in 20% methanol ...
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bioRxiv - Genomics 2020Quote: ... two million cells were resuspended in 100 μl cold BTXpress electroporation solution (VWR 732-1285) with 20 μg Cas9 ...
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bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 4 µg DNA: 4 µL JetOPTIMUS transfection reagent (VWR, 76299-634) according to manufacturer guidelines in standard medium overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell suspension is passed through 40um pore size strainer (Falcon®, VWR 734-0004), and the strainer is washed by adding 2ml of accutase on the strainer ...
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bioRxiv - Neuroscience 2019Quote: ... The cell suspension was subsequently lysed using a probe sonicator (Branson Sonifier 250, VWR Scientific) on ice ...
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bioRxiv - Biochemistry 2021Quote: ... hippocampal neurons (85000 cells/ml) were plated on poly-D-lysine-coated glass coverslips (VWR) in 12-well plates (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... sections were transferred to 0.4 μm polycarbonate membranes cell culture inserts (VWR, Radnor, PA, USA) and incubated in Claycomb medium supplemented with 10 % foetal calf serum ...
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bioRxiv - Plant Biology 2020Quote: ... Spores were rinsed from their storage buffer using a 40 µm nylon cell strainer (VWR) by five washes with sterile UHQ water ...
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bioRxiv - Microbiology 2021Quote: ... cells were immobilised under agarose pads prepared using microarray slides (VWR, catalogue number 732-4826) as described in de Jong et al. ...
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bioRxiv - Immunology 2021Quote: ... Red blood cells in the spleen and thymus were lysed with ACK Lysing Buffer (VWR) or RBC Lysis Buffer (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... Cells were grown in 600 μL of medium in 96-well deep well plates (VWR). Plates were sealed with a gas-permeable microplate adhesive film (VWR ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in PBS and were mounted on VistaVision glass microscope slides (VWR) using Prolong Gold antifade reagent (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... 5 × 105 cells were cytospun onto 22 × 60 mm #1.5 coverslips (VWR international, Batavia, IL), pre-coated with CellTak (Corning) ...
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bioRxiv - Cell Biology 2022Quote: The cells were grown in 125 mL polycarbonate baffled shake flasks (VWR, Catalogue # 89095-258) at an agitation speed of 120 revolutions per minute (rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell suspension is passed through 40um pore size strainer (Falcon®, VWR 734-0004), and the strainer is washed by adding 2ml of accutase on the strainer ...
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bioRxiv - Bioengineering 2020Quote: ... we split the cell cultures into 96-well deep-well plates (Polypropylene 2.2 mL, VWR), where each well contained 0.5 mL cell culture ...
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bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were obtained by dissociating tumors in Kontes tubes with disposable pestles (VWR) followed by enzymatic dissociation in 200 U/ml collagenase IV (Worthington) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed three times in PBS and mounted on microscope slides (VWR International) in Prolong Gold (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed in 2X saline-sodium citrate (SSC) buffer containing 10% deionized formamide (VWR) and hybridized with fluorescently labeled oligonucleotide probe mixture for 4-16 hours at 37°C in hybridization buffer (2X SSC ...
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bioRxiv - Immunology 2021Quote: ... at 5×106 cells/mL by slow freezing in a Coolcell LX (VWR #75779-720) overnight in a −80°C freezer followed by transfer to liquid nitrogen.
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bioRxiv - Cell Biology 2022Quote: ... cells were filtered with Acrodisc WBC Syringe Filter 25 mm (VWR, Mont-Royal, QC, Canada) to isolate enucleated cells ...
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bioRxiv - Immunology 2022Quote: ... T cells from tumors were isolated with a 40/70 percoll gradient (VWR 17-0891). When sorted for RNA sequencing ...
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bioRxiv - Neuroscience 2023Quote: Peripheral Blood Mononuclear Cells (PBMCs) were cultured in medium QBSF-60 (VWR, Radnor, PA, USA) contains 10 µL/mL ascorbic acid ...
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bioRxiv - Cancer Biology 2023Quote: Cells were pelleted and then lysed in 1x RIPA Lysis Buffer (VWR Amresco #N653-100mL) supplemented with 1:100 protease inhibitor cocktail (Thermo Fisher #78440 ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysates were completely transferred to polycarbonate tubes and centrifuged (Micro Star 30, VWR) at 25,000 × g ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were selected for five days in presence of 3 μg/ml puromycin (VWR, CAYM13884). A near 100% GFP-positive population was confirmed via microscopy (ZOE Fluorescent Cell Imager ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were detached with TrypLETM Express enzyme and fixed with 1.6% (v/v) PFA (VWR) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked for 30 minutes room temperature with PBS+0.1% Triton X-100 (VWR) with 5% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were detached with TrypLETM enzyme and were fixed with 1.6% (v/v) PFA (VWR) for 10 minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... DAPI was added for 5 minutes and cells were mounted with Aqua PolyMount (VWR International) and a coverslip on the microslide ...
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bioRxiv - Bioengineering 2023Quote: ... The permeabilized cells were then blocked with 4% Bovine Serum Albumin (BSA) (VWR, PA, USA) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... BV2 cells were cultured in non-treated 10 cm tissue culture plates (VWR, #10062-880) in 5% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The cells were spread onto glass coverslips and attached to MicroSlides (cat#48311-703, VWR) using ProLong Gold antifade mountant (cat#P36934 ...
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bioRxiv - Immunology 2023Quote: ... All T cell cultures were maintained in RPMI medium containing 10% FBS (VWR, 97068–085) and 30 IU/mL human interleukin-2 (hIL-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell monolayers were then rinsed three times with PBS and fixed with 4% paraformaldehyde (VWR) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... spread onto LB agar plates with an L-shaped cell spreader (VWR# 220412261B, PA, USA), and incubated statically overnight at 37°C.
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bioRxiv - Bioengineering 2023Quote: ... the culture was terminated by either fixation of the cells with ice-cold methanol (VWR) for immunocytochemistry (ICC ...