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Citations for VWR :
1651 - 1700 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... THP-1 monocytes were differentiated into macrophages by treatment with 50 nM phorbol 12-myristate 13-acetate (PMA, VWR) for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1hr each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... samples were homogenized with a tissue grinder dounce (WHEATON® Dounce Tissue Grinder 1 mL, VWR, Radnor, Pennsylvania, USA) in 150 mM NaCl (S3014 Sigma-Aldrich - Merck ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day differentiated BMDMs isolated from control LysM-Cre+ or LysM-Cre+ Atg16L1loxp/loxp mice were plated at 10 x 106 cells per 15-cm non-TC treated dish (Petri dish, VWR, 25384-326) in BMDM media ...
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bioRxiv - Developmental Biology 2021Quote: ... for duck fibroblasts (i.e., CCL-141 cells) supplemented with 10% Fetal bovine serum (FBS, 97068-085, Lot# 283K18, VWR, Radnor, PA, USA) and 1X penicillin-streptomycin (15140122 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... cells were removed from their liquid media by flowing the liquid cultures that contained cells through a 0.45−μm pore filter (VWR, cellulose-acetate membrane). To ensure and verify that there were no cells remaining in the filtered media ...
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bioRxiv - Systems Biology 2020Quote: ... all subpools were combined into the final mutant collection pool by pipetting the subpools through a 100 μm cell strainer (VWR 10054-458). The final pool was mixed using a magnetic stir bar ...
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bioRxiv - Cancer Biology 2020Quote: 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: 3 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-742). The next day ...
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 2000 cells per well in a volume of 100 µl were seeded on flat-bottomed 96-well plates (VWR, Radnor, USA) and incubated in a 95% humidified atmosphere with 5% CO2 at 37 °C for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... for duck fibroblasts (i.e., CCL-141 cells) supplemented with 10% Fetal bovine serum (FBS, 97068-085, Lot# 283K18, VWR, Radnor, PA, USA) and 1X penicillin-streptomycin (15140122 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each plant was sprayed for one minute and the collected flow through from each plant were first strained using a 40 μm cell strainer (VWR 76327-098) to remove large microorganisms and large particles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903], 10mM KH2PO4 [VWR, 470302] ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Cell Biology 2022Quote: The lipidomics solubilization buffer used to resuspend whole-cell lysates and GolgiTAG immunoprecipitates was made of LC/MS grade chloroform (VWR. Cat# 22711.260) and Optima LC/MS grade methanol (Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... when confluence was reached and counting/replating using Trypan blue exclusion dye in an automatic cell counter (EveTM, NanoEnTek distributed by VWR, Milan, Italy) in accordance with the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: Total RNA of minigene-transfected HEK293T cells and patient-derived fibroblasts was extracted using the peqGOLD Total RNA Kit (VWR Life Science). One microgram of RNA was treated with 1 U of DNaseI (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... After a PBS wash 500 µl TRIzol was added to the cells followed by the addition of 300 µl chloroform in a heave gel phase-lock tube (VWR, 10847-802) for phase separation using centrifugation ...
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bioRxiv - Bioengineering 2024Quote: ... The cells collected from the BM and spleen were filtered through 70 μm mesh and enriched for haemopoietic lineage cells by density centrifugation using Lympholyte-Mammal (VWR, Lutterworth, UK), centrifuging the cell suspension for 20 min at 625 g at RT ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Neuroscience 2019Quote: ... was made with a dental drill (Osada) for injection into the target (see Table 1) using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were thawed on ice and resuspended in 250 μL of PBS supplemented with 1 M NaCl and 6 M Urea (VWR). The resuspended bacteria were then added to screw cap microcentrifuge lysing tubes (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Neuroscience 2020Quote: ... and then cryoprotected for 3 days at 4 °C in 30% sucrose solution (w/v in 1× PBS; VWR Chemicals). Brains were then embedded into optimal cutting temperature (OCT ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... The patterned PDMS chip was then plasma bonded to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the aqueous phase was tranferred to a new tube and RNA precipitated with isopropanol (0.5 mL per 1 mL of trizol, VWR, Denmark) by incubation at −20 °C for two hours followed by cenrifugation (12 000 g ...
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bioRxiv - Biophysics 2020Quote: ... the diluted protein was loaded to BSA (200 μl of 1 mg/ml for 30 seconds) treated coverslips (no. 1.5H precision cover slides, VWR Marienfeld), minimizing fluorophore interactions with the glass slide ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% Fast green (Raymond A Lamb) (both in distilled water) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Systems Biology 2022Quote: Samples were grown using SD-His+G418 agar and medium selecting for the duplicated chromosomes (6.7 g/L yeast nitrogen base without ammonium sulfate, Difco Cat#233520; 20 g/L glucose, Sigma Cat#Y0626; 1 g/L monosodium glutamate, VWR Cat#27872.298 ...
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bioRxiv - Immunology 2022Quote: Bone marrow isolation and cell staining was performed on ice in staining medium (SM; Hanks Balanced Salt Solution [HBSS 1x, Corning 1-022-CM] supplemented with heat inactivated fetal bovine serum [FBS, VWR 97068-05] to a final concentration of 2%) ...
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bioRxiv - Microbiology 2019Quote: ... excess oil was removed by pipetting and water-in-oil emulsions were broken by adding an equal amount of 1H,1H,2H,2H-Perfluoro-1-octanol (PFO, VWR) and briefly vortexed ...
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bioRxiv - Microbiology 2019Quote: ... ground with 1 mL of phosphate buffered saline using the sterile 15 mL conical tissue grinder (VWR™, Radnor, PA). Samples were spun at 5,000xg ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...