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Citations for VWR :
1701 - 1750 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...
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bioRxiv - Microbiology 2019Quote: ... devices were treated in oxygen plasma and covalently bound onto borosilicate cover glass slides (round, 50 mm diameter, thickness No. 1, VWR). All microflow experiments were conducted in single microchannels with dimensions of either of μm in width and 100 μm in height or a width of 50 μm and a width of 200 μm.
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bioRxiv - Genomics 2019Quote: ... The mix was filtered through a set of sieves (1 mm/500 μm/38 μm × 200 mm diameter, (VWR, Sweden). The content of the last sieve was transferred into a falcon tube containing 20 ml of 60% sucrose solution ...
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bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50 μL of 1% (w/v) sodium dodecyl sulfate (SDS, VWR International, Radnor, PA, USA). Colorimetric absorbance at 414 nm was measured using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Pathology 2021Quote: ... 10% glacial acetic acid) at a 20:1 ratio for 30-45 min and 10% neutral buffered formalin (VWR scientific) at a 10:1 ratio for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... ∼1 in wide and 3-4 in long into a 15 cm petri dish (CA25383-103, VWR, Edmonton, AB, Canada) and created spherical depressions ∼2.5-3 mm in diameter to create a mold for the Matrigel ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Microbiology 2022Quote: ... The in-house polyacrylamide TBE gels (40 ml) for horizontal electrophoresis were produced by combining 6 ml 40% Acryl/Bis 29:1 (VWR), 4 ml of 5x TBE ...
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bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
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bioRxiv - Molecular Biology 2023Quote: ... Tissue sections were rinsed of excess probes in 2X SSCT for 1 min and washed twice in 50% formamide (VWR) in 2X SSC at 37°C for 25 min ...
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bioRxiv - Microbiology 2023Quote: ... in PBS pads were placed on the sample and were covered with 20×20 mm No 1 cover glass slide (VWR). The open sides between two cover glass slides were sealed using VALAP solution (1:1:1 w/w Vaseline-Lanolin-Paraffin).
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bioRxiv - Plant Biology 2023Quote: ... stained with 1% w/v toluidine blue in 0.5% w/v sodium tetraborate for 5 seconds and mounted in DePeX (VWR, Belgium). For vibratome sectioning ...
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bioRxiv - Biophysics 2023Quote: ... at a mass ratio of 1000:1 TO:PL and bath sonicated for 30 min (Ultrasonic Cleaner 96043-936; VWR Symphony) to form aLDs ...
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bioRxiv - Developmental Biology 2023Quote: ... and then to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Developmental Biology 2023Quote: ... Bulk samples including bone and bone marrow were cryo-pulverized and resuspended in 1 ml ice-cold TriFast (VWR International) and carefully vortexed (30 sec) ...
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bioRxiv - Bioengineering 2023Quote: The strains were grown overnight in M9 minimal media (4 g/L glucose, 1 g/L casamino acids (cat. no 2240, VWR), and 1 mg/L thiamine (cat ...
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bioRxiv - Bioengineering 2023Quote: ... The product was purified by a dialysis bag with 1 K MWCO followed by filtration with VWR syringe filter (0.22 μm; 76479-010; VWR, PA). To load DOX to GQDs (e.g. ...
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bioRxiv - Cell Biology 2023Quote: ... The thin polyacrylamide-based substrate was polymerized between two different glass coverslips (#631-0162, diameter 32 millimeters, thickness No. 1, VWR) prepared as follows ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Microbiology 2023Quote: Cell extracts were dried in a nitrogen stream at room temperature and resuspended in either 200 µL methanol for diatom extracts or methanol/acetonitrile/water 5:9:1 (acetonitrile: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the enriched PBMCs at the middle layer were carefully removed and washed twice in 1% BSA (VWR, 9048- 45-8) in DMEM (Corning ...
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bioRxiv - Microbiology 2024Quote: ... Then it was used to seed a main culture (200 µl final volume of BMM with desired concentration of Ca2+ and/or Fe) at a ratio of 1:100 in 96 well white clear bottom plates (VWR). The plates were covered with lids treated with a mixture of 95% ethanol and 1% Triton solution to prevent condensation ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Bioengineering 2024Quote: ... Oral rinses in the anatomical scaffolds were performed on day 3 and day 5 post-inoculation using Listerine® Antiseptic mouthwash original (brown color) diluted in PBS (1x) to 1% and filtered using a vacuum filtration system (0.2 µm) (VWR, Philadelphia). Briefly ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into 200 μL fresh culture medium in one well of a 96-well flat bottom plate (VWR Catalog #29442-054).
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bioRxiv - Biophysics 2021Quote: ... the fluidic sample cells were constructed using two pieces of double-sided tape sandwiched between a microscope slide and glass coverslip (VWR 22×30 mm). Each microscope slide had a hole on each of two ends ...
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bioRxiv - Cell Biology 2020Quote: ... The cultivation media used for each cell line was recommended by the supplier with an addition of 10% Fetal Bovine Serum (FBS, VWR, Radnor, PA, USA). For immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
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bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.
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bioRxiv - Genetics 2019Quote: We performed cell culture using the conditions described above in 12-well plates containing sterile 18-mm glass cover slips (VWR, Cat#48380-046). We fixed cells for 10 minutes in 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspensions were filtered through a 35 μm nylon filter cap on 12 × 75 mm round bottom tubes (VWR/Falcon 21008-948). Flow cytometric measurements were performed using a BD LSR Fortessa ...
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bioRxiv - Genomics 2022Quote: ... HeLa S3 or HeLa S3 + TOP2A- Venus cell linescells were either grown or spun onto 22×22mm No 1.5 coverslips in 6-well plates (VWR Cat No 48366-277). (cell cycle analysis experiments ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Microbiology 2023Quote: ... Adjusted cultures were then mixed in a 2.5:97.5 ratio of fluorescent:non-fluorescent cells and nine ml of this mixture was added to 13×100mm culture tubes (VWR, Cat no. 10545-936) and grown in 25°C incubator for three days ...
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bioRxiv - Cancer Biology 2024Quote: 5×106 MM.1Sgfp+/luc+ cells were treated with DMSO or 1.00 μM TriC for 24 h in T-25 flasks (Avantor/VWR; Cat. no. 10861-568). Cells were then harvested ...
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bioRxiv - Bioengineering 2024Quote: ... PCLS were then placed on Gelfoam® for long-term culture and monitored using a WST-8 cell counting kit (VWR #89155-898) according to the kit’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All evolution was conducted at 30°C in 128μL of YPD (a rich laboratory media; 1% Bacto yeast extract (VWR #90000– 722), 2% Bacto peptone (VWR #90000–368) ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Molecular Biology 2021Quote: We prepared Aβ1-42 oligomers (oAβ) and fibrils (fAβ) following Evans (2006) and Ryan et al (2010) using siliconized polypropylene tubes (VWR #4165SL). We used Aβ1-42 to test the effects of the hybrid protein because it is more predominant in AD plaques (Nirmalraj et al. ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... Individual CNSs were sonified with five 0.3 s pulses at 1 Hz using a Branson Sonifier 450 (VWR Scientific, Winchester, PA) in Laemmli buffer (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... and the seeds were removed with forceps and put in a drop of clearing solution (1 vol glycerol / 7 vol chloral hydrate liquid solution, VWR Chemicals) between a slide and a coverslip ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...