Labshake search
Citations for Zymo Research :
6001 - 6050 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction product was treated with DNAseI and shRNAs were purified using the RNA Clean and ConcentratorTM Kit (Zymo Reasearch, R1012).
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bioRxiv - Developmental Biology 2022Quote: ... Gel band within 100-250bp was cut and purified using Zymoclean Gel DNA recovery kit/ DNA Clean & Concentrator-5 (Zymo Research). If needed ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by a 5-day incubation period before total RNA was collected by Direct-zol RNA MiniPrep Plus kit (Zymo Research). 69 RNASeq profiles ...
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bioRxiv - Biochemistry 2022Quote: ... and transformed into the EBY100 yeast surface display strain [14] that had been made chemically competent using the Frozen EZ-Yeast Transformation II Kit (Zymo Research). For each single site-directed mutant library transformed yeast were plated onto three each Synthetic Dropout minus Tryptophan (SD -Trp ...
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bioRxiv - Molecular Biology 2022Quote: ... a total of 30 μg of isolated total RNA per replicate was purified with the Quick-RNA Microprep Kit (Zymo research) and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad) ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: The heterodimer bands were cut from agarose gel (Figure 4a) and the RNA content was recovered with Zymoclean Gel RNA Recovery Kit (Zymo Research) using standard procedures ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). Transient expression of individual variants for western blots was carried out using a distinct set of expression vectors encoding the untagged CFTR variant cDNAs in the context of a pCDNA5 expression vector ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: ... RNA isolation from the lysates was performed according to the Ambion/Life Technologies protocol using Trizol reagent and RNA Clean and Concentrator kit (Zymo Research). Isolated RNA pool was hybridized with TagSet master mix (nCounter Elements™ XT Reagents ...
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bioRxiv - Cancer Biology 2022Quote: One microgram of genomic DNA was treated with sodium bisulfite using the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volume of ethanol was added to the sample and RNA is isolated using the Direct-zol kit (Zymo research, R2050). The RNA eluted from the column was treated with TURBO DNase kit (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was isolated from biological triplicates of XX and XXY ovaries (60/sample) by Direct-zol miniprep kit (Zymo Research). Reverse transcription and qPCR were performed following the manufacturer’s protocol using TaqMan MicroRNA Reverse Transcription Kit ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... yeast (PUP1/pup1Δ::kanMX) with a Magic marker selection were made using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For maximum representation of mutant human gene clones transformed in the strain ...
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bioRxiv - Genetics 2022Quote: ... Resulting cDNA was separated on a 4% agarose gel and the 160–190 nt long fragments were extracted and gel purified using the Zymoclean Gel DNA Recovery Kit (D4008, ZYMO RESEARCH). Small RNA Libraries were sequenced in triplicates on a NovaSeq S1 SR100 mode (Illumina ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from 12 infection assay samples and one pooled sample consisting of root and flower bud material (pooled from different floral stages) using a Direct Zol RNA Miniprep Plus kit (Zymo Research) followed by DNAse treatment ...
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bioRxiv - Genomics 2022Quote: ... was added to extract total RNA using the Direct-zol RNA Mini Prep Plus kit (Zymo Research, Irvine, CA, United States). The integrity and quality of extracted RNA were checked using an Experion electrophoresis system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... An amount of 200 ng of genomic DNA from each sample underwent bisulfite conversion with Zymo DNA Methylation Gold Kit (Zymo Research). The converted DNA served as a template for PCR amplification and library preparations as in the former section ...
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bioRxiv - Genetics 2022Quote: RNA was extracted from pellets of ∼2 million iPSCs or ∼6 million neurons immediately after harvesting with the Quick-RNA Miniprep Kit (Zymo R1054) following manufacturer’s instructions except for two alterations ...
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bioRxiv - Physiology 2022Quote: ... validated clones were amplified in a large scale (150 ml) and the plasmid DNA was isolated using ZymoPURE Plasmid Maxiprep kit (Zymo Research). Endotoxin was eliminated by treating the plasmid with Endozero columns (Zymo Research).
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bioRxiv - Microbiology 2022Quote: Purified AdSDV-injected house crickets were collected three days post inoculation and total RNA was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research), where the denaturation step was executed by adding TRIzol Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ~1 kb products from the Pan-Entamoeba PCR above were gel purified using a Zymoclean Gel DNA Recovery Kit (Zymo Research) and submitted to the UW-Madison Biotechnology Center for Sanger Sequencing using the amplification primers described above ...
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bioRxiv - Microbiology 2022Quote: ... at -80ºC until DNA extraction with the Zymo Quick DNA 96 Plus and ZR-96 Clean-up kits (Zymo Research, Inc.). High-throughput Illumina 150-bp paired-end libraries for all six samples were prepared and sequenced by Novogene Corporation ...
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bioRxiv - Microbiology 2022Quote: ... Beads were washed three times with 1ml PBS and RNAs were eluted with Trizol and extracted with Direct-zol RNA miniprep kit (Zymo Research) followed by RT-qPCR ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from 100mg (approximately) of flower buds using TRIzol and the Direct-zol RNA Miniprep kit (Zymo, R2050). Sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) ...
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bioRxiv - Plant Biology 2022Quote: One leaf tissue of 4- to 5-week-old plants with similar age from fwa and early flowering ZF transgenic lines were collected for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research). 1μg of total RNA was used to prepare the libraries for RNA-seq following TruSeq Stranded mRNA kit (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: Viral RNA in supernatants of infected cells and intracellular RNA were extracted using Quick-RNA™ Viral 96 Kit (Zymo Research). RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from primary neurons was extracted using home-made TRIzol reagent in combination with Direct-zol RNA Kit (Zymo Research). The samples were fixed in cold methanol for 10 mins at −20C ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from larval fat bodies (2-5 pooled/sample) or larval brains (5 pooled/sample) using the Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Plant Biology 2023Quote: Rossette leaves of about one-month-old plants were collected for RNA extraction with Zymo Direct-Zol RNA miniprep Kit (Zymo Research). 1 ug of RNA were used for cDNA synthesis with iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... 500 ng of genomic DNA from each sample was bisulfite-converted with the EZ DNA Methylation™ Kit (Zymo Research, D5002) and analyzed using Infinium HumanMethylationEPICBeadChip™ Kit (Illumina ...
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bioRxiv - Genetics 2022Quote: ... Leukocyte genomic DNA (500 ng) was treated with bisulfite using the DNA EZ Methylation-Gold methylation kit (Zymo Research, CA, USA), following the recommendations stipulated by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: RNAlater-preserved samples were homogenized in PBS and total RNA was extracted from samples using the Quick-RNA Miniprep Kit (Zymo Research). RNA extracts were evaluated for concentration and purity using a Nanodrop 8000 instrument (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... At the indicated time points we harvested cells and extracted RNA using the Direct-zol-96 RNA kit (Zymo #11-331H). Samples were then sent to Novogene for bulk RNA-seq.
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neocortical regions of slice cultures using Trizol reagent followed by RNA Clean & Concentrator kit (Zymo research R1014). cDNA was synthesized with 0.5 μg of RNA using the cDNA Synthesis Kit (Bio-Rad 170-8891 ...
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bioRxiv - Systems Biology 2023Quote: ... to exclude small fragments (less than about 200bp) and the DNA was eluted from the gel slices using the Zymoclean Gel DNA Recovery Kit (Zymo, #D4001), followed by quantification (Qubit ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
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bioRxiv - Immunology 2023Quote: ... DNA obtained from anti-OVA hits were then cut out from agarose gels and amplicons from heavy and light chains were purified separately by gel extraction (Zymoclean Gel DNA Recovery Kit, Zymo Research).
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bioRxiv - Genomics 2023Quote: ... Tissue was then homogenized with steel beads and total RNA was extracted using the Direct-zol™ RNA MicroPrep kit from Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... The genomic DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit following the protocol of the manufacturer (Zymo Research). DNA was amplified by 30 cycles of a three-step PCR (98℃ for 10 sec ...
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bioRxiv - Evolutionary Biology 2022Quote: RNA was extracted from 30 μL of each virus inoculum using a Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... was added to 200 μl of homogenized tissue (see DNA extraction) and processed using the Direct-zol RNA Miniprep Kit (Zymo Research) with on-column DnaseI digestion ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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bioRxiv - Molecular Biology 2023Quote: RNA for RNA-seq was obtained by processing cerebellar tissue or CGN cultures with Trizol reagent followed by clean up on the Zymo Direct-zol RNA miniprep kit (Zymo #R2052). RNA purity was measured to ensure samples had A260/280 and A260/230 values > 1.9 ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...