Labshake search
Citations for Zymo Research :
5901 - 5950 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... using an in-house Tn5 transposase (IMBA-IMP-GMI Molecular Biology Services) and an EZ-96 DNA Methylation-Gold Mag Prep kit (Zymo Research) for bisulfite conversion ...
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bioRxiv - Plant Biology 2023Quote: ... microdissected material of around 80 gynoecia (sections of around 24 paraffin blocks with 3 or 4 embedded inflorescences) was pooled in a single tube and RNA extraction was performed with Direct-zol RNA MicroPrep kit (Zymo Research). The integrity and concentration of extracted RNA of each sample was measured using a NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from frozen tissues using Direct-zol RNA Miniprep plus kit following the manufacturer’s protocol (Zymo Research - R2073). The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were then size selected by gel purifying DNA on a 10% TBE gel and cleanup was performed using Zymo DNA Cleanup kit (Zymo # D4014) and the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Total RNA was isolated from the received fungal pellet using conventional TRIzol method followed by column purification using Quick RNA Plant MiniPrep Kit (Zymo Research). The qualities and quantities of the isolated RNA were checked on NanoDrop followed by Agilent TapeStation using High Sensitivity RNA ScreenTape.
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bioRxiv - Molecular Biology 2023Quote: ... RNA from each fraction was first precipitated in EtOH/C2H3NaO2 and then purified through RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Reaserach, R1016). RNA distribution profiles were analyzed by qRT-PCR.
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated using the trizol method as described by the manufacturer’s protocol using the Directzol RNA isolation kit (Zymo Research, Germany). The RNA concentration was determined by UV measurement ...
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bioRxiv - Genomics 2023Quote: ... All colonies from each reaction were scraped from the plate and the CROP-seq-opti-Puro-T2A-GFP-sgRNA plasmid library was extracted using ZymoPURE II Plasmid Midiprep Kit (Zymo, D4200). The lentiviral library was generated as stated above with extended virus production time ...
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bioRxiv - Microbiology 2023Quote: ... DNA and RNA were coextracted from filters at Colorado State University using ZymoBIOMICS DNA/RNA Miniprep Kit (Zymo Research Cat. # R2002) coupled with RNA Clean & Concentrator-5 (Zymo Research Cat ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from homogenized colon tissue (fresh, stored in RNAlater or Trizol) or isolated crypts using the Quick-RNA Miniprep Kit (R1055, Zymo Research), or for samples in Trizol ...
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bioRxiv - Microbiology 2024Quote: ... DNA products were excised around the 400 bp size range and purified using the Zymo Gel DNA Recovery kit (Zymo Research). Libraries were quantified by Qubit ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from the heads of 25-30 adult male flies using TRIzol Reagent and Direct-zol RNA microprep kit (Zymo Research) followed by cDNA preparation from 0.5–1 μg total RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was purified from all input and output library cultures using the Quick-DNA™ Miniprep Plus Kit (Zymo Research), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were then size selected to remove adaptor-dimers below 200 bp using three subsequent size-selection rounds with a Select-a-Size DNA Clean & Concentrator Kit (D4080, Zymo Research). Finally ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL each reaction was transformed into 100 μL DH10B and transformed according to the Mix and Go Transformation kit (Zymo Research).
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bioRxiv - Plant Biology 2022Quote: ... genomic DNA was extracted from leaf tissue of ASF1B-ZF T2 with a CTAB-based method and converted using the EZ DNA Methylation-Lighting kit (ZYMO research). We amplified the methylated region of the FWA promoter region and several control regions with primers described previously48 ...
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bioRxiv - Neuroscience 2022Quote: ... Bisulphite conversion was then carried out with 750 ng of DNA using the EZ DNA Methylation Kit (Zymo Research, Irvine, USA), followed by genome-wide methylation profiling using the Infinium HumanMethylationEPIC Bead Chip (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: The cells were lysed in TRIzol (Thermo Fischer Scientific) and the total RNA was extracted with Direct-zol RNA extraction kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA for long read sequencing was extracted from our Salmonella enterica serovar Typhimurium strain 14028 isolate using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Library preparation was performed using the Genomic DNA by Ligation (SQK-LSK 109 ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from rosette tissue harvested at ZT 23 on day 25 post imbibition (n = 4 biological replicates) using the Direct-zol-96 MagBead RNA kit (Zymo Research). RNA was checked for degradation using a Tapestation 4150 (Agilent ...
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bioRxiv - Physiology 2024Quote: ... were manually dissected out from the slices using 27 G needles and placed immediately into RNA lysis buffer from the Quick-RNA Microprep Kit (Zymo, R1050). Total RNA was isolated from tissue using the Quick-RNA Microprep Kit (Zymo ...
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bioRxiv - Bioengineering 2024Quote: Up to 1 × 108 yeast cells from a growing culture were taken to perform a zymoprep using the ZymoprepII kit (D2004, Zymo Research). The TRACeR sequences were amplified from the extracted plasmids and the adapter sequences were added by PCR ...
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bioRxiv - Bioengineering 2023Quote: ... and RFs with a size greater than 17nt was isolated according to the RNA Clean and Concentrator-25 kit (Zymo Research). After obtaining ribosome footprints ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with 1.25 units of RQ1 DNase for 30 minutes at 37°C and RNA recovered using an RNA Clean & Concentrator-25 spin column kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and the total RNA of each sample was isolated with the Direct-zolTM RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... To determine the amount of virus that bound the cells viral RNA was isolated with RxnDirect-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2024Quote: The infected cells or homogenized lungs were lysed in Trizol to prepare total RNAs using Direct-Zol RNA Microprep kit (Zymo Research) with DNase treatment according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from samples collected at the 48h timepoint (72h after the transfection) using Quick-DNA/RNATM Microprep Plus Kit (Zymo Research) including the DNAse I step ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using protocols from the Quick-RNA Fecal/Soil Microbe Microprep Kit with some modifications (Zymo, Irvine, California, USA). RNA samples were initially preserved with 1 mL of activated sludge (i.e. ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from corals at the 48h timepoint (72h after the transfection) using Quick-DNA/RNATM Microprep Plus Kit (Zymo Research). The level of 8-Hydroxy-2’-deoxyguanosine (8-OhdG ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in 50-100 mL of LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE II Plasmid Midiprep Kit (Zymo #D4201) by following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: For short read Illumina sequencing DNA extractions were carried out with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, UK), with the addition for S ...
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bioRxiv - Molecular Biology 2024Quote: ... The bacmid encoding Halo-Ku80/Ku70 complex was obtained by electroporating pAM086 into DH10EMBacY electro-competent cells and purified using ZR BAC DNA miniprep kit (Zymo Research).
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bioRxiv - Molecular Biology 2024Quote: RNA purification and on-column DNase I treatment were performed using a Quick-RNA Fungal/Bacterial Miniprep kit and DNase I set (Zymo Research) following the manufacturer’s recommendations with minor modifications ...
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bioRxiv - Genomics 2024Quote: ... Purified genomic DNA from undifferentiated and differentiated adipocytes was subjected to bisulfite modification using the EZ DNA Methylation kit (Zymo Research). Approximately 0.5-1μg of genomic DNA was treated with bisulfite and eluted in 20μl elution buffer following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was purified from bilateral NAc core and shell separately using the Direct-zol RNA kit following the manufacturer’s instructions (Zymo Research, CA), including DNase to remove genomic DNA ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA from 105 days old CorticOs (N = 4; n = 6-8 per genotype) was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... The total microbe DNA from 100 mg of bulk or rhizosphere samples was extracted using the Quick-DNATM Fecal/Soil Microbe Miniprep kit (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we resuspended the pellet in 200 µL of nuclease-free water followed by addition of 750 µL of BashingBead buffer from the Direct-Zol RNA MicroPrep RNA kit (Zymo Research). We then transferred our 950 µL of suspension into BashingBead tubes ...
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bioRxiv - Genomics 2024Quote: ... secondary plant compounds hampering the activity of the pores in the Oxford Nanopore Technologies (ONT) flow cells were removed with the Quick-DNA HMW MagBead Kit (Zymo Research). Sequencing libraries were prepared with the LSK-109 ligation library preparation kit and sequencing was performed on MinION R.9.4.1 flow cells ...
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bioRxiv - Genomics 2024Quote: ... The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB) + 50 μg/mL Ampicillin and purified using the ZymoPURE II Plasmid Midiprep kit (Zymo D4200) according to manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: cfDNA was isolated from 5 ml plasma or 5 ml ascites using the Quick-cfDNA Serum & Plasma Kit centrifugation protocol (Zymo Research) for HC ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... DNA was eluted in 105 µL RNAse free H2O and was further cleaned using OneStep PCR Inhibitor Removal Kit (Zymo Research). Concentrations for all DNA samples were measured using Qubit 3.0 Fluorometer (Thermo Fisher Scientific ...