Labshake search
Citations for Zymo Research :
5951 - 6000 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Genomic DNA was isolated from previously frozen cell pellets and bisulfite converted using the EZ DNA methylation-direct kit (Zymo Research). Two PCRs were performed with the PyroMark PCR kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... we extracted total RNA from each line (5 wt lines and 5 +0A lines) using the Zymo Direct-Zol RNA extraction kit (Zymo Research). This RNA was immediately reverse transcribed using Superscript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: Isolates for sequencing were cherrypicked using an Opentrons OT-2 Liquid Handler and DNA extraction was then performed using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research) on a KingFisher Flex instrument (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The vector and PCR products were digested with restriction enzymes and band-purified (vector) or cleaned (PCR products) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) or the DNA Clean & Concentrator-5 kit (Zymo Research) ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary validation of genotyping reactions was performed as needed using the Quick-DNA Plant/Seed Miniprep kit (Zymo Research, cat#D6020). Bulk Illumina and Nanopore data from Tpd1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The soluble fraction was mixed with equal volume of 100% ethanol and RNA was isolated using the Direct-zol RNA Miniprep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... tubes were centrifuged at 12,000 × g for 15 min at 4°C and aqueous phase were transferred into a new tube and RNA was extracted with the RNA Clean and Concentrator kit (ZYMO Research).
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 1% Agarose gel and the band at the appropriate size was excised and the DNA extracted using the Zymoclean Gel DNA Recovery kit (Zymo Research). Purified insert DNA was cloned into the linearized modRNAc1 plasmid using the In-Fusion Cloning Kit (Takara Bio) ...
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bioRxiv - Microbiology 2023Quote: Isolated poly(A)+ RNA was firstly subjected to a clean-up and concentration step using the RNA clean & concentrator kit (Zymo Research). Then ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 200-250µm-thick cryopreserved rat lung tissue using the Zymo Quick-DNA MicroPrep Kit (Zymo, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Approximately 50 ng genomic double-stranded DNA per donor was converted with sodium bisulfite using the EZ DNA Methylation-Lightning Kit (Zymo Research) and fragmented by sonication (Covaris S220 ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from frozen tissues using Direct-zol RNA Miniprep plus kit following the manufacturer’s protocol (Zymo Research - R2073). The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA ...
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bioRxiv - Plant Biology 2023Quote: ... The aqueous phase was extracted and cleaned up using the Zymo RNA Clean and Concentrator-5 kit (Zymo Research, cat#R1013). Only samples with RNA integrity (RIN ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tissues were disrupted in Trizol using a Premium Multi-Gen 7XL Homogenizer (PRO Scientific) and RNA was subsequently extracted using the Direct-zol RNA kit (Zymo Research), including a DNAse step to remove genomic DNA contamination ...
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bioRxiv - Genetics 2023Quote: ... Five hundred nanograms of gDNA was bisulfite converted following manufacturer’s specifications using the EZ DNA Methylations kit (Zymo Research; Irvine, CA). Bisulfite-treated gDNA was amplified from −660 to +97 from the transcription start site of Pl1-Rhoades (Cone et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 88∼135 bp) were resolved on a 10% TBE polyacrylamide gel and extracted separately using the DNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from temporal cerebral cortex samples using the Zymo Quick-DNA/RNA Miniprep Plus Kit (Zymo, Irvine, CA, USA) and purified using the RNA Clean and Concentrator-5 kit (Zymo) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Synthetic Biology 2024Quote: ... colonies were scraped with 50 mL 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203). These plasmid libraries constitute the “post-selection libraries.”
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bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE Plasmid Miniprep Kit (Zymo #D4210) by following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 50 mg of finely minced tissue in all cases using the Quick-RNA™ Plant Miniprep kit from ZYMO RESEARCH® (ref ...
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bioRxiv - Neuroscience 2023Quote: ... and RNA was extracted with the phenol-chloroform-based procedure or using Direct-zol RNA Miniprep Plus kit (Zymo research, R2070) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants for each sample were carefully transferred to RNase-free tubes for purification by the Direct-Zol RNA Miniprep Plus RNA purification kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RNA was purified and concentrated according to the protocol for RNA Clean and Concentrator Kit - 5 (Zymo Research Corporation, Irvine, CA) with an eluted volume between 30-35µL ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cultured cells or myocardial tissue of mice euthanized at the timepoints reported above using the Direct-zol RNA purification Kit (ZYMO Research) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... nucleus accumbens and lung of the 4 juvenile and 4 old female mice was bisulfite converted using the EZ DNA methylation Gold kit (Cat# D5006, Zymo Research) according to manufacturer’s instructions and eluted ...
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bioRxiv - Biochemistry 2023Quote: ... All plasmids used for transient transfection experiments were purified with an endotoxin removal step (ZymoPURE Plasmid Miniprep Kit from Zymo Research). The PEmax** protein expression vector (pET-21a-PEmax**-6His ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 2% Agarose gel and the band at 150 bp was excised and then purified using the Zymoclean Gel DNA Recovery kit (Zymo Research). The dgRNA was synthesized via IVT using the MEGAscript T7 Transcription kit supplemented with 10mM ATP ...
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bioRxiv - Genomics 2023Quote: ... was transformed with 2 uL of each ligation reaction and resulting colonies were selected for plasmid DNA isolation using the ZymoPure Plasmid miniprep kit (Zymo Research). Each cloned gRNA sequence-containing PB-CRISPRia plasmid was verified by Sanger sequencing (Quintara Bio).
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Microbiology 2023Quote: ... the same PCR fragment used for Sanger sequencing was purified using a ZymocleanTM Gel DNA Recovery Kit (Zymo Research, cat# D4002). DNA (∼1 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... gel fragments corresponding to 190 – 350 nts in DNA size were excised and libraries extracted using the Zymoclean Gel DNA Recovery Kit (D4007, Zymo Research). DNA concentrations were measured using the Qubit dsDNA HS Assay Kit (Q32854 ...
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bioRxiv - Plant Biology 2023Quote: ... and was size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research, Irvine, CA. USA). The final library was sequenced using a 2 × 150 bp paired-end protocol on the NovaSeq 6000 system (Illumina).
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bioRxiv - Microbiology 2023Quote: ... were grown at 28°C to stationary phase and cell pellets from 5 mL cultures were collected for total RNA extraction using a Direct-zol RNA miniprep kit (Zymo Research). 10 μg of isolated total RNA was subjected to DNaseI treatment with 20 units of Turbo DNaseI (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and PPL554 of bacterial cultures grown overnight in NB media overnight was extracted using the QuickDNA™ Fungal/Bacterial Miniprep Kit sourced from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: Stored bacterial pellets were used for RNA extraction using the Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... were transformed into Saccharomyces cerevisiae VL6-48(Yamanaka et al., 2014) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). The correct promoter insertion was screened by PCR using primers upstream and downstream of the target site (Table S4) ...
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bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
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bioRxiv - Plant Biology 2024Quote: ... was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Plant Biology 2024Quote: ... A 200 mg soil sample from each falcon tube was taken for DNA extraction using the Zymo Quick DNA Fecal/Soil Microbe MiniPrep Kit (Zymo Research). Before sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... which were removed from the mutational libraries by gel purification using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Purified products were again transformed into either NEB 10-beta (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... The total RNA of isolated renal tubules was immediately extracted using Quick-RNA™ MicroPrep kit (Zymo Research, Irvine, CA, USA). Reverse transcription was performed using Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: We used a Trizol-based protocol to extract RNA from primate samples using the Direct-zol RNA Microprep kit (R2060, Zymo Research). All steps were centrifuged at 13,000 g for 30 s unless otherwise noted ...
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Molecular Biology 2023Quote: ... the non-poly(A) RNA segments in the flow-through were purified using the RNA Clean & Concentrator-5 kit (Zymo Research). The resulting purified poly(A ...