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Citations for Zymo Research :
5801 - 5850 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Samples were then de-crosslinked by incubation at 94C for 20 mins and subsequently lysed and extracted for genomic DNA (gDNA) using the Quick-DNA/RNA Miniprep Kit (Zymo D7005T). gDNA samples of both samples were sent to Cellecta Inc ...
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Developmental Biology 2022Quote: ... Glycerol stocks of the plasmids that were confirmed correct by Sanger sequencing were grown and prepared for maxiprep plasmid isolation (Zyppy Plasmid Maxiprep Kit, Zymo 6431). Proper cloning was achieved for all gRNAs.
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bioRxiv - Evolutionary Biology 2022Quote: ... The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina was used for library preparation and was combined with EZ-96 DNA Methylation-Gold™ MagPrep (ZYMO) for bisulfite libraries ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from the brain and muscle tissue separately using a Quick-DNA™ MagBead Plus kit (Zymo Research, 2017) and quantified using a Qubit™ dsDNA HS Assay Kit on a Qubit™ 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and the total RNA was then extracted using the Direct-zol RNA MiniPrep Kit (R2052, Zymo Research) and quantified using NanoDrop 2000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was first extracted from pools (10 flies for each cage and time point) using the Quick-DNA Plus kit (Zymo D4068), which includes a proteinase K digestion to ensure unbiased sampling of diverse bacteria ...
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bioRxiv - Plant Biology 2022Quote: ... YES3-Gate-OPT3 construct or YES3-Gate lacking the cDNA insert were transformed into appropriate yeast line using the Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformants were selected for uracil prototrophy on YNB medium containing 6.7% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then placed on a magnet and the immunoprecipitated DNA samples in the supernatant along with the input samples were purified using ChIP Purification Kit (Zymo Research), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified from proteinase K treated samples using a DNA purification kit following the manufacturer instructions (Zymo Research, CA, USA) and was subsequently analyzed either by running a 2% low melting agarose (APEX ...
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bioRxiv - Microbiology 2022Quote: ... DNA and RNA were extracted using the Quick DNA/RNA mini prep plus kit with ZR BashingBeads (0.1 and 0.5mm; Zymo Research, Carlsbad, CA). For coral samples ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: ... gastrocnemius muscle RNA from Sham and SMC mice were isolated with the Direct-zol RNA Miniprep Plus kit (Zymo Cat#: R2070). RNA library construction and sequencing were performed by the High-Throughput Genomics Core at the Huntsman Cancer Institute ...
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bioRxiv - Developmental Biology 2020Quote: ... and Spag17Pcdo/Pcdo animals were purified and concentrated using the Zymo DNA clean and Concentrator kit (Zymo Research Corporation, Irvine, CA). 100ng of concentrated DNA were used in each Sanger sequencing reaction.
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bioRxiv - Molecular Biology 2020Quote: ... 800 ng of DNA from each participant was bisulphite treated using the EZ Zymo Methylation Kit (D5002, Zymo Research, CA, USA) following the alternative over-night incubation conditions for use prior to the Illumina® Infinium MethylationEPIC Kit provided in the published protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The gel slice corresponding to 70-90 nucleotides was excised from the PAGE gel and the RNA fragments were recovered using ZR small-RNA PAGE Recovery Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Amplicons were excised from a 2 % agarose gel (200-800 bp fragments were excised) and purified using the Gel DNA recovery kit (Zymo Research). Library concentrations were measured with qPCR according to Illumina’s Sequencing Library qPCR Quantification Guide ...
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bioRxiv - Pathology 2020Quote: ... the cells were washed with PBS three times and RNA was extracted using an RNA extraction kit (Zymo, Irvine, CA, USA). In a parallel experiment ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA from three-week-old or four-week-old plant leaves was isolated from Arabidopsis plants and from in vitro potato cultures using a Direct-zol RNA MiniPrep Kit (Zymo Research). A TRIzol™ reagent (Invitrogen)-based protocol was used for the reverse transcription reaction performed with Superscript™ III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... RNA extractions were carried out using a modified version of the Quick-RNA™ MiniPrep kit (Zymo Research, Irvine, CA, USA), as follows ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA extracted from individual particle-attached communities was quantified through qPCR of the 16S rRNA gene using the Femto Bacterial DNA Quantification Kit (Zymo Research), which has a lower limit of detection of 20fg ...
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bioRxiv - Synthetic Biology 2020Quote: ... Mycelium was disrupted with a FastPrep FP120 system (Qbiogene) and total RNA was isolated using the Direct-zol RNA MiniPrep Kit (Zymo Research). For cDNA synthesis ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted from the tissue powder using a Direct-Zol RNA MiniPrep kit with DNase I treatment (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Genomics 2021Quote: ... The PCR products were run on an UltraPure Agarose (Thermo Fischer Scientific) gel and cDNA fragments larger than 500 nt were purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo Research). The library was prepared using the ONT 1D kit (SQK-LSK108 ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA were prepared from rosettes of three biological replicates of four (Fig. 10) and five (Fig. 6) week-old plants using the Direct-zolTM RNA Mini Prep Kit (Zymo Research). DNA contaminations were removed using Turbo DNase I ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was isolated from 10 million yeast from each round using a Zymoprep Yeast Miniprep Kit (Zymo Research; Irvine, CA). Amplicons were generated to capture the peptide through the 3C protease site ...
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bioRxiv - Immunology 2020Quote: ... Cloning and sequencing of the full ORF cDNA of AgPGE2R was performed from total RNA isolated from hemocytes perfused from non-blood fed female mosquitoes (n=50) using Direct-zol RNA Miniprep kit (Zymo research). After DNase I treatment following manufacture’s protocol (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... The remaining cells were lysed with Zymo QuickRNA lysis buffer and isolated with the Zymo QuickRNA micro kit (Zymo, Cat #R1050) using the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... RNA was isolated using two rounds of chloroform extraction followed by isopropanol precipitation or using the Direct-zol RNA Miniprep Plus kit (Zymo Research).
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bioRxiv - Genetics 2020Quote: ... An aliquot of the lysate was used to quantify total RNA concentration using the Direct-zol RNA kit (R2051; Zymo, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Products were loaded on a 2 % agarose gel and the appropriate bands were excised and cleaned up with the Zymoclean Gel DNA Recovery Kit (Zymo Research). All purified amplicons were shipped to Macrogen Europe B.V ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from cultured NSC-34 cells or primary motor neurons was isolated using DirectZole RNA Purification Kit (Zymo Research, R2052), and reverse transcribed using qScript cDNA Synthesis Kit (Quanta Biosciences ...
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bioRxiv - Physiology 2021Quote: ... bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S NGS Library Preparation Kit (Zymo Research, Irvine, CA). The bacterial 16S primers ...
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bioRxiv - Immunology 2020Quote: ... and Direct-zol RNA Miniprep Plus kits followed by on-column DNase I treatment for the removal of contaminating DNA (Zymo Research). To measure PIV5-W3 transcription ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from late third instar fat bodies (n = 4-6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Molecular Biology 2021Quote: ... we recommend the use of an appropriate extraction method for genomic DNA and subsequent bisulfite conversion using the EZ DNA Methylation-Gold Kit (Zymo Research). For samples with cell numbers <1.0 x 106 ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extraction product was diluted 20-fold in DNase/RNase free water (from Zymo Research Direct-zol™ RNA MiniPrep Kit) before recording the UV absorbance spectra on a Hewlett Packard 8452 Diode Array Spectrophotometer ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA used for V4 sequencing was extracted from each sample using the Zymo Quick-DNA Fecal/Soil Microbe 96 Kit (Zymo D6011) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-5 and LPI-15 were collected from three greenhouse-grown WT plants (∼5 ft in height) for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research) with Plant RNA Purification Reagent (Invitrogen) ...
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bioRxiv - Pathology 2021Quote: Total viral RNA was isolated from serum using a Zymo Research Corporation Quick-RNATM Viral Kit (Zymo Research, Tustin, CA; #R1040) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... 100 uL of supernatant was collected and RNA was extracted using a Quick-RNA Viral Kit as per manufacturer’ instructions (Zymo Research) for analysis by RT-qPCR.
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bioRxiv - Developmental Biology 2021Quote: ... Sheared DNA was ligated with methylated adaptors (MGIEasy WGBS Adapters-16, MGI) and subjected to bisulfite conversion using the EZ DNA Methylation-gold kit (Zymo Research). Bisulfite- converted DNA was amplified with 13 PCR cycles and purified by AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genetics 2020Quote: ... and PCR amplification protocol described above were applied to a larger set of varieties. PCR products (Fig. S1) were purified using the Zymoclean DNA recovery kit (Zymo Research) and submitted (without cloning into plasmids ...