Labshake search
Citations for Zymo Research :
6101 - 6150 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The isolated yeast populations obtained after round 4 were subjected to colony screening and plasmid recovery using a Zymoprep yeast plasmid kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated using Bio-Tri reagent (Biolab; 9010233100) and cleaned using the Zymo Direct-zol RNA MiniPrep kit (Zymo; R2050) as described by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Genomic DNA was extracted from the hippocampus using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, Irvine, CA, United States) and checked for purity and quantity using a NanoDrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from these samples as previously described (19) using a ZymoBiomics DNA Miniprep kit (Zymo Research, Irvine, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The digested reaction mix was then purified prior to Gibson Assembly using a Zymo DNA clean and concentrator kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Immunology 2024Quote: ... Samples were clarified by centrifugation at ∼21,000 x g and supernatant RNA was isolated by Quick-RNA Viral Kit (Zymo Research #R1034). RNA was quantified by NanoDrop™ 2000c (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... 0.15-0.25 grams of stool samples were extracted using the Quick-DNA Fecal/Soil Microbe kit (Zymo Research, Irvine, CA, USA). Negative extraction controls were included to ensure that no exogenous DNA contaminated the samples ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmids were prepared from 25-50 mL overnight cultures grown at 37°C in LB with appropriate antibiotics and purified using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research). Plasmids with methylated HIP1 sites were miniprepped from E ...
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bioRxiv - Synthetic Biology 2024Quote: ... gDNA was extracted from spores collected from colonies on solid plate or mycelium from bioreactor experiments using the Quick-DNA Fungal/Bacterial Miniprep Kit (ZYMO RESEARCH). For Southern-Blot ...
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bioRxiv - Synthetic Biology 2024Quote: ... Multiple PCR reactions of each fragment were pooled and purified by gel purification using the ZymocleanTM Gel DNA Recovery Kit (ZYMO RESEARCH) and used for transformation.
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bioRxiv - Biochemistry 2024Quote: ... purified DNA was subjected to bisulfite modification by means of the EZ DNA Methylation-GoldTM Kit (Zymo Research, Orange, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: Genomic DNA from placental tissues was isolated using a Quick-DNA mini prep plus kit according to the manufacturer’s instructions (Zymo Research D4068). LINE-1 methylation levels were quantified using an ELISA-based Global DNA Methylation Assay LINE-1 kit ...
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bioRxiv - Genomics 2024Quote: ... one additional library per sample was constructed using the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, Irvine, CA, USA). Each library type was pooled and 150 bp paired-end reads generated on an Illumina NextSeq 550 instrument at the University of Saskatchewan Next-Generation Sequencing Facility ...
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bioRxiv - Biophysics 2024Quote: ... DNase I digestion was stopped by adding EDTA to 15 mM (final) and RNA was extracted with RNA Clean and Concentrator Kit (Zymo Research). Eluted RNA was combined with Qubit HS RNA Reagent and Qubit HS RNA Buffer and measured using a Qubit fluorometer using the HS RNA protocol after RNA standardization (Life Technologies) ...
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bioRxiv - Genetics 2024Quote: ... cerevisiae transformants were plated on YNB plates lacking uracil and plasmid DNA was recovered from yeast transformant cells using the Zymoprep Yeast Plasmid Miniprep II Kit (D2004, Zymo Research), then transformed into DH5α Escherichia coli cells by electroporation ...
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bioRxiv - Cancer Biology 2024Quote: ... was incubated for 30 minutes on a rotating orbital.DNA recovered from dynabeads was purified with a DNA clean and Concentrator-5 kit (Zymo Research, D4013). Amplification of the recovered DNA was carried out using a whole genome amplification kit (WGA2 ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA from one full-scale lentiviral infection replicate was extracted using Quick-DNA Midiprep Plus kit and protocol (Zymo, D4075). An Alt-R S.p ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA (250 to 500 ng) of the FD and FT cells was converted by sodium bisulfite using the EZ DNA Methylation-Lightning kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA extraction from drug-resistant cells was performed using the Zymo QuickDNA™ Midiprep Plus Kit (Zymo Research, Cat. D4068), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Oligonucleotides were 5ʹ-end labeled with T4 polynucleotide kinase and [γ32P]-ATP and then purified using the Oligo Clean and Concentrator Kit (Zymo Research). Recombinant protein and oligonucleotides were incubated in 20 µl of EMSA buffer (50 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... they were washed twice in ice-cold DPBS and then collected in Tri-reagent for storage at -80°C until the bulk RNA was extracted using Direct-Zol RNA Miniprep Kit (Cat#R2050, Zymo Research).
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from HG-cultured β-cell lines and processed using the EZ-DNA Methylation Direct Kit (Zymo Research). The treated DNA was amplified by PCR and purified with a gel extraction kit (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... complete medium was transformed with the yeast-display plasmid pCTCON2 (24) using the Frozen E-Z Yeast Transformation II kit (Zymo Research) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... E.coli stocks were expanded in LB with ampicillin overnight and plasmids were isolated with ZymoPURE II Plasmid kit (Zymo Research #D4203). Lentiviral particles were prepared with Lenti X 293T cell using VSV-G pseudotyped lentiviral packaging (TakaraBio ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of DNA from the validation cohort of 20 patients (20 adenoma and 20 nontumor tissue samples) was processed with the EZ DNA Methylation-Gold Kit following the standard protocol (Zymo Research). Bisulfite conversion was followed by amplification of the target CpGs ...
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bioRxiv - Developmental Biology 2024Quote: We isolated genomic DNA from all samples using the Zymo Quick DNA mini-prep Plus Kit (Zymo Research; Cat. no. D4068). The samples were amplified and prepared for sequencing as described previously (Gilbert et al ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from 1 × 106 A549 cells treated with 0.5 % (v/v) DMSO or 5 µM FICD inhibitors (C22 or C73) for approximately 24 hours using an RNA miniprep kit (Zymo Research) and quantified using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: ... recovered for 1 hour at 30°C with shaking and then grown for 12-16 hours at 30°C prior to plasmid preparation using ZymoPURE II kits (Zymo Research). To generate the final homology-directed repair template (HDRT ...
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bioRxiv - Genetics 2024Quote: ... Sequences were confirmed by whole plasmid next-generation sequencing (Plasmidsaurus) before large-scale plasmid preparations were made using ZymoPURE II Kits (Zymo Research). The plasmids were eluted in H2O and endotoxin removal (Zymo ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 100 mg of ground tissue following the protocol of the Quick-RNA Miniprep kit (Zymo Research). The integrity of the RNA samples was assessed using the RNA Integrity Number (RIN) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from 12-day old gametophytes using Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA). Primers were designed using SnapGene with elongation factor 1 (Ceric.09G081400 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cells were harvested via centrifugation and plasmids were purified using the ZymoPure II Plasmid Maxiprep Kit (Zymo Research cat. no. D4203) with a maximum of 75 ml culture per column and eluted in water heated to 50C prior to elution ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg frozen (maximum 1 month old) lysates were used for 2C RNA preparation in each Zymo-Spin IIICG column (Quick-RNA MiniPrep kit, Zymo #R1055). The published protocol was followed for the rest of the assay ...
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bioRxiv - Plant Biology 2020Quote: ... and then both ribosome-bound RNA and denatured total RNA samples were purified with RNA Clean & concentrator-25 kit (Zymo Research, USA). RPFs were size-fractionated on 15% urea polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed in TRIzol buffer and RNA was isolated using RNA Clean Concentrate Kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... Per sample 500 ng of genomic DNA were bisulfite converted using the EZ-96 DNA methylation Gold Kit (Zymo research, Irvine, USA) according to the kit’s manual ...
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We ran the libraries out on a 1.5% agarose gel and extracted 300-800 bp fragments using a Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, USA). Then ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from Buffy coat fractions and subsequently underwent bisulfite conversion using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Methylation was then assayed using the Infinium HumanMethylation450 BeadChip (Illumina Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted from each supernatant with the Zymo Quick-DNA/RNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... run on a 2% gel as before and the 150-210bp size range was excised and purified using Zymoclean Gel DNA recovery kit (Zymo Research D4007). DNA concentration was determined by qubit using the dsDNA Broad Range assay and prepared for sequencing on an Illumina HiSeq2000 machine using a single end 100bp protocol.
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from EV isolation supernatants (i.e. EV-depleted media) was extracted using Zymo ZR urine RNA isolation kit (Zymo Research, Irvine, CA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: RNA and DNA were extracted from the same frozen cell pellets using the ZR-Duet DNA/RNA MiniPrep kit (D7001, Zymo, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... those in the correct size range were extracted and purified using a Zymoclean gel DNA recovery kit (Zymo Research, Irvine, CA, USA) and Sanger sequenced (Quintara Biosciences ...
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bioRxiv - Genomics 2020Quote: ... 10 ng of the sonicated and size selected DNA was bisulfite converted using the EZ DNA Methylation-Lightning Kit (Zymo Research, D5031) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The final NET-seq library running at ∼150 bp was extracted and further purified using the ZymoClean Gel DNA recovery kit (Zymo Research D4007). Sample purity and concentration was assessed in a 2200 TapeStation and further deep sequenced in a HiSeq 2500 Illumina Platform.