Labshake search
Citations for Zymo Research :
6251 - 6300 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... The gel was cut to purify fragments from 200 bp to 600 bp using Zymoclean Gel DNA Recovery Kit (Zymo Research, D4007). The purified DNA libraries were measured by the Qubit HS ds DNA Assay Kit (ThermoFisher ...
-
bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
-
Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... small RNAs ranging from 17-to-25 nt were excised and recovered using the ZR small-RNA PAGE Recovery Kit (Zymo Research, #R1070). TraPR ...
-
bioRxiv - Neuroscience 2024Quote: ... A total of 750 ng of DNA was subjected to bisulfite conversion using the EZ DNA Methylation kit (Zymo Research, Irvine, USA). Genome-wide DNA methylation was then performed using the Infinium HumanMethylationEPIC Bead Chip (Illumina) ...
-
bioRxiv - Microbiology 2023Quote: Viral RNA extraction from nasal washes of infected ferrets was done using the Quick-RNA Viral 96 Kit (Zymo, Cat. No. R1040) using 160 µL sample volume ...
-
bioRxiv - Microbiology 2023Quote: ... The MDA-amplified VLP DNA was then processed using the Zymo Genomic DNA Clean and Concentrator-10 kit (Zymo Research, CA, U.S.A) and eluted in Zymo DNA Elution Buffer ...
-
bioRxiv - Plant Biology 2024Quote: Total RNA from seedlings was isolated using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, Irvine, CA). cDNA was synthesized using Oligo(dT ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (500 x g for 5 minutes) and lysed for RNA and DNA extraction using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, D7003). RNA and DNA were eluted in water ...
-
bioRxiv - Molecular Biology 2024Quote: ... was used to purify genomic DNA from the cells followed by bisulfite conversion using 50 ng DNA along with the EZ DNA Methylation- Gold kit (Zymo Research D5005). 10 ng of bisulfite-converted DNA was used to generate the whole genome bisulfite sequencing (WGBS ...
-
bioRxiv - Microbiology 2024Quote: ... The amplified promoter and coding PIGJ DNA sequence were first purified from 1% agarose gels using the ZymocleanTM Gel DNA recovery kit (Zymo Research, #D4007) and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat. No. D4002, Zymo Research, Irvine, CA). A final pool was made after quantification of each sample using the Qubit high-sensitivity dsDNA kit (Cat ...
-
bioRxiv - Cancer Biology 2024Quote: RNA from K562 and Nalm6 cells was isolated using the NucleoSpin RNA Mini kit (Macherey Nagel) followed by DNase-digestion with the DNase I set (Zymo Research, #E1010) and clean-up using the NucleoSpin RNA Clean-up Mini Kit (Macherey-Nagel ...
-
bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
-
bioRxiv - Biochemistry 2023Quote: ... T7 transcribed SL5 RNA was first purified by polyacrylamide gel electrophoresis (PAGE) and recovered using small-RNA PAGE Recovery Kit (Zymo Research, R1070). RNase L (1.3 µg in 5 µL ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Samples were frozen in liquid nitrogen and mechanically homogenized before total RNA extraction was performed using the Quick-RNA™ Microprep Kit (Zymo Research). 200ng from each sample were transferred to GenTegra-RNA tubes (Syntezza Bioscience ...
-
bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
-
bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
-
bioRxiv - Genomics 2024Quote: ... RNA and DNA were simultaneously extracted from the cell samples using the Zymo Quick-DNA/RNA Microprep Plus Kit (Zymo Research, D7005) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research, Cat. No. D4033). The purified PCR amplicon products were sequenced by Eurofins Genomics ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
-
bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
-
bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were infected at 3 DIV with FL1 and at 10-12 DIV neurons were harvested and DNA extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
-
bioRxiv - Immunology 2023Quote: ... and total cellular RNA from cardiomyocytes isolated from vehicle-and Doxo-treated mice was prepared using Direct-zol RNA miniprep kit (Zymo Research, R2052). Next-generation RNA sequencing procedure for both sets of samples were performed at Texas A&M University Bioinformatics Core ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... and immediately lysed with TRIzol (Fischer Scientific, 15-596-018) for RNA isolation using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) per the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
-
bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets collected from 48-well plates were lysed and total RNAs were extracted using the Direct-zolTM RNA Miniprep kit (Zymo Research R2053) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... DNA extracts from each experimental replicate without standards or phage HM1 were cleaned up prior to library preparations with the Zymo Genomic DNA Clean and Concentrate-10 kit (Cat. No. D4011, Zymo Research Corporation). Long read libraries were prepared with the Ligation Sequencing Kit (Cat ...
-
bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from agar plugs (as described above for PFGE chromosome preparation) using the Zymoclean gel DNA recovery kit (Zymo Research, D4001T) and successively digested with Exonuclease V (RecBCD ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
-
bioRxiv - Evolutionary Biology 2023Quote: ... RNA was then extracted from each sample using the Quick-RNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, CA). The total amount of RNA in each sample was subsequently quantified with a Qubit 2.0 fluorometer (Life Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were then washed with PBS and lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA miniprep kit (Zymo Research, # R2050) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... 38% v/v saturated acidic phenol) and the Direct-zol RNA MiniPrep Kit (including on-column DNase I treatment, Zymo Research, R2052) or according to the protocol for TRIzol™ Reagent (Invitrogen) ...
-
bioRxiv - Biochemistry 2023Quote: ... equal amount of nuclease free water was added to each sample and purified using Oligo Clean & Concentrator kit (Zymo Research, CAT#: D4061). The samples were eluted in 6 μL of nuclease free water The samples were eluted in 6 μL of nuclease free water and an equal amount of 2x denaturing loading buffer was added for separation on a 20% denaturing polyacrylamide gel as described above ...
-
bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
-
bioRxiv - Immunology 2023Quote: ... we have isolated DNA for preparing Reduced Representation Bisulfite Sequencing (RRBS) libraries using Zymo-Seq RRBS Library Kit (Zymo Research, USA, #D5461). In brief ...
-
bioRxiv - Systems Biology 2023Quote: DNA was extracted from approximately 100 mg of each gut content sample using the ZymoBIOMICS™ 96 MagBead DNA Kit (D4308) (Zymo Research), following manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2023Quote: ... four independent differentiations from C71G+/- iMGs and three independent differentiations from M114T+/- and M114T+/+ iMGs were lysed with Trizol and total RNA was isolated with Direct-zol RNA Microprep Kit (Zymo Research, R2060) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: In vitro-infected keratinocytes and SCC cells were lysed in 6-wells with 1mL TRIzol Reagent and the RNA was purified by using the Direct-zol™ RNA Miniprep kit (Zymo Research). The procedure was performed according to the manufacturer protocol except for an additional 1 min centrifugation after the last washing step to completely remove eventual ethanol leftover ...
-
bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
-
bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
-
bioRxiv - Genetics 2023Quote: RNA was extracted from each of the 384 samples in batches of 30 samples using the Direct-zol RNA Miniprep kit (Zymo Research, R2050). Batches consisted of samples from the same tissue and were processed in the order individual samples were collected ...
-
bioRxiv - Neuroscience 2023Quote: ... We extracted DNA from the whole fly or from just the body (saving the heads for immunostaining from a subset of flies) using Quick-gDNA miniprep kit (Zymo Research R1050). We designed primers upstream and downstream of the Tra binding sites as follows:
-
bioRxiv - Molecular Biology 2023Quote: Genomic DNA from transduced primary cortical neurons or brain tissue from transduced animals was extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
-
bioRxiv - Genomics 2023Quote: ... bisulfite sequencing libraries were constructed according to the manufacturer’s instructions using the EZ DNA Methylation-GoldTM Kit (Zymo Research, Irvine, CA, USA). Bisulfite sequencing libraries were sequenced to generate 150 bp paired-end reads ...
-
bioRxiv - Genomics 2023Quote: ... 200 ng of sonicated DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold™ Kit (Zymo, Cat No. D5006). Libraries were then made using the Accel-NGS Methyl-Seq DNA Library Kit with unique dual indices following the size-selection guidance provided with the kit and 10 cycles of amplification to minimise clonality ...
-
bioRxiv - Bioengineering 2023Quote: ... and homogenized further using the mixer mill MM400 (Retsch) followed by RNA isolation with the Direct-zol™ DNA/RNA Miniprep Kit (Zymo Research). Total RNA concentrations were measured using the NanodropTM 2000c spectrophotometer (Thermo Fisher Scientific) ...