Labshake search
Citations for Zymo Research :
6051 - 6100 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 200-250µm-thick cryopreserved rat lung tissue using the Zymo Quick-DNA MicroPrep Kit (Zymo, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... All plasmids used for transient transfection experiments were purified with an endotoxin removal step (ZymoPURE Plasmid Miniprep Kit from Zymo Research). The PEmax** protein expression vector (pET-21a-PEmax**-6His ...
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bioRxiv - Plant Biology 2024Quote: Preparation of RNA samples from seedlings of the indicated genotypes and growth conditions was performed using a Quick-RNA MiniPrep Kit with on-column DNase I treatment (Zymo Research). cDNA synthesis was performed with 1.5 µg of total RNA using a Superscript IV First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from infected cells 72 h post infection using Quick-DNA Miniprep kit (Zymo Research, catalogue no. D3024).
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bioRxiv - Genomics 2024Quote: ... An aliquot of 250 ng DNA from each sample was bisulfite converted using the EZ DNA Methylation kit (Zymo Research, #D5001) according to manufacturer’s instructions for Illumina DNA methylation arrays ...
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bioRxiv - Microbiology 2024Quote: A whole-stranded transcriptome RNA library was generated using the Zymo-Seq RiboFree Total RNA kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... contains the Arabidopsis thaliana cytochrome P450 reductase I gene, ATR1) (Urban et al., 1997) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For plant transient expression ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µl of RNase free water was added to the mixture and transcribed RNA was eluted using the RNA Clean & Concentrator kit (Zymo Research). Samples were assessed for their length and concentration by Bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from freshly-grown colonies using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using the Nextera DNA Flex Library Prep as recommended by the manufacturer (Illumina ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and RNA was isolated using Direct-zol RNA miniprep Kit (Zymo research, cat. no. R2050). RNA was quantified and processed for cDNA synthesis using Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of every amplicon from the same RT product was pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products for both pairs of primers were pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from 1*106 WT or sorted SLAMF6-KO NK-92 cells using an RNA Micro Kit (Zymo Research). After PolyA selection libraries were prepared and lllumina sequencing of 20-30 million paired-end reads performed ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was collected over a time course of 24 hours and isolated using a Quick-DNA/RNA™ Miniprep kit (Zymo) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria expressing the correct plasmid were grown in bulk and high-purity plasmid was extracted using ZymoPURE Plasmid Midiprep kit (Zymo research) and verified by sequencing of the coding region of the influenza virus segment.
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using the manufacturer’s instructions using a YeaStar Genomic DNA kit (Catalog D2002, Zymo Research, Irvine, California, USA). Genomic DNA was subject to a PCR to check for the insertion of the corresponding tag or the CHC KO.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells expressing the iRS3 reporter 3h post-induction using the Direct-zol™ RNA MiniPrep kit (Zymo). After extraction ...
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bioRxiv - Zoology 2024Quote: ... and post-aestivating (55 days old) female beetles were extracted using the Quick-RNA Tissue/Insect Kit (Zymo Research, Freiburg, Germany). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA around binding sites was cleaved with pAG-MNase enzyme and the released DNA was reverse-crosslinked with proteinase K and 0.1% SDS overnight at 65°C before purification with a ChIP DNA Clean & Concentrator kit (Zymo Research D5205). DNA was converted into Illumina-compatible libraries with the NEBNext(R ...
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bioRxiv - Microbiology 2024Quote: ... 250 µl aqueous phase was mixed 1:1 with 75% ethanol and transferred into the Zymo-Spin IC Column from the RNA Clean & Concentrator Kit (Zymo Research). RNA clean-up and collection was done following the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... Two micrograms of DNA were subjected to sodium bisulfite conversion using an EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA). Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... The upper aqueous phase was transferred to an RNase-free tube and mixed in a 1:1 ratio with 100% EtOH and processed via the clean and concentrator kit (Zymo Research). RNA was eluted in 15 µL RNase-free ddH2O ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from 1-day old zebrafish embryos with TRI Reagent using Direct-zolTM RNA MicroPrep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Yeast transformations were performed either using a standard lithium acetate transformation or Zymo Frozen-EZ Yeast Transformation II Kit (Zymo #T2001) before plating on appropriate selection media82 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of ethanol were added to each of the samples before eluting the RNA using a Direct-zol RNA kit (Zymo Research) using manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Following MASC PCR validation using the MASC PCR primer set described in Supplementary Data 1 and BAC extraction using the ZR BAC DNA Miniprep Kit (Zymo Research) based on the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA from the heart and testis of the Madagascar ground gecko was purified using ISOGEN (#311-02501, Nippon Gene) and Direct-zol RNA Miniprep kit (#R2050, Zymo Research). The cDNA was synthesized from total RNA using Verso cDNA synthesis kit (#AB1453A ...
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted from callus and protoplast cultures using the ZR Plant RNA Miniprep™ kit (R2024, Zymo Research, CA, USA) and TRIzol™ Reagent (15596026 ...
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bioRxiv - Cell Biology 2024Quote: ... RNA from tdTomato positive SRSF2 knockout and P95H mutant cells and reporter positive control cells was extracted using the Direct-zol RNA microprep kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 1.5 mL cultures of Bacteroides strains using the Zymobionics DNA Miniprep kit (Zymo Research, Irvine, CA) and used as input for IS-Seq as described previously 19 ...
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bioRxiv - Systems Biology 2024Quote: ... We isolated the transposed and adaptor-ligated genomic DNA from the reaction with the Zymo DNA Clean and Concentrator--5 Kit (Zymo #D4014) and then amplified the libraries using the custom primers described in Supplemental Table 4 (originally designed by the William Greenleaf lab at Stanford University) ...
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bioRxiv - Synthetic Biology 2024Quote: Yeast genomic DNA was extracted from the potential aMF biosensor yeast strains and the unedited yWS677 strain (to be used as negative control in PCR analysis) using the YeaStar Genomic DNA kit and protocol (Zymo Research), with no known deviations.
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bioRxiv - Microbiology 2024Quote: Sterivex filters were extracted in-house at NOAA’s Atlantic Oceanographic and Meteorological Laboratory (AOML) using the ZymoBIOMICS 96 DNA/RNA MagBead kit (Zymo; CAT# D4308), with modifications for in-cartridge bead beating as described in (110) ...
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bioRxiv - Microbiology 2024Quote: ... they were subjected to rRNA depletion using Ribominus rRNA depletion kit and further purified using RNA Clean and Concentrator-5 columns (Zymo Research). RNA integrity was checked using Bioanalyzer before proceeding to library prep and sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was prepared from 1×106 cells of HAP1 and HELLS KO using Direct-zol RNA Microprep kit (Zymo Research). The RNA was checked for quality using the Agilent BioAnalzyer 2100 ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants of cells treated with compound 11 at 50 µM or mock-treated were collected three days after the infection and RNA was extracted using Quick RNA Viral kit (Zymo Research). The cDNA corresponding to the regions coding for E2 and E1 was obtained by RT-PCR using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... at which point cells were harvested and total DNA was extracted using a Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). qPCR was performed using a Quantstudio 3 (Thermo Fisher ...
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bioRxiv - Pathology 2024Quote: ... Adapter-ligated fragments of 150-250bp and 250-300bp were recovered from a 2/5% NuSieve 1:1 agarose gel (Zymoclean Gel DNA Recovery Kit, Zymo Research). The fragments were then bisulfite treated using EX DNA Methylation-Lightning Kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... The nested PCR products were then cleaned using a Zymo 96 well Clean and Concentrator Kit (Zymo Corp., Irvine, CA, USA).
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the fresh feces collected from UT1- or PBS-gavaged mice (female, n = 4/group) using a ZymoBIOMICS DNA Miniprep Kit (Zymo Research). Note ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from TRAP and input samples with TriZol and the Direct-Zol RNA MicroPrep Kit (Zymo Research, Orange, CA).
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bioRxiv - Plant Biology 2024Quote: ... Final GBKT7 plasmids was transformed into the yeast strain Y2HGold and transformants selected on SD medium lacking tryptophan (Trp) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Quantitative transactivation tests were performed as previously described (Ref) ...
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bioRxiv - Molecular Biology 2024Quote: ... A band from 250-400bp was cut and DNA was extracted in 150 μl of ddH2O using the Gel DNA Recovery Kit (Zymo D4007).
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bioRxiv - Genomics 2024Quote: ... Total RNA (∼700 ng) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA was mixed with ethanol absolute at a 1:1 v/v ratio and submitted to a spin column-based purification protocol (Direct-zol RNA Miniprep kit, Zymo Research), including a DNAse treatment step ...
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bioRxiv - Genetics 2024Quote: ... followed by resuspension of ∼200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Genomics 2024Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 × 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...
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bioRxiv - Plant Biology 2024Quote: ... Cleaned roots were frozen in liquid nitrogen and stored at −80 °C before DNA extraction with a ZymoBIOMICS 96 DNA Kit in plate (Zymo Research). Two grinding steps were performed ...
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bioRxiv - Plant Biology 2024Quote: ... DNA extraction was done using a Quick-DNATM fungal/bacterial miniprep kit following the manufacturer’s protocol (Zymo Research, Irvine, CA, USA). The extracted DNA quality was checked by loading 5 µl DNA + 2 µl 6× loading dye with gel red on a 1% agarose gel and separated at 100 V for 40 min ...
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bioRxiv - Physiology 2024Quote: ∼250 ng of total RNA from each sample was used as input to prepare libraries for total RNA-seq with Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research) following the manufacturer’s protocol ...