Labshake search
Citations for Zymo Research :
6201 - 6250 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Tissue RNA extraction was performed using the Direct-zol RNA miniprep plus kit with DNAase treatment per package insert (Zymo, Irvine, California). SIV and CD4 primers for RT PCR and amplification conditions were as previously published [28] ...
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bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: RNA was extracted from flash-frozen fetal liver (left lobe) samples using a Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA)) per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation of digested samples was performed using the Directzol RNA Miniprep kit with DNAse-I treatment to remove trace DNA before RNA elution (Zymo Research, R2050). RNA was quantified via Nanodrop spectrophotometry ...
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bioRxiv - Biochemistry 2022Quote: ... the LentiCRISPRv2 hygro plasmid was purified using a 0.7% agarose gel and a Zymoclean gel DNA recovery kit (Zymo Research cat # D4001). The prepared sgRNA oligo duplexes were then ligated into the purified LentiCRISPRv2 hygro plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... total cellular RNA using ∼1×106 cells from one well of a six-well plate was isolated using TRIzol reagent followed by Direct-zol RNA MiniPrep Plus kit purification (Zymo Research # R2070). RNA was quantified using Qubit RNA HS Assay kit and cDNA synthesised from ∼2µg of RNA using SuperScript IV VILO Master Mix kit following gDNA removal with ezDNase ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted and genomic DNA was removed using the Direct-zol 96-well MagBead RNA Kit (Zymo Research, Orange, CA). cDNA was synthesized from total RNA using the iScript™ Reverse Transcription System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... All samples were equilibrated to a total volume of 50 μL and then DNA was extracted using Zymo Quick-DNA Microprep Kit (Zymo Research, CA). Procedure was performed using modifications to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... were thoroughly mixed 1:1 with 2X DNA/RNA Shield™ and incubated at RT for 20 min for inactivation and vRNA was extracted using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Genomics 2020Quote: ... and 500ng of DNA from each sample was bisulfite converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol using the specified modifications for the Illumina Infinium Methylation Assay ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were verified by Sanger sequencing and purified with the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA). Two micrograms of plasmid DNAs were used to transfect small-scale HEKT293 cultures and optimize expression conditions both via mRuby2 visualization and western blot analysis ...
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bioRxiv - Microbiology 2021Quote: ... The fungal isolates were identified by extracting genomic DNA with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research; Irvine (Fig, CA), amplifying and sequencing the internal transcribed spacer (ITS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: DNA was extracted from 100 mg caecum content using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA, USA) with minor adaptations from manufacturer’s instructions (Ducarmon et al ...
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bioRxiv - Physiology 2021Quote: ... Total RNA was extracted from each supernatant with the Zymo Quick-DNA/RNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Plant Biology 2021Quote: ... The purified adapter-added genomic DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold kit (Zymo Research, Cat#: D5005). Then the treated genomic DNA was resolved in a 1.5% agarose gel ...
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bioRxiv - Bioengineering 2020Quote: ... Single-fly genomic DNA preps were prepared using the solid tissue protocol of the Quick-DNA™ Miniprep Plus Kit (Zymo Research). In total ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells were recovered by centrifugation and pGFP was purified using ZymoPURE™ Plasmid Maxiprep Kit (catalog #D4203, Zymo Research, Irvine, USA). The DNA concentration was measured in a NanoDrop™ 2000/2000c Spectrophotometer (ThermoScientific® ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
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bioRxiv - Bioengineering 2021Quote: ... control targeted cells (cells that underwent CRISPR/Cas9-mediated targeting and HDR of an expression cassette at a non-GSH locus) and two independent cultures of untargeted cells using Direct-zolTm RNA Miniprep kit (Zymo Research, ZYR.R2052). 1μg of RNA was converted into cDNA with Superscript IV Vilo MM (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: We extracted RNA and DNA from tissue biopsies using the ZR-Duet DNA/RNA MiniPrep Plus kit (Zymo Research, catalog number D7003). We performed the in-column DNase I treatment as specified in the kit to remove residual DNA from the RNA fraction ...
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bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted and genomic DNA was removed using the Direct-zol MagBead RNA Kit and following manufacturer’s protocol (Zymo Research, Orange, CA). RNA concentrations and contamination were determined spectrophotometrically using a Nanodrop (ND-1000 ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was purified and treated with DNase by using the Direct-zol RNA MiniPrep kit (Zymo Research Corporation, Orange, CA, USA), according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 20 mg of each tissue using the ZYMO Quick-DNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA). Resulting DNA was amplified neat and diluted 1:2 ...
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bioRxiv - Immunology 2021Quote: DNA for HLA typing was isolated from PBMCs using the Quick-DNA/RNA™ Microprep Plus Kit (Zymo Research, Irvine, CA, USA). High throughput-HLA typing was performed by the Institute of Transplantation Diagnostics and Cell Therapeutics (ITZ) ...
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bioRxiv - Immunology 2021Quote: A total of 200 ng genomic DNA was subjected to sodium bisulfite treatment and purified using the EZ DNA Methylation-Gold Kit (Zymo research, D5007) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2021Quote: Viral (v)RNA was extracted from the respiratory secretions of COVID-19 patients using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol and complementary (c)DNA synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™ ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from individual eGFP+ cell lines was extracted from 96 well-plates using Quick-DNA Universal 96 Kit (Zymo Research, D4071) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets collected from 48-well plates were lysed and total RNAs were extracted using the Direct-zolTM RNA Miniprep kit (Zymo Research R2053) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... DNA extracts from each experimental replicate without standards or phage HM1 were cleaned up prior to library preparations with the Zymo Genomic DNA Clean and Concentrate-10 kit (Cat. No. D4011, Zymo Research Corporation). Long read libraries were prepared with the Ligation Sequencing Kit (Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
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bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were infected at 3 DIV with FL1 and at 10-12 DIV neurons were harvested and DNA extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2023Quote: ... and total cellular RNA from cardiomyocytes isolated from vehicle-and Doxo-treated mice was prepared using Direct-zol RNA miniprep kit (Zymo Research, R2052). Next-generation RNA sequencing procedure for both sets of samples were performed at Texas A&M University Bioinformatics Core ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and immediately lysed with TRIzol (Fischer Scientific, 15-596-018) for RNA isolation using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Cell Biology 2023Quote: ... The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060). All RNA samples were initially amplified using the Ovation RNA-Seq System v2 kit (Tecan Genomics) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Bioengineering 2023Quote: Antibody genes were isolated from the terminal rounds of sorting against tau and α-synuclein fibrils using a yeast miniprep kit (Zymo, D2004). For tau ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Genomics 2023Quote: ... 200 ng of sonicated DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold™ Kit (Zymo, Cat No. D5006). Libraries were then made using the Accel-NGS Methyl-Seq DNA Library Kit with unique dual indices following the size-selection guidance provided with the kit and 10 cycles of amplification to minimise clonality ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from each of the 384 samples in batches of 30 samples using the Direct-zol RNA Miniprep kit (Zymo Research, R2050). Batches consisted of samples from the same tissue and were processed in the order individual samples were collected ...