Labshake search
Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2019Quote: ... 10 μm thickness muscle cryosections were incubated with 5 μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH; Molecular Probes, Eugene) and allowed to dry overnight at room temperature in dark ...
-
bioRxiv - Cancer Biology 2023Quote: ... in PBS/5% FBS at 37°C for 10 min or 2 μM H2-DCFDA (Thermo Fisher Scientific, C6827) in PBS/5% FBS at 37°C for 30 min ...
-
bioRxiv - Bioengineering 2023Quote: ... cerevisiae cells (OD 5-10) were pelleted and lysed in 200 µL 0.9% physiologic salt supplied with 2 µL RNAse cocktail (Invitrogen) and 5mg/mL Zymolyase 100T (MP Biomedicals) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and one wash with 50 μl of 5× Maxima H RT buffer (Thermo Fisher). Finally ...
-
bioRxiv - Neuroscience 2020Quote: In vivo mitochondrial ROS production in immunostained microglia was measured by injecting dihydro-ethidium (DHE; Invitrogen by Thermo Fisher Scientific, D11347), as it is specifically oxidized by superoxide to red fluorescent ethidium ...
-
bioRxiv - Neuroscience 2020Quote: In vivo mitochondrial ROS production in immunostained microglia was measured by injecting dihydro-ethidium (DHE; Invitrogen by Thermo Fisher Scientific, D11347), as it is specifically oxidized by superoxide to red fluorescent ethidium ...
-
bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU; 5 mg per kg body weight, Invitrogen) dissolved in sterile phosphate buffer solution (PBS ...
-
bioRxiv - Microbiology 2023Quote: ... 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated into a PCR by the use of a DreamTaqTM DNA Polymerase (Thermo Scientific™ ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then treated with hexamethyldisilazane-trimethylchlorosilane-pyridine solution (3:1:9; ThermoFisher) for 20 min at 110°C ...
-
bioRxiv - Biophysics 2020Quote: ... Then 1 mL dye solution was mixed with 9 grams glycerol (15514011, Invitrogen) and added 400 μl solution to a home-made sample holder for 3D-SMART experiments (Supplementary Fig ...
-
bioRxiv - Cell Biology 2022Quote: ... were transfected with 1-9 µg of bacmid DNA using ExpiFectamine (Life Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... mounted with fluorescence mounting medium (9 ml of glycerol [BP229-1; Fisher Scientific] ...
-
bioRxiv - Cancer Biology 2023Quote: ... mounted with fluorescence mounting medium (9 ml of glycerol [BP229-1; Fisher Scientific] ...
-
bioRxiv - Cancer Biology 2023Quote: ... mounted with fluorescence mounting medium (9 ml of glycerol [BP229-1; Fisher Scientific] ...
-
bioRxiv - Neuroscience 2021Quote: ... fluorescently conjugated Cholera toxin subunit B (CTB-555, 2 μg/μl, 300 nl, Invitrogen) was injected unilaterally into VLS ...
-
bioRxiv - Neuroscience 2021Quote: ... in Neurobasal medium enhanced with 2% B-27 and 0.5% L-Glutamine (Life technologies). Primary hippocampal neurons growing on either 12mm or 18mm coverslips ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with B-27 and 2 mM Glutamax (Gibco; Thermo Fisher, Waltham, MA, USA). Primary neuronal cultures were infected with AAV-ESARE-mScarlet-Flag particles at a titer of 4.35×106 genome copies/mL and AAV-CaMK0.4-FHS-paCaMKII-WPRE3 at a titer of 4.72×106 genome copies/ml at DIV 9 ...
-
bioRxiv - Neuroscience 2020Quote: Fluorescently conjugated Cholera toxin subunit B (CTB-555, 2 μg/μl, 300 nl, Invitrogen) was injected unilaterally into V1 (AP ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with B-27 and 2 mM Glutamax (Gibco; Thermo Fisher, Waltham, MA, USA). Primary neuronal cultures were infected with AAV-ESARE-mScarlet-Flag particles at a titer of 4.35×106 genome copies/mL and AAV-CaMK0.4-FHS-paCaMKII-WPRE3 at a titer of 4.72×106 genome copies/ml at DIV 9 ...
-
bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Recovered nuclei were stained with FITC-conujugated α-5-bromo-2’-deoxyurine (Invitrogen, MoBu-1) in staining buffer (2 mM HEPES pH 7.4 ...
-
bioRxiv - Developmental Biology 2023Quote: Tadpoles were immersed in a solution containing 1 mM EdU (5-ethynyl-2’-deoxyuridine; Invitrogen) before fixation ...
-
bioRxiv - Genetics 2021Quote: ... at 85 V for 2 hours 15 minutes at an ambient temperature of 4 °C in NuPAGE™ Transfer Buffer (Invitrogen, UK) containing 10% 2-propanol (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies or reagents were used ...
-
bioRxiv - Neuroscience 2021Quote: ... 20 µl of either type of samples were run on BOLT 4-12% Bis-Tris mini protein gels for SDS-PAGE and electroblotted using the iBlot 2 dry blotting system (Thermo Fisher, USA). Polyvinylidene fluoride membranes were probed with anti-PrP monoclonal antibody ICSM-18 (1:4,000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2019Quote: ... staining was performed in three-dimensional cysts with 2 mM calcein AM and 4 mM ethidium homodimer I (Live/Dead® Viability/Cytotoxicity kit; Life Technologies) for 40 min at 37°C in the dark ...
-
bioRxiv - Cancer Biology 2019Quote: Cells were air-dried and fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 20 minutes on glass slides using Shandon cytospin 2 (THERMO FISHER SCIENTIFIC). Fixed cells were permeabilized ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples were heat-denatured at 98°C for 8 minutes in denaturing Laemmli buffer (containing 2-mercaptoethanol) and resolved on 4–12% gradient Invitrogen Bolt Bis-Tris Plus gels (Thermo Fisher Scientific). The resolved proteins were transferred via wet transfer in buffer (1X Tris-glycine ...
-
bioRxiv - Cancer Biology 2019Quote: ... The molecular sizes of peak 1 (300 kDa) and peak 2 (150 kDa) were determined by SDS gel electrophoresis (NuPAGE 4-12% Bis-Tris Gel; Life Technologies, CA) under non-reducing conditions along with antibody standards.
-
bioRxiv - Neuroscience 2019Quote: ... All the fluorescent indicators employed in the study (fura-2 AM, fluo-4 AM, hydroethidine, TMRE, and MitotrackerGreen FM) were obtained from Molecular Probes (ThermoFisher). Unless otherwise specified ...
-
bioRxiv - Neuroscience 2020Quote: ... Embryo hippocampi were dissected in 4 °C Hibernate E supplemented with 2% B27 supplements and 100 U/mL Penicillin/Strep (Thermo Fisher Scientific). Hippocampal tissues were digested in Hibernate E containing 20 U/mL papain ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
-
bioRxiv - Cancer Biology 2019Quote: ... washed extensively with phenol red-free RPMI containing 2% FBS and stored overnight at 4°C prior to staining with CellEvent Senescence Green (Thermo Fisher Scientific). Flow cytometric analysis and sorting was performed at the Flow Cytometry shared Resource Facility at the Icahn School of Medicine at Mount Sinai ...
-
bioRxiv - Biochemistry 2021Quote: ... Grids were then blotted with filter paper for 2 s at 100 % humidity at 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with PBS and mounted on the slide with ProLong Diamond antifade with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies, #P36966). Confocal images were acquired on a Nikon Ti-Eclipse A1M microscope fitted with a 60× oil immersion objective using 488 nm ...
-
bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... formic acid and the peptides equivalent to 2-4 µg of proteins were loaded for mass spectrometry analysis on a Orbitrap Elite mass spectrometer (ThermoFisher Scientific Inc.). The separation of peptides was performed on an analytical column (75 µm × 15 cm ...
-
bioRxiv - Molecular Biology 2020Quote: ... Ten-microliter sample were loaded at a 0.45 mL/minute flowrate onto an anion separator (2 × 250 mm, 4 μm, AS19, Thermo Scientific, Bremen, Germany) with a 50 mm guard with the same separator material ...
-
bioRxiv - Cancer Biology 2020Quote: 10 ul whole cell extracts of 2 × 105 cells in 40 μl 6X SDS loading buffer were run on 4-14% bis-tris gel (Invitrogen cat # NP0335). Membranes were transferred by semi-dry apparatus (Bio-Rad Transblot cat # 170-3940 ...
-
bioRxiv - Biophysics 2020Quote: ... The grids were blotted for 4 s or 2 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 4°C and 100% humidity ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were loaded with the Ca2+ indicator Fluo-4/AM (for intracellular Ca2+) or Rhod-2/AM (for mitochondrial Ca2+) (Thermo Fisher Scientific) for 15 min at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: HEK293T cells plated at a density of 80,000-90,000 cells.cm-2 on a pre-coated PDL 4-well Nunc™ Lab-Tek™ II chamber (Thermo Fisher Scientific, #155382PK) were co-transfected with PylRS/4xtRNAPyl (500 ng ...
-
bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cell Biology 2022Quote: ... Slides were washed three times and ProLong® Gold Antifade mounting reagent containing DAPI (4’,6-diamidino-2-phenylindole) (Thermo Scientific, #P10144) was applied to mount the coverslips.
-
bioRxiv - Bioengineering 2022Quote: ... 15-day and 30-day 3T3-L1s adipocytes were stained for neutral lipids using DMEM 10% FBS 1% Anti/Anti culture media supplemented with 2 μM BODIPY 493/503 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, D3922; Invitrogen, Waltham, MA, USA). Cells were first rinsed with DPBS ...
-
bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
-
bioRxiv - Microbiology 2021Quote: ... then 50 μl of the EV preparations were mixed with 250 μl PBS staining buffer containing 2.5 µg/ml DAPI (4’, 6-diamidino-2-phenylindole; Thermo Fisher Scientific, USA)) and kept at 4 °C for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 μl of BalCP20-P3 sample prepared as described above was mixed with an equal volume of gradient DTT (4∼200 mM) and 20 μl Tricine SDS Sample Buffer (2×) (Thermo SCIENTIFIC, USA) and placed in a water bath at 70°C for 10 min ...
-
bioRxiv - Neuroscience 2022Quote: ... P-1149)-coated 96-well and 24-well plates were seeded with 4 × 104 and 2 × 105 cells accordingly in neurobasal medium (Invitrogen, 21103-049) supplemented 5 ng/ml HBEGF (Sigma-Aldrich ...