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Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Samples (input (eluted in 2× SDS buffer and thawed 15 minutes at 75°C) and IP proteins) were run in NuPAGE 4%–12% Bis-Tris Mini Protein Gel (10 wells) (ThermoFisher Scientific, NP0321BOX) at the voltage of 100V for 2.5 hours in a NuPAGE MOPS SDS Running buffer (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Four milliliters of each bacterial resuspension in ice-cold PBS were pelleted by centrifugation (14000 g, 10 min, 4 °C) (Heraeus Fresco 21, Thermo Fisher Scientific). The cells were then resuspended in 4 mL of PBS containing 2.5% sodium dodecyl sulfate (SDS) ...
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bioRxiv - Genomics 2023Quote: Equal amounts of lysate were denatured for 10 minutes at 80°C and loaded on a 4 to 12% polyacrylamide Bis-Tris gel (Thermo Scientific #NW04120BOX). The gel was run at 120V for approximately 80 minutes in MOPS buffer ...
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bioRxiv - Biochemistry 2023Quote: Coverslips plated with NRVM (-/+10 and 50 μM H2O2 for 48 hr) were fixed with 4% paraformaldehyde (PFA, Fisher Scientific, Cat#15710) in PBS for 20 min at RT and permeabilized with 0.1% Triton X-100 (Acros ...
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bioRxiv - Microbiology 2023Quote: ... The TEV cleavage reaction was incubated at 4°C for 16 hours in a 30 mL 10 kDa molecular weight cutoff dialysis cassette (ThermoFisher Scientific, 66830) dialyzing against 2 L TEV reaction buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 14000 g for 10 min and loaded onto a NuPAGE Novex 4-12% Bis-Tris gel (Novex Life Technologies, WC1020) and run at 120 V for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... at 70°C for 10 min and then electrophoretically separated by SDS-PAGE in 4-12% Bis-Tris precast gels in MOPS buffer (Invitrogen/Thermo-Fisher). Gels were transferred to PVDF membranes using the iBlot2 semi-dry transfer device (Invitrogen/Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were obtained via centrifugation at 20,000 ×g for 10 min at 4°C and were incubated with Purified GST-RhoA (G17A)-glutathione magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein lysates (10 μg/lane) were fractionated on a NuPAGE Novex 4%–12% Bis-Tris Protein Gel (Thermo Fisher Scientific) and transferred onto a PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... 58 were spiked into each sample prior to loading onto a Fe3+-IMAC column (Propac IMAC-10 4×50 mm, Thermo Fisher Scientific). The enrichment was performed with Buffer A (0.07% TFA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were boiled at 70°C for 10 minutes prior to loading on a 4%-12% NuPAGE Bis-Tris gel (Thermo Scientific, #NP0321). The gel was run in 1x MOPS buffer at 180V for 10 minutes and subsequently fixed and stained with Coomassie G250 (Carl Roth) ...
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bioRxiv - Bioengineering 2023Quote: ... LNPs were dialyzed against 1x PBS for 4 hours using Slide-A-Lyzer™ G2 Dialysis Cassettes with a 10 kDa molecular weight cut-off (87730, Thermo Fisher). Formulations were stored at 4°C until use.
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bioRxiv - Immunology 2024Quote: ... were coated overnight at 4 C with 10 ug/mL of antigen (INS, IA2, GAD, TET, DNA, or PE (Invitrogen, Cat#P801)) in 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10-30 μg of proteins were loaded and resolved on either a 10% or 4-12% Bolt Bis-Tris gel (Thermo Fisher Scientific), transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were heated at 100°C for 10 minutes and loaded on a NuPAGE 4%–12% Bis-Tris gel (Thermo Fisher Scientific). Afterward ...
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bioRxiv - Molecular Biology 2024Quote: ... at 300 × g for 10 min at 4 °C and washed twice by centrifugation with cold Dulbecco’s Phosphate Buffered Saline (D-PBS; Thermo Fisher 14190-144). Cell pellets were then resuspended in 600 µL of mirVana Lysis Buffer and RNA was isolated according to the kit manufacturer’s protocol with elution in a final volume of 100 µL.
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated for 10 min at 95 °C and were immediately loaded in 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and ran at constant 120 V (see below).
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bioRxiv - Neuroscience 2024Quote: ... then maintained in culture for 4 days in growth medium (DMEM [Thermo Fisher Scientific 11960044] supplemented with 10% heat inactivated FBS [Thermo Fisher Scientific 10437028] ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 10 ^5 TC32 or A673 cells (mixed in a 1:1 solution of growth factor reduced Geltrex [Life Technologies, catalog number A1413202] with PBS) were injected into the mouse in a peritibial location.
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bioRxiv - Biochemistry 2021Quote: ... at a concentration of 2 × 105 cells ml−1 (2 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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bioRxiv - Cell Biology 2023Quote: Plasma from LPS-treated mice were diluted 1:10 with Hanks’ Balanced Salt Solution (no Ca+2/Mg+2; Gibco) in 96-well black MaxiSorp™ plate ...
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bioRxiv - Bioengineering 2020Quote: ... ITS+1 (10 µg/mL insulin, 5.5 µg/mL transferrin, 5 ng/mL selenium; Gibco), 100 µM ascorbic acid 2-phosphate (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... After this 1⨯10 5 BMDC per condition were cultured on a collagen (Fisher Scientific) coated6-well plate (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... with a feeding of 10% (v/v) CHO CD EfficientFeed B (Thermo Fisher Scientific) on days 3 ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml of recombinant trimeric S protein (50ul per well ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 µg/ml of recombinant trimeric S protein (50 µL per well ...
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bioRxiv - Biophysics 2021Quote: ... The AutoScript 4 Software is commercially available from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2020Quote: SDS-PAGE electrophoresis (4-12% Bis-Tris gel; Life Technologies) demonstrating Ni-NTA purification of nsp10 and nsp16 ...
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bioRxiv - Cell Biology 2020Quote: ... target cells were selected using 4 μg/ml puromycin (ThermoFisher) or 150 μg/ml zeocin (Invivogen ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by NuPAGE 4%–12%gradient gels (Invitrogen), transferred onto PVDF membranes (Immobilon®-P ...
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bioRxiv - Immunology 2021Quote: ... and were loaded on a 4-12% NuPAGE gel (Invitrogen). Electrophoresis was performed in an MES buffer at 200V for 35 min ...
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bioRxiv - Immunology 2021Quote: ... NK-92 cells were loaded with Fluo-4 NW (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... Right after centrifuging from the cytocentrifuge (Cytospin 4, Thermo Scientific), fresh BAL cell cytospin slides were stained using a Kwik-Dif Stains kit (Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... and run on 4-12% gradient Tris-glycine gels (Invitrogen). The proteins were transferred onto a nitrocellulose membrane (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 96-well flat bottom Immulon 4 HBX plates (ThermoFisher Scientific) were coated with 150 μl/well of fetuin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were collected and fixed in 4% paraformaldehyde (Fisher Scientific) overnight before being processed through graded ethanols and xylene and then embedded in paraffin as previously described (Tavares et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and resolved using NuPAGE 4-12% Bis-Tris gels (Invitrogen). Proteins were transferred to PVDF membranes and immunoblotted ...
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bioRxiv - Neuroscience 2021Quote: ... cryoprotected at 4°C in 30% sucrose (Thermo Fisher Scientific) for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... BMEs were labeled with FM 4-64 (Molecular Probes, Inc.) or by labeling RNA cargos by using the ExoGlow-RNATM EV Labeling Kit (System Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Hive Five cells (BTI-TN-5B1-4) (Thermo Fisher Scientific) were cultured in ESF921 medium (Expression Systems ...
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bioRxiv - Neuroscience 2021Quote: ... 4 ng/mL Alexa Fluor-647-conjugated azide (ThermoFisher, A10277), and 100 mM sodium ascorbate (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and eluted by 4× lituium dodecyl sulfate (LDS; Invitrogen, NP0008). Membranes were immunoblotted with anti-V5 (ab15528) ...
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bioRxiv - Molecular Biology 2020Quote: ... quality and quantity were measured by Qubit 4 fluorometer (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... run on 4-12% NuPAGE™ Bis-Tris gel (Invitrogen) and transferred on to Nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... The concentration is determined using a Qubit 4 Fluorometer (ThermoFisher). The UMI labeling step is done by one round of primer extension with a high-fidelity DNA polymerase ...
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bioRxiv - Bioengineering 2022Quote: ... The brain slices were fixed by 4% formaldehyde (Thermo fisher) for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) and stained with SYPRO-Red Protein Gel Stain (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Lysates were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) for 50 minutes at 180V and transferred to polyvinylidene difluoride (Immobilon-FL ...
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bioRxiv - Immunology 2021Quote: ... Clear flat-bottom 96-well Immulon 4 HBX plates (ThermoFisher) were coated with 50 μl of 2 μg/ml of cH6/1 diluted in ELISA coating buffer (50 mM Na2CO3 ...