Labshake search
Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... The coupon was incubated in a 300 nM (100 ng/mL) solution of DAPI (4’,6-diamidino-2-phenylindole, ThermoFisher, Waltham, MA) for 3 minutes as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were plated on laminin-coated 18 mm coverslips and treated with 2 μm Rhod2-AM and 4 μm Fluo4-AM (both from Thermo Fisher Scientific) for 15 min at 37°C in external solution (145 mM NaCl ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were fixed for 1 h in PBS with 4% PFA and Streptavidin conjugated to Alexa 647 (1:500, ThermoFisher Scientific) was applied during secondary incubation following blocking and permeabilization.
-
bioRxiv - Bioengineering 2019Quote: ... at 4°C followed by AlexaFluor-568 antibody (1:500) and concurrently with AlexaFluor-647-conjugated bungarotoxin (1:1000, Invitrogen, B35450) for two hours at room temperature ...
-
bioRxiv - Bioengineering 2019Quote: ... NB100-2284) overnight at 4°C followed by AlexaFluor-568 antibody (1:500) or AlexaFluor-568 antibody (1:500, Invitrogen, A10087), respectively ...
-
bioRxiv - Immunology 2021Quote: 293T cells were co-transfected with pHDM-Wuhan-Hu-1 and pMAX-GFP plasmids at a 4:1 ratio by lipofectamine 3000 (Thermo fisher). 293T cell pool cotransfected with pHEF-VsVg and pMAX-GFP plasmids at a 4:1 ratio was used as a negative control ...
-
bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
-
bioRxiv - Immunology 2021Quote: ... 5×105 murine or human T cells have been stimulated at 1:1 ratio in 48well plate in presence of Sulfate Latex 4% w/v 5µm Beads (Molecular Probes S37227). For each experiment ...
-
bioRxiv - Microbiology 2021Quote: ... antibody 1:3000 in 1% PBS/BSA at 4°C followed by secondary antibody conjugated to Alexa Fluor 555 (Thermo Fisher) at 1:500 in PBS/BSA 1% was performed ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
-
bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Larvae were rinsed in PBST three times for 20 min and incubated overnight at 4°C with secondary antibodies (anti-mouse 1:500 IgG-Alexa Fluor 488, 1:500 IgG Alexa Fluor 594, Molecular Probes; anti-rabbit IgG-Alexa Fluor 488 ...
-
bioRxiv - Bioengineering 2019Quote: ... The respective spectra (average from 200 scans at 4 cm−1 resolution) were recorded between 4000-650 cm−1 using an FTIR Nicolet Nexus 470 instrument (Thermo Scientific), purged with dry ...
-
bioRxiv - Physiology 2020Quote: ... primary adipocytes were attached to poly-L-lysine (25-100 μg ml-1) coated coverslips and incubated with the Ca2+ fluorophore Fluo-4 AM (1 μM; Molecular Probes) in modified Hank’s solution for 1 hour at 21-23°C in the dark ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the RPE sheets/fragments were resuspended in 4 ml RPE media (1:1, DMEM3: Ham’s F12 medium (GIBCO 31330-038), 10% (v/v ...
-
bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041; AlexaFluor 647, 1:200, Invitrogen, A21236). Whole muscles were dehydrated in a series of 25% ...
-
bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C for 18 hours with primary antibodies: E-Cad (13-1700, Thermo Fisher, HECD-1, 1:100) and CK-14 (RB-9020 ...
-
bioRxiv - Biophysics 2023Quote: ... blocked and imaged as described under section triple-color immunostainings but were incubated with mouse anti-AnkG (#AB_10675130, Neuromab, 1:200) overnight at 4°C and subsequently with donkey anti-mouse AF647 (#A31571, Invitrogen, 1:800) for 1h.
-
bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were washed and suspended with 1 mL of 4% FBS/PBS containing DAPI (1:2000; Thermo Fisher Scientific, 62248) and DRAQ5 (1:5000 ...
-
bioRxiv - Biochemistry 2023Quote: ... containing 1 mM EDTA and 1 mM dithiothreitol were reacted with a 4-fold concentration of Protein Thermal Shift Dye (Life Technologies) in a 20 μl reaction volume ...
-
bioRxiv - Neuroscience 2023Quote: ... 4) with secondary Abs anti-mouse or rabbit IgG conjugated Alexa 488 (1:400) or Alexa 568 (1:200) (Life Technologies).
-
bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
-
bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
-
bioRxiv - Cancer Biology 2024Quote: ... these plasmids were transfected into HEK293T cells alongside psPAX2 and pMD2.G at a mass ratio of 1 pMD2.G : 3 psPAX2 : 4 pLKO.1 using Lipofectamine 2000 (Invitrogen 11668019), and media containing the lentivirus was concentrated with Lenti-X Concentrator (Takara Bio 631232 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged 1:10 every 2–3 days with 1× trypsin-EDTA 0.25% (Gibco 25200-056).
-
bioRxiv - Pathology 2021Quote: ... Larvae were injected in the common cardinal vein with 1 nl of Cy3-β-Lactoglobulin (5 mg/ml) and/or Alexa fluor™ 488 10 kDa dextran (D22910, Thermo Fisher, at 2 mg/ml) diluted in sterile H2O and successful injections were screened for on a Leica Mz10F fluorescent stereomicroscope ...
-
bioRxiv - Neuroscience 2020Quote: ... HA-AP2M1 and eGFP were co-transfected at a plasmid ratio of 10:10:1 into neurons at DIV 5 using Lipofectamine 2000 reagent (Invitrogen). At 48 hours post-transfection ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... using standards covering a range from 10-1 to 10-5 ng/µl fungal DNA (measured using a NanoDrop spectrophotometer, Thermofisher). Each run included the standards and a negative control ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’) and 1 μl of 10 mM dNTP mix (Thermo Fisher). Illumina libraries were prepared by using a modified smart-seq2 protocol [Picelli 2014] using SuperScript IV RT and tagmentation procedure as previously described [Henning 2018] ...
-
bioRxiv - Cell Biology 2023Quote: ... 9 µL of the reaction was stopped with 9 µL NuPAGE LDS Sample Buffer (Invitrogen) at 0 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
-
bioRxiv - Physiology 2023Quote: ... with a 1:5 – 1:10 passaging rate onto cultureware coated with Geltrex (LDEV-Free, hESC-Qualified; Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... IALVs were then washed with PBS containing 0.1% Triton X-100 (PBST) 3 times and blocked for a minimum of 2 hr with Blockaid® (B-10710, ThermoFisher Scientific). IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution ...
-
bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 500 g for 5 min at 4°C and the cell pellet was incubated with ACK lysing buffer (Thermo Fisher Scientific, A1049201) for 5 minutes to lyse the red cells ...
-
bioRxiv - Molecular Biology 2022Quote: To control efficiency of the enzymatic treatment 5 μg of the enzyme-treated products or controls were run on a NuPage™ 4-12 % Bis-Tris gel (Invitrogen, Carlsbad, USA) at 100 V for 60 minutes using the EI9001-XCELL II Mini Cell (Novex ...
-
bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...
-
bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
-
bioRxiv - Cell Biology 2021Quote: ... cells were seeded 4–5 × 104 cells per well in 8-well Lab-Tek II chambered cover glass slides (Nunc, Rochester, NY, USA) and incubated overnight ...
-
bioRxiv - Systems Biology 2022Quote: ... and the resulting supernatant (100 ng/ml) was incubated for 4 h at 37°C together with 5 mM glutamine (Gibco, Cat# 25030-081), 1 mM phosphoribosyl pyrophosphate (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cell suspensions were placed in clean cytospin cones and spun at 600g for 5 min on an Epredia Cytospin 4 (Fisher Scientific Cat#A78300003) to charged glass slides ...
-
bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with SpK (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were sonicated and heated for 5 min at 95°C before loading onto a Novex™ WedgeWell™ 4-20% Tris-Glycine Gel (ThermoFisher #XP04200BOX). Proteins were transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Molecular Biology 2020Quote: ... To clear the homogenate from debris and larger worm fragments it was centrifuged at 800 g for 5 minutes at 4°C (Heraeus Fresco 21, Thermo Scientific, Langenselbold, Germany) and the supernatant collected ...