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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... containing 5 - 10% FBS (Thermo Fisher Scientific). All cells were maintained at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... Samples were heated at 100 °C for 10 min prior to electrophoresis through a Novex NuPAGE 4–12 % Bis-Tris gel (Life Technologies, # NP0335BOX) in MES SDS running buffer (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled in 95°C for 10 minutes and loaded into NuPage Novex 4-12% Bis-Tris SDS-PAGE Gels (Thermo Fisher Scientific), electroblotting was performed using the Biorad Trans-Blot Turbo Transfer System ...
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bioRxiv - Molecular Biology 2020Quote: Mature segmented schizonts from RAP- and DMSO-treated cultures were Percoll enriched and ∼1.25×108 cells from each condition incubated in phenol red free RPMI containing 10 μM Fluo-4-AM (Invitrogen, California, United States) in the dark at 37°C for 45 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then cleared by centrifugation for 10 minutes at 12,000 x g at 4°C in a Sorval SS-34 fixed-angle rotor (Thermo Scientific, Waltham, MA). The supernatant was then incubated with glutathione-agarose (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... Therefore the cells were fixed with 4% PFA in the HydroDish® and nuclei stained with 10 μM Hoechst 33342 (H1399; Thermo Fisher) in PBS ...
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bioRxiv - Genetics 2020Quote: ... were added to the samples These were heated at 70°C for 10 minutes and loaded onto 4-12% Bis-Tris gel (Thermo Fisher, NW04125BOX). The proteins were separated with 150V electrophoresis for 30 minutes and blotted onto a PVDF membrane using the iBlot Dry 2 blotting system ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated at 70°C for 10 minutes and resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific) followed by electroblotting onto Immun-Blot PVDF Membrane (Biorad ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 to 30 μg of total protein was loaded onto NuPAGE Bis-Tris 4-12% acrylamide gels (Thermo Fischer Scientific) along with prestained protein ladder PageRuler (10-180 kDa) (Thermo Fisher Scientific) and run at 100 V ...
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bioRxiv - Cell Biology 2021Quote: ... and Reducing Agent (NuPAGE™ Sample Reducing Agent (10X) Invitrogen NP0004) at 70°C for 10 minutes before loading in NuPAGETM 4-12% Bis-Tris Gels (Invitrogen NuPAGE NP0336). 1x MOPS (BOLT Invitrogen B000102 ...
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bioRxiv - Microbiology 2020Quote: ... lysates from 106 cells were immunoprecipitated using 4 µg anti-SLC35A1 antibody and Protein G magnetic Dynabeads (Thermo Scientific, 10-003-D) overnight at 4C prior to immunoblotting to enhance signal and specificity of signal.
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bioRxiv - Microbiology 2021Quote: ... 10 µg of the whole cell lysate was separated using NuPAGE 4−12% Bis-Tris gradient gels (Invitrogen Life Sciences, Carlsbad, CA) and transferred to a PVDF membrane (Millipore ...
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bioRxiv - Systems Biology 2020Quote: ... the cells were washed with PBS and fixed for 10 minutes with 4% formaldehyde (Image-iT fixative solution, Thermo Fisher Scientific, FB002) at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were loaded onto a gel and separated by SDS-PAGE using NuPAGE® pre-cast gels (10 % or 4-12 %) (Thermo Scientific). Protein was transferred onto PVDF membrane and blocked and incubated with primary antibody in 5 % non-fat milk or BSA diluted in PBS-Tween 20 ...
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bioRxiv - Immunology 2022Quote: ... Grids were blotted from the backside for 10 seconds at 4℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). We removed one of the blotting arms for one-side blotting because Birbeck granules would be absorbed onto the filter paper ...
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bioRxiv - Molecular Biology 2020Quote: ... unit of cells in a 1X Laemmli/8 M Urea/ 2.5 % β-mercaptoethanol sample buffer and incubated for 10 minutes at 70 °C prior to SDS-PAGE using 4-12 % Bis-Tris gels (Invitrogen NuPAGE®, Thermo Scientific) in a MOPS buffer (Invitrogen NuPAGE® ...
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bioRxiv - Microbiology 2021Quote: ... Sorted cells were centrifuged for 10 minutes at 1,000×g and pelleted on a glass slide using a cytospin 4 cytocentrifuge (Thermo Fisher scientific, USA). Glass slides were air dried ...
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bioRxiv - Developmental Biology 2022Quote: ... but the blastocysts were incubated in 10 μM 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC; Cell Tracker Blue, Life Technologies, Carlsbad, USA). Fluorescence intensity for GSH was measured under a Nikon scanning confocal microscope with a filter at 371 nm excitation and 464 nm emission ...
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bioRxiv - Genetics 2022Quote: ... #NP0007)—and boiled at 95°C for 10 minutes before the samples were loaded in the NuPAGE 4%–12% Bis-Tris Gels (Invitrogen, #NP0335BOX & #NP0336BOX) for further resolution and western blot analysis ...
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bioRxiv - Neuroscience 2019Quote: ... the pH was adjusted to 7.4 using 1 M Tris pH 8 and then dialyzed overnight at 4°C in Hartmann’s using a 10 kDa dialysis membrane (Fisher Scientific, Loughborough, UK). The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay ...
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Neuroscience 2020Quote: ... Extracts were centrifuged at 15.000g for 10 min at 4°C and supernatants were collected and quantified with Pierce™ BCA Protein Assay Kit (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... containing 4.5 g/L glucose, 4 mM stable glutamine, and sodium pyruvate (Biochrom, Berlin, Germany) supplemented with 10% inactivated fetal calf serum (iFCS; Gibco, Darmstadt, Germany) in an atmosphere of 5% CO2 and 90% humidity at 37 °C.
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bioRxiv - Neuroscience 2020Quote: Samples (20 µL containing 10 µg protein) were loaded per well of a NuPage 4-12 % Bis-Tris gradient gels (#NP0336BOX, Thermo Fisher Scientific), and the SDS-Page gel was subsequently transferred to nitrocellulose membranes (#1620115 ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were post-fixed with 4% PFA in 1x PBS for 15 minutes followed by three 10-minute washes in 2x SSC (Thermo Fisher, 15557036). The samples were dried and hybridized for 24-36 hours with the probe library (~2.5 nM per probe) ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... cells were fixed with 4% paraformaldehyde (PFA) pH 7.4 at room temperature for 10 min and washed 3 times in PBS (Gibco Life Technologies,10010) for 5 min each ...
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bioRxiv - Neuroscience 2019Quote: ... mice were anesthetized with Euthasol euthanasia solution and transcardially perfused with ice cold heparinized physiological saline (10 units heparin/mL physiological saline) followed by 4% paraformaldehyde (PFA, Fisher Scientific #AC416785000) in 0.1 M phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... Solubilized material was centrifuged at 20,000 g for 10 minutes at 4°C and the supernatant was precleared with protein A/G sepharose beads (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were subjected to HPLC (LC Packing Famos) on a Fe-IMAC column (4 × 50 mm ProPac IMAC-10, Thermo Fisher Scientific) with peptide eluted in Buffer A (30% MeCN ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were boiled at 70°C for 10 min and loaded on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific, #NP0321), running at 150-180 V for 60-120 min in 1x MOPS ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 13,500 rpm at 4°C for 10 min and the supernatant was denatured using LDS loading buffer and reducing agent (Thermo Fisher Scientific) at 95°C for 10 min and run under reducing conditions on a 3-8% Tris-Acetate precast gel for 135 min at 100 V in Tris-Acetate running buffer supplemented with antioxidant (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: Recombinant human β2m-MR1-K43A-Fc was produced in CHO-K1 cells as previously described (10) and 4 µg/mL were coated onto 96 wells plates (Nunc, Cat#439454) for 18 h at 4°C ...
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bioRxiv - Genetics 2022Quote: ... and boiled at 95°C for 10 minutes before the samples were loaded in the NuPAGE 4%–12% Bis-Tris Gels (Invitrogen, #NP0335BOX & #NP0336BOX) for further resolution and western blot analysis.
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bioRxiv - Genetics 2022Quote: ... and boiled at 95°C for 10 minutes before loading the samples in NuPAGE 4%–12% Bis-Tris Gels (Invitrogen, #NP0335BOX & #NP0336BOX) for further resolution and western blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of protein from both PNS and LM (10 μg/lane) was resolved by SDS-PAGE (Invitrogen NuPage gel 4–12%).
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and 150 mM NaCl and 10 µL of 2X loading dye and then ran on a NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen, #NP0335) and stained with InstantBlue® (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 10 μL was directly applied to glow-discharged Quantifoil R1/4 Au-SiO2 200 grids and incubated for 10 minutes in a Vitrobot Mark IV plunger (Thermo Fisher Scientific) set to 100% humidity and 22 °C prior to blotting and plunge-freezing ...
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bioRxiv - Physiology 2022Quote: ... the samples were spun down at 4°C at 5000 rpm for 10 minutes and the plasma collected into a non-stick tube (Ambion, Catalog # AM12300). Samples were stored at −20°C until assayed.
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bioRxiv - Molecular Biology 2022Quote: ... The eluted samples were heated at 95°C for 10 minutes with 1x reducing sample buffer and loaded into a 4%-20% tris-glycine gel (Thermo Fisher Scientific) at 30 µL/well ...
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bioRxiv - Microbiology 2022Quote: ... and intracellular markers were stained with monoclonal antibodies for 45 minutes at 4°C with FoxP3 (FJK-16S) and IL-10 (JES5-16E3) (All antibodies were from Thermo Fisher Scientific). Cells were resuspended in PBS with 2% NCS and 2 mM EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... The grids were then blotted for 2 or 4 seconds (blot force −10, humidity 70%) before plunge freezing by rapid immersion in liquid ethane using the Vitrobot (Thermo Fisher Scientific). Grids were subsequently clipped.
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bioRxiv - Cancer Biology 2024Quote: ... lysates were extracted with Laemelli sample buffer for 10 minutes at 75°C and lysates were run on 4% to 12% Bis-Tris gels (Invitrogen; Thermo Fisher Scientific). Proteins were transferred to nitrocellulose membranes (Prometheus ...
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bioRxiv - Bioengineering 2024Quote: ... followed by overnight dialysis against sterile PBS at 4°C in 10 kDa Slide-a-Lyzer G2 cassettes (Thermo Fisher, MA, USA). The batch size of liposomes was 1.2 L in total ...
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bioRxiv - Bioengineering 2024Quote: ... followed by overnight dialysis against sterile PBS at 4°C in 10 kDa Slide-a-Lyzer G2 cassettes (Thermo Fisher, MA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 80 °C for 10 min and proteins separated using precast NuPAGE 4–12 % Bis-Tris gels (Thermo Fisher Scientific, NP0322PK2) at 80 V for ca ...
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bioRxiv - Cell Biology 2023Quote: ... 50μg of protein per sample was separated on 4-20% or 10-20% gradient Novex Tris-glycine polyacrylamide gels (Thermo Fisher Scientific) transferred onto polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 21130 g for 10 min at 4°C and quantified for protein concentration using an albumin standard (Thermo Fisher 23209) and Bradford reagent (Bio-Rad 5000006) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5-10 µg of total protein was heated at 70°C for 10 min and then resolved on a 4–12% NuPAGE gel (Thermo Fisher Scientific) by SDS-PAGE ...