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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1:50 (v/v) B-27 supplement 50x (Gibco), 5 μg/ml insulin solution human (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and anti-b-tubulin (1/20000, #32-2600, Invitrogen) antibodies respectively ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% B-27 supplement (Thermo Fisher Scientific; 17504044). Cells were grown in neural base medium supplemented with SB-431542 (10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x B-27 supplement (Invitrogen, cat. no. 17504044), 1 mM N-Acetyl-L-cysteine (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Amphotericin B (1 ug/ml) (Thermo Fisher Scientific, #15290026).
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bioRxiv - Genomics 2023Quote: ... 1× B-27 Supplement minus vitamin A (Gibco, 12587010), GlutaMAX Supplement (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-tubulin (cat# MA5 16308, Invitrogen,1:2000) confirmed no cellular contaminates.
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bioRxiv - Biophysics 2023Quote: ... with the addition of B-27 (1:50) (GIBCO), N-2 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml B-27 Supplement Minus AO (50X) (Gibco), 20 mM potassium chloride (Carl Roth) ...
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bioRxiv - Cell Biology 2022Quote: ... with 9% FBS (ThermoFisher Scientific) and 50 U/ml penicillin ...
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bioRxiv - Genomics 2022Quote: ... we benchmarked 23 different human genotyping arrays including 14 arrays from Illumina and 9 arrays from Affymetrix. The examined arrays contain the numbers of tag SNPs (array size ...
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bioRxiv - Molecular Biology 2021Quote: Spodoptera frugiperda 9 (Sf9) (Invitrogen) and High Five™ insect cells (Expression Systems ...
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bioRxiv - Microbiology 2020Quote: ... 0.4 μM SYTO-9 (Invitrogen) and 6 U Bst3.0 DNA polymerase (NEB ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... pH 9 (Gibco 25080-094). Mice were ad lib fed and given an oral gavage of BT2 at 40 mg/kg gavage around 13:00 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sf9 (Spodoptera frugiperda-9; Invitrogen) and High Five (Trichoplusia ni ...
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bioRxiv - Microbiology 2024Quote: ... SYTOTM 9 (ThermoFisher Scientific, S34854). FMTM 1-43 (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... assessment of both live and dead cells on the N27 cell line was performed with a combination of 10 μM CellTracker green and 4 μM Propidium Iodide (PI, Invitrogen, ThermoFisher) solutions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotech) or the AttuneNxT acoustic focusing cytometer (4 lasers, 14 parameters, Thermo-Fisher Scientific). Data were processed using FlowJo software (ver.9.9 or 10.7 ...
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bioRxiv - Molecular Biology 2020Quote: GST or GST-PHF1-tudor proteins (10 μg each) were incubated with 4 μl (25% slurry) of Glutathione Magnetic Beads (88821; Thermo Scientific) in 250 μl of binding buffer (125 mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2020Quote: Fibrinogen-Alexa647 was obtained by reacting Fibrinogen (4 mg/ml in Fibrinogen buffer) with a 10-fold molar excess of NHS Alexa 647 (ThermoFisher, A20006) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific, #NP0321BOX). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and boiled at 95°C for 10 minutes before loading on to NuPAGE 4-12% Bis-Tris gels (Thermo Fisher).
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bioRxiv - Genetics 2019Quote: ... Lysate was then subjected to sonication for five minutes at 4°C (amplitude 20%, 10 sec pulses, 50 sec rest between pulses) using a sonicator (Fisher Scientific), and centrifuged at 21,000 x g for 15 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... of proteins (corresponding to around 10 pooled organs) were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies). Separated proteins were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... infected erythrocytes were released from the host cell by lysis with 0.03% saponin in cold PBS containing 2 g/L glucose and washed three times with PBS/glucose by centrifuging 7 min at 10,000 × g at 4°C and 10 μL were collected in the last wash to count parasites using a Countess cell counter (Thermo Fisher). Cell pellets were flash-frozen and stored at −80°C until the metabolite extraction which was performed by treating samples with 1 mL cold methanol and three consecutive extractions with 2 mL of hexane followed by pulse-vortex for 1 min and 10 min sonication in an ultrasonic bath ...
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bioRxiv - Microbiology 2019Quote: Protein analysis was performed on 4%/10% polyacrylamide gels under reducing conditions (molecular weight marker Cat. Nr. 26616, Thermo Scientific, Lithuania). Immunoblotting was done as described in [73] ...
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bioRxiv - Cancer Biology 2019Quote: ... and 3 days of incubation cells were fixed with 4% PFA in PBS and nuclei stained with 10 μM Hoechst 33342 (H1399; Thermo Fisher). For each well ...
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bioRxiv - Genetics 2020Quote: ... Samples were then heated at 70°C for 10 minutes before loading on a gradient-PAGE (4 % −12 %) Bis-Tris gel (Invitrogen #NW04125BOX). After their separation ...
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bioRxiv - Genomics 2020Quote: ... equal amounts of total protein (10 – 30 μg) were loaded per lane into 4-12% Bis-Tris Bolt gels (Invitrogen # NW04125BOX). Western transfer was performed using an Invitrogen iBlot system (# IB21001 ...
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bioRxiv - Bioengineering 2021Quote: ... These proteins were then reduced at 90°C for 10 min and loaded into a 4-12% bis-tris gel (Life Technologies). This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... The expression culture was finally centrifuged for 10 min at 4°C and 5316 × g (Thermo Scientific H-12000 BioProcessing Rotor). The cell pellet was separated from the supernatant and resuspended with 40 ml HEPES-buffer per 2 l culture on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Snap frozen muscles were sectioned into 10 μm thick slices and fixed for immunostaining in either acetone (Fisher Scientific, A16P-4), 4% PFA (Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were washed with PBS for 4×10 min and incubated with secondary antibodies (goat anti-rabbit Alexa488 (A11034, Thermo Fisher), goat anti-mouse Alexa555 (A21424 ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Molecular Biology 2021Quote: ... nuclear extract was dialyzed for four hours at 4°C in a 10 kD cut-off Slide-A-Lyzer MINI Dialysis Device (Thermo Scientific) in buffer containing 20 mM HEPES pH 7.9 ...
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bioRxiv - Microbiology 2020Quote: 10 μg per lysate or 10 μL of immunoprecipitated sample was resolved on a NuPAGE 4-12% Bis-Tris Protein Gel (Invitrogen, NP0336). Western blotting was performed using the primary antibodies anti-HA (Proteintech ...
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bioRxiv - Neuroscience 2021Quote: ... Protein samples (10 μg/well) were loaded to NuPAGE Novex 4–12% Bis-Tris protein gel (ThermoFisher Scientific, Carlsbad, CA, NP0336BOX) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed by perfusing 10% phosphate buffered formalin (Fisher, SF100-4) for 30 minutes followed by a 60-minute wash with dPBS (ThermoFisher, 14040133). The devices were stored at 4 degrees Celsius for not more than 2 weeks before further processing ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were fixed with 4% paraformaldehyde for 10 min at room temperature and mounted with Prolong Gold Antifade reagent mounting medium (Molecular Probes). Images were taken with a Leica TCS SP2 confocal microscope and processed using the FIJI software.
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bioRxiv - Cell Biology 2021Quote: The protein extracts were prepared with dissected fly tissues that were homogenized for 10 min at 4°C in 1x RIPA buffer containing protease inhibitors (ThermoFisher A32955), followed by centrifugation at 16,000 x g for 15min at 4°C to remove the debris ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were loaded on 10% polyacrylamide gels or were run on NuPage Novex 4–12% Bis-Tris gels (Life Technologies) and blotted onto nitrocellulose membranes using the iBlot device (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: The ubiquitylated yeast CMG was then incubated for 30 minutes at 4°C with 2.5 µl (per 10 µl ubiquitylation reaction mix) magnetic beads (Dynabeads M-270 Epoxy; Life Technologies, 14302D) that had been coupled to anti-FLAG M2 antibodies ...
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bioRxiv - Immunology 2022Quote: ... The cell lysate was heated to 95°C for 10 minutes and was resolved by SDS-PAGE using a 4-12% Bolt Bis-Tris gel (Thermo Fisher) and transferred to a nitrocellulose membrane (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... AML cells were grown in RPMI L-Glutamine (4 mM) supplemented or minimum essential medium (MEM)-α Glutamax supplemented with 10% foetal bovine serum (FBS, Invitrogen), 100 IU/mL penicillin and 100 µg/mL streptomycin (Life Technology ...
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bioRxiv - Immunology 2022Quote: ... cell lysates were centrifuged at 13,000g for 10 min at 4 °C and immunoprecipitated with Dynabeads MyOne Streptavidin C1 (Invitrogen, Carlsbad, CA). The immunoprecipitated samples were transferred onto PVDF membranes as described earlier and immunoblotted with HRP-conjugated anti-Fyn mAb (clone 15 ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM Bis-Tris pH 7.0) and resolved on a Novex NativePage 4–16% Bis-Tris Protein Gel (Invitrogen CAT# BN1004BOX) run at 150 V for 3 hours ...
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bioRxiv - Neuroscience 2020Quote: ... the mouse ventral midbrain samples were sonicated in 50 mM triethylammonium bicarbonate (TEAB)/4% SDS/10 mM dithiothreitol with Halt protease inhibitor cocktail (Thermo Scientific) for 5 min followed by heating at 95°C for 5 min ...