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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cells were washed thrice with sterile PBS and 200 μL of cell suspension were cytospined for 5 min (Shandon Cytospin 4, Thermo Scientific, Cheshire, UK). The identification of infected DPSCs was carried by Gimenez staining.
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bioRxiv - Immunology 2021Quote: ... Antibodies were harvested from cell supernatants at 4 to 5 days post transfection and purified using CaptureSelect™ LC-lambda (mouse) Affinity Matrix (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: 3D caecaloids grown in expansion medium for 4-5 days after passaging were dissociated into single cells by TrypLE Express (Gibco Thermo Fisher Scientific) digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... Dissociated tumor cells from Lcre;NICD1 animals treated with IMR-1 or vehicle were resuspended in 0.2 ml cold phosphate-buffered saline (PBS) and spun onto a slide at 800 g for 5 min using a Shandon Cytospin 4 Cytocentrifuge (Thermo Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stained for 15min at 4 °C in Flow buffer (PBS with 5% cosmic calf serum) containing the following antibodies: CD11b-PE (Invitrogen, 12-0112-82), CD45-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... at 300 x g for 5 min at 4°C and resuspended in 50 μl of unlabeled anti-FCGR3/CD16-FCGR2B/CD32 Ab (Invitrogen, 14-0161-86) 10 μg/ml in FACS-buffer (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... FUCCI positive cells were cultured and washed once with phosphate-buffered saline (PBS, 300 x g / 5 min) and fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured at 95°C for 5 minutes and run on a Bolt™ 4-12% Bis-Tris polyacrylamide gel (Thermo Scientific #NW04125BOX). Proteins were transferred to a nitrocellulose membrane (Invitrogen #PB3310 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol, 50 mM maltose) and were concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4 ml) (Fisher Scientific, EMD Millipore). Proteins were further purified by gel filtration chromatography (Superdex-200 ...
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bioRxiv - Systems Biology 2023Quote: Labeled blots were washed 4 x 5 min each then incubated in SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific™ #34577) at room temperature for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.005 % BPB) and incubated 5 min at 100 ºC followed by electrophoresis on Novex™ 4-20% Tris-Glycine gradient gels (Invitrogen, WXP42020BOX; WXP42026BOX). Proteins were transferred onto 0.2 µm nitrocellulose membranes (Pall Laboratory ...
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Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised focibioRxiv - Cell Biology 2022Quote: ... Cells were then washed with PBST 4 times for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI 220 (Invitrogen, Thermo Fisher Scientific). Cells were viewed on a Zeiss LSM 800 confocal microscope.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction tubes were centrifuged for 5 min at 4 °C and 23000 rpm (approx. 49000 x g, Heraeus Biofuge Stratos, rotor 3331, Thermo Scientific, Waltham, USA). In the next step ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL ribodepleted RNA was mixed with 4 μL 5× TGIRT buffer (2.25 M NaCl, 25 mM MgCl2, 100 mM Tris-HCl, pH 7.5; Ambion RNase-free Buffer Kit AM9010), and 1 μL random hexamer primers (xGen) ...
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bioRxiv - Plant Biology 2024Quote: ... annealing at 52ºC for 5 s and extension at 60ºC for 4 min using a GeneAmp® PCR System 9700 thermocycler (Applied Biosystems, Warrington, UK). Samples were Sanger-sequenced using a 3730 Series Genetic Analyzer Thermo Fisher Scientific UK Ltd (Loughborough) ...
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bioRxiv - Immunology 2020Quote: ... isolated B cells were stained with Cell Proliferation Dye eFluor™ 670 (5 μM, Invitrogen) and measured after 48 and 96 h.
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bioRxiv - Biophysics 2020Quote: ... and 5 mL of B-PER™ complete bacterial protein extraction reagent (Thermo Scientific #89821) with 2 mM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... from 5-30% solvent B (LC-MS grade 0.1% formic acid (#A117, Thermo Fisher Scientific) and acetonitrile ...
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bioRxiv - Immunology 2023Quote: ... B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies) supplemented with 10% FCS (Biowest) ...
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bioRxiv - Microbiology 2021Quote: ... and antibiotic/antimytotic cocktail (10 units/mL penicillin, 10 µg/mL streptomycin and 25 fg/mL Amphotericin B, Gibco). Ld652Y cells were originally derived from Lymantria dispar ovaries and have been found to be most sensitive to JcDV infection (18) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 mM 2-Mercaptoethanol (Gibco)) at 37 °C in a humidified incubator containing 5% CO2 ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μl ATP (10 mM, Thermofisher), 0.5 μl T4 DNA ligase (30 u/μl ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... MMP-2 and −9 activity was determined using gelatine containing zymography gels (Thermo Fisher Scientific, Karlsbad, CA, USA) as previously described (37).
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bioRxiv - Microbiology 2020Quote: Experiments to determine the sensitivity of the two RT-qPCR methods and nested PCR were completed using serial dilutions of each transcript (5*103 to 10−1 copies/5 µL) in a previously described RNA storage buffer containing RNA storage solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 1.9 µm beads-EV1106 column using an Evosep one HPLC system coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher, San José, CA, USA) and a 30 SPD preprogramed gradient.
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bioRxiv - Molecular Biology 2024Quote: ... 1.9 µm beads-EV1106 column using an Evosep one HPLC system coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher, San José, CA, USA) and a 30 SPD preprogramed gradient.
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bioRxiv - Genetics 2021Quote: ... followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher) following the manufacturers recommendations (Supplemental Tables 19 and 20).
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bioRxiv - Neuroscience 2024Quote: ... Cells were then blocked for one hour with 10% goat serum (Invitrogen, 16210072) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... one was subjected to 10 μl RNase A/T1 mix (ThermoFisher Scientific, EN0551), 10 μl lysis buffer was added to the control sample and both samples were incubated at 37 °C for 10 minutes ...
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bioRxiv - Physiology 2019Quote: ... or vehicle alone (DMSO) diluted in DMEM medium for 4h (37C°) and stained with LysoTracker DND-99 (Invitrogen). Cells were fixed in 4% PFA ...
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bioRxiv - Microbiology 2023Quote: ... mice were orally gavaged daily 4h into the light cycle with 100 μL of either vehicle (PBS, Gibco) or 108 CFU Limosilactobacillus reuteri ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated for 4h with goat anti-mouse secondary antibody conjugated to AF-594 (Cat # A-11032, Invitrogen) diluted 1:2000 in IP blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... in 2 ml of cell medium (10% hiFBS [Fisher] and 1% Pen/Strep [Invitrogen]). The plates were incubated overnight ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... The alkaline hydrolysis ladder was prepared by incubating 0.4 pmol 5’ end-32P-labeled RNA in 9 µL of 1x alkaline hydrolysis buffer (Ambion) for 5 min at 95°C ...
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bioRxiv - Microbiology 2024Quote: ... and cells were stained for 15 min with 5 μM green fluorescent nucleic acid stain SYTO™ 9 (Invitrogen) at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... blocked for 30 min with 1% BSA in PBS-Tween (0.1%) and then incubated for 1 h with the primary antibodies (the lysosomal marker LAMP-1 (Invitrogen, 4-1071-82, 1:500) or the microglia marker IBA1 (Wako ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
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bioRxiv - Immunology 2020Quote: Luciferase-based cytotoxicity assay was carried out for Her2 and CD19 CAR T cells as previously described (22) at a 10:1 ratio of effector to target cells of using Firefly Luc One-Step Glow assay (ThermoFisher #16197). For analysis of cytokine release ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of diluted RNA (1:10) was added to 20μL of Superscript III Platinum One-Step qRT-PCR mix (Invitrogen 11746-100) containing 12.5 μL reaction mix ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Neuroscience 2019Quote: ... cycling synaptic vesicles were stained with the styryl dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridium dibromide (FM1-43; Molecular Probes, Inc., Eugene, OR). For labeling of synaptic vesicles ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... using crude extracts from plant leaves and monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4-methylumbelliferyl-β-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from gastrocnemius muscles of experimental mice (n=4 BC-PDOX; n=4 BC-PDOX PIO; n=3 NSC-Con) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods21 ...
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bioRxiv - Cell Biology 2022Quote: ... primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008; 4 μg/ml) in 0.2% BSA for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quantity was measured using a Qubit 4 fluorometer (Qubit 4 fluorometer; Qubit dnDNA HS Assay Kit, Cat No. Q32854 Invitrogen, Eugene, OR, United States). RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 4 young (⩽32 years) and 4 older patient (⩾40 years) granulosa cell samples using TRIzol (Life Technologies, Carlsbad, CA, USA) as previously described.111,112 Further ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were postfixed in 4% paraformaldehyde overnight at 4 °C and cut into 50 µm sections on a cryostat microtome (Thermo Fisher, NX50, Waltham, MA). Mounted sections were imaged on a scanning confocal microscope (TCS SP5 ...
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bioRxiv - Physiology 2021Quote: ... 10 mM Tris (Fisher Scientific, BP152-5) pH 7.4 ...
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bioRxiv - Physiology 2021Quote: ... 5 % of 10 mM ATP (ThermoFisher Scientific), 5 % human serum (SigmaAldrich) ...