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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Neuroscience 2021Quote: ... 10-30ug of protein was incubated with 1x reducing agent and 1x LDS sample buffer at 70°C for 10 minutes before immediately being loaded onto a BOLT 4-16% Bis-Tris gel (ThermoFisher Scientific) in 1x MES buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Blood samples were immediately centrifugated for 10 min at 3,500 rpm and 4°C using a Multifuge 3S centrifuge (Fisher Scientific SAS). The resulting plasma was frozen at −20°C and sent to the pharmacology lab to be analyzed ...
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bioRxiv - Microbiology 2022Quote: ... Data were collected on a FEI Titan Krios microscope operating at 300 kV using a Falcon 4 direct electron detector with a Selectris imaging filter (10 eV slit width) (ThermoFisher Scientific) at the Astbury Biostructure Laboratory (Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were centrifuged (14.000x g, 10 min at 4°C) and soluble protein concentrations were determined by a Bradford assay (Thermo Fisher Scientific). For digestion ...
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bioRxiv - Genetics 2020Quote: ... Samples were heated at 70°C for 10 minutes for denaturation and loaded onto a precast 4-12% mini bis-tris gel (Thermo Fisher) using the XCell SureLock Mini-Cell system (Thermo Fisher) ...
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bioRxiv - Systems Biology 2021Quote: ... The final transformation mixture contained 10 mM LiAc (Lithium acetate dihydrate, 98%, extra pure, ACROS Organics™, CAS 6108-17-4), 50 µg ssDNA (UltraPure™ Salmon Sperm DNA Solution ...
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bioRxiv - Genetics 2020Quote: ... Cells were heated for 10 min at 95 °C and separated on a 4-12 % NuPAGE Bis –Tris precasted PAGE gel (Thermo Scientific). Samples were run at 180 V for 10 min and processed by in-gel digestion (78) ...
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bioRxiv - Immunology 2020Quote: Protein samples in reducing condition were mixed with loading buffer containing β-mercaptoethanol and heated at 96 °C for 10 minutes before loading on 4-12% Bis-Tris Plus gels (Invitrogen). Samples in non-reducing conditions were diluted in loading buffer without any other treatment ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were harvested by centrifugation at 5,000 x g for 10 min at 4°C in a Fiberlite F9 rotor (ThermoFisher, cat.# 13456093).
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... cells were fixed with 4% paraformaldehyde (PFA) pH 7.4 at room temperature for 10 min and washed 3 times in PBS (Gibco Life Technologies,10010) for 5 min each ...
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bioRxiv - Cell Biology 2020Quote: ... pelleted and lysed at 4 °C for 10 min and ultrasound at 20% amplitude in lysis buffer (Ripa buffer, Thermo scientific) containing 1% protease inhibitor cocktail (Complete Mini-Roche Molecular Biochemicals ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then fixed in 4 % PFA for 10 minutes and stained using Click-iT Edu Alexa Fluor 594 Imaging kit (Life Technologies) according to manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The lysate was clarified via centrifugation at 16,100 × g for 10 min at 4°C then total protein concentration was measured using a detergent-compatible Bradford assay (ThermoFisher 23246). Lysate was transferred to a 384-well plate (30 μL × 3 TR-FRET replicates ...
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bioRxiv - Biochemistry 2021Quote: ... denaturation at 95 °C for 10 min and gradually temperature decrease to 4 °C during 20 minutes in a thermal cycler (Applied Biosystems). Ribonucleoprotein complexes (RNP ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Neuroscience 2020Quote: ... was denatured for 10 minutes at 90°C and loaded onto a Bolt BT Plus 4-12% gel (Invitrogen, Carlsbad, CA). After electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... cells were sorted into PCR plates containing 4 μl/well of ice-cold 0.5X PBS supplemented with 10 mM DTT (Invitrogen, Cat#Y00147), 8 U RNasin Ribonuclease Inhibitor (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Soluble proteins were retrieved after centrifugation at 15000 g at 4°C for 10 minutes and quantified using the Coomassie protein assay (Thermo Scientific). 25 µg of proteins were separated on a NuPAGE 4% -12% gel (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... sections were incubated with anti-EP3R rabbit antibody and anti-Fos goat antibody overnight at 4°C and then with Alexa594-conjugated donkey antibody to goat IgG (10 μg/ml; A11058, Thermo Fisher) and biotinylated donkey antibody to rabbit IgG (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... 1 × 108 cells in induced culture were collected by centrifugation at 1800 xg for 10 minutes at 4°C (TX-750 rotor, Thermo Scientific). Monoclonal α-GPEET and α-EP IgG (Cedarlane Labs ...
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bioRxiv - Microbiology 2022Quote: ... and heated at 70°C for 10 min before being loaded onto NuPAGE 4 to 12% bis-Tris protein gels (Life Technologies). The proteins were transferred using iBlot™ 2 Transfer Stacks (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 21,000 x g for 10 minutes at 4°C and protein concentration was determined by Bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Proteins were reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Cancer Biology 2024Quote: 5000 human and murine HSPCs/HSCs were fixed with 4% paraformaldehyde (PFA) for 10 min at RT and centrifuged (Tharmac) on poly-L-Lysine slides (Fisher Scientific). Immunofluorescence was performed as described previously (11,14) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation at 4°C with either 10 μl of pre-equilibrated protein A or G Ultralink beads (Thermo Scientific). The resin was washed several times with immunoprecipitation buffer spinning at 700 g for 30 sec ...
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bioRxiv - Neuroscience 2022Quote: ... and then the eluate was dialyzed overnight at 4°C in PBS using a 10 kDa dialysis membrane (Thermo Fisher Scientific). The concentration of IgG present after dialysis was determined using a BCA assay and adjusted by dilution with PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Immunology 2022Quote: Cells were seeded in a 24-well plate on sterile 13 mm coverslips and fixed for 10 minutes with cold 4% w/v formaldehyde (Fisher Scientific) and cold 8% w/v formaldehyde in HEPES buffer ...
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bioRxiv - Neuroscience 2024Quote: BV-2 cells were fixed with 4% PFA for 10 minutes and subsequently incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... The lentiviral construct of interest was co-transfected with the packaging constructs pCMV-VSV-G and pCMV Gag/Pol at a ratio of 10 µg: 4 µg: 6 µg respectively using Lipofectamine 2000 (60 µL, Invitrogen 11668027). After 4 h ...
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bioRxiv - Microbiology 2024Quote: His-pulldowns and OD600-matched periplasmic extracts were mixed with LDS sample buffer and denatured at 95°C for 10 min before being separated on 4-12 % bis-tris gels in MOPS or MES buffer (Invitrogen, USA). Gels were then electroblotted onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then denatured at 95°C for 5 min before loading 10 μg of samples onto a SDS-PAGE gel (Novex 4%–20% Tris-Glycine Mini Gels, cat#XP04200BOX, Thermo Scientific). Proteins were transferred to a PVDF membrane 0.2 μm (cat#10600021 ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 13,000 rpm for 10 min at 4°C and the protein concentrations were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... The soluble fractions of cell lysates were isolated by centrifugation at 20,000 g at 4 °C for 10 min and quantified by a BCA kit (Thermo Fisher Scientific). Protein samples were denatured with the addition of 6 × SDS sampling buffer and incubated at 95 °C for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed three times with PBS or PBST for 10 min and incubated with secondary antibody anti-mouse (4 μg/ml, Thermofisher #A11030) or anti-rabbit (4 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were heated at 70°C for 10 minutes and separated on 4-12% gradient Bis-Tris SDSPAGE gels (Invitrogen, NP0323) and transferred to 0.2μm nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Total lysates (10-20 µg) were separated by SDS-PAGE on NuPAGE™ 4-12% Bis-Tris Midi Protein Gels (Invitrogen) or 3-8% Tris-Acetate CriterionTM XT Precast Gels (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... NuPAGE® LDS Sample Buffer (4X) and NuPAGE®Novex 4-12% Bis-Tris Protein Gels (1.0 mm 10 well) were obtained from Life Technologies. Quick StartTM Bradford 1x Dye Reagent was from Bio-Raid ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Cells were washed three times with RNase-free DPBS and fixed for 10 min in paraformaldehyde lysine phosphate (PLP) buffer (pH7.4) containing 4% paraformaldehyde (PFA) (28908, Thermo Fisher Scientific), 5.4% glucose and 0.01 M sodium metaperiodate ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cell lysates were cleared by two consecutive centrifugation steps at 3000 rpm and 12000 rpm at 4°C for 10 min each (Heraeus Multifuge X3R, TX-1000 rotor, Thermo Scientific). After clearance 0.25% “NP-40 alternative” (Milipore 492016 ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... The metaphases were fixed with 4% formaldehyde for 10 min at RT and finalized with DAPI ProLong Gold Antifade Reagent (Thermo Scientific). For quantification of TRF2/RAP1 abundance on metaphase chromosomes in U2OS WT and ZNF524 KO clones ...
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bioRxiv - Microbiology 2023Quote: ... Lysates from the secretomes and intracellular fractions were centrifuged (10 min, 14,000 g, 4 °C) and soluble protein concentrations were determined (Bradford assay, Thermo Fisher Scientific). For digestion ...
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bioRxiv - Biophysics 2023Quote: ... The mixture of cleavage products was dialyzed overnight at 4°C using a Slide-A-Lyzer cassette (10 kDa cutoff, Thermo Scientific) in 4 l dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to electrophoresis in 10% or 4–12% Bis-Tris gels and transferred to nitrocellulose membranes using iBlot2 (Life technologies). Membranes were probed for VEGF-C followed by horseradish peroxidase-conjugated secondary antibodies (Thermo Fischer Scientific) ...