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Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... we seeded HEK 293 cells in a 4-chamber glass bottom dish (D35C4-20-1-N, Invitrogen). When the cells covered 70-80% of the chamber of a dish (almost 24 hours after seeding) ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples were mixed in a 1:4 ratio with 4X Native-PAGE sample buffer (ThermoFisher Scientific) and loaded into NativePAGE™ 3–12% Bis-Tris protein gels (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... PVDF membrane was incubated at 4 °C with 1:1000 ratio of PIKFYVE antibody (Invitrogen, PA5-13977) or 1:1000 ratio of α/β-Tubulin antibody (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated overnight at 4° with primary antibodies diluted 1:200 in PBS (clone 302, ThermoFisher). LSAB and AEC Kits (DakoCytomation ...
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bioRxiv - Genetics 2020Quote: ... 400 μl of lysate was treated with 4 μl of DNase I (1 U/μl, Thermo Scientific) and 8 μl of RNase I (100 U/μl ...
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bioRxiv - Neuroscience 2022Quote: ... 4 dpf zebrafish larvae were exposed during 1 hour to LysoTracker Red DND-99 (Ref.L7528, Invitrogen/ThermoFisher) diluted at 5 μM in fish system water supplemented with methylene blue and PTU ...
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bioRxiv - Neuroscience 2022Quote: ... 4 dpf zebrafish larvae were exposed during 1 hour to LysoTracker Red DND-99 (Ref.L7528, Invitrogen/ThermoFisher) diluted at 5 μM in fish system water supplemented with methylene blue and PTU ...
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bioRxiv - Biochemistry 2022Quote: ... and maintained in Low Glucose DMEM (5.5 mM glucose, 1 mM pyruvate, 4 mM glutamine; #31600034, Gibco) supplemented with 10% fetal bovine serum (#12605729 ...
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bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: THP-1 cells were differentiated into 4-well Nunc Lab-Tek II Chamber slides (ThermoFisher, Waltham, MA) at a density of 105 cells per well and differentiated as described above ...
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bioRxiv - Microbiology 2022Quote: Spn serotype 4 strain TIGR4 was grown overnight on 1% agar- Todd Hewitt Broth (Thermo Fisher Scientific) with yeast (THY ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were electroporated in the MES buffer in 1 mm 4%-12% Bis-Tris gels (Invitrogen) and blotted onto PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with primary antibodies overnight at 4 °C: pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:1000 dilution), STAT1 (Santa Cruz Biotechnology SC-417 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were probed with an SLC35A2 polyclonal antibody overnight at 4°C (1:2000, Invitrogen, PA5-77178), followed by incubation with goat anti-rabbit HRP-linked secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained using primary antibodies overnight at 4°C: tubulin (Invitrogen MA1-80017, 1:1000), aSyn (BD Transduction Laboratories 610786 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated at 4°C for 30 min with antibodies against CD45 (Invitrogen, #MCD4528, 1/25), CD31 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... immortalized FSHD myoblasts were cultured in LHCN medium (4:1 DMEM:Medium 199 (ThermoFisher Scientific, Waltham, MA, USA) supplemented with 15% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... washed (4 x 300 µL PBST) and blocked for 1h at RT with 1% BSA (Fisher Scientific) in PBS solution ...
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bioRxiv - Bioengineering 2023Quote: ... constructs were fixed with 4% paraformaldehyde and washed with PBS and stained for LipidTox (Invitrogen 1:500) and DAPI (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Cancer Biology 2024Quote: ... Glut-1 and catalase proteins were separated on a 4% to 20% Tris-Glycine gel (Life Technologies) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted one last time at 400 x g for 5 min and resuspended into 5 ml of PBS (GIBCO) supplemented with protease inhibitors (ThermoFischer Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% v/v fetal bovine serum (FBS) (Vistech, SE100-B) and 1% v/v penicillin/streptomycin (Gibco, Cat# 15140122) at 37°C in a 5% CO2 incubator ...
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bioRxiv - Genetics 2023Quote: Patient derived immortalized lymphoblastoid cell lines were cultured in RPMI-1640 media (ATCC) supplemented with 10% FBS (ATCC) and 1% penicillin/streptomycin/amphotericin B (Thermo Fisher). Cells were maintained at 37°C in 5% CO2 ...
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bioRxiv - Genetics 2021Quote: ... the 700 bp PCR products were subcloned into the pCR™4-TOPO® TA vector and transfected into Escherichia coli DH5α cells (TOPO 4 Kit, ThermoFisher Scientific) followed by plasmid preparation and direct Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... pre-stained cells were loaded with Fluo-4 via by mixing with equal volumes of 2X Fluo-4 Direct™ loading solution (Fluo-4 Direct™ Calcium Assay Kit, Thermo Fisher). After a 30 min incubation at 37°C and then 30 mins at RT ...
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bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).
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bioRxiv - Genetics 2022Quote: ... The styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64, 514/670 nm absorption/emission Invitrogen™, Waltham, Massachusetts) was used at a final concentration of 16.5 μM in ddH2O from a 16.5 mM stock solution in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were quickly fixed overnight (ON) in 1x PBS pH7.4, 4% sucrose (Serva, Heidelberg, Germany) and 4% PFA (Paraformaldehyde; Thermo Fisher Scientific, Waltham, USA). Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... cells were centrifuged (300rcf, 7min, 4°C) and fixed (1h, 4°C) using the eBioscience Foxp3/Transcription factor staining buffer set (ThermoFisher #00-5523-00) fixation/permeabilization reagent ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, catalogue no. NW04122BOX) and transferred onto a polyvinyl difluoride membrane (Immobilon-FL ...
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bioRxiv - Bioengineering 2023Quote: ... The staining of MyHC was performed by incubating the samples for 4 hours with the mouse anti-MyHC antibody (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™), which was used at a 1:10 dilution for a final concentration of 10 μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... The grids were automatically blotted for 4 s at 4°C and 100% humidity and flash-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). Cryo-EM data were collected using a 300 kV Titan Krios G2 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... A droplet of 2.5 μL of purified NaV1.6EM complex was applied to the grid followed by blotting for 4-5s at 4°C under 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). In the case of the preparation of NaV1.6EM complex with 4,9-anhydro-TTX ...
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bioRxiv - Biophysics 2023Quote: ... cells were transfected with 4 μg of plasmid per 4 million cells using the lipofectamine 2000 reagent and protocol (Thermo Fisher Cat# 11668019). Nuclear extraction was performed 24 hours after transfection utilizing nuclear extraction kit from Abcam (ab113474) ...
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bioRxiv - Cell Biology 2024Quote: ... fixed in 4% PFA for 15 minutes at 4 °C and incubated in 1X InVitrogen eBioscience Cell Permeabilization buffer (InVitrogen 00-8333-56) for 15 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and coverslips were washed with 1X PBS and fixed with cold 4% PFA/PBS pH 7.4 (Paraformaldehyde Solution, 4% in PBS, Thermo Scientific™, #J19943.K2) for 15 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1:1 Supplement B-27 Minus Vitamine A (Gibco,12587-010), filtered with a 0.45 μm filter (VWR ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μg.mL-1 streptomycin and 1.25 μg.mL-1 amphotericin B (Life Technologies) and maintained in culture at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...