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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were added for 3 h before 45 min incubation with protein A or G beads (Dynabeads 10002D, 10003D from Thermofisher scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 40 whole embryos at stage 3 (18 h AEL) were homogenized using disposable pellet pestles (12-141-368; Fisher Scientific) and 1.5 mL Eppendorf tubes (0030125150 ...
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bioRxiv - Biochemistry 2019Quote: ... kinase assays were performed with shaking at 30 °C for 3 h with concentrations as indicated of 970-end length LRRK2 WT or G2019S (PV4873 and PV4882 respectively, ThermoFisher) or GST-MST3 (supplied by MRC Reagents and Services ...
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bioRxiv - Developmental Biology 2020Quote: ... the epithelial sheets were blocked in blocking buffer for 3 h at room temperature and incubated with the Alexa Flour 488 Click-iT EdU Imaging Kit (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The gel was run at 100V for almost 3 h then electro-transferred to nitrocellulose membranes using the iBLOT2 system (Invitrogen), and blocked in 5% nonfat milk solution ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times and stained with a goat anti-hamster IgG (H+L) Alexa Fluor 488 (ThermoFisher Scientific). Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII analytic cytometer (BD Bioscience).
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×105 CAR or NT-T cells were incubated with SSEA-4 and SSEA-3 (#GLY131 and #GLY122, respectively, Elicityl-Oligotech) at 200 μM for 48 h in 24 well plate (Nunc). 5×105 CAR and NT-T cells CFSE-labeled were incubated with or without 1×106 OVCAR4 and Caov-3 cells for 48 h in RPMI-10% FBS-Pen/Strep and L-Glut medium in 10 cm cell culture dishes (Nunc) ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was performed by using a gene specific primer (1 μM final concentration) located near the 3’ end of the FLEXI intron with 100 U Maxima H reverse transcriptase (ThermoFisher) in the manufacturer’s buffer and incubated at 25°C for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Then the co-cultures were washed for 3 h with 0.05% Tween-20 in PBS and incubated with fluorophore-conjugated secondary antibodies (ThermoFisher Scientific) and DAPI (1 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Infected MEFs were maintained in induction medium composed of DMEM/High Glucose:Medium 199 (4:1, HyClone:Gibco), 10% FBS (Gemini) ...
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bioRxiv - Biophysics 2020Quote: KPL-4 cells were transfected with the GV gene constructs and 1 µg/ml puromycin (Invitrogen) was added to cell cultures to select only the transfected cells ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 micron thick sections were used for IHC staining for CD8 (1:1000, #MA5-13263, Invitrogen) and FoxP3 (1:400 ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were generally run in 1 mm polyacrylamide 4-20 % Novex MES/SDS gels (Thermo Scientific) and using a Mini Gel Tank ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C followed by appropriate secondary antibody incubation for 1-2h (Thermo Fisher Scientific). IF samples were imaged using Zeiss LSM 710 confocal microscope ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin immunocomplexes were precipitated for 1 hour at 4°C with blocked Dynabeads Protein G (Invitrogen), subjected to multiple wash steps and the chromatin eluted in elution buffer (50 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Cancer Biology 2021Quote: ... with a ratio of 1 μg DNA and 4 μl transfection reagent diluted in OptiMEM (Gibco). Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 1 µM calcium-sensitive fluorescent dye (Fluo-4 AM; Invitrogen, Life Technologies) diluted in fresh Cl- buffer (154.6 mM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 1 µM calcium-sensitive fluorescent dye (Fluo-4 AM; Invitrogen, Life Technologies) diluted in fresh Cl- buffer (154.6 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Remaining supernatant was incubated at 4°C for 1 hour with Sepharose-G beads (Fisher Scientific), previously washed with 5% BSA in TBS-Triton buffer and preincubated overnight with 2.5 μg anti-GFP antibody ab290 (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... cultured for 4 days and directly stained with Hoechst 33342 (1 mg/mL) (Thermofisher Scientific, 62249) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... at 1:300 dilution overnight at 4°C and AlexaFluor546 (A546) anti-rat secondary antibody (Invitrogen) at 1:400 dilution for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with rabbit anti-GFP (1:1000; Invitrogen, A11122), mouse anti-S100β (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at cell yield from 1 brain per 4 transwell inserts pre-coated with 0.1% gelatin (Gibco), or in 12-well culture plates and monitored for confluence and polarisation ...
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bioRxiv - Genomics 2021Quote: ... enzymatic dissociation was performed for 4–6 minutes at 37°C in 1 mL TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 mL of TRIzol (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... We resuspended the tubulointerstitial single cells with 1 mL 4% neutral buffered formalin (Thermo Fisher Scientific) and fix for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26 ...
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bioRxiv - Bioengineering 2022Quote: ... and sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC) were purchased from Thermo Scientific (USA). Human BDNF ...
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A stable reference human transcriptome and proteome as a standard for reproducible omics experimentsbioRxiv - Cell Biology 2022Quote: ... the C18 columns were condition with 1 mL acetonitrile (ACN) (Thermo Scientific™, A955-4, USA), then equilibrated with 1 mL 5% ACN with 0.5% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated 2hrs at 4°C with secondary antibody (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Microbiology 2022Quote: ... Samples were also spread on agar plates supplemented with 4 µg mL-1 rifampicin (Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with isolectin GS-IB4 (1:100; Invitrogen, Cat# I32450) in PBS with 1% Triton X-100 ...
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bioRxiv - Cell Biology 2022Quote: ... VOs we’re fixed for 1 hour in 4% paraformaldehyde solution (Thermo Fisher Scientific, 7732-18-5) in PBS and for 2 hours in 2.5% glutaraldehyde in PHEM buffer (TAAB ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies (anti-GFAP 1:1000, Invitrogen #PA1-10004 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were incubated overnight at 4°C using TM4SF1 (ThermoFisher Scientific #PA5-21119, 1:200) and CD31 (Dako #M0823 ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was pre-cleared for 1 hour at 4°C with protein A Dynabeads (Invitrogen). Chromatin samples were incubated overnight at a low temperature with 10 µg ChIP-grade anti-RNA polymerase II (ab26721 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were fixed in 4% PFA and Alexa488-coupled streptavidin (1:10,000 in TBS, Molecular Probes) or Alexa647-coupled streptavidin (1:10,000 in TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... enzymatic dissociation was performed for 4–6 min at 37 °C in 1 ml TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 ml of TRIzol (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Sections were briefly fixed with 95% ethanol for 1 min followed by 4% paraformaldehyde (Thermo Scientific) for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated overnight at 4°C with 1:50 SMARCC1 primary antibody (Invitrogen PA5-30174). Samples were then incubated with 1:100 donkey anti-rabbit (Jackson ImmunoResearch 711-035-152 ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were run 1 cm into a 4-12% NuPAGE BisTris gradient gel (Life Technologies). The proteins were stained using colloidal Coomassie Brilliant Blue and the gel piece containing the proteins was cut out ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs (1 × 10^4/well) were dissociated into single cells with accutase (Thermo Scientific, MA, USA) after exposure to the rock inhibitor (Y-27632 ...
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bioRxiv - Synthetic Biology 2023Quote: ... before adding the HRP conjugated secondary antibody 1:10000 in 4% milk in PBS-T (Invitrogen) for 1 hour ...
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bioRxiv - Immunology 2023Quote: AML and MDM cells were fixed with 4% formaldehyde and 1% glutaraldehyde in phosphate buffer (Invitrogen) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or Caspase-1 over-night 4°C and with fluorochrome-conjugated secondary antibody (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked in 1% BSA/0.1% saponin-PBS with 4% horse serum (26050-070, Gibco) for 1h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... and guide RNA in a molar ratio of 1:4 was resuspended in Buffer R (Invitrogen) and left to stand at room temperature for 10min ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation overnight at 4°C with secondary antibodies (1:1000, Alexa-Fluor conjugated, Invitrogen). Following incubation in secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... at 1% B with Solvent A as 0.1% formic acid in water (Thermofisher Optima LS118-4) and Solvent B as 0.1% formic acid in acetonitrile (Thermofisher Optima LS120-4) ...