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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and 7.5□g of functional grade anti-IL-2 antibody (JES6-1, Thermo Fisher) were incubated together at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... The DNA/Fugene mix (ratio 1:2) was prepared in Optimem medium (Life Technologies). N2A cells were co-transfected with 1.5 μg of pCAGGs-Cas9(BB)-2A-GFP-gRNA #(n ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was mixed with 1 µl of RNase I (Affymetrix; 2 mg/ml) and SDS sample buffer and incubated at 30°C for 15 min before loading on a 10% SDS/polyacrylamide gel ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were serum-starved for 1 hour in 2 mL of Opti-MEM (Gibco). Cells were subsequently incubated with ligand and test compounds (1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue sections were immunostained with primary antibodies: E-cadherin (1:100; Invitrogen, ECCD-2), cytokeratin-8 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... in 2 ml of cell medium (10% hiFBS [Fisher] and 1% Pen/Strep [Invitrogen]). The plates were incubated overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Culture medium consisted of 2:1 mixture of DMEM/F12 media (Thermo Fisher Scientific) supplemented with 2 mM GlutaMAX (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... of the bacterium (1 µm, 2 µm polymer beads 4010A, 4202A, ThermoFisher Scientific, UK). The particles were diluted to 2.1 × 10−3 g ml-1 before transfer to glass cuvette with or without transparent soil ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 2% FBS in HBSS containing 1 μg/mL DAPI (Invitrogen, cat# D1306), spun down ...
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bioRxiv - Microbiology 2021Quote: ... 1,000 μ g ml−1 streptomycin and 2 mM glutamine (RPMI only) (Life Technologies). Cells were checked permanently according to morphology and functional features (SAMHD1 expression for THP-1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated at room temperature for 1-2 minutes with GMP EDTA (15575020, Invitrogen). After aspiration of EDTA ...
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bioRxiv - Biophysics 2020Quote: ... (2) Cells were treated with Ca2+-free 1× PBS (DPBS, Catalog No.14190144, Gibco). (3 ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% mercapto-2-ethanol and 1X Halt Protease Inhibitor Cocktail (Thermo Scientific; 1:200). Proteins were separated using SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed by adding 1 mL of 2% Formaldehyde (Fisher Scientific BP531-500) onto the overlay medium for at least 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Rat monoclonal anti tyrosinated tubulin YL1/2 was diluted 1:500 (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in PBS containing 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, Molecular Probes) to stain DNA ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal anti-HA (clone 2-2.2.14) DyLight 650 (1:1000; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... stained with 40,6-diamidino-2-phenylindole (DAPI, 1:1000 in PBS, 20 min, ThermoFisher) to label cell nuclei and mounted with Prolong diamond antifade reagent mountant (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μL of a 1:1000 dilution of ERCC RNA controls (Thermo Fisher 4456740) were spiked into each sample ...
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bioRxiv - Cancer Biology 2020Quote: ... For mitochondrial membrane potential cells were stained with 2 μM JC-1 (Life Technologies) for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and then washed with PBS and then passaged 1:2 with StemPro Accutase (Invitrogen) for 5 minutes at 37 °C and replated onto plates which were coated with hESC qualified matrigel (1 hour at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... including rabbit anti-SARS-CoV-2 Spike (S1/S2) polyclonal antibody (1:2,000; Invitrogen) and mouse anti-β-actin monoclonal antibody (1:5,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were counter-stained with 4,6-diamidino-2-phenylindole (DAPI, Molecular Probes, 1:5000) and mounted using Prolong Gold Antifade reagent (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... cells were transfected with Lipofectamine 2000 at a 2:1 ratio (Invitrogen, Carlsbad, CA) immediately after plating cells ...
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bioRxiv - Developmental Biology 2019Quote: ... Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2 ...
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bioRxiv - Neuroscience 2021Quote: ... using 1 µg of DNA and 2 µL of Lipofectamine 2000 (Thermo Fisher Scientific) per well of a 24-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... 2×106 cells were incubated with Hoechst 33342 (1 mg/mL) (Thermofisher Scientific, 62249) to stain the nuclei ...
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bioRxiv - Microbiology 2021Quote: ... 2 µl of 1 % GFP-non-toxic fluorescent cholera toxin (Thermofisher, catalogue number C34775) was injected as described previously 36,37 into three sites in the duodenal wall ...
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bioRxiv - Immunology 2021Quote: ... The following day 300μL of ExpiFectamine Enhancer 1 and 3mL of Enhancer 2 (Invitrogen) was added and the secreted recombinant Spike or RBD protein in culture supernatant were harvested after 72 hours and affinity purified using HisTrap HP Column (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... followed by laminin (Thermo Fisher 23017015, 1-2hr at 37°C, 2 μL/well) overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.4) and then loaded with 1 μM Fura-2 AM ester (Life Technologies) in calcium buffer for 45 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies include Anti-beta tubulin (1:500, mouse monoclonal, 2 28 33, Invitrogen), Anti-Hec1 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged 1-2 times per week after dissociation with TryplE (Gibco, 12605010) or EDTA in DPBS (final concentration 0.5mM ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM l-glutamine and 50 μg ml−1 of penicillin and streptomycin (Invitrogen). Cells were incubated at 37 °C in 5% CO ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated 2:1 beads to cell with CD3/28 (Dynabeads, Life Technologies) for 72h then harvested ...
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bioRxiv - Immunology 2021Quote: ... RNA samples were treated with 1 µl DNase I (2 U) (Ambion™, #AM2222) per 10 μg of total RNA in a 50 μl reaction for 30 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: 1×106 LDN or NDN were suspended in PBS-/- + 2% fetal calf serum (Gibco) and stained with CD66b ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with monoclonal HA Tag antibody (1:2000 dilution, Invitrogen, 2-2.2.14,), incubated with anti-mouse IgG-horseradish peroxidase (IgG-HRP ...
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bioRxiv - Microbiology 2022Quote: ... stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... 1-2×104 cells were lysed in 200 µL of TRIzol (Thermo Fisher Scientific) and RNA was isolated according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg mL-1 10,000 MW FITC lysine charged dextran (ThermoFisher Scientific, Waltham, MA) and 50 ng µL-1 of Renilla Luciferase (RLuc ...
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bioRxiv - Cell Biology 2022Quote: ... 1-2 µg of total plasmid DNA was transfected using lipofectamine 3000 (Thermo Scientific). Cells were trypsinized and replated onto No 1.5 coverglass the following day and analyzed ∼12-16 h later by indirect immunofluorescence ...
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bioRxiv - Neuroscience 2022Quote: ... then a fluorescent Nissl stain was applied (1:200 in 2% NDST, Thermo Fisher N21479 for MORmCh and KORtdTomato or N21483 for DORGFP and NOPYFP ...
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bioRxiv - Immunology 2022Quote: ... then with 2 μl of 20 mg ml−1 proteinase K (Thermo Fisher Scientific) for 1 h at 55° C ...
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bioRxiv - Immunology 2022Quote: ... Transfections were performed using with a 2:1 ratio of lipofectamine 2000 reagent (Invitrogen) to DNA.
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bioRxiv - Microbiology 2022Quote: ... mouse anti-claudin 1 monoclonal antibody (2 µg/ml, 37-4900, Thermo Fisher Scientific); rabbit anti-claudin 3 polyclonal antibody (1:100 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... 2) Concentrate worms by centrifugation (1 minute, 4000-RPM, Thermo Scientific Sorvall Legend X1R), discard the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... Mouse anti-E-cadherin (2 μg/ml, clone HECD-1; Invitrogen, Carlsbad, California, USA) and mouse anti-CX3CL1 (2 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... 1.5 mL of enhancer 1 and 0.15 mL of enhancer 2 (Expifectamine kit, Gibco) were added to the cells ...