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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion (Noordstra et al. ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated for 1 h at 25°C with Alexa Fluor 488 or 647-coupled anti-IgG antibodies (Thermo Fisher). Coverslips were mounted on glass slides in 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Genomics 2021Quote: ... The cells were washed and incubated with an Alexa Fluor 488-conjugated secondary antibody (10 μg/mL for 1 h at 37°C; Invitrogen, USA). The cells were finally stained with calcofluor white (5 μg/mL for 1 h at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... and then stained at RT for 1 h in 50 µL of the 2nd antibody diluent (Alexa Fluor 546 Goat Anti-mouse IgG, A-11030, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse IgG (H+L) (A-11001, 1:500; Molecular Probes), Alexa Fluor 555 goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was discarded into 2M NaOH and membrane was washed seven times with each 160 µL PBS and blocked 1 h with 160 µL Superblock (Thermo Scientific) prepared in MilliQ ...
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bioRxiv - Immunology 2021Quote: ... coated overnight at 4⍰°C with 2μg/well SARS-CoV-2 FL-S protein diluted in PBS were washed with PBS/Tween (0.05% v/v) and blocked for 1⍰h with 100⍰μl per well of Blocker Casein in PBS (Thermo Fisher Scientific) at 20⍰°C ...
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bioRxiv - Bioengineering 2019Quote: ... was added at a 1:200 dilution and incubated with cells for 1 h followed by a 20 min incubation with 5 μg/mL Hoechst 33342 (Invitrogen, H1399). HLMVECs were stained for actin with phalloidin conjugated with Alexa Fluor 488 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Conditioned medium was obtained by mixing 50% fresh DMEM supplemented with 10% FBS and 1% penicillin/streptomycin and 50% filtered medium obtained from U2OS cells grown for 48 h and supplemented with 1% L-glutamate (Life Technologies). After 10 to 14 days ...
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bioRxiv - Cell Biology 2020Quote: Total RNAs were extracted using Trizol reagent and 1 μg of RNA was reverse-transcribed with the RevertAid H Minus First Strand cDNA Synthesis kit (Life Technologies). qPCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were washed twice with PBS and labeled with a secondary antibody (Alexa Fluor 488 donkey anti-Goat IgG (H+L) (A11055, 1:200, Life Technologies) in PBS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in TBST buffer with 0.5% milk and goat α-rabbit IgG (H+L) HRP conjugated secondary antibody (1:10,000, ThermoFisher Scientific 31460) in TBST were used to detect PstP-strep ...
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bioRxiv - Bioengineering 2021Quote: ... Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Thermo Fisher Scientific, A32731, 1:1000), PNA (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... cells were incubated for 1 h at 37°C with secondary antibodies: Goat anti-Mouse Alexa Fluor 488 (ThermoFisher, A-11001) and Donkey anti-Rabbit Alexa Fluor 555 (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: 15 pmol of RNA/DNA were radioactively labeled for 1 h at 37 °C using 5 U T4 Polynucleotide Kinase (Thermo Fisher) and 10 µCi γ-32P ATP ...
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bioRxiv - Genomics 2020Quote: ... The cells were recovered for 48 h with no antibiotic followed by selection in puromycin (1 μg/mL) (Thermo Fisher, A1113803). Single colonies were manually picked and expanded in 96 well plates ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were reduced by incubation with 200 mM TCEP at 55 °C for 1 h and alkylated by incubation with 375 mM iodoacetamide (IAA) (Thermo Scientific) for 30 minutes while protected from light at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... After 2 washes cells were incubated for 1 hour with 0.5 μg/ml of Alexa Fluor 555 goat anti-mouse IgG (H+L) (ThermoFisher Scientific, #A21424). After 2 washes cells were resuspended in PBS and analyzed by flow cytometry using a FACS BD LSR-Fortessa.
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bioRxiv - Immunology 2020Quote: ... T cells were first given αCD3/αCD28 stimulation for 24 h in the form of CD3/CD28-coated Dynabeads (1 bead:100 cells; Gibco, #11161D). The beads were then removed with a magnet ...
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bioRxiv - Immunology 2020Quote: ... Secondary staining’s were performed for 1 h at room temperature in the dark with biotinylated goat anti-rabbit IgG AlexaFluor 405 (1:400; A-31556, Life Technologies) and goat anti-mouse IgG1 AlexaFluor 647 (1:800 ...
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bioRxiv - Biophysics 2021Quote: ... Cleavage reactions were incubated at 37 °C for 1 h and the reaction was stopped by the addition of 0.36 U Proteinase K (Thermo Fisher Scientific), followed by incubation at 55 °C for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... After washing cells were incubated with secondary antibody for 1 h at RT (Alexa Fluor 488 goat anti-rabbit, Invitrogen, R37116). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... cryo-ET data of nucleocapsids in situ at control and ~1 h of 0.5 mM As(III) stress were also collected with a Volta phase plate58 (VPP, Thermo Fisher Scientific) for the purpose of better visualization ...
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bioRxiv - Molecular Biology 2022Quote: ... C2C12 cells were plated onto 24-well Seahorse XF24 cell culture microplates at a 8,000 cell density and 24 h later co-transfected with either negative control siRNA (Negative Control No.1 siRNA, Life Technologies) with empty vector (pCMV6-Entry ...
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bioRxiv - Bioengineering 2022Quote: ... About 1 μg of RNA was used to synthesize cDNA with a RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Scientific). CDNA was diluted 50 times before use in Real-Time quantitative PCR (RT-qPCR) ...
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bioRxiv - Biochemistry 2022Quote: ... reverse transcription was performed for 1 μg of mRNA by using oligo-dTs and the Maxima H Minus Reverse Transcriptase (Thermo Scientific). Approximately 50 ng of cDNA were used to perform realtime qPCR with the Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Amplified products were treated with 0.4U/μl Dpnl for 1 h at 37°C prior to the PCR purification with the GeneJET PCR Purification Kit (Thermo Scientific). 100 ng of DNA products were blunt-end ligated in 20 μl reactions with 40U/μl T4 DNA ligase in 1× T4 DNA ligase buffer overnight at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of RNA was retrotranscribed into cDNA using Maxima H minus cDNA synthesis Master Mix with dsDNase kit (ThermoFisher Scientific). RTqPCR was performed using PowerUp™ SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 ug of DNase-free RNA was used to synthesize cDNA with a RevertAid H Minus kit (EPO452, Thermo Fisher Scientific). A qRT-PCR was performed with a CFX Connect Real Time PCR machine (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Following the last washing step the membrane was incubated for 1 h at room temperature with anti-mouse-HRP antibody (Thermo Scientific) diluted 1:10,000 in TBS-T with 5% powdered milk and finally washed 3x in TBS-T for 10 min before adding ECL substrate (Amersham ...
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bioRxiv - Microbiology 2022Quote: ... with 0.5% BSA and goat anti-rabbit IgG (H+L) horseradish peroxide (HRP)-conjugated secondary antibody (1:10,000, Thermo Fisher Scientific 31460) in TBST ...
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bioRxiv - Bioengineering 2024Quote: ... sections were stained for 1 h at room temperature in a humidified chamber with AlexaFluor-488-labeled donkey polyclonal anti-mouse IgG (1:500, Invitrogen, A21202) and AlexaFluor-555-labeled donkey polyclonal anti-rabbit IgG (1:500 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and then incubated with Cy3-conjugated affinity-purified goat anti-mouse IgG (H+L) polyclonal antibody (1:100; ThermoFisher, cat.no. A10521) and Alexa 488-conjugated phalloidin (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV derived from A549 cells were isolated from the conditioned medium by differential ultracentrifugation ...
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bioRxiv - Pathology 2024Quote: ... AlexaFluor 488 goat anti-mouse IgG (H+L) and Alexa Fluor 594 goat anti-mouse IgG (H+L) secondary antibodies were used at 1:500 dilution (ThermoFisher Scientific). DAPI (4’,6-diamidino-2-phenylindole ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated with secondary antibody 1:1,000 Alexa fluor 488 goat anti Rabbit IgG (H+L) (Invitrogen, cat# A-11008) 1 hr at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and incubated with secondary antibody 1:1,000 Alexa fluor 555 goat anti Rabbit IgG (H+L) (Life Technologies, cat#A-A21428), 1:1,000 Alexa fluor 568 goat anti Mouse IgG2b (H+L ...
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bioRxiv - Biochemistry 2024Quote: ... The FeS cluster was removed according to 74 by incubation of the protein with 200 µM H2O2 and 10 mM EDTA for 1 h at room temperature following filtration through a Zeba spin column (Thermo Scientific). The respective activity assays were performed in an anaerobic chamber (Coy Laboratory Products ...
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bioRxiv - Cell Biology 2023Quote: ... then with the following antibodies in 5% BSA for 1 h: donkey anti-rabbit IgG Alexa Fluor 488 (A21206, Thermo Fisher) and α-Bungarotoxin Alexa Fluor 594 (B13423 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... 1 µg of RNA per sample was used for cDNA synthesis with the RevertAid H Minus First Strand Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were synthesized from 1 µg of total RNAs using the RevertAid H Minus First Strand cDNA Synthesis Kit (Fisher Scientific) with oligo(dT ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were washed three times in 1X PBS and then incubated for 1 h at room temperature with a secondary antibody (Donkey anti-rabbit conjugated with Alexa 568, Thermo Fisher Scientific Cat# A10042 ...
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bioRxiv - Cell Biology 2023Quote: ... that had been coated overnight with 100 μg/ mL poly-D-lysine at 37 °C and for 1 h at 37 °C with 10 μg/mL laminin (Life Technologies). After 30 min ...
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bioRxiv - Immunology 2023Quote: ... in PBS for 1 h at 25°C followed by permeabilization in 5% BSA in the presence of 0.05% Tween-20 (Thermo Fisher Scientific) for 15 minutes at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with goat anti-rabbit IgG (H+L) HRP conjugated secondary antibody (1:5000) (Thermofisher Scientific, 31460) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Following repeated washing in PBS slices were incubated in donkey anti-goat IgG H&L (Invitrogen Alexa Fluor 488; 1:500) and donkey anti-rabbit IgG H&L (Alexa Fluor 568 ...
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bioRxiv - Physiology 2023Quote: ... cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; Thermo Fisher Scientific, USA). Overnight incubation with primary antibodies (anti-VPS36 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT10 (Thermo Scientific, #90110) or TMTpro 18-plex (Thermo Scientific ...