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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... was incubated for 1 h at RT and the signal was detected using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Embryos were then washed with PBST and blocked with 5% goat serum before addition of the secondary antibody (Goat anti-rabbit IgG H&L DyLight 650, 1:200, 84546 Invitrogen, USA) and incubated overnight at 4°C with gentle rocking ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated for 1 h with a secondary antibody (goat anti-rabbit IgG, Alexa Flour 488 conjugate, ThermoFisher Scientific/Invitrogen, R37116) and DRAQ5 (Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000 dilution).
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed with PBS to eliminate dead cells and then incubated for 1 h in 200 μL of HBSS (#14025092, Gibco, ThermoFisher) containing 100 μM of Resazurin (#B21187 ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies (1:1000) used were: goat anti-mouse IgG(H+L) Alexa Fluor 594 (Thermo Fisher Scientific, A-11005).
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of the plasmid was digested with KpnI and BamHI in a 20 μL reaction for 1 h using FastDigest enzymes (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was eluted with nuclease free water at 12000 x g for 2 min after which 1 µg of RNA was transcribed into cDNA using the RevertAid H Minus First Strand Kit (ThermoFisher Scientific) and random primers ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were washed twice with TBS and then incubated for 1 h at room temperature with secondary antibody anti-mouse Alexa-546 (Life Technologies). Slides were washed twice with TBS and incubated with DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion ...
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bioRxiv - Physiology 2019Quote: ... MNT-1 cells were transfected with Epac H187 and ~24 h after transfection cells were serum-starved in OPTI-MEM (ThermoFisher Scientific) for another ~24 h ...
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bioRxiv - Immunology 2019Quote: ... for 1 h at room temperature if needed and finally mounted with ProLong® Gold Anti-fade Reagent with DAPI (Invitrogen). Fluorescence images of sections were acquired with a LSM780 confocal laser-scanning microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature before mounting in anti-fade reagent with DAPI (Prolong Gold Anti-fade, Gibco, Gaithersburg, MD).
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bioRxiv - Biochemistry 2019Quote: ... the glass slide was incubated at RT for 1 h in secondary antibody conjugated with Alexa Fluor 488 (A11017; Life Technologies) and SYTOX Orange Nucleic Acid Stain (S-11368 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were incubated for 1 hour at room temperature in appropriate secondary antibodies: Goat anti-mouse IgG (H+L) HRP (ThermoFisher Scientific) or Goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... and donkey anti-mouse IgG (H+L, Highly Cross-Adsorbed, Alexa Fluor 488; 1:500, catalog: A-21202, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Samples were then rinsed three times with 0.05% Tween-20 in PBS (vol/vol) and incubated with the appropriate secondary antibodies for 1 h at room temperature and counterstained with DAPI (Life Technologies).
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bioRxiv - Neuroscience 2021Quote: ... at 4°C for 24 h and then with an Alexa Fluor® 488-conjugated goat anti-rabbit secondary antibody (1:500, A11034, Invitrogen) at room temperature for 2h ...
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bioRxiv - Microbiology 2020Quote: ... Infections of epithelial cells (A549 and NuLi-1) were performed two days after seeding with starvation for 16 h before infection using RPMI 1640 Medium (Gibco 21875) supplemented only with 10 mM HEPES.
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with an Alexa 594 Goat Anti-Rabbit IgG (H+L) (1:500) (Thermo Fisher Scientific, Waltham, MA, USA) and Alexa 488 Phalloidin (1:50 ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesised from 1-5 μg of template RNA using he Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... cushion at 20,000 x g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (Thermo Fisher Scientific). Viral stock titers were titered on MRC-5 (TB40/EFb-eGFP ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells was acquired using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated for 1 h at 37°C with the following secondary antibodies: anti-mouse Alexa 546 (Thermo Fisher), anti-rat Alexa 488 (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 1 h (at RT with gentle shaking) in a blocking solution containing 10% normal goat serum (16210064, Life Technologies), 0.4% Triton X-100 and 0.1% glycine in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 5 μM E64D) and incubated for 1 h in CCF2 solution (LB, CCF2-AM, Solution B [CCF2-AM kit K1032] Thermo Fisher) in the dark ...
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bioRxiv - Plant Biology 2021Quote: ... cDNAs were synthesized from 1 µg of total RNAs using oligo(dT) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Fisher Scientific). Quantitative PCR reactions were performed in a QuantStudio5 (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then labeled at room temperature for 1 h with Alexa Fluor 488–conjugated goat anti-mouse secondary antibody (Molecular Probes), 0.2% saponin ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were again washed with Blocking Buffer twice for 1 h and mounted on microscope slides using ProLong Glass (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with V5-tagged WNT11 and 24 h post transfection proteins were crosslinked for 30 min with 1 mM DSS (Thermo Fisher) in 1 ml PBS + 1mM MgCl2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was transferred to a 0.2 µm nitrocellulose membrane and blocked for 1 h at room temperature with SuperBlock™ blocking buffer (Thermo Scientific). Membranes were then washed four times in TBST buffer before incubation in a 1:5000 dilution of Monoclonal ANTI-FLAG® antibody (Monoclonal ANTI-FLAG® M2-Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 times for 5 min and incubated for 30 min with secondary antibodies diluted in PBT + 0.5% BSA: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 1 h at room temperature with goat anti-guinea pig Alexa Fluor 647 secondary antibody (#A21450, Life Technologies). Sections were then incubated in the Click-IT reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... the specimens were incubated for 1 hr at RT with anti-rabbit IgG(H+L)-Alexa 568-conjugated antibody (Donkey; Invitrogen, A10042), anti-mouse IgG2a-Alexa 488-conjugated antibody (Goat ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The secondary antibody was Alexa Fluor 549-conjugated goat anti-mouse IgG (H+L) (diluted 1:250 in PBST containing 5% goat serum, Invitrogen, USA) which was incubated overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 16 h and then the digest reaction was quenched with 1% formic acid (Fisher Scientific, Czech Republic). The resulting peptides were desalted using a C18 column (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... the coverslips were incubated at room temperature for 1 h with Alexa fluor 568- conjugated goat anti-rabbit (A11011, ThermoFisher Scientific) or anti-mouse (A11004 ...
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bioRxiv - Immunology 2020Quote: ... The solution was allowed to mix at room temperature for 1 h and then added to recently washed and dried magnetic protein G Dynabeads (Thermo Fisher). The mixture was incubated for 1.5 h and resin was separated from the supernatant magnetically ...
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bioRxiv - Microbiology 2021Quote: Complex trypsin-digested peptide mix was first separated by 1 h high performance liquid chromatography (HPLC) gradient using reverse phase C18 columns on an Dionex Ultimate 3000 UPLC (ThermoFisher Scientific) followed by a run on Q-Exactive (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the cell monolayer was washed with 1X PBS and fixed for 1 h at room temperature with 10% neutral buffered formalin (ThermoFisher Scientific). Following formalin removal ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were washed three times in PB 0,12 M under agitation at RT and incubated with goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A488 (1:800, Life Technologies) diluted in the blocking solution for 2H ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated for 1 h at room temperature with secondary biotinylated antibodies or with the ABC kit (Cat. no. 32020, Thermo Fisher). SuperSignal West Femto (Thermo Scientific Cat n ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed two times with PBS and incubated for 1 h with CellROX Deep Red Reagent (Invitrogen, Carlsbad, CA) prior to analysis by flow cytometry ...
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... subsequently with goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 488 conjugate 1:1000 (A11001, Thermo Fisher Scientific) to define CMs ...
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bioRxiv - Developmental Biology 2019Quote: ... Slides were then washed three times in PBS for 15 minutes each before a 1 h room temperature incubation in secondary antibodies (Life Technologies) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μg RNA was reverse transcribed for 1 h at 50°C using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies) with 1 μg random primers ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cells grown on poly-L-lysine–coated coverslips were incubated in 5% CO2 at 37°C for 1 h with 5 µM MitoTracker Red CMXRos (Molecular Probes) in DMEM ...
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bioRxiv - Microbiology 2019Quote: ... The plate was then incubated at 30°C and the OD600nm of the sample was monitored for every 1 h within 24h using MultiskanTM GO Microplate Spectrophotometer (Thermo Scientific). Each combination was performed in triplicate ...