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Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 3 % of homogenate was added to 1 ml of TRIzol Reagent (Fisher Scientific cat# 15-596-026) and RNA was isolated as per the user guide referenced above ...
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bioRxiv - Developmental Biology 2022Quote: ... 1μg total plasmid DNA (1:3 SAM to gRNA ratio) was diluted in Opti-MEM (Gibco #31985062) and mixed with p3000 reagent ...
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bioRxiv - Cell Biology 2022Quote: ... pDEST-AD-CYH2 (assay version 1) and pDEST-AD-AR68 (assay version 3) using Gateway Technology (Invitrogen). Attributes of these plasmids are summarized in Suppl ...
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bioRxiv - Molecular Biology 2024Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... 150ng of DNA was run on 3 lanes of a 1% eGel (Thermo Fisher Scientific, Waltham, MA) and size-selected into two populations ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
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bioRxiv - Molecular Biology 2024Quote: ... complexes (DNA to PEI MAX ratio, 1:3, w/w) formed in Opti-MEM (Thermo Fisher Scientific) via incubation for 15 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... cells were transfected with 1 µg of plasmid library using 3 µl Lipofectamine 3000 reagent (L3000008, Invitrogen, Carlsbad ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were incubated in Cyanine 3 (Cy3) goat anti-mouse (Thermo Fisher Scientific, A10521, 1:500) and Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Samples were individually mixed with an equal volume of 1 µg/ml of 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI; Invitrogen), incubated for 15 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS 3 times and incubated with 1:10,000 DAPI (Invitrogen, Cat#D1306) for 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg DNA and 3 μl transfection reagent were mixed in 100 μL Opti-MEM media (GIBCO) for 20min at room temperature before the mixture was added into each well ...
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bioRxiv - Genomics 2022Quote: ... The concentrate (about 300-450 µl) was mixed 1:3 with Trizol LS (ThermoFisher cat# 10296-010), and the RNA extracted using the DirectZol RNA kit (Zymo cat# R2052) ...
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bioRxiv - Immunology 2023Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2mg/ml proteinase-K (Invitrogen; 25530049) for 10 minutes at 55°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 1:3 (µg DNA: µg PEI) in 200 µl Opti-MEM (Gibco, 31985070). Media was replaced after 24h ...
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bioRxiv - Cell Biology 2024Quote: Cells were washed 2x with PBS and gently dissociated with 1:3 diluted Accutase (Gibco, A11105-01). Lifted cells were washed 2x with cold dPBS (HyClone ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated in Cyanine 3 (Cy3) goat anti-mouse (Thermo Fisher Scientific, A10521, 1:500) in PBS-Triton X 0.3% ...
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bioRxiv - Bioengineering 2024Quote: ... glass coverslips were submerged in piranha solution composed of 3:1 sulfuric acid (H2SO4, Fisher Scientific, A300) and 30 wt% hydrogen peroxide (H2O2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then incubated in the NGS for at least 4 hrs and incubated overnight at 4°C with secondary antibodies: goat anti-rabbit Alexa Fluor 488 (Invitrogen Cat# A11070; 1/400) and goat anti-mouse HRP-conjugated (Thermo Fisher Scientific Cat# G-21040 ...
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bioRxiv - Bioengineering 2023Quote: ... in 2.5 μl NuPAGE® LDS Sample Buffer (4×) and 1 μl NuPAGE® Sample Reducing Agent (10×)] were loaded in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific, USA). Samples were denatured at 95°C for 3 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and brain tissues were weighed and homogenized at a density of 140 mg of dry tissue per milliliter in ethyl acetate / isopropanol (4:1) using a Bead Mill 4 homogenizer (Thermo Fisher Scientific, Waltham MA) and 2-mL pre-filled polypropylene microtubes (2.8 mm ceramic beads ...
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bioRxiv - Neuroscience 2021Quote: ... Autophagy was activated by keeping cells in HBSS for 2 h prior to treatment (24020-091, Invitrogen) and blocked by addition of 3-MA (Sigma- Aldrich ...
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bioRxiv - Immunology 2019Quote: ... and then grown in the absence of IL-2 for 24 h in RPMI-1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 h incubation with a fluorescent secondary antibody (Thermo Fisher/Invitrogen, Alexa Fluor 488). DAPI was used to visualize cell nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 h incubation with a fluorescent secondary antibody (Thermo Fisher/Invitrogen, Alexa Fluor 488). DAPI was used to visualize cell nuclei ...
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bioRxiv - Biochemistry 2021Quote: ... puromycin selection was started 36 h post-infection (at the concentration of 2 μg/mL, Life Technologies) and was maintained thereafter.
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were washed away after 2 h and the remaining cells incubated in RPMI (Gibco) supplemented with 10 % heat-inactivated pooled human serum (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Autophagy was activated by keeping cells in HBSS for 2 h prior to treatment (24020-091, Invitrogen) and blocked by addition of 3-MA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
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bioRxiv - Neuroscience 2019Quote: ... followed by a 2-hour incubation with the HRP conjugated reagent (1:1 in TBST, HRP Polymer Conjugate Rabbit Primary, Life Technologies, LOT:1709065A) at RT on a shaker ...
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bioRxiv - Cell Biology 2021Quote: SK-MEL-2 cells from clone Ti13 (hCLTAEN-1 /hDNM2EN-1) were cultured in DMEM/F12 with GlutaMax™ supplement (10565-018, Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... We cultured the mouse embryonic stem cell (ESC) line in 2-inhibitor based medium (Advanced DMEM/F12:Neurobasal (1:1) medium (Thermo Fisher, 12634028, 10888022) supplemented with 2.5% ESC-grade Tet-negative fetal bovine serum (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488, Goat-anti-chicken, Invitrogen A11039, 1:500; Alexa Fluor 594 goat anti-rabbit, Invitrogen A11012, 1:500). A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: Expanded Tregs or Tconvs were activated with IL-2 (100 IU/mL) and 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads T-Expander) for 4 d ...
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bioRxiv - Molecular Biology 2022Quote: ... for 2 days and Neurobasal/B27 medium (Neurobasal Medium, Thermo Fisher Scientific; supplemented with 1× B27, Thermo Fisher Scientific; 1× Glutamax, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: Gravid adult worms were dissected in 1x M9 with a 22G x 1-1/2” needles (Fisher Scientific catalog number 14-840-91) to release embryos ...
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bioRxiv - Biophysics 2023Quote: ... cells were resuspended in the appropriate amount of maintaining medium (RPMI Gibco® supplemented with 10% FBS, 2 mM GlutaMAX Sigma®, 1% Penicillin/Streptomycin Sigma®, 1 mM Sodium Pyruvate Gibco) and plated to allow maintenance and amplification ...
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bioRxiv - Genomics 2023Quote: ... ESC were adapted for > 2 weeks to N2B27 serum-free medium consisting of 1:1 mix of neurobasal medium (Gibco, Cat. No 1103049) and DMEM/F12 (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Human endothelial cells in muscles were stained with biotinylated Ulex Europaeus Agglutinin I Lectin (UEA I, B-1065-2, Vectorlab, 1:100 dilution) and FITC-streptavidin (11-4317-87, Invitrogen, 1:250 dilution). Nuclei was stained with DAPI ...
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bioRxiv - Biochemistry 2024Quote: ... and 15 U of RNA were diluted 1:1 with 2 mM CaCl2 and digested with 100 U Micrococcal Nuclease (Thermo Scientific™ EN0181) at 22 °C and 1’400 rpm for 15 min ...
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bioRxiv - Immunology 2023Quote: ... fresh media was supplemented with 30 U/mL of IL-2 and a 1:1 ratio of anti-CD3 and -CD28 Dynabeads (Thermo Fisher Cat# 11131D) and similarly treated with aphidicolin ...
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bioRxiv - Neuroscience 2023Quote: ... and maintained in N2B27 media (1:1 of DMEM F12 Glutamax [Thermo Fisher Scientific, 10565018] and Neurobasal media [Thermo Fisher Scientific, 12348017], 1X N-2 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with KIF-encoding plasmid and empty vector pcDNA3.1 (+) (1 µg total DNA) using 2 µl Lipofectamine 3000 Reagent and 2 µl P3000 (Thermo Fisher Scientific). hTERT-RPE1 cells stably expressing SMO-tRFP were transfected a day after seeding and IMCD3 cells expressing mCherry-Arl13b were reverse transfected at the time of seeding ...
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bioRxiv - Neuroscience 2020Quote: ... A square glass window (2 mm x 2 mm) of No 1 cover glass (0.13 to 0.17 mm thick, Thermo Fisher Scientific, USA) was placed over the craniotomy and the edges were sealed with cyanoacrylate glue (3M ...
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bioRxiv - Genomics 2023Quote: ... were added into a fresh 2 mL microcentrifuge tube and washed with 1 mL of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific) for a total of three washes (1 min incubation/each) ...
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bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
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bioRxiv - Genomics 2023Quote: ... total RNA from young livers were spiked with 2 µl of a 1:100 dilution of ERCC Ex-fold Mix 1 while that from old livers were spiked with 2 µl of 1:100 dilution of ERCC Ex-fold Mix 2 (Thermo Fisher). Exfold ERCC spike-ins are provided in two mixes ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...