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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... An α-Mouse IgG2a-AF488 secondary antibody (Invitrogen, CAT#: A-21131; 1-4 hours, 25°C), cells were washed 3 times ...
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bioRxiv - Immunology 2024Quote: ... Th1 consisted of 1 µg/mL anti-murine IL-4 (Cat# 16-7041 Invitrogen Carlsbad, CA), 5 ng/mL murine IL-2 (Cat# 212-12 Peprotech Cranbury ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection of MCF-7 and MCF-7/ADR cells was performed with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Hematopoietic precursor cell-7 (HPC-7) cells were grown in IMDM medium (12440-053, Invitrogen) with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... and single cell suspensions stained with 7-aminoactinomycin D (7-AAD; ThermoFisher Scientific, Waltham, Massachusetts) were analyzed on an LSR II flow cytometer (Becton Dickinson).
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bioRxiv - Immunology 2020Quote: ... Cell death was assessed by 7-aminoactinomycin D (7-AAD, Affymetrix eBioscience, San Diego, CA) staining and flow-cytometry (FACSCantoII™ ...
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bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T/17 (ATCC® CRL-11268) and LLCMK-2 (monkey kidney epithelial cells, ATTC CCL-7) were maintained in Tissue Culture Medium (DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Sigma) ...
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bioRxiv - Biophysics 2019Quote: ... N-((2-(iodoacetoxy)ethyl)-N-Methyl)- amino-7-Nitrobenz-2-Oxa-1,3-Diazole (IANBD ester) and Oregon Green 488 maleimide were purchased from LIFE TECHNOLOGIES LTD (Paisley ...
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bioRxiv - Immunology 2021Quote: ... and then transferred to nitrocellulose membrane for 7 min at 20 V using the iBlot 2 Dry Blotting System (Invitrogen). Where protein phosphorylation status was investigated ...
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bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured for 7 days in Tu 2% media (1205Lu) supplemented with lipid depleted FBS (Omega Scientific, Fisher Scientific). Dox inducible LBR KD was initiated 3 days prior to the experiment ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... 7% of heat-inactivated fetal bovine serum (FBS) and 2% of G-418 disulfate 50mg/mL (all from ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: The staining of oxidants was performed with the commercially available 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (#C6827, Thermo Fisher Scientific, USA). Cryo-sections (10 µm ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: A549 cells encapsulated in hydrogels were stained at the beginning (Day 7) and the end of culture (Day 28) with 2 µM Calcein-AM (Invitrogen) and 30 µg/mL propidium iodide (PI ...
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bioRxiv - Plant Biology 2024Quote: Quantification of ROS in guard cells of epidermal peels was measured using 20 µM 2’,7’-dichlorodifluorescein diacetate (H2DCFDA; Invitrogen) according to Pornsiriwong et al ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...
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bioRxiv - Molecular Biology 2020Quote: ... 3) and four tyr (clutch no. 7) mutants using the Invitrogen TOPO TA kit according to the manufactures protocol (Invitrogen catalogue no. K4575J10), and Sanger sequenced the extracted plasmids from 6-10 colonies per embryo (Fig ...
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bioRxiv - Bioengineering 2023Quote: The number of epithelial cells in the Cervix Chip was obtained by digesting the Cervix Chip apical channel at day 7 of differentiation or day 3 on co-culture with bacteria with Collagenase IV (Gibco, Cat. No. 17104019) in TrypLE (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... cells previously seeded overnight were incubated for 4 h with 10 μM Alexa Fluor™ 594-dextran (Invitrogen, D22913) and analysed in live by laser confocal microscopy.
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bioRxiv - Bioengineering 2021Quote: ... and was fixed in 10% neutral-buffered formalin (NBF) at 4 °C for 72 h and stored in PBS (pH 7.4; Gibco) at 4 °C for subsequent NMR spectroscopy and imaging experiments.
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bioRxiv - Biophysics 2020Quote: ... 20 mM MES pH 6.0) at 4 °C for 12 h using 3.5 kDa MWCO Slide-A-Lyzer MINI Dialysis Devices (Thermo Scientific) to remove imidazole and CHAPS ...
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bioRxiv - Cell Biology 2019Quote: Cells were seeded 18 h before transfection on sterile 4 chamber dishes at 60% confluency.Transfection was carried out using Lipofectamine2000 (Invitrogen) and DNA plasmid at the ratio of 3:1 in HeLa and 2:1 for 293T cells and U2OS cells with total DNA of 1500 ng for imaging and 2000 ng for western blot ...
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bioRxiv - Cell Biology 2019Quote: ... for 12 h at 4°C and then Alexa Fluor 647-conjugated goat anti-rabbit (Thermo-Fisher Scientific Inc.) for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... solution and spun for 1.25 h at 41,500 x g at 4°C in a Sorvall S55-A2 fixed angle rotor (ThermoFisher Scientific). Cell and virus fractions were lysed in lysis buffer (30 mM NaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor specimens were minced and digested for up to 4 h at 37°C in AdMEM/F12 medium (Gibco) containing 2 mM GlutaMAX (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Transcription reactions were incubated 4 h at 37 °C and transcripts were purified with TRIzol Reagent (Thermo Fisher Scientific) and Direct-zol RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates were incubated with RNA-aptamer-coupled beads for 3.5 h at 4°C under rotation in the presence of 60U RNasin (Ambion). For each pull-down ...
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bioRxiv - Neuroscience 2020Quote: ... mice were first anesthetized with a ketamine (100 mg/kg) and xylazine (7 mg/kg) mixture then perfused with 4% paraformaldehyde (Thermo Fisher Scientific), and the brains were extracted and cryoprotected in 30% sucrose in 1X PBS until they sank ...
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bioRxiv - Microbiology 2021Quote: ... 7) and plunge-frozen at a condition of 95% humidity and 4°C by using a Vitrobot Mark IV (Thermo Fisher Scientific). The cryo-EM data were acquired with a Titan Krios at 300 kV (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... but the blastocysts were incubated in 10 μM 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC; Cell Tracker Blue, Life Technologies, Carlsbad, USA). Fluorescence intensity for GSH was measured under a Nikon scanning confocal microscope with a filter at 371 nm excitation and 464 nm emission ...
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bioRxiv - Neuroscience 2021Quote: ... with the substrate tertiary-butyloxycarbonyl-leucine-methionine-7-amino-4-methylcoumarin (abbreviated to t-BOC-Leu-Met-CMAC, or in short: CMAC) (Invitrogen, Carlsbad, CA), at a concentration of 50 μM in the culture medium ...
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bioRxiv - Physiology 2021Quote: Total RNA from around 20 whole flies of both sexes at 4-7 days old was extracted using a PureLink RNA Mini Kit (Cat# 12183020, ThermoFisher Scientific, USA). Reverse transcription (RT ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Biochemistry 2024Quote: ... The grids were blotted using filter paper for 7 seconds at 4°C under 100% humidity using Vitro Mark IV (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted from 4 or 5 pools of 10 embryos at 7 dpf and 14 dpf using Trizol (Life Technologies Europe) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Immunology 2022Quote: ... tissues were minced with a razor blade and subjected to a 30-minute enzymatic digestion at 37 °C in 3 mL of 1:1 DMEM/F12 media (Gibco, Grand Island, NY; 200 mM L-glutamine) with 1X-collagenase/hyaluronidase (10X stock ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: Alexa Fluor 488-conjugated goat anti-chicken IgY (H+L) (Invitrogen, A-11039, 1:1000) and Alexa Fluor 555-conjugated donkey anti-rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Serum was used in a 1:100 dilution and Alexa Fluor 488-goat anti-rabbit IgG (H+L) (Invitrogen; A-11034) 1:5,000 ...