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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, Waltham, MA, USA) was used to label all nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... 0.25 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) was added to immunolabeled cells for 10 min ...
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bioRxiv - Immunology 2023Quote: ... and counterstained with DAPI (4’, 6-diamidino-2-phenylindole, Thermo Fisher Scientific). Sections were examined with a Nikon A1 confocal microscope and LY molecule tissue infiltration (depicted in green channel images included in Fig 4C ...
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bioRxiv - Developmental Biology 2023Quote: ... with 4′6-diamidino-2-phenylindole (DAPI, 00-4959-52, Thermo Fisher), was used to visualize nuclei and as mounting medium ...
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bioRxiv - Cancer Biology 2023Quote: ... 4′,6-Diamidino-2-phenylindole (DAPI; D1306; Thermo Fisher Scientific, Waltham, MA) was used to stain cell nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were counterstained with DAPI (4’,6-diamidino-2-Phenylindole, Dihydrochloride - Invitrogen) for staining nuclei ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific: D1306) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... incubated with 4′,6-diamidino-2-phenylindole (DAPI) as directed (Invitrogen, D1306), and washed with PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained with 4′,6′-diamidino-2-phenylindole (DAPI) (Thermo Fisher) nuclear stain diluted in PBS for 5 min with rocking ...
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bioRxiv - Bioengineering 2023Quote: ... 2 μL of a NeutrAvidin solution consisting of 4 μg NeutrAvidin (ThermoFisher) dissolved in 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM MS(PEG)4 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) at 30 °C for 45 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 2-hydroxyazobenzen-4’-caryboxylic acid (HABA)-avidin complex was used (Thermo Fisher). Quantification was measured at 500 nm ...
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bioRxiv - Bioengineering 2023Quote: ... Slowfade gold antifade mountant containing 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) was used to rinse and seal the coverslips ...
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bioRxiv - Microbiology 2023Quote: ... the fluorescent dsDNA-labelling dye 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher) was added to the culture at a concentration of 5 µg.mL−1 for 10 minutes prior imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were counterstained for 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, R37606) for 5-10 mins before mounting ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 µL of Herpes solution and 2.5 mL of 100 mM CaCl2), staining with Annexin V PE (BD Pharmagen™, BD Biosciences, CA, US and 1 mM 7-Aminoactiomycin D (Thermo Fisher Scientific) and analysis by Flow Cytometry using a BD LSRFortessa X20.
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bioRxiv - Cell Biology 2022Quote: ... and using the Applied Biosystems ViiA 7 Real-Time PCR System and the appropriate primers for Taqman assays (Life Technologies; Supplementary Table 1). The relative gene expression was calculated using the 2-ΔΔCt quantification method after normalization to GAPDH values and expressed as fold of levels found in undifferentiated hPSCs cells for cultured cell or in WT mice group for liver analysis.
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides, Thermo Scientific): [Biotin-7-dATP ...
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bioRxiv - Immunology 2024Quote: ... then collected and plated 10:1 with allogeneic DC-10s in 24-well plates at a concentration of 7 × 105 to 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco, Thermo Fisher Scientific). For CRISPRa experiments ...
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bioRxiv - Biochemistry 2023Quote: ... at ~10 mg ml-1 concentration and blotted for 5-7 s followed by plunge-freezing into liquid ethane using a Vitrobot MarkIV (Thermo Fisher Scientific, USA) operating at 100 % humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... referred to as ENAS medium (supplementary table 1) PDTs were routinely passaged at intervals of 7 days using TrypLE (Gibco, Cat. No. 12605010) and pipetting to dissociate the cells.
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After the secondary antibody another four 15min washes in PBST (here 0.5% tween) with 1% BSA were performed followed by a short 5min incubation in TO-PRO™-3 Iodide (642/661)(Invitrogen, cat# T3605, 1:1000 dilution). The animals were mounted in moviol with DAPCO on glass slides and stored at 4°C till imaging.
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bioRxiv - Genomics 2020Quote: ... Abl.3 and Abl.4 (13) were cultured in Roswell Park Memorial Institute medium (Gibco), containing 15% FBS (Sigma) ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: HAEC cells (passage 3-4) were lysed in ice-cold RIPA buffer (ThermoFisher, cat# 89900) containing protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell number was determined every 3-4 days using the Countess automated cell counter (Invitrogen). 20,000 cells were then re-plated with fresh media and compound ...
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bioRxiv - Neuroscience 2019Quote: ... Lines were passaged every 3-4 days using enzymatic detachment with Stempro Accutase (ThermoFisher; A1110501) for 5 minutes and re-plated in mTeSR1 medium with 10µM Rock Inhibitor (Y2732 ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from approximately 3-4 x 107 cells using TRIzol reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... for 3-4 hr in the presence of brefeldin A (BFA; 10ug/mL; Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... 3-4 μm paraffin sections were prepared with a HM 355S microtome (Fisher Scientific, 10862110), deparaffinized and rehydrated up to 96% ethanol (v/v) ...
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bioRxiv - Immunology 2023Quote: ... Cell passaging was performed every 3 to 4 days using 0.05% Trypsin-EDTA solution (Gibco). Expi293F cells were maintained in Expi293 Expression Medium (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at day in vitro (DIV)3-4 using Lipofectamine 2000 (Invitrogen). Shortly ...
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... followed by a second round of transfection 3-4 hours later using RNAiMax (Life Technologies) according to the manufacturer’s instructions with final siRNA concentration of 50 nM and 20 nM for Sac2 and OSBP ...
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bioRxiv - Cancer Biology 2023Quote: ... for 3-4 days before they were collected directly in TRIzol reagent (Invitrogen cat#15596026). Before collection ...
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bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).
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bioRxiv - Genetics 2022Quote: ... The styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64, 514/670 nm absorption/emission Invitrogen™, Waltham, Massachusetts) was used at a final concentration of 16.5 μM in ddH2O from a 16.5 mM stock solution in DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 x 2 mins and blocked with SeaBlock blocking buffer (Thermo Fisher) for 15 mins at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... particle size = 3 μm, C18, L = 2 cm; analytical column: particle size = 2 μm, C18, L = 50 cm; PepMap, Dionex/Thermofisher). Peptides eluting from the column were ionized using an Orbitrap Elite (Thermofisher).
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bioRxiv - Biochemistry 2020Quote: ... Following 2 weeks of selection in 250 μg ml−1 Hygromycin B (Thermo Fisher) and 4 μg ml−1 Blasticidin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate equivalent to 1-2 × 105 cells was resolved by SDS-PAGE (Life Technologies) and transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by staining using 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI; Thermo Scientific) for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... and F(ab’)2 goat anti-mouse Alexa Fluor 633 (Molecular Probes, 1:1000).
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Molecular Biology 2021Quote: ... Preamplified products were treated with 4µl of 1:2 diluted ExoSAP-it (Affymetrix Inc.) and incubated for 30 min at 30°C ...
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bioRxiv - Neuroscience 2021Quote: ... THP-1 macrophages were labeled with 2 μM CellTracker Green CMFDA dye (Invitrogen, C2925) in HBSS containing Ca++ and Mg++ (Gibco ...
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bioRxiv - Immunology 2019Quote: ... These cells (2 × 106 cells/ml) were incubated with 1 ng/ml TNFα (Gibco) and allowed to adhere to channels coated with fibronectin (Sigma-Aldrich)35 ...