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Citations for Thermo Fisher :
4651 - 4700 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies (1:1000) used were: goat anti-mouse IgG(H+L) Alexa Fluor 594 (Thermo Fisher Scientific, A-11005).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected 48 h later for lysis and reverse transcription was performed using the Cells-to-Ct kit (Applied Biosystems) directly in 96-well plates ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were transfected with indicated plasmids alone or in combination for 8 h by using Lipofectamine 3000 (Thermo Fisher, USA), and then continue to cultivate for 24 h in the fresh medium before being subjected to indicated experiments.
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of the plasmid was digested with KpnI and BamHI in a 20 μL reaction for 1 h using FastDigest enzymes (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA from untreated and 1.5 h rapamycin treated cells prepared as described above was synthesized into cDNA using SuperScript™ III Reverse Transcriptase (18080093, ThermoFisher). cDNA was diluted 1:10 before the quantitative RT-PCR was performed ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was eluted with nuclease free water at 12000 x g for 2 min after which 1 µg of RNA was transcribed into cDNA using the RevertAid H Minus First Strand Kit (ThermoFisher Scientific) and random primers ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... 500 ng of total RNA was reverse transcribed using an oligo(dT) primer and the enzyme “Maxima H Minus” (both from Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... was added as the secondary antibody at a 1:2000 dilution for 1 h at 37C, followed by adding TMB (3, 3, 5, 5’-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) for about 15 min ...
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bioRxiv - Genomics 2020Quote: ... for 1 h at 4 °C and immunoprecipitations were performed with 30 μL of Pierce Anti-HA Agarose (Thermo Fisher Scientific) per sample rotating overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Sixty nanograms of total RNA were reverse transcribed by using the RevertAid H Minus First Strands cDNA Synthesis Kit with oligo(dT) primers (Thermo Scientific). The obtained cDNA was amplified using specific primers (Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were incubated for 1 hour at room temperature in appropriate secondary antibodies: Goat anti-mouse IgG (H+L) HRP (ThermoFisher Scientific) or Goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2021Quote: ... antibody at 5 μg mL−1 in BSA 1% m v−1 in PBS for 1 h at room temperature, then with anti-rabbit Alexa 568 (LifeTech, A11036) and Hoechst (Invitrogen, H3570) 1 μg mL−1 in BSA 1% m v-1 in PBS for 1 h ...
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bioRxiv - Genomics 2021Quote: Synthesis of cDNA was carried out with 500 ng of total RNA added to each 20 μl reaction of the RevertAid H Minus Reverse Transcription Kit (Thermo Scientific). The cDNA samples were stored at −20°C for downstream analysis ...
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bioRxiv - Genetics 2021Quote: cDNA was generated from total RNA using Maxima H Minus first strand cDNA synthesis with dsDNase Kit (Thermo Scientific™ K1681) with 1 μg RNA from each sample ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mounted sections were immediately stained with hematoxylin and eosin using the Thermo Scientific Shandon Rapid-Chrome H & E Frozen Section Staining Kit (Thermo Fisher). Stained sections were imaged at 10x with a Leica DM IL LED microscope ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS then suspended in goat anti-mouse IgG (H&L) cross-adsorbed secondary antibody Alexa Fluor 488 (2 mg mL−1; Thermofisher Scientific) in PBS 0.1 % BSA for 45 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the CST staining protocol was followed with mouse anti-human phosphorylated p38 MAPK (Clone: Thr180/Thr182) used with goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (Thermo Fisher) as a secondary ...
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bioRxiv - Biochemistry 2020Quote: ... were then combined into expression constructs containing MAT1-Cyclin H-CDK7 using PmeI (MssI) and SwaI (SmiI) restriction digests (FastDigest enzymes, ThermoFisher Scientific) and subsequent in-fusion reactions ...
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bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and donkey anti-mouse IgG (H+L, Highly Cross-Adsorbed, Alexa Fluor 488; 1:500, catalog: A-21202, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated anti-mouse IgG (H+L) made in horse and biotinylated anti-mouse IgG2a made in goat (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated anti-mouse IgG (H+L) made in horse and biotinylated anti-mouse IgG2a made in goat (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Slides were washed 3 times in 1× PBS at RT and incubated in the combination with secondary antibodies Alexa 488-conjugated goat anti-rabbit IgG (H+L) (Molecular Probes) and Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... or cDNA plasmids were transiently transfected for 48 h using Lipofectamine RNAiMAX or Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s protocols.
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... was added together with labelled α-bungarotoxin (to visualise post-synaptic acetylcholine receptors) in blocking solution for 1.5 h at room temperature (RT) (α-btx, Life Technologies). Then ...
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bioRxiv - Biochemistry 2021Quote: ... GP-1 was detected by incubating the slide with 2 μg/mL of Alexa Fluor 633 goat-anti-mouse (H+L) antibody (Invitrogen A21050) for 3 h ...
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bioRxiv - Bioengineering 2021Quote: ... Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher). Cells were incubated with 100 nM TO-1 in HBSS starting 30 minutes prior to imaging and for the duration of the imaging period ...
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bioRxiv - Biochemistry 2020Quote: ... Eluates were heated to 65°C for at least 6 h to reverse the formaldehyde cross-linking and then treated with proteinase K (Life Technologies). DNA fragments were isolated using a DNA purification kit (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... followed by washes and detection with Alexa488-conjugated goat anti-mouse IgG(H+L) Cross-Adsorbed Secondary Antibody (Invitrogen A-11001), essentially as described previously (Siggers et al ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in serum before incubation with mouse monoclonal β-endorphin primary antibody (Abeam ab54205,1:200) for 24 h at 4°C and Alexa-fluor 488 chicken anti-mouse secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were incubated 2 h in DMEM-10% horse serum and then changed to MACS neuro medium (Miltenyl Biotec) with B27 supplement (Invitrogen, France). Rat hippocampi were dissected from E18 embryos ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then rinsed three times with 0.05% Tween-20 in PBS (vol/vol) and incubated with the appropriate secondary antibodies for 1 h at room temperature and counterstained with DAPI (Life Technologies).
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bioRxiv - Neuroscience 2020Quote: ... and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat# NP0001-02). At the end of electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C for 24 h and then with an Alexa Fluor® 488-conjugated goat anti-rabbit secondary antibody (1:500, A11034, Invitrogen) at room temperature for 2h ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum slices were further stained for 1-2 h at RT with NeuroTrace 435/455 (1:100 diluted in PBS, Invitrogen, #N21479), a fluorescent Nissl staining ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 h at RT in the secondary antibody (1:1000, goat anti mouse Alexa Fluor 647, Life Technologies) carrier solution (1x PBS ...
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bioRxiv - Biophysics 2020Quote: ... The expression culture was finally centrifuged for 10 min at 4°C and 5316 × g (Thermo Scientific H-12000 BioProcessing Rotor). The cell pellet was separated from the supernatant and resuspended with 40 ml HEPES-buffer per 2 l culture on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific) following addition of 4 mg/mL polybrene (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with secondary antibodies (anti-Mouse IgG 488, 1:1000 Invitrogen, #A11029; anti-rabbit IgG 488, 1:1000, Invitrogen, #A11034), followed by DAPI staining (5 μg/ml).
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription primed by random hexamer primer was conducted using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). SensiFAST™ SYBR® Hi-ROX Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... and incubated for 1 h at RT with a mixture of Alexa Fluor 594 conjugated anti-rabbit IgG (1:500; A11012; ThermoFisher, USA) and Alexa Fluor 488-conjugated anti-mouse IgG (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Infections of epithelial cells (A549 and NuLi-1) were performed two days after seeding with starvation for 16 h before infection using RPMI 1640 Medium (Gibco 21875) supplemented only with 10 mM HEPES.