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Citations for Thermo Fisher :
4801 - 4850 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and BMDCs were incubated at a ratio of 10 microspheres per cell for 6 h with 1.0-μm FluoSpheres® carboxylate-modified microspheres (ThermoFisher, F8851) labelled with a red fluorescent dye (580 nm excitation and 605 nm emission) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Cell Biology 2020Quote: Total RNAs were extracted using Trizol reagent and 1 μg of RNA was reverse-transcribed with the RevertAid H Minus First Strand cDNA Synthesis kit (Life Technologies). qPCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Genomics 2021Quote: ... washed twice with 80% ethanol and resuspended in 8 μl of nuclease-free water before in vitro transcription at 37 °C for 14 h using the MEGAScript T7 kit (Invitrogen, AM1334). Library preparation was done as described in the CEL-Seq2 protocol with minor adjustments (Hashimshony et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 2×106 cells were cultivated for 24 h in presence of either Human T-Activator CD3/CD28 dynabeads (Thermo Fisher Scientific), according to manufacture’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated in absence of light for 1 h with fluorescent goat anti-mouse (1:500, Alexa Fluor 488, Life Technologies) diluted in TBS with 3% BSA ...
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bioRxiv - Physiology 2021Quote: ... The muscle sections were then washed three times in PBS before being incubated for 1 h in the dark at room temperature with Alexa Fluor 488 IgG secondary antibody (A-11008, 1:500; ThermoFisher Scientific) diluted in blocking solution ...
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bioRxiv - Systems Biology 2021Quote: ... Monolayers were then incubated for 2 h with U937 monocytic cells pre-labelled according to manufacturer’s instructions with CMFDA cell tracker dye (ThermoFisher Scientific, UK). Co-cultures were washed vigorously with ice-cold PBS three times and fixed by incubation for 10 min in 1 % formaldehyde in 0.1 M PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were washed twice with PBS and labeled with a secondary antibody (Alexa Fluor 488 donkey anti-Goat IgG (H+L) (A11055, 1:200, Life Technologies) in PBS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in TBST buffer with 0.5% milk and goat α-rabbit IgG (H+L) HRP conjugated secondary antibody (1:10,000, ThermoFisher Scientific 31460) in TBST were used to detect PstP-strep ...
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bioRxiv - Microbiology 2020Quote: ... The antigens were then reacted with an Alexa Fluor 488-conjugated goat anti-mouse IgG H+L antibody (Thermo Fisher Scientific). The antibody titer was defined as the reciprocal of the highest dilution level at which a specific fluorescent signal was detected.
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bioRxiv - Bioengineering 2021Quote: ... Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Thermo Fisher Scientific, A32731, 1:1000), PNA (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... cells were incubated for 1 h at 37°C with secondary antibodies: Goat anti-Mouse Alexa Fluor 488 (ThermoFisher, A-11001) and Donkey anti-Rabbit Alexa Fluor 555 (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: 15 pmol of RNA/DNA were radioactively labeled for 1 h at 37 °C using 5 U T4 Polynucleotide Kinase (Thermo Fisher) and 10 µCi γ-32P ATP ...
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bioRxiv - Biochemistry 2021Quote: ... and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat# NP0001-02). At the end of electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.5) at 4°C for 24 h using the Slide-A-Lyzer mini dialysis devices (20K MWCO, Thermo Fisher Scientific). After dialysis ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Immunology 2021Quote: ... Cat#PA1-8463) or goat-anti-monkey IgG (H+L, Cat#A18811) secondary antibody conjugated to horseradish peroxidase (HRP, Thermo Fisher) in blocking buffer at 1:10,000 or 1:4,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Physiology 2021Quote: ... followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121, Thermo Fisher, 1:50) and/or rabbit polyclonal TOM20 antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... Australia) primary antibody and a donkey anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor 568 (A10037; ThermoFisher, France), essentially as described (28) ...
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bioRxiv - Genomics 2020Quote: ... One to two micrograms of RiboMinus RNA of each sample were used for cDNA synthesis with the Maxima H Minus double-stranded cDNA synthesis Kit (Thermo Scientific) primed with random hexamers for samples Acer+ (2014 ...
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bioRxiv - Cell Biology 2021Quote: ... (Cai et al., 2009), or Kif15 (5’-GGACAUAAAUUGCAAAUAC-3’, 24 h) (Vanneste et al., 2009) using Lipofectamine RNAiMAX (13778075, Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... (diluted 1:400 in 2% BSA) and a second step of 1 h of incubation with an Alexa Fluor 488 conjugate secondary antibody (Molecular Probes) (diluted 1:1000 in 2% BSA) ...
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bioRxiv - Microbiology 2021Quote: ... The plates were grown at 37°C for 24 h on a shaking platform at 200 RPM either aerobically or anaerobically (using Thermo Fisher AnaeroPack Anaerobic Gas Generators in a sealed plastic bag) ...
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bioRxiv - Genomics 2020Quote: ... The cells were recovered for 48 h with no antibiotic followed by selection in puromycin (1 μg/mL) (Thermo Fisher, A1113803). Single colonies were manually picked and expanded in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... The recovered bacteria were serially diluted in MH broth and plated onto MH agar plate followed by incubation at 42 °C for 24 h under the microaerophilic conditions in a tri-gas incubator (Thermo Scientific). Bacterial colonies that appeared on the plate were counted to enumerate colony-forming units (CFU) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were reduced by incubation with 200 mM TCEP at 55 °C for 1 h and alkylated by incubation with 375 mM iodoacetamide (IAA) (Thermo Scientific) for 30 minutes while protected from light at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... After 2 washes cells were incubated for 1 hour with 0.5 μg/ml of Alexa Fluor 555 goat anti-mouse IgG (H+L) (ThermoFisher Scientific, #A21424). After 2 washes cells were resuspended in PBS and analyzed by flow cytometry using a FACS BD LSR-Fortessa.
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were washed again as described and then incubated with secondary donkey anti-mouse IgG (H+L) Alexa Fluor 594 (Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Immunology 2020Quote: ... T cells were first given αCD3/αCD28 stimulation for 24 h in the form of CD3/CD28-coated Dynabeads (1 bead:100 cells; Gibco, #11161D). The beads were then removed with a magnet ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate first-strand cDNA using Thermo Scientific Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase (Thermofisher), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647 (Jackson Immuno Research; Ref# 705-605-003) and donkey anti-rabbit IgG (H+L) Alexa Fluor 647 (Invitrogen; A32733). Flowcytometry was performed on a Becton Dickinson FACS Calibur and analyzed by Flow Jo.
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bioRxiv - Immunology 2020Quote: ... Secondary staining’s were performed for 1 h at room temperature in the dark with biotinylated goat anti-rabbit IgG AlexaFluor 405 (1:400; A-31556, Life Technologies) and goat anti-mouse IgG1 AlexaFluor 647 (1:800 ...
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bioRxiv - Immunology 2020Quote: ... 1μg of total RNA was reverse-transcribed to cDNA using the Maxima H Minus First Strand cDNA Synthesis Kit (ThermoFisher Scientific). qPCR was carried out using SYBR Select Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked for 3 h at room temperature in PBS with 3% powdered milk and 0.05% Tween 20 (Thermo Fisher Scientific). Protein detection was performed using the anti-EBOV GP (ZGP 12/1.1 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times 1x PBS then incubated for one-hour room temperature with goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 (Invitrogen; A32731TR). After washing three times for 5 minutes with 1x PBS cells were fixed to glass microscopy slides (Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and the supernatant incubated during 4 h at 4°C with 50 µl of V5 magnetic beads (20 mg/mL of Dynabeads® M-280 Tosylactivated, Invitrogen, coupled with Anti V5-Tag Antibody ...
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bioRxiv - Biophysics 2021Quote: ... Cleavage reactions were incubated at 37 °C for 1 h and the reaction was stopped by the addition of 0.36 U Proteinase K (Thermo Fisher Scientific), followed by incubation at 55 °C for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... After washing cells were incubated with secondary antibody for 1 h at RT (Alexa Fluor 488 goat anti-rabbit, Invitrogen, R37116). Finally ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 50 nM final siRNA for 72 h using Lipofectamine RNAiMax according to the manufacturer’s guidelines (Thermo Fisher Scientific). siRNA Oligonucleotides were synthesised by Merck (sequences are given in the Supplementary Table S1).
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bioRxiv - Cell Biology 2021Quote: ... cryo-ET data of nucleocapsids in situ at control and ~1 h of 0.5 mM As(III) stress were also collected with a Volta phase plate58 (VPP, Thermo Fisher Scientific) for the purpose of better visualization ...
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bioRxiv - Biochemistry 2021Quote: ... Then the F-actin was incubated for 2 h at room temperature with a five-fold molar excess of Alexa-488 NHS ester dye (Life Technologies). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed for 5 minutes 3 times with PBS followed by incubation with goat anti-Rat IgG (H+L) Alexa Fluor 594 secondary antibody (A11012, Thermo Fisher) at 1:500 dilution for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg plasmid DNA per well was transfected into cells for 16 h at a confluency of 60% in 6-well plates using Lipofectamine 3000 (Invitrogen, L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... Vesicles were subjected to high-speed centrifugation for 4 h at 55,000 g (25°C) and subsequently at 8 h at 75,000 g (4°C) with an ultracentrifuge (Sorvall RC M150GX, Thermo Fisher Scientific) using a fixed angle rotor (S150AT-0128 ...