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Citations for Thermo Fisher :
4851 - 4900 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used in these experiments are goat anti-rabbit 488 and goat anti mouse H+L 568 (Life Technologies) at 1:1000 and goat anti guinea pig 568 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed using Maxima H minus first strand cDNA synthesis kit with dsDNase according to the manufacturer (Thermofisher Scientific). Genomic DNA was extracted using QIAGEN DNeasy Blood and Tissue kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... were detected with mouse anti-Opa 4B12 and mouse anti-porin H5.2 respectively followed by goat anti-Mouse IgG (H+L) Dylight 800 cross adsorbed secondary antibody (Invitrogen SA5-10176). All antibodies were diluted in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... first-strand cDNAs were synthesized from the polyA(+) RNA samples using the Maxima H Minus Reverse Transcriptase enzyme (Thermo Fisher Scientific) and the SSP and VN primers (supplied in the ONT kit) ...
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bioRxiv - Microbiology 2023Quote: ... directed against the cytoplasmic domain of E-cad and revealed using an anti-mouse IgG (H+L) secondary antibody (Alexa Fluor 555) (Life Technologies). The 4’,6’-diamino-2-fenil-indol (DAPI ...
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bioRxiv - Immunology 2023Quote: ... in PBS for 1 h at 25°C followed by permeabilization in 5% BSA in the presence of 0.05% Tween-20 (Thermo Fisher Scientific) for 15 minutes at 25°C ...
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bioRxiv - Developmental Biology 2023Quote: ... and 48 h after the onset of the course mESCs were collected and stained with trypan blue for counting (CountessTM, Invitrogen AMQAX1000). Cell counts were performed for three biological replicates per condition during the time course ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with goat anti-rabbit IgG (H+L) HRP conjugated secondary antibody (1:5000) (Thermofisher Scientific, 31460) for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... The instrument was equipped with an electrospray ionization source (H-ESI II Probe) coupled to an Ultimate 3000 RS HPLC (Thermo Scientific). A Thermo Scientific Hypersil GOLD aQ chromatography column (100 mm x 2.1 mm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs bound to the beads were used as a template for cDNA synthesis using Maxima H Minus cDNA first strand synthesis Kit (ThermoFisher, 10338179) and random hexamers as primers ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_2893433), tdTomato (SICGEN, 1:500, RRID: AB_2722750) donkey anti-goat IgG (H+L) Alexa Fluor 488 (Invitrogen, 1:1000, A11057), Cy3 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Plant Biology 2022Quote: ... Qualitatively and quantitatively checked RNA was then used to synthesize cDNA with the RevertAid H minus First Strand cDNA Synthesis Kit (ThermoFisher Scientific). This cDNA (1 μl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature for 3 h in TBS containing AlexaFluor-546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, Fisher Scientific), 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Following repeated washing in PBS slices were incubated in donkey anti-goat IgG H&L (Invitrogen Alexa Fluor 488; 1:500) and donkey anti-rabbit IgG H&L (Alexa Fluor 568 ...
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bioRxiv - Physiology 2023Quote: ... cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; Thermo Fisher Scientific, USA). Overnight incubation with primary antibodies (anti-VPS36 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT10 (Thermo Scientific, #90110) or TMTpro 18-plex (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: Age synchronized worms were washed thrice with 500 μl M9 buffer in 1.5 ml microfuge tube and incubated in dark for 1 h in 1 ml M9 containing 5 µl DiD dye (Vybrant™ DiD Cell-Labeling Solution, ThermoFisher) at ∼20-30 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 24 h before the cells were lysed with 300 µl M-PER Mammalian Protein Extraction Reagent (Thermo Scientific). A volume of 10 µl was resolved on a 3-8% Tris Acetate gel ...
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bioRxiv - Cell Biology 2023Quote: ... After 48 h the cells were lysed and a pulldown assay was performed from cell lysates using either anti-HA (88836, Thermo Fisher) or anti-GFP (gtd-100 ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies were used at 1:1000 at RT for 1 h with rotation (Anti-rabbit-HRP, Invitrogen, anti-Armenian Hamster-HRP, Thermo Fisher). Blots were imaged using an Amersham Imager 600 ...
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bioRxiv - Cell Biology 2023Quote: ... Four hours after transfection the cell culture medium was exchanged and cells were grown for 48 h and selected with 2.5 mg ml-1 blasticidin (Cat.No.: R210-01, Invitrogen, Waltham, Massachusetts, USA). A stable monoclonal line was isolated using blasticidin selection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Slides were washed three times in 1× PBS at RT and incubated in a combination of secondary antibodies (Cy3-conjugated goat anti-rabbit IgG (H+L) (Molecular Probes) and Alexa-488-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed five times with the same blocking solution and then incubated with the corresponding secondary antibodies conjugated with Alexa fluorophores at 4°C with gentle agitation for 24 h (1:1000, Molecular Probes). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were then incubated for 1 h at room temperature with secondary HRP-conjugated goat anti-mouse antibodies (Invitrogen 3430). Antibody-labeled proteins were visualized using SuperSignal West Femto Maximum Sensitivity ECL Substrate (Thermo Fischer Scientific 34094 ...
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bioRxiv - Physiology 2023Quote: After 24 h of hyperosmotic stress each well was stained with 5 µg/mL Hoechst 33342 dye (Catalog 62249, Thermo Fisher), 1 µg/mL Propidium Iodide (PI ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with the following fluorophore-conjugated secondary antibodies for 2 h at room temperature: anti-rabbit Alexa Fluor 488 (1:400, goat, Invitrogen, A11008), anti-rabbit Alexa Fluor 568 (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated with the goat anti-rabbit IgG for 1 h at room temperature before signals were detected using ECL (Thermo Scientific) on the Chemidoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated with the appropriate secondary antibodies for 1 h at room temperature before signals were detected using enhanced chemiluminescence (ECL) (Thermo Scientific) on the Chemidoc MP (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were incubated with the secondary antibodies goat α-rabbit IgG (H&L) Alexa Fluor 488 (1:250, Invitrogen A11008) and goat α-mouse IgG (H&L ...
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bioRxiv - Cell Biology 2023Quote: ... the glasses were incubated for 2 h at room temperature with secondary antibodies (1:800 Alexa Fluor 594 and 647 antibodies from Molecular Probes) and mounted in polyvinyl alcohol mounting medium with DAB-CO [52] ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed with TBST and incubated with the required fluorescent-dye conjugated secondary antibody (1:1000) for 1 h at RT (Alexafluor, Invitrogen, Thermofisher). Slides were mounted with DAPI Pro-LongDiamond antifade mountant overnight and analyzed the next day ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 2 h at RT with AlexaFluor (AF) 488-labeled donkey anti-rabbit antibody (1:500, Invitrogen, Poland) and AF647-labeled phalloidin (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 1 h at 4°C with Pierce™ Protein G magnetic beads (Invitrogen) previously attached to V5 tag antibodies (R960-25) ...
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bioRxiv - Immunology 2023Quote: ... 1:100 fold diluted Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor™ 488, ThermoFisher, Cat.#R37116) was then added and incubated at room temperature for 1 h ...
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bioRxiv - Immunology 2023Quote: ... the membranes were incubated for 1 h at room temperature with Poly HRP-conjugated anti−rabbit or anti−mouse IgG (Thermo Fisher). ECL Plus reagents (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed four times with PBS plus 0.05% Triton X-100 (PBS-Triton) and incubated for 1 h at RT with Alexa Fluor 568 donkey anti-rabbit IgG antibody (Invitrogen, A10042) at a 1:500 dilution in PBS-Triton ...
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bioRxiv - Microbiology 2023Quote: ... The biofilms were then washed once and incubated for 1 h at room temperature with an Alexa Fluor 594-labeled goat anti-mouse IgG highly cross-absorbed secondary antibody (Invitrogen, A32742)) [2 µg mL-1] within BSA blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... The percentage of infected GFP- positive cells was determined 48 h post-transduction by flow cytometry (Attune™ NxT Acoustic Focusing Cytometer, Invitrogen), following cell detachment and fixation with 4% paraformaldehyde (PFA).
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bioRxiv - Neuroscience 2023Quote: ... sections were then incubated for 1 h at room temperature with one of the following fluorophore-conjugated secondary antibodies or streptavidin: donkey anti-mouse Cy3 (ThermoFisher Scientific), goat anti-chicken Alexa Fluor 488 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: The applied MS was a TSQ Quantiva triple quadrupole MS equipped with a heated electrospray ionization source (H-ESI-II probe) both from Thermo Fisher. The vaporizer temperature was set to 210 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was then incubated with a secondary antibody: goat anti-rabbit IgG (H+L) antibody (1:5000; Thermo Fisher Scientific) or Goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 times with PBS and incubated with the following secondary antibodies at RT for 2 h: Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; Molecular Probes) and Alexa Fluor 633-conjugated goat anti-rabbit IgG (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with secondary antibodies at room temperature for 2 h at 1:500 dilution: anti-rabbit AF594 (Invitrogen, A21207); anti-rat AF488 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000, Thermo Fisher Scientific). Images were acquired using the Keyence BZ-X800.
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bioRxiv - Plant Biology 2023Quote: ... 21 ng of total RNA from funiculi of st 17 flowers was used for cDNA synthesis using the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher). The cDNA products were diluted to 0.21 ng/μL with nuclease-free water prior to qPCR.