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Citations for Thermo Fisher :
4601 - 4650 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cells were permeabilized by incubation in 1% Triton X-100 (Cat.No. T8787, SigmaAldrich) in D-PBS +/+ (Cat.No. 14040-091, Gibco) for 15 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... coated with poly-D-lysine at a density of 60,000 cells per 24-well plate (930186, Thermo Scientific). The cells were grown in 500 µl of the Neurobasal Medium (21103049 ...
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bioRxiv - Immunology 2023Quote: ... and SAv-Alexa Fluor 488 were mixed together with 5 µM free D-biotin (B20656; Thermo Fisher Scientific) to minimize cross-reactivity between probes ...
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bioRxiv - Bioengineering 2023Quote: ... mice were administered a dorsal subcutaneous 150 µL injection of 30 mg/mL D-luciferin (Thermo Fisher Scientific) reconstituted in PBS and luminescence images was captured 15 minutes thereafter ...
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bioRxiv - Genetics 2023Quote: ... The isolated sequences were directionally cloned into the pENTR/D-TOPO Gateway entry vector (Invitrogen, Waltham, MA, USA) and transferred by site-specific LR recombination into a specific binary vector (http://www.vib.be/en/research/services/Pages/Gateway-Services.aspx) ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells were seeded at a density of 120,000 cells/well in poly-D-Lysine coated #1.5 German borosilicate Lab-Tek II 4-well chambers (Nunc) in DMEM media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: To induce endoderm differentiation, we washed cultured hESCs with D-PBS (no calcium, no magnesium) (Thermo Fisher Scientific) and treated them with TrypLE Express at 37°C for 3 min ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Biochemistry 2023Quote: ... the filtered supernatant was loaded onto 3-4 mL immobilized D-galactose gel (Pierce™, Thermo Scientific™) equilibrated by gravity flow with Gal A buffer (50 mM Na-phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were checked on 1% agarose gel and directionally cloned using pENTR/D-TOPO Cloning Kit (Invitrogen) and then transformed into 5-alpha competent Escherichia coli cells (NEB) ...
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bioRxiv - Immunology 2023Quote: ... After the indicated time cells were lysed in 1% n-Dodecyl-β-D-Maltoside (DDM, Thermo Scientific #89903) in lysis buffer (30 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% L-glutamine and 4.5g/L D-glucose (Gibco, ThermoFisher) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from 1-to 2-d-old mouse neonates in cold Hank’s buffer solution (HBSS) (Gibco) supplemented with 0.08% D-glucose (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from 1 to 2-d-old mouse neonates in cold Hank’s buffer solution (HBSS) (Gibco), supplemented with 0.08% D-glucose (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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The intersection of endocrine signaling and neuroimmune communication regulates neonatal nociceptionbioRxiv - Neuroscience 2024Quote: ... This process was also carried out with non-targeting siRNAs (Thermo Fisher Scientific, catalog #D-001206-14-05), indicated as siCON.
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bioRxiv - Microbiology 2024Quote: ... A549 and HEK293T cells were maintained in DMEM containing D-glucose (4.5 g/l) and L-glutamine (Gibco), supplemented with 10% heat-inactivated and filter-sterilized fetal bovine serum (ATLAS) ...
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bioRxiv - Plant Biology 2024Quote: ... AC-641/AC-642 and AC-645/AC-646 were annealed and ligated into pENTR-D-TOPO (Invitrogen) following manufacturer’s instructions to generate pENTR-AtmiR173aTS-GUSAt ...
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bioRxiv - Plant Biology 2024Quote: ... The MpRLF coding sequence fragment was cloned into the pENTR/D-TOPO cloning vector (Thermo Fisher Scientific, USA) to produce pENTR-MpRLFcds ...
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bioRxiv - Neuroscience 2024Quote: ... Primary OPCs were plated into poly-D-lysine/laminin-coated coverslips and expanded in neurobasal medium (A2477501, Gibco) with 2% B27 (17504044 ...
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bioRxiv - Microbiology 2024Quote: ... the lungs were gently flushed with 2 × 0.5 ml of sterile Dulbecco’s Phosphate Buffered Saline (D-PBS, Gibco) through trachea cannulation ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were seeded at ∼25% confluency in Lab-Tek 8-chamber #1.0 Borosilicate coverglass system or 35 mm glass bottom dish (poly-D-lysine treated, MetTek Corporation) in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... D-ribose and ribitol were analyzed with high pressure ion chromatography (HPIC) Dionex ICS-6000 (Thermo Fisher Scientific) with CarboPac PA20 column (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... These cells were cultured in DMEM (1X) + GlutaMAX 4.5 g/L D-Glucose (Cat. N. 10566016, Gibco®) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed in D-PBS and incubated with secondary antibodies (1:500, Alexa Fluor™, Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Individualized nanofibers were then resuspended at 5 mg/mL in D-PBS (Dulbecco’s Modified Eagle Media; Gibco, 11966025).
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cell lines at 48 h post incubation with FeNPs or mouse tissues using TRIzol™ (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... were stained with hematoxylin and eosin (H&E) according to standard protocols using the Veristain Gemini Automated Slide Stainer (Thermo Scientific) at the University of Toronto Dentistry Pathology Core ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 2 h at room temperature with the following primary antibodies: rabbit anti-EEA1 (1:3000, Thermo Fisher Scientific Cat# PA1-063A ...
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bioRxiv - Cell Biology 2020Quote: ... at a final concentration of 10 or 20 nmol/L for 72 h using Lipofectamine® RNAiMAX reagent (Invitrogen, 13778-150) in an antibiotic-free EGM-2 medium (without GA-1000) ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: Alexa Fluor 488-conjugated goat anti-chicken IgY (H+L) (Invitrogen, A-11039, 1:1000) and Alexa Fluor 555-conjugated donkey anti-rabbit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... was incubated for 1 h at RT and the signal was detected using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibodies (Goat anti-mouse IgG1 Alexa Fluor 488 conjugate; Invitrogen A21121, Goat anti-rabbit H+L Alexa Fluor 555 conjugate; Invitrogen A21428) with Hoechst 33342 (Thermo H3570 ...
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bioRxiv - Immunology 2021Quote: ... Embryos were then washed with PBST and blocked with 5% goat serum before addition of the secondary antibody (Goat anti-rabbit IgG H&L DyLight 650, 1:200, 84546 Invitrogen, USA) and incubated overnight at 4°C with gentle rocking ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Genetics 2020Quote: ... cDNA was synthesised from 5 µg RNA using random hexamer primers and the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated for 1 h with a secondary antibody (goat anti-rabbit IgG, Alexa Flour 488 conjugate, ThermoFisher Scientific/Invitrogen, R37116) and DRAQ5 (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... reverse transcription was performed using the Maxima H Minus First Strand cDNA Synthesis Kit (K1651, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s directions with 2 μg of total RNA ...
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bioRxiv - Microbiology 2021Quote: ... was seeded with influent solids harvested from the influent of the RAEBL full-scale WRRF by weekly centrifuging 10 L of a 24-h composite influent sample at 4,000×g using the IEC-Centra-8 centrifuge (Thermo Scientific, USA). The total suspended solids (TSS ...
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bioRxiv - Neuroscience 2021Quote: ... slices were fixed for 1 h in PBS with 4% PFA and Streptavidin conjugated to Alexa 647 (1:500, ThermoFisher Scientific) was applied during secondary incubation following blocking and permeabilization.
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000 dilution).
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bioRxiv - Developmental Biology 2020Quote: ... full length cDNA was synthesized using Maxima H Minus first strand cDNA synthesis kit (K1651, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol with 2 μg of total HH37 chick ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 500-2000 ng total RNA using RevertAid™ H Minus M-MuLV Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were incubated with horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG and Goat Anti-Mouse IgG (ZSGB-BIO, Beijing, China) for 2 h and signals were observed using Super Signal West Pico (Thermo Scientific). Western blotting band intensity was quantified by densitometry analysis using ImageJ version 2x (NIH Image software ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed with PBS to eliminate dead cells and then incubated for 1 h in 200 μL of HBSS (#14025092, Gibco, ThermoFisher) containing 100 μM of Resazurin (#B21187 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 h at room temperature with one of the species-matching conjugated secondary antibodies (all from ThermoFisher Scientific): goat anti-mouse Alexa-Fluor 555 (cat ...
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bioRxiv - Neuroscience 2021Quote: We mounted larval and adult nervous systems in VECTASHIELD (Cat. # H-1000) or ProLong Gold antifade with DAPI (Invitrogen, Cat. # P36931). If users prefer to clear with xylenes before mounting ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% Triton X-100 in PBS) for 2 h at room temperature and then with primary antibody (rabbit anti-GFP, 1:1000, Invitrogen, G10362) diluted in blocking buffer overnight at 4℃ ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...