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Citations for Thermo Fisher :
4501 - 4550 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... samples were treated with Alexa Fluor 488 donkey anti-rabbit IgG (H+L) secondary antibody (2 µg/mL, A-21206, Invitrogen) for an hour at RT and washed again with PBS 1× ...
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bioRxiv - Cell Biology 2024Quote: ... and Alexa Flour 488-conjugated goat anti-rabbit IgG (H+L) antibody (1:2000) (Thermo Fisher Scientific, Waltham, MA, USA) was used for the secondary antibody.
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bioRxiv - Bioengineering 2024Quote: ... samples were incubated with Alexa Fluor 488 donkey anti-rabbit IgG (H+L) secondary antibody (2 µg/mL, A-21206, Invitrogen) for 1 hour at RT and then washed again with PBS 1× ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Biochemistry 2024Quote: ... in 6-well plates before transfection with 500 ng ptCMV-EGFP-TRIM plasmids for 4 h in OptiMEM (ThermoFisher, 31985062) and 1.5 μl 1 μg/μl PEI (Sigma ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells were grown to 60% confluency in 6-well plates before transfection with 500 ng pcDNA3.1-FLAG-TRIM plasmids for 4 h in OptiMEM (ThermoFisher, 31985062) and 1.5 μl 1 μg/μl PEI (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... and Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Alexa Fluor Plus 555 (Thermo Scientific, A32727, ICC 1:500). Cells were washed twice for 5 minutes with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice in PermWash buffer and goat anti-mouse IgG (H+L) Alexa Fluor 488 secondary antibody (Invitrogen) at 1:300 dilution in Intercept Antibody Diluent (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... The required nicking of DT was performed by 1 h incubation of DT at 37°C with 1:1 (w/w) trypsin-EDTA (0.25% w/v) from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: RNA from MDA-MB-231 WT and RAB25+ cells was extracted 72 h post-siRNA transfection using GeneJET RNA purification columns (K0732; Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated with the appropriate secondary antibodies: 488/568/633 immunoglobulin G (IgG; H+L) and/or Alexa Flour 568/647 phalloidin (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Lung and tumor samples were either processed immediately or held intact for a maximum of 16 h at 4°C in DMEM/F12 media (Gibco) supplemented with 1 mg/mL of penicillin and streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by washes and incubation for 1 h at RT with a secondary antibody conjugated to Alexa 488 or 568 (anti-rabbit Alexa 568, A11011, Invitrogen, 1/2000 and anti-mouse Alexa 488 ...
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bioRxiv - Cancer Biology 2024Quote: ... fluorescently conjugated secondary antibodies were added for one hour at RT by diluting in blocking buffer as follows: Goat anti-Chicken IgY (H+L) Alexa Fluor 488 (1:500, A-11039, Invitrogen), Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (H+L) Cross adsorbed secondary antibody with Alexa Fluor 555 (A-21428) [1:500] all purchased from ThermoFisher Invitrogen.
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bioRxiv - Genetics 2024Quote: Isolated or thawed naïve T cells were kept in basal media for 24 h and then activated by adding Dynabeads Human T-Activator CD3/CD28 (1:1 bead-to-cell ratio, Gibco), 300 U/ml recombinant human IL-2 (Peprotech) ...
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bioRxiv - Cell Biology 2024Quote: ... or 360,000 cells/well (24 well plates) and cultured for 8 h in Dulbecco’s modified Eagle medium (DMEM) (Life Technologies Corporation) containing 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... MCs were cultured in 12-well plates for 72 h in 1 mL phenol red-free RPMI medium (Gibco, USA) to avoid interference with extracellular melanin measurements ...
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Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles functionbioRxiv - Cell Biology 2024Quote: ... Cells were washed 3 times with PBS and incubated for 1 h with Alexa 488 nm conjugated secondary antibodies specific for mouse IgG (Invitrogen) diluted 1:2000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... The fins were then washed once with DPBS and the samples further sterilized for 2 h in antibiotic solution with Gentamicin (50ug/ml Gibco) and Primocin® (50ug/ml InvivoGen ...
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bioRxiv - Developmental Biology 2024Quote: ... The slides were washed three times with DPBS and then incubated for 1 h with fluorescence-conjugated secondary antibody (#A21206, Invitrogen) diluted 1:1000 in 5% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... the mix was incubated for 20 minutes at 37°C with 1 μl of RNase H from Invitrogen (18021-014). For LL3 gonads ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was changed at 24 h post-transduction and selected with puromycin dihydrochloride (5 μg/mL, A11138-03, Thermo Fisher) for two days ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used included Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody-HRP (Thermo Scientific, A27036, 1:2000) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... and Goat anti-rabbit IgG (H+L) Highly Cross Absorbed Secondary Antibody Alexa Fluor 488 (Invitrogen, A-11008, 1:500). Samples were also labeled with wheat germ agglutinin (WGA ...
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bioRxiv - Molecular Biology 2020Quote: ... BV2 were cultured at 37°C/5% CO2 in growth medium (Dulbecco’s modified Eagle’s medium (D-MEM, Gibco), supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Nvsix3/6:venus was generated by subcloning the Nvsix3/6 coding sequence into pENTR/D TOPO (ThermoFisher Scientific) using published primers previously used to PCR amplify Nvsix3/6 and synthesize Nvsix3/6 mRNA 47 ...
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bioRxiv - Neuroscience 2021Quote: ... Sorted nuclei were plated on poly-D-lysin coated glass Lab-Tek II chamber slide (Thermo Fisher Scientific) for confocal analysis.
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bioRxiv - Plant Biology 2020Quote: ... coding sequences of each gene were amplified by PCR and cloned into vector pENTR/D-TOPO (Life Technologies). These fragments were subsequently transferred to destination vectors using the Gateway system according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... HeLa cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, as received with L-Glutamine, 4.5 g/L D-glucose and pyruvate, Gibco) supplemented with FBS 5% (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... and plated on glass coverslips (Warner instruments; 64-0734 (CS-18R17)) coated with poly-D-lysine (Thermofisher; ICN10269491) and EHS laminin (Thermofisher ...
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bioRxiv - Neuroscience 2021Quote: ... the PCR product was cloned into pENTR using the pENTR/D-TOPO cloning kit (Thermo Fisher Cat #K240020) and the sequence confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2021Quote: COS7 cells were plated on poly(D-lysine)-coated coverslips and cultured in six-well plates (Thermo Scientific) for 48 hr ...
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bioRxiv - Plant Biology 2020Quote: ... and subsequently cloned into pENTR™ TOPO® vector(pENTR™/D-TOPO® Cloning Kits, ThermoFisher Scientific) to generate the corresponding entry clones ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were conjugated to 30 uL of Protein G Magnetic Beads (Dynabeads, Thermo Fisher Scientific 10-003-D), per ChIP ...
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bioRxiv - Neuroscience 2020Quote: ... EBs were then dissociated and 15,000 cells/well were plated on poly-D-Lysine/laminin (Thermo Fisher Scientific) -coated glass coverslips in 24-well plates ...
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bioRxiv - Cancer Biology 2021Quote: The siRNA duplex (21 nucleotides) against human cath-D siRNA (ID 4180) was purchased from Ambion (Austin, TX), and the firefly luciferase (Luc ...
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bioRxiv - Cell Biology 2020Quote: The mouse muscle cell line C2C1254 were cultured in growth media (Dulbecco’s Modified Eagle Medium (4.5 g/l D-Glucose, No L Glutamine, No Sodium Pyruvate (Gibco))+20% Fetal Bovine Solution-One Shot (Gibco)+1% Glut Max 100x (Gibco)) ...
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bioRxiv - Biophysics 2020Quote: ... 400 μl of ‘imaging DMEM’ ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 25 mM HEPES, [-] Sodium Pyruvate, Gibco) was added to an Eppendorf tube together with 3 μl and 7 μl of 1 μm and 4.95 μm VN coated beads ...
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bioRxiv - Cancer Biology 2022Quote: ... PANC-1 and HEK293T cells were maintained in DMEM with 4.5 g/l D-Glucose and GlutaMAX (Gibco), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.
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bioRxiv - Cell Biology 2022Quote: All cells were cultured and imaged in high-glucose DMEM (4.5 g/L D-glucose, 0.584 g/L L-glutamate, 110 mg/L sodium pyruvate; Gibco) supplemented with 10% fetal bovine serum (MedSupply Partners) ...
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bioRxiv - Microbiology 2022Quote: ... a mix of enzymes was used (collagenase V, Sigma, C9263-1G, 0.85 mg/ml; Collagenase D, Roche, 11088882001, 1.25 mg/ml; dispase, Gibco, 17105-041 ...
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bioRxiv - Cell Biology 2022Quote: ... Initial parathyrosphere cultures of seven days old (d) were dissociated to single cells using 0.05% trypsin-EDTA (Invitrogen), counted using Tryphan blue and cell concentration was adjusted to 2 x 106 cells per ml ...
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bioRxiv - Microbiology 2021Quote: Cell free glycosylation was conducted in S30 buffer with 0.1% n-dodecyl-β-d-maltopyranoside (DDM; Thermo Scientific) and 10 mM MnCl2 (Across Organics) ...
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bioRxiv - Developmental Biology 2020Quote: ... as directed by the manufacturer and employing oligo d(T)16 (for Fig. 1d & 4b) (Invitrogen™; N8080128) or random hexamers (for Fig ...
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bioRxiv - Neuroscience 2021Quote: ... 1.25 NaH2PO4, 26 NaHCO3 and 11 D–glucose (pH 7.35, B303 mOsm)) containing Alexa Flour 488 (50μ, Invitrogen) (tip opening ...
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bioRxiv - Plant Biology 2021Quote: ... the full length CDS sequence of EgSPCH was amplified and cloned into pENTR/D-TOPO (Thermo Fisher, USA), after which the entry clone was recombined into the destination vector pGWB541 (Nakagawa et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and cultured the following day on plates coated with poly-D-lysine and laminin in neurobasal medium (Invitrogen) supplemented with 2% B27 (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... can be measured in live cells by using 5-(pentafluorobenzoylamino) fluorescein Di-β-D-glucopyranoside (PFB-FDGlu; ThermoFisher) as a substrate that is taken up into acidic vesicles only ...