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Citations for Thermo Fisher :
4451 - 4500 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then rewashed 3X with TBS-T and then treated with secondary antibody Goat anti-Mouse IgG (H+L) HRP from (Invitrogen) diluted with blocking buffer for 1 h while shaking ...
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bioRxiv - Microbiology 2024Quote: ... and random primers (50℃ for 1 h in 20 μL reactions) with SuperScriptIII enzyme as recommended by the manufacturer (Invitrogen). The reaction mixes were then diluted 1:3 in nuclease-free water ...
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bioRxiv - Genomics 2024Quote: ... Single-stranded DNA FISH probes were then synthesized by Reverse-Transcription using the Maxima H Minus RT enzyme (ThermoFisher; EP0751). RT reactions (70 µL ...
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bioRxiv - Microbiology 2024Quote: 1 mL cells of Msm and Δwag31 either untreated or treated with 100 ng/mL ATc for 12 h were stained with 20 μg/mL BODIPY (Invitrogen). For examining lipids in Wag31 overexpression strain ...
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bioRxiv - Microbiology 2024Quote: ... Anti-mouse IgG (H+L) Alexa Flour 488 (Catalog no. A28175) and Ribogreen (Catalog no. R11490) were from Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Immunology 2024Quote: ... Sorted iNKT cells were cultured in cRPMI in 96-well plates that were previously coated for 12 h at 4 °C with 5 μg/mL of anti-CD3ε (clone 145-2C11, Invitrogen) in PBS ...
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bioRxiv - Immunology 2024Quote: ... stained slides were then washed in PBS for 5 min and incubated for 1 hour at room temperature with secondary Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (ThermoFisher), diluted 1:200 (v/v ...
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bioRxiv - Microbiology 2024Quote: ... All secondary antibodies goat anti-(human, mouse, rat, or rabbit) (H+L) used for immunofluorescence (1:1000) were purchased from Invitrogen. Secondary antibodies for western blotting ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.5% SDS) and incubated for 12-16 h at 4°C with 25 μL protein G Dynabeads (Invitrogen, 10004D) and 5 μg of antibody (Supplementary table 6) ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with an electrospray ionization source (H-ESI II Probe) coupled with an Ultimate 3000 RS HPLC (Thermo Fisher Scientific). Digested DNA was injected onto a Thermo Fisher Hypersil Gold aQ chromatography column (100 mm x 2.1 mm ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured 72 h later with the PrestoBlue HS cell viability reagent (#P50201, ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Reverse transcription was carried out with 1 μg RNA at 37 °C for 1 h with the following reagents: 5x reverse transcription buffer (Invitrogen), 0.1 M dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2024Quote: ... RNA released from the permeabilized tissues and captured by the DNB was reverse transcribed at 42 °C for 2 h using SuperScript II (Invitrogen, 18064-014 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed extensively in TBS before being incubated with biotinylated goat anti-rabbit IgG (H+L) secondary antibody (ThermoFisher Cat# B-2770 ...
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bioRxiv - Neuroscience 2024Quote: ... 1 h incubation with goat anti-rabbit IgG Alexa Fluor 546 secondary antibody (1:500, Life Technologies, Carlsbad, California, USA) were performed ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and goat anti-rabbit IgG (H + L) Alexa Fluor 647 secondary antibodies (1:200 in PBS; Cat. Nos. A11017 and A21245, Invitrogen) for 3 h ...
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bioRxiv - Bioengineering 2024Quote: ... After incubation with a similarly diluted alkaline phosphatase-conjugated goat anti-mouse IgG (H+L) secondary antibody (31346, Fisher Scientific), and washing steps ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... Fibroblasts were plated at a density of 20,000 cells/well in a 24 well plate and fixed after 24 h in 4% paraformaldehyde (PFA, 28908, Thermo Scientific) for 10 min at room temperature (RT) ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with ice-cold PBS and the primary antibody was detected using FITC-conjugated goat anti-mouse IgG (H+L) antibody (Invitrogen). Relative pre-TCR surface pre-TCR expression at each time point was calculated as the percentage of positive cells normalized to time 0.
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bioRxiv - Systems Biology 2024Quote: HAECs were plated at a density of 3 x 105 cells/well in 6-well format before being transfected with FOXO1 small interfering RNA (siRNA) or scramble siRNA (ON-TARGETplus, Dharmacon) (Table S3) for 4 h in Opti-MEM medium (Gibco) using Lipofectamine RNAiMAX transfection kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were produced according to Addgene’s protocol and 24-48 h post-infection cells were selected with 2 µg/ml puromycin (ThermoFisher Scientific) for 2-5 days ...
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bioRxiv - Immunology 2024Quote: Total RNA from cultured WT or Lmna-/--BMDCs (treated with LPS for 24 h) was isolated with TRI Reagent solution (Invitrogen) and isopropanol precipitation ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 1 h with a polyclonal Horseradish Peroxidase (HRP)-coupled anti-mouse IgG secondary antibody (Thermofisher Scientific) diluted 1:2000 in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... CAL-1 cells were infected with Salmonella for 4 h and then incubated for 30 min at 37 °C with CellROX Deep Red Reagent (1 µM, #C10422 Invitrogen Thermofisher) for total (cytoplasmic and nuclear ...
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bioRxiv - Immunology 2024Quote: ... and THP-1 cells were incubated with GZMK (50 nM) or C1s (50 nM) for 1 h on ice at 4 °C in HBSS with Ca2+ and Mg2+ (Gibco) containing 10 mM HEPES and 0.5% BSA (Millipore Sigma) ...
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bioRxiv - Microbiology 2024Quote: RNA Flow FISH analysis of unstimulated and 4HT-induced P3HR1-ZHT cells (24 and 48 h post-treatment) was performed using RNA PrimeFlow reagents (ThermoFisher RNA PrimeFlow Kit Catalog # ...
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bioRxiv - Microbiology 2024Quote: ... The plates were washed 3 times with PBS/Tween 20 0.05% (PBS-T) and saturated for 2 h at room temperature (RT) with 300 µl per well ELISA/ELISPOT diluent 1X (Invitrogen 00-4202-56 ...
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bioRxiv - Microbiology 2024Quote: ... Reporter replicon-transfected HEK293T cells were imaged 48 h after transfection on an EVOS M5000 Imaging System (Thermo Fisher Scientific) equipped with an RFP (531/593 nm ...
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bioRxiv - Microbiology 2024Quote: ... antibodies at a concentration of 20 µg/mL followed by goat anti-mouse IgG (H+L) AF488 secondary antibodies (Invitrogen). Data was acquired on a BD FACS Canto II and analyzed using FlowJo v10.7.1.
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bioRxiv - Neuroscience 2024Quote: ... and (2) Alexa Fluor Plus 488 Goat anti-Rabbit IgG (H+L) (for Calretinin stain; Catalog # A32731, Invitrogen; RRID: AB_2633280). Sections were then incubated for 10min in a 1:40,000 dilution of DAPI stock solution in PBT (5mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS/0.2% Tween with 10 μg/mL heparin (PTwH)/5% DMSO/3% donkey serum at 37°C for 7 d.The samples were then washed in PTwH for 24 h (5x) and incubated in donkey anti-rabbit Alexa647 (1:500, A31573; Invitrogen) in PTwH/3% donkey serum at 37°C for 7 d ...
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bioRxiv - Plant Biology 2024Quote: ... plants were washed twice in PBS containing 3% BSA and placed in the EdU click-IT reaction mixture with the 594 Alexa FLUOR fluorophore in the dark for 1 h (Invitrogen). This was followed by two washes with PBS with 3% BSA after which samples were placed in ClearSee solution for 1-4 days ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The slides were washed the next day and incubated with a secondary antibody (Invitrogen Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... coverslips were incubated in Goat anti-Rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor Plus 488-conjugated secondary antibody (Invitrogen) at a concentration of 1:1000 in Background Sniper for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then lysed at 48 h post-transfection in cold RIPA buffer supplemented with protease inhibitors (Thermofisher, catalog #: 89900). For Western blot analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h at room temperature and then with a secondary antibody Anti-Mouse Alexa 555 (A-21424, Thermo Fisher), as well as a Streptavidin/Alexa Fluor™ 488 conjugate recombinant protein (S11223 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells grown on 6-well plates were transiently transfected for 48 h with 1.5 μg of each plasmid DNA and 8 μl Lipofectamine 3000 (Invitrogen, #L3000001) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Blood was sampled at 6 h post-injection by submandibular bleed for serum quantification of IFN-α by ELISA (Invitrogen; BMS 6027 – analysed on TECAN Infinite F50 with Tecan i-control software ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in 1X PBS (3 x 10 m) before a 1 h incubation in Donkey anti-rabbit IgG (1:1000) (#A32754, Invitrogen) and Goat anti-chicken IgG (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were allowed to recover for 24 h and were subsequently transferred into 96-well plates containing media supplemented with 2 mg/mL G418 (#11811-031, Gibco). Next ...
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bioRxiv - Biochemistry 2024Quote: ... 70S ribosomes were isolated via centrifugation through a 0.29-1.17 M sucrose gradient at 29,000 rpm for 17 h in a TH-641 swing-out rotor (Thermo Fisher Scientific). The ribosomal fractions were withdrawn from the gradient ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation for 2 h at room temperature with goat Alexa Fluor 546-conjugated anti- mouse (Cat#A-11030; RRID:AB_2534089, Thermo Fisher Scientific) and Alexa Fluor 647-conjugated anti-rabbit secondary antibodies (Cat#A-21245 ...
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bioRxiv - Genomics 2024Quote: ... Samples were then incubated at 37 °C for 1 h after addition of 2 μl of RNase Cocktail Enzyme Mix (Ambion). Then 40 μg of Proteinase K (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... floating sections were incubated for 2 h at room temperature with the goat anti-mouse Alexa 488-labeled secondary antibody (1:500, Invitrogen). To visualize cell nuclei ...
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bioRxiv - Bioengineering 2024Quote: ... Purified DNA plasmids were eluted in ultrapure H 2 O and DNA concentration and purity was validated through A260 ratios on a NanoDrop Lite Spectrophotometer from ThermoFisher.
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Systems Biology 2024Quote: ... The islets were then incubated for 1h at 4 ℃ with Goat anti-Guinea pig immunoglobulin G (IgG) (H+L) secondary antibody (DyLightTM 488) (1:1000, SA5-10094, Invitrogen), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed in TBS-0.1% Tween-20 and then incubated in secondary antibodies (1:10,000, goat anti-rabbit IgG H+L secondary antibody HRP conjugate, 31460, Thermo Fisher) at room temperature for 1 hr ...
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bioRxiv - Bioengineering 2024Quote: ... samples were treated with Alexa Fluor 488 donkey anti-mouse IgG (H+L) secondary antibody (2 µg/mL, A-21202, Invitrogen) for 1 hour at RT and then washed again with PBS 1× ...