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Citations for Thermo Fisher :
4701 - 4750 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... U(H)PLC for method A was performed using a Dionex UltiMate 3000 rapid separation LC system (Thermo Fisher, http://www.thermofisher.com), combined with a Thermo Acclaim RSLC 120 C18 column (particle size 2.2 μm ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with an Alexa 594 Goat Anti-Rabbit IgG (H+L) (1:500) (Thermo Fisher Scientific, Waltham, MA, USA) and Alexa 488 Phalloidin (1:50 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesised from 1-5 μg of template RNA using he Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with MVA or MVAΔE5R at MOI 10 for 6 h in the presence of MG132 (25 μg/ml) and lysed in RIPA lysis buffer (ThermoFisher, 89901) on ice for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cells were centrifuged at 14,000 rpm for 1 h at 4°C to remove the cell debris and the supernatant was incubated to Ni2+-NTA resin (Thermo Fisher) pre equilibrated with lysis buffer ...
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bioRxiv - Genetics 2020Quote: ... then incubated with DAPI (1:5000) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibodies (1:200; Thermo Fisher). After incubation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 × 10 min with PBS and incubated for 1 h at room temperature with the secondary antibody (goat anti-rabbit Alexa Fluor 488, 1:1000; Invitrogen, Germany) diluted in blocking solution ...
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bioRxiv - Microbiology 2021Quote: ... cushion at 20,000 x g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (Thermo Fisher Scientific). Viral stock titers were titered on MRC-5 (TB40/EFb-eGFP ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed three times in PBS – 0.05% saponin and incubated for 2 h with a donkey anti-goat Alexa Fluor® 488 (1/1000, Invitrogen A11055) diluted in PBS – 0.05% saponin – 0.2% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells was acquired using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding an Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells were scanned using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... 500 ng of each RNA sample was used for retro-transcription using the RevertAid H Minus Reverse Transcriptase from Thermo Fisher. Quality of obtained cDNA extracts was verified by semi-quantitative RT-PCR using the reference gene PpAPT ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed 3 x 5 min with 500 mml per well of TBS and incubated at 37°C for 1h with 250ul of a mixture containing goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated with Alexa Fluor plus 488 (Invitrogen, USA) and goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 1 h (at RT with gentle shaking) in a blocking solution containing 10% normal goat serum (16210064, Life Technologies), 0.4% Triton X-100 and 0.1% glycine in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated goat anti-rabbit IgG F(ab’)2 and HRP-conjugated anti-mouse IgG(H+L) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 5 μM E64D) and incubated for 1 h in CCF2 solution (LB, CCF2-AM, Solution B [CCF2-AM kit K1032] Thermo Fisher) in the dark ...
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bioRxiv - Plant Biology 2021Quote: ... cDNAs were synthesized from 1 µg of total RNAs using oligo(dT) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Fisher Scientific). Quantitative PCR reactions were performed in a QuantStudio5 (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... bound antibody was detected by incubation for 1 h with Alexa Fluor 546-IgGs or Alexa Fluor 488-IgGs (1:200 for both, Invitrogen, USA).
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bioRxiv - Biochemistry 2021Quote: ... equal amounts of protein were submitted to Endo H treatment or mock-treated followed by SDS-PAGE analysis and transferred into a PVDF transfer membrane (ThermoFisher Scientific) as previously described (Duart et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were stained with Hematoxylin and Eosin (H&E) on an automated staining platform (HMS 740, Microm or Gemini AS, Thermo Scientific) or with Luxol Fast Blue - Cresyl Violet (LFBCV ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg RNA was used as a template for reverse transcription (RT) using the RevertAid H Minus First Strand cDNA Kit (Thermo Scientific). Sequence of primers used in this study is shown in Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then labeled at room temperature for 1 h with Alexa Fluor 488–conjugated goat anti-mouse secondary antibody (Molecular Probes), 0.2% saponin ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... LGm and OXPHOSm were plated in IBIDI μ-Slide 8 Well ibiTreat at a density of 6000 cells/well and after 24 h cells were incubated for 90 minutes with MitoTracker Red CMXRos (Molecular Probes, Invitrogen) at 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were again washed with Blocking Buffer twice for 1 h and mounted on microscope slides using ProLong Glass (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... with the appropriate secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Invitrogen, A23766) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.1% Tween-20 in 1X PBS pH 7.4 and then incubated overnight at 4°C with the appropriate secondary antibodies: Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 555 (Invitrogen, A32816) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... and were transfected with siRNAs at 50 nM for 48 h using the Lipofectamine™ RNAiMAX Reagent (13778-100, Invitrogen, USA).
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with V5-tagged WNT11 and 24 h post transfection proteins were crosslinked for 30 min with 1 mM DSS (Thermo Fisher) in 1 ml PBS + 1mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... were retrotranscribed from either 5’-CATGCTGCTGGTGGGTGTGCT-3’ or 5’-CCATAAAGCACCGGTGAGCAGAA-3’ endoglin specific reverse oligonucleotides (500 nM) using RevertAid H minus reverse Transcriptase (Thermo Scientific) 10 U/μl in 1X RevertAid H minus Buffer supplemented with 2 U/μl RNAse OUT (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was transferred to a 0.2 µm nitrocellulose membrane and blocked for 1 h at room temperature with SuperBlock™ blocking buffer (Thermo Scientific). Membranes were then washed four times in TBST buffer before incubation in a 1:5000 dilution of Monoclonal ANTI-FLAG® antibody (Monoclonal ANTI-FLAG® M2-Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 times for 5 min and incubated for 30 min with secondary antibodies diluted in PBT + 0.5% BSA: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-Rabbit IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... Fc and non-digested mAbs were removed from the flow-through by a 2 h incubation at RT with 200 µl of protein A resin per mg of initial mAb (Thermo Scientific). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were dry-mounted on slides using Fluoromount (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 1 h at room temperature with goat anti-guinea pig Alexa Fluor 647 secondary antibody (#A21450, Life Technologies). Sections were then incubated in the Click-IT reaction ...
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bioRxiv - Immunology 2022Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding an Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells were scanned using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... and co-transfected with wild type and mutant constructs of PKD 20 h post-seeding with 250 ng of wild-type and 250 ng mutant plasmid DNA per well using TurboFect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... the specimens were incubated for 1 hr at RT with anti-rabbit IgG(H+L)-Alexa 568-conjugated antibody (Donkey; Invitrogen, A10042), anti-mouse IgG2a-Alexa 488-conjugated antibody (Goat ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies). The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... cells were incubated with secondary antibodies: Alexa Fluor 488® F(ab’)2 fragment of goat anti-mouse IgG (H+L) (Invitrogen) and/or Alexa Fluor 594® F(ab’)2 fragment of goat anti-mouse IgG (H+L ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... the protein solution was dialyzed in buffer A for 3 h and passed through HisPur Ni-NTA resin (Thermo Fisher Scientific) to remove the 6×His tag ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg of total RNA was reverse transcribed using random primers and RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Scientific), following standard procedures ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed four times for 5 min with PBS and incubated for 2 h in the dark with Alexa Fluor 568-conjugated donkey anti-rat IgG (Life Technologies) at a dilution of 1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were added for 2 h at room temperature and the slides were washed as before and coverslipped with Prolong Gold anti-fade reagent (Molecular Probes). Most images were obtained with epifluorescent illumination and a 10× objective (Leica 760 or AF6000) ...
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bioRxiv - Molecular Biology 2022Quote: ... The secondary antibody was Alexa Fluor 549-conjugated goat anti-mouse IgG (H+L) (diluted 1:250 in PBST containing 5% goat serum, Invitrogen, USA) which was incubated overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 16 h and then the digest reaction was quenched with 1% formic acid (Fisher Scientific, Czech Republic). The resulting peptides were desalted using a C18 column (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from 400 ng of DNase-treated RNA using the RevertAid H Minus first-strand cDNA synthesis kit (Thermo Scientific), according to the manufacturer’s instructions for random hexamer primer (IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... sections were washed and incubated with for 1 h at room temperature with secondary antibodies goat anti-rabbit Alexa Flour 568 (1:1000, A11011; Life Technologies) for Nestin ...
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bioRxiv - Neuroscience 2022Quote: ... microglial cells and oligodendrocytes were removed by aspiration following 20 h shaking and the adherent cell population was collected after being detached using 0.05% trypsin-EDTA (15400054, Thermo Fisher Scientific). Collected cells were plated at 1 × 105 cells/cm2 density ...