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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 293 cells from a pMLINK tetracistronic vector (courtesy of Y. Shi, Tsinghua University, Beijing).45 HEK293 cells were cultured in Freestyle 293 media (Life Technologies), shaking at 125 rpm while incubating at 37 oC with 8% CO2 until a density 2 × 106 cells/ml was reached ...
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S gene containing plasmid p20017 and adenovirus backbone plasmid pAADV-C01 (Genemedi, China) were co-transfected into HEK293 based adapted viral production cell (ThermoFisher, USA). Viral production cells were seeded in a 6 well TC treated plate (Nest ...
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bioRxiv - Cancer Biology 2022Quote: ... LCV2-GFP or LCV2-RFP were mixed with sPAX2 and MD2.G plasmids and transfected in HEK293 cells using Lipofectamine 2000 (Life Technologies). After 24h ...
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bioRxiv - Developmental Biology 2024Quote: Cultured HEK293 cells were grown on 12 mm round cover glasses (Azer Scientific, #200121) pre-coated with poly-D-lysine (Gibco, #A3890401) in 24-well plates and then transfected with a plasmid encoding HA-tagged neurexin1 alpha using lipofectamine 2000 ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... A549 and the HEK293 cell line stably transduced with a firefly luciferase reporter under the control of a synthetic NF-κB promoter (HEK293-NF-κB_luc, BPS-Bioscience) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Cat# 61965-026) supplemented with 10 % fetal calf serum at 37 °C in a 5 % CO2 incubator.
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bioRxiv - Immunology 2022Quote: ... All soluble SOSIP Envs were expressed by transient transfection in HEK293-6E cells (National Research Council of Canada) or Expi293 cells (Life Technologies) and purified from transfected cell supernatants by 2G12 affinity chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... Constructs carrying the 3’UTRs were transiently co-transfected with vectors carrying Renilla-Luciferase and either miR-122 mimic (122-MIM) or scramble oligos (SCR) into HEK293 cells with Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: HEK293 cells were harvested and lysed in RIPA lysis with 1X Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). The concentration of total protein was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... and human embryonic kidney 293 cells (HEK293) were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Fisher Scientific, #MT10013CV) with 10% FBS at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 4 × 106 HEK293 Flp-In T-Rex cells with EGFP integrants were seeded in 15-cm dishes (Thermo Fisher Scientific) and incubated overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Culture inserts were removed 8h prior to imaging and HEK293 culture medium was changed to imaging medium – phenol red-free DMEM (Gibco, #31053028) with 10% FCS (Biosera ...
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bioRxiv - Cell Biology 2023Quote: ... Flp-In HEK293 cells were co-transfected with the pCDNA3-TurboID-cyclin F plasmid and the pOG44 Flp-Recombinase Expression Vector (Invitrogen, V600520) for co-expression of the Flp-recombinase using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Allantoic fluids with titre higher than 16 hemagglutination units were tested for bacterial contamination using Columbia sheep blood agar (Thermo Fisher, Germany). Virus stocks were dispensed in 2 mL-cryotubes and stored at −80°C until further use.
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bioRxiv - Physiology 2021Quote: ... on ice for 1 hour and followed by incubation with sheep anti-rat IgG Dynabeads M-450 (Invitrogen, Cat#11035, 50 μl) for 30 mins ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial stocks were maintained in 20% glycerol at −70°C and cultured on trypticase soy agar II with 5% sheep’s blood (SBA) (Fisher Scientific, Pittsburg, PA) at 35°C in ambient air for testing ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were incubated in primary antibodies (sheep anti-BPNT2, 1:1000, Invitrogen #PA5-47893; mouse anti-Actin, 1:1000, Invitrogen #MA1-744) overnight at 4C ...
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bioRxiv - Developmental Biology 2022Quote: ... REX1 antibody DA136 (3rd bleed) was diluted at 1:1000 and visualized using Donkey anti-Sheep HRP secondary antibody (Thermo Fisher Scientific). Immunoblots were imaged using enhanced chemiluminescence on a BioRad ChemiDoc imager (BioRad) ...
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bioRxiv - Microbiology 2019Quote: ... Strains were routinely grown at 37°C and 5% CO2 overnight on tryptic soy agar plates supplemented with 5% sheep blood (TSAII) (Thermo Fisher Scientific). Growth in broth culture was performed in BHI (Becton ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated in corresponding secondary antibodies (Alexa Fluor 488 Donkey anti-rabbit IgG 1:400, Life Technologies A-21206; Alexa Fluor 555 Donkey anti-sheep IgG 1:400, Life Technologies A-21099 ...
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Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotionbioRxiv - Neuroscience 2021Quote: ... Secondary Abs (Alexa 546 Donkey anti-Rabbit; Alexa 488 Donkey anti-goat, anti-mouse, or anti-sheep, all 1:400, Invitrogen, Oregon, USA) were diluted in a solution containing 1% donkey serum and PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were washed three times for 5 minutes in 0.05% PBS-Tween then incubated with appropriate secondary antibodies in the dark at room temperature (anti-IgG rabbit A488 and A647, anti-IgG mouse A555 and anti-IgG sheep A555, all from Thermo Fisher Scientific) and 1:1000 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by a donkey anti-sheep IgG coupled to horse radish peroxidase (R&D, MN) and developed with an ECL Western blotting kit (Thermo Scientific, NJ). The intensities of the cytochrome c bands were normalized to that of constitutively expressed β-actin and expressed as relative intensity ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used for immunocytochemistry were donkey anti-mouse/rabbit/goat/sheep conjugated to Alexa Fluor 488/546/555/647 (Invitrogen 1:500), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Microbiology 2023Quote: For routine cultivation, all Streptococci were grown for 24 – 48 hours on Columbia agar (Oxoid, UK) with 5% defibrinated sheep blood (Thermo Scientific, USA) at 37°C in presence of 5% CO2 and 95 % relative humidity or in a vinyl anaerobic chamber with < 5 ppm O2 (Coy labs ...
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bioRxiv - Microbiology 2024Quote: ... and also cultured16 the fluid by spread-plating 20 microliters onto a pre-reduced tryptic soy 5% defibrinated sheep blood agar (80% N–10% H– 10% CO2, at 37°C; Thermo Fisher Scientific) using a variable-atmosphere anaerobic Whitley workstation A85 (540 plate capacity ...
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bioRxiv - Neuroscience 2023Quote: ... together at 4°C for 18 hours followed by appropriate secondary antibodies (donkey anti-mouse IgG [H+L]; A48257 and donkey anti-sheep IgG [H+L]; A11016; 1:1000; Invitrogen, Waltham, MA) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... goat anti-mouse or donkey anti-sheep IgG secondaries linked to Alexa Fluor 488 (1:500, Thermo Fisher #A-11034, #A-11001), Alexa Fluor 546 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by secondary antibody for 30 minutes (either Alexa-Fluor 488 or 594 conjugated goat anti-mouse, goat anti-rabbit, or goat anti-sheep, all at 1:200, Thermo Fisher Scientific). After nuclear staining with DAPI (4’,6-diamidino-2-phenylindole) ...
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bioRxiv - Microbiology 2023Quote: ... generated as described in [16]) was used at 1:200 (Table 1) before staining with Donkey anti-Sheep AF488 Secondary Antibody (Invitrogen, A-11015) at 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections and incubated them in PBST containing the corresponding secondary antibody tagged with fluorophores that match our microscope filters (1:300, Alexafluor 488 goat anti-rabbit, A11008, Lot # 2557379, Alexafluor 555 donkey anti-sheep, A 21436, Lot # 2420712 ThermoFisher Scientific, US), 1% NGS and 1% NDS ...
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bioRxiv - Microbiology 2020Quote: ... Table S1) were performed using an automated carbonate preparation device (GasBench II) connected to a Delta V Advantage (Thermo Fisher Scientific Inc.) isotopic ratio mass spectrometer at the Earth Sciences Department ...
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bioRxiv - Plant Biology 2020Quote: ... the MSL10 gene was amplified from genomic wild-type Col-0 DNA using gene-specific primers listed in Table S1 and cloned into pENTR/D-TOPO entry vector (Thermo Fisher Scientific), then recombined into pBGW destination vectors (Karimi et al. ...
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bioRxiv - Biochemistry 2020Quote: All chemically synthesized 41-mer TOD-S1 RNA molecules were generated at 40 nmol scale using the ABI-394 DNA/RNA synthesizer (Thermo Fisher Scientific) at the Stanford Protein and Nucleic Acid Facility (PAN) ...
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bioRxiv - Bioengineering 2020Quote: ... sequenced (Fig. S1) and recombined into pK7WGR2 (Vlaams Institute for Biotechnology) using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific). AGPAT was cloned in the pBinGlyRed3 vector in between the soybean glycinin-1 (GLY ...
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bioRxiv - Cell Biology 2021Quote: The SFI1 antibody was raised in rabbit against the GST-fused C-terminal domain of SFI1 (aa1021 to aa1240) (Figure S1) and affinity-purified on AminoLink® Coupling Resin (20381 Thermo Fisher) coupled to the MBP-fused C-terminal domain (using the same aa sequence).
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bioRxiv - Plant Biology 2021Quote: ... The coding sequence of OsSWEET11b was PCR amplified without STOP codon (primers: Table S1) using Phusion High-Fidelity PCR polymerase (Thermo Fisher Scientific) and cloned into the Gateway donor vector pDONR221 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... A 280 bp DNA segment was also amplified using the RNAi-1549-F and RNAi-1836-R primers (Supplementary Table S1) and cloned into the pHELLSGATE12 plasmid using the Gateway system (Invitrogen, CA, USA) to generate 35S::YFT1-RNAi (referred to as YFT1-RNAi) ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then stained with 20 µg/mL biotinylated S protein (S1/S2) or 1:1000 Anti-HS (Clone F58-10E4) (Fisher Scientific, NC1183789) in PBS containing 0.5% BSA ...
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bioRxiv - Genetics 2022Quote: ... cells were transfected with 50nm siRNA CTRL or siRNA GLB1 (IDT, sequence available in Table S1) with lipofectamine RNAimax (Thermo fisher scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... H9c2 cells or CFs were transfected with 1µg/ml of MYOCD siRNA (Table S1 in supplementary data) which targets both the cardiac isoforms (Silencer® Select pre-designed MYOCD siRNA; ID s140959; Ambion, USA). Cells transfected using scrambled sequence anti-miR (Ambion ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem cells and human induced pluripotent stem cells (hESCs and iPSCs, Table S1) were grown using StemFlex™ Medium (A3349401, Gibco™) supplemented with Normocin™ Antimicrobial Reagent (Invivogen ...
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bioRxiv - Cell Biology 2021Quote: ... was used for cDNA synthesis and real-time PCR using TaqMan probes (Table S1) on an Applied Biosystems 7500-Fast real-time PCR System (Thermo Fisher Scientific). All real-time PCR reactions were performed in triplicate and the average of Ct values normalized to the geometric mean of ACTB and GAPDH as endogenous control genes ...
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bioRxiv - Bioengineering 2020Quote: Plant produced S1 domain variants (glycoslated and deglycosylated variants) were purified using HisPur™ Ni-NTA Resin (ThermoFisher scientific, Cat. No. 8822) as described previously19 ...
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bioRxiv - Microbiology 2020Quote: ... and from original strains (C2865, C2853, C2854, C2855) were digested with 1 Unit of S1 enzyme (1 min at 37°C) (Thermo Fisher Scientific) for plasmid linearization ...
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bioRxiv - Microbiology 2020Quote: ... the recombination substrate was a synthesized modified protein V DNA sequence in which all lysine codons were replaced by arginine codons flanked the by left and right homology arms (Table S1; synthesized by Thermo Fisher Scientific). The resulting AdV-C5-V-KR bacmid then served as the template for constructing the AdV-C5-V-KRrev mutant ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once before being stained with surface mAb (Table S1) and viability dye LIVE/DEAD Fixable Near-IR (Thermo-Fisher Scientific, USA) for 30 min at 4°C ...