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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and lines were genotyped using primers listed in Table S1 and Phusion High-Fidelity PCR Master Mix (with GC Buffer) (Thermo Scientific, F532). PCR products of candidate clonal lines showing predicted PCR band sizes in agarose gel electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: All hESCs and hiPSCs (see Table S1) were maintained under feeder-free conditions in Essential 8 (E8) cell culture medium (Thermo Fisher Scientific) and vitronectin (VTN-N ...
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bioRxiv - Cancer Biology 2024Quote: ... S1 fraction was washed from the filter using fresh full media supplemented with 100 U/mL collagenase type IV (Life Technologies, 17104019), and 15 mM HEPES (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... and the mutated APLFR37A along with their respective vector controls (Table S1) were ectopically expressed in E14 ESCs using lipofectamine 2000 reagent (Invitrogen #11668-019), according to the earlier reports (Majumder et al. ...
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bioRxiv - Plant Biology 2023Quote: ... WRKY60 (1649 bp) and WRKY72 (1623 bp) with the primers listed in Table S1 using Phusion™ High-Fidelity DNA polymerase (Thermo Scientific). PCR products were A-tailed with Taq polymerase and cloned into pCR8/GW/TOPO (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... the full-length MpARK was amplified from Tak-1 cDNA by PCR using KOD plus (TOYOBO) with the primers in Table S1 and cloned into pENTR/D-TOPO cloning vector (Thermo Fisher Scientific). This vector was confirmed by sequencing and designated as pENTR/D-TOPO-MpARK ...
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bioRxiv - Immunology 2023Quote: ... in order to conduct real-time PCR using the indicated primer pairs from PrimerBank (24) (Table S1) with SYBR Green master mix (ThermoFisher, Waltham MA).
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bioRxiv - Cell Biology 2023Quote: ... 1 and S1) was performed on a Tecnai 20 equipped with a field emission gun and operated at 200 kV (Thermo Fisher company). Images were recorded using SerialEM software on a Falcon II (FEI ...
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bioRxiv - Cancer Biology 2023Quote: ... as a template and gene-specific PCR primers (Supplementary Table S1) mixed with Power SYBR Green Master Mix (4367659, Thermo Fisher Scientific) using a Quant Studio 3 thermocycler (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... A 5’ universal sequence and a 5’ amino modifier C6 (5AmMC6, to prevent unbarcoded amplicons from being sequenced) were added to each primer (Supplementary Table S1, Thermo Fisher Scientific) for the first-round L-PCR.
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bioRxiv - Neuroscience 2024Quote: NeuN IF in the S1 were used to assess off-targeting binding of a Dk anti-Ms IgG (H+L) secondary Ab (A21202, Thermo Fisher Scientific) and a Dk anti-Rb IgG (H+L ...
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bioRxiv - Pathology 2024Quote: ... 3 µL of diluted cDNA diluted was added to 1 µL of each primer (S1 Table) with 10 µM and 5 µL of SYBR Green Master Mix (Applied Biosystems, Life Technologies), using the following thermal program ...
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bioRxiv - Cell Biology 2020Quote: The V5-tag antibody conjugated with FITC (catalog # R963-25) was from Invitrogen (Carlsbad, CA). Trypsin (catalog # V511A ...
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bioRxiv - Molecular Biology 2021Quote: ... western blot and detection of myc tag using anti-myc monoclonal 9E10 (Thermo Fisher Scientific) and secondary antibodies anti-mouse-HRP (Cat# sc-516102 ...
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bioRxiv - Immunology 2022Quote: ... peptides from the lung homogenates were labeled with a Tandem Mass Tag (TMT; Thermo Fisher) and mixed in equal proportions ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were prepared and TMT-labeled per manufacturer’s protocol (ThermoFisher TMT10plex Mass Tag Labeling Kits). Following TMT labeling ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then labeled using 6-plex tandem mass tags (TMT; Thermo Scientific, Waltham MA) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... an HA tag was added to IQGAP1 and cloned via Gateway PCR (Invitrogen, Carlsbad, CA) into a pT3-EF5α vector ...
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bioRxiv - Systems Biology 2021Quote: ... trypsinized peptide samples were subsequently labeled with isobaric tags (TMT 6-plex, Thermo Fisher Scientific). All samples were labelled with individual tags ...
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bioRxiv - Molecular Biology 2021Quote: ... The primary antibody against the V5 tag on FRQ was sourced from Invitrogen (46-1157) and used at a 1:5000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... each fused to a C-terminal HA-tag using lipofectamine 3000 transfection reagent (ThermoFisher, L3000015) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... EVs were detected using a 1:100 anti-GFP Tag antibody (Thermo Fisher Scientific, #GF28R).
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bioRxiv - Immunology 2022Quote: ... MYC-tag based positive selection was performed according to the manufacturer’s instructions (Thermo Fisher, 88843).
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bioRxiv - Immunology 2022Quote: ... MYC-tag based positive selection was performed according to the manufacturer’s protocol (Thermo Fisher, 88843).
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bioRxiv - Biochemistry 2022Quote: ... 1024-1090 were cloned with an N-terminal 6XHis tag into the pFastBac1 vector (Invitrogen) for insect cell expression ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... then incubated in a 200 µg/ml solution of anti-6xHis-tag antibody (Thermo Fisher) overnight under constant agitation ...
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bioRxiv - Neuroscience 2020Quote: Tandem mass tag (TMT) labeling was performed according to manufacturer’s instructions (# A44520 Thermo Fisher Scientific). In brief ...
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bioRxiv - Biochemistry 2020Quote: ... Cdc50 proteins fused to the 10xHis tag were detected using the HisProbeTM-HRP conjugate (ThermoFisher) diluted to 1:2000 in 2 % (w/v ...
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bioRxiv - Biophysics 2021Quote: ... and 6xHis tags Recombinant baculoviruses were generated using the Bac-to-Bac system (Thermo Fisher). High Five cells (Thermo Fisher B85502 ...
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bioRxiv - Microbiology 2021Quote: ... The his-tag was cleaved using AcTEV protease according to manufacturer’s instructions (Invitrogen, Carlsbad, CA), and the his-tagged protease and noncleaved uSpike protein were removed by binding to Ni-NTA resin ...
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bioRxiv - Genomics 2022Quote: ... Amine-modified oligo tags were conjugated to antibodies using modifications of Site-Click chemistry (Invitrogen) and heterobifunctional DBCO linker (Click Chemistry Tools) ...
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bioRxiv - Neuroscience 2022Quote: ... Digested peptides were labeled with 16-plex TMT isobaric mass tag reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The GST tag of the VP8* protein was removed using the thrombin (Thermo Fisher Scientific) after dialysis into the buffer (20 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2019Quote: ... PpdD-HA pilin was detected with an anti-HA antibody (HA Tag Polyclonal Antibody, Invitrogen). The G ...
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bioRxiv - Pathology 2020Quote: ... The 10 different tandem mass tag labels comprising the TMT10plex™ kit (Thermo Fisher Scientific) were dissolved in 41 μL anhydrous acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with Alexa Fluor 488–conjugated 6x-His Tag monoclonal antibody (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells were transfected with the corresponding FLAG tag expression constructs using Turbofect (ThermoFisher Scientific). The whole cell extracts were prepared as described in 2.6 ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Genetics 2020Quote: ... and labeled by tandem mass tag isotopes (TMT10plex Isobaric Label Reagent Set, Thermo Scientific 90110). All the tagged samples were pooled ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA coated magnetic beads (Dynabeads™ His-Tag Isolation and Pulldown from Invitrogen, USA) were added to the solution and incubated for 10 additional min in order to bind the his-tagged TDF and PKnG proteins ...
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bioRxiv - Neuroscience 2020Quote: ... Proteolytic peptides were labeled using a tandem-mass-tag (TMT)- 10plex reagent set (Thermo Scientific) according to the manufacturer’s protocol and stored at −80 °C until further use ...
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bioRxiv - Systems Biology 2019Quote: ... The His tag was cleaved by overnight incubation at 4 °C with SUMO protease (Invitrogen), after which the sample was loaded again onto a HisTrap FF column to recover the cleaved products ...
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bioRxiv - Developmental Biology 2020Quote: ... and verified by Western blot with primary antibodies against 6x His tag (Invitrogen, MA1-21315), and DLX1 (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... they were isotopically labeled using the 10-plex tandem mass tag (TMT, Thermo Fisher Scientific), as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the constructs were all based on a pcDNA3.1 vector containing a V5/His-tag (Invitrogen). Untagged constructs for comparison were prepared by introducing a stop codon into the pcDNA3.1/V5-His constructs using the same site-directed mutagenesis kit as above ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 μl of Dynabeads™ His-Tag Isolation and Pulldown magnetic beads (ThermoFisher Scientific) were added into each well ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-GFP HRP conjugated GFP tag ( 1:1000, Cat #HRP-66002, ThermoFisher, Waltham, MA), mouse anti-Prom1 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% Tween-20) and added to 20 μl Dynabead His-Tag beads (ThermoFisher Scientific; 10103D). After incubation on a roller at 4°C for 1 h ...