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Citations for Thermo Fisher :
4501 - 4550 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... followed by probing with secondary antibodies (goat anti-rabbit Alexafluor 488, A11034, 1:500; goat anti-rat Alexafluor 647, A21247, 1:500, ThermoFisher Scientific) applied and washed as per the primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: Cell sizes of mouse and rat embryonic molars were determined by staining 6 µm thick histological sections with DiI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Immunology 2022Quote: ... Control APC755 tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (ThermoFisher Scientific) and incubated at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with secondary antibodies of goat-anti-rat 488 and goat-anti-rabbit 594 (1:500, Invitrogen, A11006, A11037) 1.5 hours in room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Wings were then incubated in 1:500 secondary antibody at the concentration 1:500 with anti-rat AF488 (Invitrogen, #A-11006) and anti-Guinea pig AF555 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The surface of the gel was then rendered permissive to cell adhesion by coating Rat Tail collagen I (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded samples were then combined and blocked for 15 min at 4°C with 100 μl of mouse and rat serum (both from Thermo Fisher) diluted at a 1:33 (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 times with PBS and incubated in secondary Alexa Fluor 555 conjugated goat anti-rat (Invitrogen, A-21434) and secondary Alexa Fluor 488 conjugated goat anti-rabbit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the coverslips were subjected to staining using the following secondary antibodies: goat anti-rat Alexa Fluor 594 (Thermo Scientific, #A-11007), goat anti-mouse Alexa Fluor 594 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Incorporation of CldU was detected using rat-anti-BrdU antibodies (1:500; BU1/75, AbD Serotec) and Alexa fluor-555-labelled goat-anti-rat antibodies (1:500; Molecular Probes), whereas incorporated IdU was detected using mouse-anti-BrdU antibodies (1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in DPBS for 10 min and incubated with secondary antibodies (Donkey anti-rabbit Life Technologies cat. no A31573, 1:400; Donkey anti-rat ThermoFisher Scientific cat ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies were used in the following order and concentrations: BrdU (BU1/75) rat monoclonal antibody (MA1-82088; ThermoFisher; 1:100) and Chicken anti-rat Alexa Fluor 488 (A21470 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
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bioRxiv - Bioengineering 2024Quote: ... and then stained for 1 h at room temperature in a humidified chamber with AlexaFluor-488-labeled donkey polyclonal anti-rat IgG (1:500, Invitrogen, A21208) and AlexaFluor-555-labeled donkey polyclonal anti-rabbit IgG (1:500 ...
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bioRxiv - Plant Biology 2024Quote: ... Immunolabelling experiments were performed with LM19 and LM20 (1:10 dilution in TTBS-milk, 2 h incubation) followed by anti-rat IgG-Alexa488 (Invitrogen®) or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438 ...
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bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
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bioRxiv - Cell Biology 2024Quote: The hippocampus was dissected from TP18-day Sprague Dawley rat fetuses of either sex using cold dissection medium L-15 (Gibco, 21083027). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used were conjugated Alexa Fluor 488 or 647 against rat or rabbit (ThermoFisher, A11008, A11006, A21245, A21247; 1:500) and conjugated Alexa Fluor 488 against chicken (Abcam ...
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bioRxiv - Genomics 2024Quote: ... and incubated with either anti-rabbit or anti-rat secondary antibodies with AF594 (diluted 1:400, A11012 or A1107, Thermofisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... we cultivated the corresponding rat hybridomas in IMDM (Gibco, 12440-053; supplemented with 10 % FCS, 1X MEM Non-essential amino acid solution (ThermoFisher, 11140050), 2 mM Glutamax (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were as follows: isotype-specific Alexa Fluor 488- and Alexa Fluor 546- conjugated goat anti-rat IgG antibodies (Thermo Fisher Scientific Cat# A-21434 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1h at RT and stained o/n with antibody fluorescent conjugates (1:500, anti-mouse-AF488, anti-rabbit-AF555 or 647, anti-rat- AF546, all from Thermo-Fisher Scientific), 1mg/ml DAPI solution 1:500 and PNA-lectin AF594 1:1000 (1mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Lung cell suspension was then subjected to positive sorting with an antibody against the endothelial cell marker ICAM-2 conjugated to magnetic Dynabeads Sheep Anti-Rat IgG (11035) from Thermo Fisher, for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... we counted the number of TH+/PHOX2B+ and TH+/PHOX2B- cells along the ventrolateral medulla (8 sections/rat; 240 µm interval) using an EVOS fluorescent microscope (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 565 (1:500, anti-rat coupled to Alexa Fluor 546, Cat No. A11081, Fisher Scientific, Waltham, MA, USA) secondary antibodies diluted in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rat Alexa 488 Invitrogen A11006; 1:500, goat anti-rabbit Alexa 555 Invitrogen A21429; 1:500 streptavidin Alexa 647 Invitrogen S32357). Washes in PBS with 0.2% Triton were performed three times at 5 ...
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bioRxiv - Plant Biology 2024Quote: ... and horseradish peroxidase-labeled goat anti-rat as the secondary antibody at a dilution of 1:5000 (Invitrogen, catalog no. 31470).
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bioRxiv - Pathology 2024Quote: ... tissues were washed in PBS and probed using a goat-anti-rat IgG 488 secondary antibody (Invitrogen A-11006, 1:500) for one hour ...
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bioRxiv - Molecular Biology 2024Quote: 12mm diameter coverslips in 24 well plate were coated with 50µg/ml rat tail Collagen I (Gibco, Catalog number: A10483-01) diluted in 0.02M acetic acid for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times for 15 min in PBS before a 2 h incubation with either a donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (dilution 1:500, Invitrogen, #A21208) or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 500 μl rat tail collagen I (50 μg/ml diluted in 25 mM acetic acid, Thermo Fisher Scientific, catalog no. A1048301) was added to each well for one hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from rats’ hearts with use of the Gene Jet PCR Purification Kit (Thermo Fisher Scientific, Waltham, MA) and quantified with a Nano-Drop One spectrophotometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... the dissected tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: Hippocampal neurons were isolated from the Hippocampi of embryonic day 17–18 Sprague Dawley rats by dissociating in TrypLE™ Select Enzyme (Gibco) supplemented with 0.005% DNaseI in an incubator maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from 100-150 mg of flash-frozen rat somatosensory cortex tissue using TRIzol reagent (ThermoFisher Scientific #15596026) as previously described (Rio et al. ...
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bioRxiv - Biophysics 2021Quote: ... Cultured cells were stained with mAb 0614 or human chimera MAb 0614-5 as the primary antibody and Alexa Fluor 488– or 647–conjugated anti-rat or human-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... and secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 633-conjugated goat anti-rat IgG were from Invitrogen (Carlsbad, CA). Vibrant DiD membrane-labeling reagent was from Thermo Fisher (Waltham ...
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bioRxiv - Cell Biology 2020Quote: For live-cell imaging of neurons, primary cultures of rat cortical neurons were prepared as described (Govind et al., 2012) using Neurobasal Medium (Thermo Fisher; 21103049), 2% (v/v ...
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bioRxiv - Genetics 2021Quote: ... in PBS-T and subsequently incubated for one hour with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG secondary antibody at 1:20,000 (Invitrogen cat. number 31470). Blots were then rinsed again once quickly ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies include: goat-anti-rat polyclonal Alexa Fluor and goat-anti-mouse polyclonal Alexa Fluor 488 (both 1:1000, Thermofisher, Waltham, MA).
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bioRxiv - Neuroscience 2022Quote: ... counted and re-seeded on poly-D-Lysine coated dishes containing Rat astrocytes in neuronal medium (B27 Electrophysiology medium (Gibco, A14137-01) supplemented with 1× MEM non-essential amino acids ...
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bioRxiv - Molecular Biology 2020Quote: ... the slides were incubated with the following secondary antibodies (anti-rat IgG Alexa Fluor 594, Molecular Probes A-11007; anti-mouse IgG1 Alexa Fluor 488, Molecular Probes A-21121 ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa Fluor 488-conjugated goat anti-rat IgG (H + L) antibodies and Alexa Fluor 594-conjugated goat anti-mouse IgG (H + L) antibodies (ThermoFisher Scientific, A11005) were used as secondary antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and then the corresponding secondary antibodies: anti-rat IgG (Alexa Fluor 488 conjugated, Molecular Probes A21210 and Dylight 550 Invitrogen SA5-10027) and IgM (Dylight 488 conjugated ...