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Citations for Thermo Fisher :
4351 - 4400 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... A plasmid was constructed utilizing the Gateway LR Clonease II Plus system (ThermoFisher). To create uas:cas9mkate-u6:piezo1gRNA ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of plasmid were transfected into the cells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 0.5µg Plasmid DNA and 2µM siRNA were diluted in Optimem (3198-062, Gibco) and mixed ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were transfected with plasmids using Lipofectamine 3000 (Invitrogen cat no. 3000008) according to the manufacturer’s instructions with a slight modification ...
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bioRxiv - Genetics 2023Quote: ... Each enhancer candidate was TOPO-cloned into the pENTR5′-TOPO plasmid (K59120, Invitrogen) using halt-volume reactions according to the manufacturer’s instructions (half-volume reactions) ...
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bioRxiv - Developmental Biology 2023Quote: ... Assembled Cas9/sgRNA plasmids were transfected into XEN cells using Lipofectamine 3000 (Invitrogen). E2-Crimson-positive cells were assessed for successful knockout of gene expression and reprogramming potential (see Figure 5B-C and S6C) ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmids were transfected into the cells using Opti-MEM (Thermo Fisher Scientific) and Viofectin Transfection Reagent (Viogene ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected when needed with 15 μg plasmid using Lipofectamine 2000 (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and the linear plasmid was ligated using T4 DNA ligase (Thermo Scientific™) following manufacturer protocols ...
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bioRxiv - Microbiology 2023Quote: ... A pCR 4-TOPO or pCR- XL-2-TOPO plasmid (ThermoFisher, Waltham, Massachusetts) with an insert spanning ITS1-5.8S rRNA-ITS2-D1/D2 region of 28S rRNA from a pure culture strain was used as a positive control ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were cotransfected at DIV14 with plasmids by using Lipofectamine 2000 reagent (Invitrogen). Cells were fixed at DIV21 with 4% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... the cloning vector pKNG101 plasmid was amplified using Phusion Master Mix (Thermo Scientific) and digested by DpnI (NEB BioEngland ...
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bioRxiv - Plant Biology 2023Quote: ... The obtained plasmids were recombined by an LR clonase II (Thermo Fisher Scientific) into the pXCSG-mYFP vector with a C-terminally fused mYFP epitope tag ...
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bioRxiv - Microbiology 2023Quote: ... 62–64 by transient plasmid cotransfection in the Expi293 Expression System (Thermo Fisher) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were heat transformed into subcloning efficiency chemically competent DH5α cells (Thermofisher, USA) and purified using endotoxin-free maxiprep kits (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: Plasmid transfection was performed using the Lipofectamine 3000 reagent (Invitrogen, ref. L3000-008) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... run on 1% agarose gels and recovered using GeneJET Plasmid miniprep kit (ThermoFisher). Recovered fragments were assembled using NEBuilder kit (NEB) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were cloned by Gibson isothermal assembly and propagated in DB3.1 cells (Invitrogen). We also generated a series of constitutive lentiviral plasmids as part of this work pLV-EgI-BlastR (EF1a-Gateway-IRES-BlastR) ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transfected into cells using lipofectamine 2000 and Plus reagent (Invitrogen) and incubated for 6 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Plasmid pBH-vsx2-memCerulean-pA was made using the Gateway system (ThermoFisher, 12538120) with combinations of entry and destination plasmids as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of plasmids and siRNA was performed using Lipofectamine LTX (Thermo Fisher Scientific) and Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... cells were transfected with 500 ng of each plasmid via lipofectamine 2000 (Invitrogen) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The amplified linear plasmid backbone was DpnI-digested with Fastdigest DpnI (Thermo Fisher) to remove residual template DNA prior to the SLiCE reaction ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 µg plasmid DNA were added to 50 µl OptiMEM (Thermo Fisher) and another 50 µl PEI mix (OptiMEM with double the amount of PEI than DNA ...
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bioRxiv - Cell Biology 2023Quote: Stable lines were obtained by cotransfecting the plasmid of interest with pCoHygro (Invitrogen) or pCoPuro (Addgene plasmid #17533 ...
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bioRxiv - Bioengineering 2023Quote: ... and the mini-donor plasmid were combined with Lipofectamine Stem Transfection Reagent (ThermoFisher) (1:4 ratio ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected into NIH-3T3 cells using Lipofectamine 3000 reagent (L3000015, Invitrogen). The final concentration of plasmids was 1 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... the lentiviral plasmids were added to the cells with Lipofectamine 2000 (ThermoFisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... IgH and IgL plasmids were co-transfected using polyethyleneimine into 293FreeStyle cells (Invitrogen). Supernatants were screened for anti-HLA reactivity using HLA cytometric bead arrays described below ...
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bioRxiv - Biochemistry 2023Quote: ... coli was purified using the GenJET Plasmid Miniprep kit (Thermo Scientific, Waltham, USA). Chromosomal DNA from Hm ...
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bioRxiv - Biophysics 2023Quote: ... and 1 µg of plasmid per well in Opti-MEM (Thermo Fisher Scientific), supplemented with 1 µM of all-trans-retinal (Sigma) ...
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bioRxiv - Biophysics 2024Quote: GAF-DBD (Table 1) was cloned into pET SUMO plasmid (Invitrogen K300-01) using Gibson Assembly (NEB E5520S ...
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bioRxiv - Biophysics 2024Quote: ... samples were transfected with corresponding plasmids using Lipofectamine 3000 (Invitrogen, cat. no. L3000150) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Transfection of synTF plasmid constructs was perfored using Lipofectamine 3000 (Thermo Fisher Scientific). 500,000 cells were plated in 6 cm culture plates and transfected the following day with the corresponding ng of synTF plus single stranded filler DNA (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... DNA from colonies was isolated with the PureLink Quick Plasmid Miniprep Kit (Invitrogen). DNA was sequenced with the M13 Forward primer.
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bioRxiv - Biophysics 2024Quote: ... MDCK cells were transfected with respective plasmid DNA using Lipofectamine 2000 (Invitrogen, 11668019). Appropriate selection for positives was done by supplementing culture medium with 800 μg ml−1 Geneticin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 30-100 ng of plasmid DNA was transfected using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s instructions for 5-8 hours ...
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bioRxiv - Cell Biology 2024Quote: ... N2a cell transfections involved purified plasmids and lipofectamine 3000 (Invitrogen, Waltham, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Plasmid inserts were excised by restriction digest and recovered with SizeSelect gels (Invitrogen) before being used to template Poly A tail PCRs ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid constructs were diluted in OPTI-MEM medium (Life Technologies, Waltham, MA, US) and transfections were performed using Lipofectamine 3000 Transfection Reagent (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Huh7OK1 cells were transfected with these plasmids using Lipofectamine LTX (Thermo Fisher Scientific). GFP-positive cells were isolated using a FACSAriaTM cell sorter (BD Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... both ATG-mutated plasmids were transformed into MAX Efficiency Stbl2 Competent Cells (Invitrogen). Clones were screened by restriction enzyme digestion and verified by Sanger sequencing using F primers in the pKL backbone upstream of RLBP1 and in exons 5 and 7 of RLBP1 ...
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bioRxiv - Genomics 2024Quote: ... We used packaging plasmids (pLP1, pLP2, and pLP/VSV-G; all from Invitrogen) and pLVX-Luc-IRES-ZsGreen1_ Cat ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with 500 ng plasmid DNA using Lipofectamine 2000 (Invitrogen) with a DNA:Lipofectamine ratio of 1:6 according to the manufacturer’s protocol ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... PCR products were cloned into the pCR8/GW/TOPO TA plasmid (Thermo Fisher) and then shuttled into a Gateway-converted pCDNA3.1 mammalian expression vector (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... competent cells and further recovered using GeneJET Plasmid Maxiprep Kit (ThermoFisher Scientific, Canada) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... then the plasmid was digested by BamHI-NotI 10U/μL (Thermo Fisher Scientific) restriction enzymes in Fast Digest Buffer for 1hour at 37°C to purify the transgene of interest ...
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bioRxiv - Biochemistry 2024Quote: ... the successful plasmid DNA was transformed into the INVSc1 yeast (Thermo Fisher Scientific) using S.c ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution, Molecular Probes - cat. A21210) and 2 μg/mL of DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... 1:500 (all from the Developmental Studies Hybridoma Bank and anti-mouse and anti-rat IgG Alexa Fluors 568 and 647, from Molecular Probes); some secondary antibodies were preabsorbed with fixed y w embryos ...