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Citations for Thermo Fisher :
4601 - 4650 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... at 4°C and bound antibodies were detected by fluorescently labelled anti-rat AlexaFluor 488 and anti-rabbit IgG AlexaFluor 647 antibodies (Thermo Fisher Scientific). Nuclei were counterstained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained with the following antibodies: rat CD31-eFluor450 (1/500; eBiosciences™, Thermo-Fisher Scientific, San Diego, CA, USA), rat CD45-eFluor450 (1/500 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed with 0.3% Triton X-100 in PBS for three times and the secondary goat anti-rat-Alexa Fluor 568 antibody (Life Technologies, A11077) was added to the slide for 1 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with fluorescent secondary antibodies (Goat anti-mouse IgG DyLight 800 ThermoFisher #SA5-10176 and Goat anti-rat IgG DyLight 800 ThermoFisher #SA5-10024), and images acquired on a LI-COR Odyssey instrument ...
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bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...
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bioRxiv - Biophysics 2022Quote: ... Second antibodies used in this study were Alexa Fluor 647 (anti-mouse A31571, anti-rabbit A31573, anti-rat A21247; Thermo Fisher Scientific), Atto 647N (anti-mouse 50185-1ML-F ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 647 donkey-rat IgG (1:1,000; Thermo Fisher Scientific, Rockford, IL, USA). Liver ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA, high affinity 3F10, or 1:50,000 Invitrogen α-Histone H3) in 5% non-fat dry milk ...
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bioRxiv - Developmental Biology 2022Quote: ... goat anti-rabbit IgG H&L (Alexa Fluor®) 568 & goat anti-rat IgG H&L (Alexa Fluor®) 568 was from ThermoFisher and was used at a 1:400-fold dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed in PBST and incubated with Alexa Fluor 555 donkey anti-rat IgG secondary antibody (1:500; Thermo Fisher Scientific) for 1 h at 28°C ...
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bioRxiv - Genetics 2022Quote: ... The following secondary antibodies were used at 1:200: Alexa Fluor 555 goat anti-rat (Thermo Fisher, cat# A-21434, RRID: AB_2535855), FITC goat anti-mouse (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2022Quote: ... The leaflets were then incubated for 2 days in the dark at 4°C with goat anti-Rat IgG Cross-adsorbed Alexafluor 594 secondary antibody dilution 1:500 (#A11007, Thermo Fisher Scientific), washed 3 times in 1 mL PBS (pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with secondary antibodies (anti-mouse-Alexa Fluor 488 and anti-rat-Alexa Fluor 647) and NeutrAvidin-DyLight 594 (ThermoFisher Scientific 22842) in blocking buffer (10% goat serum ...
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bioRxiv - Neuroscience 2022Quote: ... rats of either sex and then digested in papain (0.5 mg/mL in Earle’s balanced salt solution) (Thermo Fisher Scientific Inc., MA). Dissociated cells were washed with and triturated in 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies (Donkey anti-goat alexa fluor 594, Donkey anti-rabbit alexa fluor 594, Donkey antirabbit alexa fluor 488, Donkey anti-rat alexa fluor 594; Invitrogen, 1:1000) were incubated with PBT-BSA for 1h at room temperature and 24h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Jurkat cells were incubated with 100 μl Alexa Fluor® 488-labeled anti-rabbit or anti-rat antibody (2 μg/ml, Thermo Scientific) and 293T cells were incubated with 100 μl Alexa Fluor® 647-labeled anti-rabbit antibody (2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... slides were washed with PBS and incubated with goat anti-rat antibody labelled with Alexa Fluor™ 647 (1:500, ThermoFisher Scientific). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... anti-rabbit, and anti-rat antibodies conjugated with Alexafluor dyes (Alexa 546, and Alexa 633) were used as secondary antibodies (ThermoFisher, 1:500). Immunolabeled sections were mounted on glass slides in PBS buffer with 70% glycerol for confocal LSM scanning ...
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bioRxiv - Biochemistry 2024Quote: ... The next day cells were washed 3x with PBS and probed with secondary antibodies: 1:2000 Alexa Fluor® 488 Goat Anti-Rat IgG (Invitrogen, 10729174) or 1:1000 phalloidin (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... transfected cells were seeded at the density of 2000 cells/well in 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco BRL) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor goat anti-rabbit 594, A11037; Alexa Fluor goat anti-rabbit 488, A11008; Alexa Fluor goat anti-rat 594, A11007; Invitrogen, Waltham, MA) before mounting with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Immunology 2024Quote: ... followed by anti-mouse IgG (H+L)-Alexa Fluor 568- or anti-rat IgG (H+L)-Alexa Fluor 647-conjugated secondary antibody (Thermo Fisher Scientific) at 1:500 dilution for 30 min ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed in PBS-Tween (0.5% Tween 20 in 1x PBS) and incubated in goat anti-rat IgG Alexa Fluor® 488 (1:200; A11006; Life Technologies) antibody for 45 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed with PBS twice and incubated with the fluorescent secondary antibody (Alexa Fluor 488 anti-rat, Molecular Probes A-11006) diluted in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rat IgG (H+L) (both from Invitrogen Molecular Probes), for 1 h ...
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bioRxiv - Cell Biology 2023Quote: Rat Basophilic Leukemia (RBL-2H3) cells (ATCC, CRL-2256) were maintained in monolayer cultures with MEM growth medium (Life Technologies, Carlsbad, CA) supplemented 20% heat inactivated Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... for 48 hours at 4°C and then secondary antibodies donkey anti-rat Alexa Fluor 488 and donkey anti-goat Alexa Fluor 555 (1:300, Invitrogen, Waltham, MA) at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... NRVMs were isolated and cultured as previously described.23 The hearts from D0-3 rat pups were isolated and rinsed in HBSS (ThermoFisher Scientific, 14025092) before placed in trypsin-EDTA 0.05% (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes and microglia/macrophage cells were labeled using rat anti-GFAP (1:300; Cat# 13-0300, Thermo Fisher Scientific, Waltham, MA, U.S.A) and rabbit anti-IBA1 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the following secondary antibodies diluted in blocking buffer: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% w/v Triton X-100 and incubated with goat anti-rat Alexa Fluor 488 (1:1000, catalog no. A11029, Thermo Fisher Scientific) secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% w/v Triton X-100 for 5 min and then incubated for 1 hour at room temperature with the following secondary antibodies: goat anti-rat Alexa Fluor 488 (1:1000, catalog no. A11006, Thermo Fisher Scientific), goat anti-chicken Alexa Fluor 488 (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... antibodies for 1.5 h and then with corresponding secondary goat anti-rat Alexa594 and anti-mouse Alexa488 (A-11007 and A-11001, Thermo Fisher Scientific) antibodies (1:300 ...
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A spatially resolved EGFR signaling model predicts the length scale of GAB1-SHP2 complex persistencebioRxiv - Systems Biology 2023Quote: ... EGFR was detected by incubating coverslips for 1 hr at 37°C with anti-rat Alexa Fluor 594 (A-11007, 1:750; Invitrogen, Waltham, MA) and Hoechst nuclear stain (1:2,000) ...
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bioRxiv - Microbiology 2023Quote: ... used in combination with a 1:500 secondary Alexa Fluor 647 donkey anti-rat antibody (Invitrogen Thermo Fisher Scientific, cat. #A-21209).
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed twice into RPMI medium and seeded in a rat tail collagen-1 (5μg/cm2; Gibco, Carlsbad, CA, USA) coated flask for cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Microbiology 2023Quote: ... 1:2,000 rat α-LAMP1 (Developmental Studies Hybridoma Bank) primary antibodies and 1:500 Alexa 488-conjugated goat α-rabbit and Alexa594-conjugated goat α-rat secondary antibodies (ThermoFisher) in blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Alexa Fluor 488 conjugated donkey anti-rat IgG (H+L) Highly Cross-Adsorbed secondary antibody (Thermo Fisher Scientific, Cat. No. A-21208), and Alexa
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bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were washed twice with PBS and incubated with secondary antibodies (goat-anti-rat-A568, cat# A11077, Invitrogen, 1:250) and (goat-anti-rabbit-A568 ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokines were stained at 4°C overnight with FITC or PerCP-e710-conjugated rat anti-mouse IL-1β (NJTEN3; Thermo Fisher Scientific) and PE-conjugated Armenian hamster anti-mouse IL-1α (ALF-161 ...
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bioRxiv - Cell Biology 2024Quote: ... sections were washed three times with PBS and incubated with Alexa 488 (labeled anti-mouse or rat, 1:400) or Alexa 594 (labeled anti-rabbit, 1:400)-conjugated secondary antibody (Invitrogen, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibodies at 1:500 dilution in either 10% NGS in PBS (goat anti-rat, Alexa Fluor 647, Thermo Fisher, A21247) or 1% BSA in PBS (donkey anti-rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with the anti-rat and anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 488 and 546 (1:400, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the embryos were stained with secondary antibodies with Alexa 488 goat anti-rat IgG and Alexa 594 goat-anti-mouse IgG (1:250, Invitrogen, Carlsbad, CA) in blocking buffer overnight ...