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Citations for Thermo Fisher :
4651 - 4700 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Rat Basophilic Leukemia (RBL-2H3) cells (ATCC, CRL-2256) were maintained in monolayer cultures with MEM growth medium (Life Technologies, Carlsbad, CA) supplemented 20% heat inactivated Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed 3 times in PBT and incubated for 4 h at room temperature with anti-mouse or anti-rat secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific), or an secondary alpaca nanobody anti-mouse 488 SMS1AF488-1-100 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were transferred to gelatinized slides and immunofluorescence staining was performed using a human CD45 antibody (Rat. YAML501.4; Thermo Fisher Scientific, USA), GFP Polyclonal Antibody (A-11122 ...
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bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
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bioRxiv - Biochemistry 2024Quote: ... The following secondary antibodies were used at a 1:1000 dilution for cultured cells: donkey anti-rat Alexa Fluor 488 (Invitrogen A-21208) and donkey anti-mouse Alexa Fluor 488 (Invitrogen A-21202) ...
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bioRxiv - Immunology 2024Quote: ... for one hour and incubated overnight with the primary antibodies (rabbit-anti-Iba-1, 1:200; Wako, USA; rat-anti- mCherry, 1:2000, Thermo Fisher Scientific). After washing in PBS ...
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bioRxiv - Immunology 2024Quote: ... rat or rabbit IgG (Invitrogen Clone A10043 Lot 753765; Invitrogen Clone A11055 Lot 2211210; Invitrogen A21447 Lot 872645; Invitrogen A10043 Lot 753765) at 1:500 in PBS+0.3% triton X-100 overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and rat pAbs horseradish peroxidase-conjugated (HRP-Ab) (#111-035-003, #115-035-003, Jackson Immunoresearch Laboratories; #31470, Invitrogen; 1:5000 dilution).
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked with 1% normal goat serum (NGS) in 1X PBS for 1 h followed by incubation with primary antibodies [rat anti-mCherry (Thermo Fisher, #M11217)] overnight at 4°C for standard immunohistochemistry.
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bioRxiv - Immunology 2024Quote: ... Whole- mounts were then washed 3 times for 5 min at room temperature in PBS followed by incubation with Alexa Fluor 568 anti-rat (Invitrogen, 1:500) for 1 hour at room temperature in PBS with 1% BSA and 0.5% Triton-X-100 ...
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bioRxiv - Developmental Biology 2024Quote: ... both yolk sacs and embryos were incubated overnight at 4°C with secondary antibodies to chicken anti-rat Alexa Fluor 488 (Life technologies, A21470) diluted in CAS-Block ...
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bioRxiv - Molecular Biology 2024Quote: ... with 100 nM of either ON-TARGETplus Non-targeting Control siRNA Pool (Dharmacon #D-001810-10-05) or On-TARGETplus Smartpool siRNA targeting rat ACLY protein (Dharmacon #L-089773-02-0005) using lipofectamine RNAimax (Thermofisher Scientific #13778075), as per manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cancer Biology 2021Quote: RNA was transcribed from linearized pGEM plasmids using the MEGAscript T7 Transcription kit (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding S variants were transiently transfected into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Cell Biology 2020Quote: ... prior to being recombined into expression plasmids by LR cloning (Life Technologies, 11791-020). Plasmid constructs were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2020Quote: Transfection of plasmid DNA was performed using Lipofectamine LTX reagent or Lipofectamine 2000 (Invitrogen), and all RNA transfections were performed at a final concentration of 50 nM using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were introduced into cells using Lipofectamine LTX Plus Reagent (Invitrogen Cat#15338-100), according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... HEK cells were transiently transfected with plasmid DNA constructs using Lipofectamine 2000 (Invitrogen/ThermoFisher) in optiMEM media (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK cells were transiently transfected with plasmid DNA constructs using Lipofectamine 2000 (Invitrogen/ThermoFisher) in optiMEM media (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... was used to construct a donor plasmid containing the pCR8/GW/TOPO (Thermo Fisher) plasmid backbone ...
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bioRxiv - Cell Biology 2019Quote: ... Endotoxin-free miniprep or midiprep plasmid DNA transfections were performed using Lipofectamine 2000 (Invitrogen). On the day of transfection ...
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bioRxiv - Immunology 2021Quote: ... by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Supernatants from transfected cells were collected 3 days after transfection ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Genetics 2021Quote: ... Minicircle DNA is then isolated using the Purelink HiPure Plasmid Filter Midiprep kit (Invitrogen). To ensure complete removal of the parental plasmid ...
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bioRxiv - Genetics 2021Quote: ... Cells at 80% confluency were transfected with plasmids using Lipofectamine 2000 (Life Technologies Ltd.) as described previously26.
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bioRxiv - Microbiology 2020Quote: ... Plasmid was ligated using 1 μL (1U/μL) T4 DNA Ligase (Invitrogen, 15224-017) for 5 min at room temperature and subsequently 2μl plasmid was transformed into 100μl chemically competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... they were transfected with the reporter plasmid in the presence of JetPei reagent (Invitrogen). They were incubated for 14 h and then rinsed with fresh medium ...
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bioRxiv - Molecular Biology 2021Quote: ... wild-type or mutant IPMK cDNAs were subcloned into pFASTBAC1 plasmid (Thermo Fisher Scientific) with an N-terminal FLAG epitope ...
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bioRxiv - Developmental Biology 2021Quote: ... E14Tg2a ESCs were transfected with the eSpCas9 plasmids using lipofectamine 3000 (Invitrogen cat. L3000001). After 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection was performed using Lipofectamine™ 2000 (Thermo Fisher Scientific, Waltham, MA, USA) or Viafect (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were transfected with two sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Lot # 2125386), and 24 hours later ...
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bioRxiv - Evolutionary Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: Plasmids were sequenced using either BigDye™ Terminator v3.1 sequencing kit (Applied Biosystems, 4337458) and analyzed in house or samples were sequenced commercially using the GENEWIZ sanger sequencing service.
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bioRxiv - Immunology 2022Quote: ... , we constructed the expression plasmids by synthesizing the DNA fragments from GeneArt (Life Technologies) and cloned them into the phCMV3 vector (Genlantis ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2.5 µg GFP-GLT-1 plasmid using Lipofectamine 3000 (Invitrogen) (1:2 DNA/Lipofectamine ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfection of plasmids or siRNAs was performed using Lipofectamine 3000 or RNAiMAX (Life technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... All plasmids were transfected into U2OS or HaCaT cells using Lipofectamine 3000 (ThermoFisher Scientific) or Xfect Transfection Reagent (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... The target plasmid used in the assays is a pJET1.2 derivative (Thermo Fisher Scientific). The plasmid was added to the reaction mixtures to the final concentration of 2 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... to generate expression plasmids for the Bac-to-Bac baculovirus expression system (Thermo Fisher). Mutations were made using the wild-type EfPiwi expression plasmid as a template and amplifying the protein using oligonucleotide primers containing the desired mutation ...
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bioRxiv - Molecular Biology 2021Quote: ... we mixed 500 ng of plasmid DNA in 50 μl of OptiMEM (ThermoFisher Scientific) and 3 μl of Lipofectamine 2000 in 47 μl of OptiMEM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... STBLII cells were used for maintenance of plasmids containing tandem repeats (Invitrogen cat#10268019). Primer sequences for plasmid constructions are given in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... BAC DNA extraction was performed using Hi-Pure Plasmid DNA Extraction Kit (Invitrogen K210017). Nick translation of BAC DNA was performed using the Nick Translation kit (Roche 11 745 808 910) ...
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bioRxiv - Neuroscience 2019Quote: ... The Cre-NLS (from the pBS185 plasmid from the former Life Technologies, Gaithersburg, MD) and SV40 poly A signal (from the pTRE-tight plasmid from Clontech Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... but plasmid DNA was delivered in serum-free OptiMEM (Gibco, Fisher Scientific, Waltham MA). A ratio of 3:1 (3 µL of HP reagent ...
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bioRxiv - Cell Biology 2019Quote: ... but plasmid DNA was delivered in serum-free OptiMEM (Gibco, Fisher Scientific, Waltham MA). A ratio of 3:1 (3 µL of HP reagent ...
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bioRxiv - Molecular Biology 2019Quote: The plasmids were transfected into HeLa TO cells using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... All PCR products were cloned using the pCR2.1 TOPO™ TA plasmid (Invitrogen, USA). All plasmids were extracted with the E.Z.N.A.™ Pasmid mini kit Omega Bio-Tek ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then transfected with plasmid using the Lipofectamine 3000 reagent (Invitrogen # L3000001) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... SiHa and C33A cells were transfected with pSox2/EGFP plasmid using Lipofectamine 2000 (Invitrogen). Selection was performed using standard culture medium with 1mg/mL G418 ...