Labshake search
Citations for Thermo Fisher :
4301 - 4350 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: ... with plasmids expressing wildtype (WT) or mutant TRPM2 channel and pEGFP-N1 (Invitrogen) that expresses enhanced green fluorescent protein (GFP ...
-
bioRxiv - Genetics 2024Quote: The following plasmids were purchased: pLenti6.2/V5-DEST (Thermo Fisher Scientific Cat. # V36820); pENTR2B (Thermo Fisher Scientific Cat ...
-
bioRxiv - Immunology 2023Quote: ... DNA was extracted using the GeneJET Plasmid Maxiprep Kit (Thermo Fisher Scientific, K0492).
-
bioRxiv - Immunology 2024Quote: ... and cells were transfected with DNA plasmids was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... mixed with the relevant plasmid and subsequent culturing under selection with G418 (Gibco) supplemented EX-CELL 420 serum-free media (Merck).
-
bioRxiv - Immunology 2024Quote: ... The *192C*24 and K183S/K184S/*192C*24 plasmids were produced by ThermoFisher. The pmaxGFP vector was provided by Lonza ...
-
bioRxiv - Cell Biology 2024Quote: ... were transfected with a plasmid solution containing 1 ml of Opti-MEMTM (Gibco), 60 µl of 1mg ml-1 polyethylenimine (PEI ...
-
bioRxiv - Cancer Biology 2024Quote: ... completed to 500 ng total DNA with pcDNA3.1 plasmid (V79020, Thermo Fisher Scientific) using CalFectinTM Cell Transfection Reagent (SL100478 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Plasmids and RLuc-RNA reporters were transfected using the Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... which is based on the pcDNA3.1 plasmid (Thermo Fisher Scientific, Waltham, MA, USA). Clones with wild-type or mutant genomic inserts were selected and verified by sequencing the cloned DNA fragments ...
-
bioRxiv - Immunology 2023Quote: ... and pRL-TK Renilla plasmid using lipofectamine 3000 reagent (Thermo Fisher, USA, No.L3000015). The concentrations of PD1 promoter region and control vector were optimized at same level before the transfection into HEK293T cells ...
-
bioRxiv - Genomics 2022Quote: ... Recombinant plasmids were transfected into cell lines using Lipofectamine 2000 (Invitrogen; cat.no: 11668019). Vector backbone and unmethylated constructs were used as internal controls for comparison ...
-
bioRxiv - Genetics 2023Quote: Purified PCR products or plasmids were sequenced by BigDye Terminator 3.1 (Applied Biosystems). Primers used for sequencing are listed in Table S1 ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmid constructs were transiently co-transfected to COS7 cells using Lipofectamine2000 (Invitrogen) approximately 24 hours before the data acquisition ...
-
bioRxiv - Neuroscience 2023Quote: Base-editor plasmids were codon optimized and synthesized by GeneArt (Thermo Fisher Scientific). The base editor was intein-split to two halves in the SpCas9 sequence at amino acid positions 310 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA or siRNA of phosphatases was transfected using Lipofectamine 2000 (Invitrogen; 52887) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmids (3ug per well) were transfected into HEK293 cells using lipofectamine 2000 (Invitrogen), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The PCR fragment was integrated into the pcDNA5/FRT/TO plasmid (Thermo Fisher) by InFusion Snap assembly (Takara) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µg of each plasmid was mixed with OPTI-MEM (Thermo Fisher Scientific) containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000 ...
-
bioRxiv - Cell Biology 2023Quote: ... and the respective construct plasmid using TurboFect (Thermo Fisher Scientific, Waltham, MA, USA). 48h post transfection ...
-
bioRxiv - Developmental Biology 2023Quote: ... Amplicons were introduced to pDONR221 entry plasmids using BP clonase (11789100, ThermoFisher Scientific), and subsequently to destination plasmids using LR clonase (11791020 ...
-
bioRxiv - Microbiology 2023Quote: ... and cloned in the plasmid pCR2.1 using the TOPO-TA CloningTM Kit (Invitrogen).
-
bioRxiv - Neuroscience 2023Quote: ... Cells were transfected 24 h later with plasmid DNA with Lipofectamine 2000 (Invitrogen). HEK cells were fixed in 4% PFA 24 h after transfection and washed three times with PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Lentivirus was created by transduction of each plasmid with Lipofectamine 3000 (Invitrogen, L3000075) into HEK293T cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... Capped sense RNA was synthesized from linearized plasmids using mMessage mMachine kit (Invitrogen) and RNA was purified using RNeasy Mini kit (Qiagen) ...
-
bioRxiv - Neuroscience 2023Quote: All expression and rescue plasmids were generated via the multisite gateway system (Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... After mixing the plasmids in 200 μL of Opti-MEM (ThermoFisher, Woolwich, UK), Fugene HD (Promega ...
-
bioRxiv - Cell Biology 2023Quote: ... MCF-7 cells were transfected with MitoDendra2 plasmid DNA using Lipofectamine 2000 (Invitrogen). Selection pressure was provided by medium (DMEM-GlutaMax with 10% FBS ...
-
bioRxiv - Biophysics 2023Quote: ... The plasmid (1.0 µg/dish) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... pfnr and parcA were purified (GeneJET plasmid miniprep kit, Thermo Fisher Scientific, Lithuania) and confirmed by visualization of insert release following restriction digestion and Sanger sequencing (Aggrigenome ...
-
bioRxiv - Molecular Biology 2023Quote: Transfection of expression plasmids (Supplemental Table S1) was performed with Lipofectamine 2000 (Invitrogen) and Opti-MEM (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... Cloning vector pKNG101 plasmid was also amplified using Phusion Master Mix (Thermo Scientific). Afterwards ...
-
bioRxiv - Immunology 2023Quote: ... Plasmids were transiently co-transfected in FreeStyle 293-F cells using 293Fectin (ThermoFisher). All cells were tested monthly for mycoplasma ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and terminator part plasmids into a kanamycin-resistant entry vector using Esp3I (Thermofisher) (level 2) ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNA plasmids clones were transfected in mESCs with Lipofectamine LTX (Thermo Fisher Scientific) according to the manufacturer instructions.
-
bioRxiv - Biochemistry 2022Quote: The EN WT plasmid was transformed into BL21 Star (DE3) competent cells (Invitrogen) for large-scale expression ...
-
Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... the GeneJET Plasmid Miniprep Kit and the GeneJET Gel Extraction Kit from ThermoFisher Scientific were used ...
-
bioRxiv - Cell Biology 2023Quote: ... DNAJB1H32Q,D34N-HA in pcDNA5/FRT/TO mammalian expression plasmid (Thermo Fisher Scientific).69 Gene sequences were verified by sanger sequencing.
-
bioRxiv - Developmental Biology 2023Quote: ... gRNAs were synthesized from plasmids using a MEGAshortscript T7 Transcription Kit (Invitrogen AM1354) and purified.
-
bioRxiv - Immunology 2023Quote: ... Spike encoding plasmids were diluted in cold OptiPRO SFM (Life Technologies, 12309-050), mixed with ExpiFectamine CHO Reagent (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... Transfection with plasmid DNA and siRNA was carried out using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations.
-
bioRxiv - Cell Biology 2023Quote: ... Cells co-expressing two plasmids were transiently transfected with Lipofectamine 2000 (Invitrogen #11668019) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... The plasmid expressing the PhiC31 integrase was transfected using Lipofectamine 3000 (Life Technologies), in combination with the Zdhhc20 plasmid containing a PGK promoter ...
-
bioRxiv - Cell Biology 2023Quote: ... and pMD2G plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... or G32R null biosensor plasmids using Lipofecatime 3000 or 293fectin (Thermo Fisher Scientific). Flow cytometry was used to select and enrich for the population of cells expressing respective biosensors ...
-
bioRxiv - Developmental Biology 2023Quote: ... The promoter fragment was successfully cloned into an existing pJet1.2 plasmid backbone (ThermoFisher) containing an mOrange2 (mOr2 ...
-
bioRxiv - Cell Biology 2023Quote: ... Subcloned plasmids were used to transform into Expi293F cells (Thermo Fisher Scientific, # A14527) using the Expi293 expression system (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: Plasmids DNA repair templates were designed using a pMK (Life Technologies, Carlsbad, CA) vector backbone ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK293FT cells were transfected with a plasmid encoding GFP-GPI using Turbofect (Invitrogen) while seeding and according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Ligated plasmid products were introduced by heat shock transformation into competent Stbl3 (Invitrogen). The cloning products were confirmed using Sanger sequencing ...