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Citations for Thermo Fisher :
4401 - 4450 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006], Goat anti-Rabbit 594 [Invitrogen, A11037], Goat anti-Mouse [Invitrogen, A11029]) diluted in blocking solution was added to the tissues and incubated for 3 hours at room temperature ...
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bioRxiv - Genetics 2021Quote: ... for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit IgG Alexa 647 (A21244) goat anti-rat IgG Alexa 488 (A11006) and Alexa 488 conjugated phalloidin (A12379) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by secondary antibody coupled to 10-nm gold (British Biocell or Bbisolutions) and secondary fluorescence antibodies (donkey-anti-rat Alexa594 cat# A-21209, Molecular Probes Europe B.V. ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were incubated with 4% paraformaldehyde in 0.1 M phosphate buffer for 30 min at 37 °C and then incubated with rat anti-PROM1 (Thermo Fisher Scientific) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... 2×104 cells were resuspended in serum-free medium and applied to the top of a membrane coated with collagen I (rat-tail) (Gibco- A1048301). Transmigration assays were carried out using a Corning Transwell Costar apparatus (6.5 mm diameter and 8 µm pore size ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed with PBS and incubated with secondary anti-rabbit and/or anti-mouse or anti-rat IgG conjugated with Alexa-Flour 488 or Alexa-Flour 568 (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and then for 1 h at room temperature in 2 µg/mL Alexa 488-conjugated donkey anti-rat antibody (A-21208, Thermo Fisher). Slides were mounted with ProLong Gold containing DAPI (P36931 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2×103 sorted naked mole-rat cells were added to 3ml of HSC005 supplemented with 1% Penicillin/Streptomycin and 1X GlutaMAX (both Thermo Fisher), grown for 14d at 32°C ...
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bioRxiv - Neuroscience 2021Quote: Nuclear fractions from ipsilateral sciatic nerve injury sites from each rat were isolated with a NE-PER Nuclear and Cytoplasmic Extraction Kit (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... followed by washes in PBS + 0.05% Tween-20 and 30 minutes incubation in goat anti-mouse Alexa fluor 633 and goat anti-rat Alexa fluor 488 (1:25, A21050 and A11006, Molecular Probes). CombiCoverslips were mounted with ProLong Diamond Antifade reagent (P36965 ...
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bioRxiv - Molecular Biology 2021Quote: ... media with 100 nM siRNA (rat siGabarapl1; siGENOME SMARTpool, Dharmacon; Figure S4a) vs non-targeting scrambled siRNA with Lipofectamine RNAiMAX (Life Technologies) for 16 hours ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were incubated for 24 hours in secondary antibodies – goat anti-rat IgG antibodies (Alexa Fluor 488 conjugated; Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were incubated for 24 hours in secondary antibodies – goat anti-rat IgG antibodies (Alexa Fluor 488 conjugated; Molecular Probes, Invitrogen, Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were fixed and stained with anti-rat or anti-mouse secondary antibody conjugated to Alexa 568 (1:200, A11077, Thermo Fisher) or Alexa 647 (1:800 ...
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bioRxiv - Plant Biology 2020Quote: ... The seedlings were incubated and gently shaken in the dark at RT in 500 μL of a 1/10,000 (v/v) dilution of secondary antibody (anti-rat-Alexa Fluor 488; Thermo Fisher Scientific) in PBS for 90 min ...
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bioRxiv - Microbiology 2020Quote: ... and 50% FBS followed by 2 h incubation with primary antibodies and a subsequent incubation with a suitable secondary antibodies in PBS for 45 min (anti-rabbit Alexa Fluor 488 and anti-rat 568; Molecular Probes) and DAPI in PBS for 15 min (Life technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Slides were then washed 3 times in 1% BSA/1xPBS and were incubated in 1:1000 dilution (in 1% BSA/1xPBS) of the following secondary antibodies: Alexa488-conjugated goat anti-rat (Molecular Probes) and Alexa647-conjugated goat anti-rabbit (Molecular Probes ...
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bioRxiv - Pathology 2021Quote: ... tissues were washed in PBS and probed using a goat-anti-rat IgG 488 secondary antibody (1:500, Invitrogen A-11006) for one hour ...
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bioRxiv - Microbiology 2021Quote: ... wells of 96 wells plate were coated with 100 µL of 40 µg.mL-1 rat tail type I collagen in sterile PBS (Life Technologies, NY) at 4°C for 18 hours ...
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bioRxiv - Plant Biology 2021Quote: ... For immunoblotting of A-ZIP53 the blocked blot was incubated with the 1:5000 dilution of HRP labeled rat anti-T7 antibody (Thermo Scientific) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: Deep-sequencing of total RNA isolated from neonatal rat cardiomyocyte cultures was performed 72 hr after transfection of mimics (Life Technologies) of hsa-miR-106b-5p ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution, Molecular probes) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... RAW 264.7 cells were stimulated with the ID8 cell-derived CM supplemented with 5 μg/mL of IL-18 neutralizing antibody (R&D, Minneapolis, MN, USA) or 5 μg/mL of rat IgG1 Isotype as a control (Thermo-Fisher Scientific).
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Either 1x104 whole marrow or 1x103 sorted naked mole-rat cells were added to 3ml of HSC005 supplemented with 1% Penicillin/Streptomycin and 1X GlutaMAX (both Thermo Fisher), equally divided into two 35mm dishes ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in D-PBSTB and incubated for 1hour 30 min at room temperature with secondary antibodies – donkey anti-rat Alexa 555 (dilution 1:1000; Molecular Probes) and donkey anti-rabbit Alexa 647 (dilution 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Collagen matrix was prepared on ice by adding 10x PBS (to make 1x PBS) and 1N NaOH (to make 25mM NaOH) to Collagen I (Rat tail, Thermo Fisher). Organoids were treated with ERW medium ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then washed three times for 15 minutes in 0.1 % Tween-20 in PBS and incubated in PEMBALG containing monoclonal anti-rat antibody conjugated with Alexa488 (1:1000 dilution, Life Technologies) for one hour ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 30 min with goat anti-rabbit Cy5 (Immunoresearch 111 175 144) or goat anti-rat FITC (Invitrogen A11006) secondary antibodies and DAPI (10 μg/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed four times for 5 min with PBS and incubated for 2 h in the dark with Alexa Fluor 568-conjugated donkey anti-rat IgG (Life Technologies) at a dilution of 1:300 ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed with PBS-S-T and incubated for 1h at 37°C with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed three times for 5 min with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies were Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 568 goat anti-rat (red) (used at 1:250 dilution in blocking solution, Life Technologies).
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bioRxiv - Neuroscience 2022Quote: We extracted total RNA from PFC and NAc tissues dissected from 6 rat brains using Trizol Reagent (Invitrogen, Cat, num. 15596018) and Zirconium Beads RNase Free (Next Advance ...
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bioRxiv - Bioengineering 2022Quote: hCMEC/D3 brain endothelial cell line was maintained in tissue culture flasks pre-coated with 0.15 mg/mL rat collagen I in a humidified incubator at 37 ºC and 5% CO2 (Isotemp, Thermo Fisher Scientific). hCMEC/D3 cells were maintained in complete growth medium comprising of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Microbiology 2021Quote: ... Staining was performed for 1 hour with primary antibodies at the following dilutions: rat mouse anti-SAG1 (1:1000, Thermo Scientific), rabbit anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... 20μm sections were incubated with fluorescently conjugated antibodies directed either against mouse IgG, or the IgG of control species (rat, goat or rabbit) (all raised in donkey, Thermo Fisher) in PBS for 2h ...
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bioRxiv - Neuroscience 2021Quote: ... RTN3 was knocked down in DRGN/retinal cultures using an siRNA to rat RTN3 (siRTN3; Assay ID 251374, cat no. AM16708, ThermoFisher Scientific). Protrudin (siProtrudin ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed and incubated for 60 min at room temperature with an appropriate secondary antibody: anti-rat (1:500, Thermo Fisher Scientific Cat# A-11006 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor 594–conjugated anti–rat IgG and Alexa Fluor 488–conjugated anti–mouse IgG)(Molecular Probes).
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bioRxiv - Microbiology 2022Quote: ... PostF APC/DyLight755 and PE/Dylight650 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2019Quote: ... NeoMarkers) and anti-Laminin (4H8-2, MilliporeSigma) antibodies followed by anti-rabbit Alex-488 and anti-rat Alexa-568 (Molecular Probes) were used for detection of FLT1 expression in capillaries ...
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bioRxiv - Biochemistry 2019Quote: ... all used at 1:10,000 dilution in TBS with 0.1% v/v Tween-20 (TBS-T) and horseradish peroxidase-conjugated rat IgG secondary antibody (#31470, Thermo Fisher Scientific) used at 1:10,000 dilution in 5% non-fat dry milk dissolved in TBS-T.
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bioRxiv - Pathology 2020Quote: ... cryoslides were incubated for 2 hours at room temperature with secondary antibodies (AlexaFluor 488-, 555-, 647-, or Cy3-conjugated anti-rabbit, rat, mouse, goat IgG) (1:400, Life Technologies, Waltham ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...