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Citations for Thermo Fisher :
4451 - 4500 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Endothelial cells were then purified by a 10-minute incubation in suspension with magnetic sheep anti-rat Dynabeads (Invitrogen, Carlsbad, CA) coated with rat anti-mouse PECAM-1 (rat ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×104 cells were seeded on top of 1.7mg/mL collagen (type I, rat tail) with or without Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
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bioRxiv - Neuroscience 2021Quote: ... Appropriate secondary fluorescent antibodies were used (Alexa Fluor 488 Goat Anti-rat, #32731; 1:1000; Invitrogen, US; and Alexa Fluor 568 Goat Anti-rabbit, #11011; 1:1000; Invitrogen, US). For Cell nuclei labeling ...
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bioRxiv - Physiology 2021Quote: ... Alex Fluor 546 donkey anti-rabbit secondary antibodies and Alex Fluor 647 goat anti-rat secondary antibodies (1:500; Molecular Probes) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation was performed for 30-60 minutes in room temperature with anti-rat Alexa 555 (1:400, Invitrogen, A21434). Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma ...
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bioRxiv - Immunology 2020Quote: ... PostF APC/DyLight755 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and HEK293T cells derived from human embryonic kidney and transformed with the SV40 large T-antigen were cultured in Dulbecco’s Modified Eagle Medium (Nissui). ARL cells (rat liver epithelial cells; Japan Health Sci. Found., JCRB0248) were cultured in Williams’ Medium E (Gibco BRL). Media were supplemented with 5% fetal calf serum (Gibco BRL) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then washed and bound IgA was detected with a PE-conjugated rat anti-mouse IgA (mA-6E1, 4 μg/ml; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... HiPerFect transfection reagent was used to transfect cells with small interfering (si) RNA oligoribonucleotides targeted to rat ATF6 (assay ID# RSS315363, Stealth siRNA, Thermo Fisher). A non-targeting sequence (cat# 12935300 ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.1M PBS (pH 7.4) followed by 180 ml (rats) or 20 ml (mice) of fresh 4% paraformaldehyde (PFA, pH 7.4) (Fisher Scientific, Hampton NH). Brains were rapidly removed ...
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bioRxiv - Genetics 2019Quote: ... 15 minutes each at 4°C) in PBS-T 0.05% and incubated with either an Alexa Fluor 488 goat anti-rat IgG (Thermo Fisher Scientific) or an Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... they were subsequently stained for 30 minutes with appropriate secondary antibodies 1000× diluted in PBS (Goat anti-Rat AF488 (Invitrogen, A11006), Goat-anti-Rabbit AF488 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The FACS sorted cells were then seeded at a density of 2e4 cells per well in rat tail collagen (Invitrogen, A1048301) coated 96-well CellCarrier plates (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Physiology 2022Quote: ... the sections were further incubated with Alexa647-conjugated donkey antibody to goat IgG (for rats injected with Alexa647-conjugated CTb) or Alexa488-conjugated donkey antibody to goat IgG (10 μg/ml; A11055, Thermo Fisher) (for rats injected with Alexa488-conjugated CTb ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide as described above (for rats injected with Alexa647-conjugated CTb) or with Alexa647-conjugated tyramide (1:50–1:100; B40958, Thermo Fisher) in PBS containing 0.0002– 0.0015% of H2O2 for 5 min (for rats injected with Alexa488-conjugated CTb) ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were mixed with either 0.8 μg/mL of rat anti HA antibody and 30 μL of pre-washed dynabeads protein G (Thermo Fisher; 10003D) or 40ul of pre-washed anti-Flag M2 affinity gel (Millipore Sigma ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of was isolated from cardiac fibroblasts as described above and subjected to GeneChip analysis (GeneChip Arrays platform with Clariom™ S Assay, Rat; Affymetrix). Two independent biological replicates were prepared for cardiac fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Rat pheochromocytoma cell line PC12 (ATCC Cat# CRL-1721; RRID:CVCL_0481, male) was grown in RPMI 1640 media (Gibco Cat#11875-101) containing 10% horse serum (HS ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords isolated from embryonic day 14 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-rat IgG (catalog #62-9520) and Zysorbin-G (catalog #10-1051-1) were from Invitrogen (Carlsbad, CA). The mouse IgG1 (catalog #401407) ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a GC B cell cocktail including: rat anti-mouse CD19 clone 1D3 PerCP-Cy5.5 conjugate (Fisher Scientific, BDB551001), hamster anti-mouse CD95 clone JO2 PE conjugate (Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and 568 conjugated goat anti-rabbit or anti-rat IgG antibodies (1:500 each; Invitrogen; Carlsbad, CA, USA) were used for fluorescent signal detection ...
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bioRxiv - Cancer Biology 2024Quote: ... The following secondary antibodies and dilutions were used: goat anti-rat Alexa Fluor 488 1:1000 (Thermo Fisher Scientific, A-11006), goat anti-rat Alexa Fluor 594 1:1000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... secondary Alexa Fluor 488 antibodies (diluted 1:200 in 0.1% BSA in PBS, goat Anti-Rat-IgM, Thermo Fisher Scientific, Denmark) were added and the sections were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The MS/MS spectra from the LC-MS/MS runs were searched against the Rat UniProt using an in-house Proteome Discoverer (Version PD1.4, Thermo-Fisher Scientific, USA).
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eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... the slices were incubated with secondary antibodies (Goat anti-rat IgG Alexa Fluor 568, A2110844; Goat anti-rabbit IgG Alexa Fluor 568, A2155282; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Microbiology 2024Quote: ... Tissue sections were incubated with rat anti-mouse primary monoclonal mast cell protease-1 (MCPT-1) Ab (1:100, clone RF6.1; Thermo Fisher Scientific), followed by secondary staining with biotinylated rabbit anti-rat IgG (Abcam) ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by the secondary antibody Alexa Fluor 647 goat anti-rat (1:500, Cat# A-21247; Thermo Fisher Scientific, RRID: AB_141778). Later ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006 ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rat IgG (H+L) (both from Invitrogen Molecular Probes), for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... blocked again and stained with secondary antibody (Goat-Anti-Rat conjugated to Cy3 AP136C, SigmaAldrich) and DAPI (D9542, SigmaAldrich) and then mounted with vectashield (1551231, Fisher Scientific,).
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bioRxiv - Neuroscience 2023Quote: ... followed by 2-hour incubation with secondary antibody (1:500 in 1% BSA in PBST; AF488 anti-rat, Invitrogen, A-11006) at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 hours at room temperature in the dark in goat anti-mouse IgG conjugated with Alexa-Fluor 488 or goat anti-rat IgG conjugated with Alexa-Fluor 488 (Molecular Probes, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed for 5 minutes 3 times with PBS followed by incubation with goat anti-Rat IgG (H+L) Alexa Fluor 594 secondary antibody (A11012, Thermo Fisher) at 1:500 dilution for one hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... samples were washed with PBTX for three times and replaced with secondary antibody (1%, goat anti-mouse Alexa Fluor 488 and goat anti-rat Alexa Fluor 568, ThermoFisher UK) and 1 ug/ml DAPI solution ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were washed three times with TBS-Tween 20 followed by incubation with 1:1000 secondary antibody in 2 % milk in TBS-Tween 20 for 1.5 hours (Anti-rat IgG for JIM5 and JIM7 and Anti-mouse IgG for 2F4 couples to Alexa-Fluor; Molecular Probes). The sections were washed three times with TBS-Tween 20 and then incubated with propidium iodide (20 mg ml−1 ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 45 min at R.T ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 1 h at R.T.
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 568 and anti-mouse Alexa Fluor 488 (1/25, A11077 and A11001, ThermoFisher Scientific) for 45 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... including anti-rabbit or anti-mouse Alexa Fluor 568 and anti-rat Alexa Fluor 488 (all 1:1000, Thermo Fisher Scientific), were added and incubated for 2 h at room temperature ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The total dissociated single cells were resuspended in 500 μL sorting buffer (25 mM HEPES, 5 mM EDTA, 1% BSA, in HBSS) containing Normal Rat Serum (1:100; Invitrogen, 10710C) and TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher] ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 488-conjugated anti-mouse IgG (also cross-adsorbed to rabbit IgG and rat IgG) (A-11029; Thermo Fisher Scientific) and Alexa Fluor 568-conjugated anti-rabbit IgG (also cross-adsorbed to mouse IgG ...