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Citations for Thermo Fisher :
4751 - 4800 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 1,5 μg plasmid DNA was transfected using the Lipofectamine 2000 transfection reagent (Life Technologies) according to manufacturer’s protocol ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... and subcloned into the pCR2.1 plasmid using the TOPO-TA cloning Kit (Thermo Fisher). Plasmids were prepared using the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry-UBQLN2 (mCh-UBQLN2) plasmids were diluted in Opti-MEM Reduced Serum Medium (ThermoFisher) and allowed 20 minutes to form complexes with Trans-IT transfection reagent (Mirus) ...
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bioRxiv - Cell Biology 2020Quote: ... Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668, Invitrogen). Histidine-tagged ubiquitin (pCI-His-hUbi ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection of plasmid DNA was performed with Lipofectamine 3000 and P3000 reagent (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The gene encoding for ADR1a was inserted into the pRSET plasmid (Thermo Fisher Scientific), between the NdeI and XhoI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were delivered to the cells by using Plus Reagent and Lipofectamine LTX (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were linearized and in vitro transcribed using a mMessage mMachine T3 (Ambion #AM1348) and T7 kits (Ambion #AM1344) ...
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bioRxiv - Cell Biology 2021Quote: ... A vector backbone fragment was obtained by PCR amplification from plasmid pcDNA3.1/zeo(+) (ThermoFisher). Plasmids expressing DSB repair genes were constructed via Gibson assembly of gene fragment(s ...
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bioRxiv - Genomics 2020Quote: Two µg of plasmid was cut with the FastDigest PstI restriction enzyme (Thermo Fisher) and the 900 bp piece was gel purified using a gel DNA extraction kit (Zymo) ...
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bioRxiv - Cell Biology 2021Quote: ... The cell-plasmid mixture was electroplated using the Neon Electroporation System (Invitrogen, USA. #MPK1025) as per manufacturer’s protocol under the following standardized parameters (1100 volts ...
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bioRxiv - Bioengineering 2022Quote: ... and pLP2 plasmids into HEK293T cells at 80–90% confluency using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... and pLenti-EF1α-hChR2(H134R)-EYFP-WPRE (transfer plasmid) in Opti-MEM (ThermoFisher Scientific) using Lipofectamine 2000 Transfection Reagent () ...
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bioRxiv - Cell Biology 2022Quote: ... and ligated into double digested plasmids with the Rapid DNA Ligation Kit from ThermoFisher Scientific (K1422) ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were transfected with Spike protein expressing pLP plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol and stained for their spike protein expression 72 hours after the transfection as described in Staining and Flow cytometry Analysis ...
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bioRxiv - Molecular Biology 2022Quote: Transfection of plasmid DNA and siRNA was carried out with Lipofectamine 2000 (Thermo Fisher) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... The plasmids (4.0 μg) were mixed with 250 μl of Opti-MEM (Thermo Fisher) and 1.0 μg/μl of PEI Max (8.0 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... AIO-GFP-containing sgRNA plasmids were transfected using Lipofectamine 2000 (Life Technologies/Thermo Fisher) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... AIO-GFP-containing sgRNA plasmids were transfected using Lipofectamine 2000 (Life Technologies/Thermo Fisher) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... All plasmids encoding the candidate OT sensors were cloned into the pDisplay vector (Invitrogen) with an upstream IgK leader sequence and a downstream IRES-mCherry-CAAX cassette for labeling the plasma membrane ...
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bioRxiv - Genetics 2022Quote: ... the Cas9-expressing plasmid (800 ng) was transfected into cells using Lipofectamine 2000 (Invitrogen). Three days after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA5-FRT-TO MTS-BG4 was co-transfected with the pOG44 plasmid (ThermoFisher Scientific), containing the Flp recombinase ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... The mYBX1 plasmid and negative control were transfected into BMCSs using Lipofectamine 2000 (Invitrogen) for 6 hours before proceeding to perform further experiments.
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids and siRNAs were transfected into cells using Lipofectamine 2000 reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with the plasmid pPH-Akt-GFP using Lipofectamine 2000 (Thermo Fisher). After allowing the cells to express the proteins overnight ...
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bioRxiv - Bioengineering 2022Quote: Expi293F cells were transfected with the purified plasmids using ExpiFectamine 293 transfection kit (Invitrogen), where the fusion proteins were expressed and harvested at 96 hrs post-transfection under 8% CO2 at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 0.4ug of plasmid was added to 21 µl Opti-MEM reduced serum medium (Gibco) and 1.2 µl Fugene 6 (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... fibroblasts were transfected with a GFP-VSV-G ts045 plasmid using Lipofectamine 3000 (Invitrogen) as per manufacturer’s instructions and grown at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... from pCS-TP plasmid (Kawakami et al., 2004) cut with Bsp120I (Thermo Scientific, ER0131). Transgenics were imaged at st20 (~84 hpf ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid constructs were transformed into BL21 Star™ (DE3) cells (Thermo Fisher Scientific). The cultures were grown in Terrific Broth media supplemented with ampicillin (Quality Biological ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected to the cells using lipofectamine 3000 transfection reagent (Thermo Fisher). The cells were harvested and analyzed at the time point(s ...
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bioRxiv - Neuroscience 2021Quote: ... 2μg to 6μg of plasmid DNA was diluted in 300ul of Optimem media (Invitrogen) and mixed with the transfection reagent (Fugene or PEI ...
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bioRxiv - Molecular Biology 2020Quote: ... Oligonucleotides were transfected 4 h after the plasmid using Lipofectamine™ 2000 (ThermoFisher, #11668027) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and pVSV-G expression plasmid using PEI transfection reagent (Alfa Aesar/Thermo Fisher Scientific). The virus was harvested at ~48 hours after transfection and stored at −80 °C.
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bioRxiv - Neuroscience 2020Quote: ... Transfection of plasmids in HEK 293T cells was carried out with DNAfectin™ (Invitrogen) following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: All expression plasmids in this study were constructed by Three-Fragment Multisite gateway (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... cells were transfected with 1.25 μg of each plasmid using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was then removed using the TURBOTM DNA-free kit (AM1907, ThermoFisher Scientific), and RNA was recovered by lithium chloride precipitation ...
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bioRxiv - Biophysics 2020Quote: ... The plasmids carrying the gene of each mutant were then transformed into TOP10 (Invitrogen) E ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral plasmids were transduced into HET293T cells along with ViraPower Lentiviral Packaging Mix (Invitrogen) using the Lipofectamine 3000 Transfection Reagent (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... and 1275 ng lentiviral target plasmid using Lipofectamine 2000 and PLUS reagent (Invitrogen, 11514015). Media was refreshed four hours after transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR fragments were cloned into plasmids with either the USERTM enzyme (ThermoFisher Scientific Co.), T4 polynucleotide kinase or T4 DNA ligase from New England BioLabs (Ipswich ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid vectors were transformed in BL21(DE3) competent cells (Invitrogen or Sigma Aldrich) and grown overnight in a starter culture of 50 mL LB media (Fisher Bioreagents ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2.5 µg of Apo plasmid using Lipofectamine 2000 (Thermo Fisher). The media was replaced with 2 ml Opti-MEM (Thermo Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... Genes were then recombined into pEarleyGate 104 and 202 plasmids by LR reaction (Invitrogen) as described previously (Earley et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... and EGFP-PTC encoding plasmid (0.5 ug) using the Neon™ transfection system (Invitrogen) with the following parameters ...
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bioRxiv - Immunology 2019Quote: All plasmid transfections were performed with a commercial transfection kit (Thermo Fisher Scientific, USA). Cells were seeded on 12 mm coverslips in 24 wells for imaging or in 6-well plates for qRT-PCR or immunoblotting analyses ...
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bioRxiv - Plant Biology 2019Quote: ... along with the PRH1 coding sequence into a pENTRTM/SD/D-TOPOTM plasmid (ThermoFisher). The construct was subsequently recombined with several Gateway destination vectors ...
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bioRxiv - Synthetic Biology 2019Quote: ... All cells were transfected with desired DNA plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...