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Citations for Thermo Fisher :
4301 - 4350 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Total protein from B16F10 melanoma was harvested using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) with the Complete mini EDTA-free (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 5 min with 0.05 M PB and mounted on Superfrost Plus (ThermoFisher Catalog# 12-550-15) microscope slides ...
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bioRxiv - Neuroscience 2023Quote: 45 □m Coronal slices of the raphe nuclei and hippocampus were sectioned using a cryostat (Thermo Fisher Scientific) and collected in 1x PBS ...
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bioRxiv - Genetics 2023Quote: ... cells were washed twice with cold PBS and lysed in M-Per Mammalian Protein Extraction Reagent (Thermo Scientific) supplemented with protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... A TR-FAME column (length: 30 m, inter diameter: 0.25 mm, film size: 0.25 μm, 260M142P, Thermofisher Scientific) was used with helium as carrier gas ...
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bioRxiv - Molecular Biology 2023Quote: ... one volume of chromatin was mixed with 1/10 volume of Dynabeads M-270 Epoxy (ThermoFisher, LOT 01063817) coupled to immunoglobulin G (IgG ...
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bioRxiv - Immunology 2023Quote: ... and placed into M Tubes (Miltenyi #130-093-236) with 6ml of RIPA Lysis buffer (Thermo Scientific #89900) with 1x HALT protease inhibitor cocktail (Thermo Scientific #78429) ...
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bioRxiv - Neuroscience 2023Quote: ... Immediately after thoroughly rinsing the muscle with 0.1 M phosphate-buffed saline (pH=7.4, PBS, ThermoFisher Scientific, USA), the interscutularis muscle was immersed with a solution containing 2% glutaraldehyde (Glut. ...
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bioRxiv - Genomics 2022Quote: ... and biotinylated RNA was enriched with streptavidin-coated beads (DYNAL™ Dynabeads™ M-280 Streptavidin, ThermoFisher 11205D). qRT-PCR evaluation was performed also with the primers suggested by Fuchs et al ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µL of dissolving solution (0.1 g/ml SDS in 0.01 M HCl; Thermo Fisher, Rockfold, IL, USA) was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 μL Tris pH 6.5 1 M and 1 μL Proteinase K (20 mg/ mL, Thermo Fisher Scientific) and incubated shaking for 3 hours at 45°C ...
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bioRxiv - Immunology 2023Quote: ... cells of the cultured YUMMER1.7 cell line(31) (a gift from M. Bosenberg) were detached with 0.5% trypsin-EDTA (Gibco) for 3 minutes ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 0.1 M CaCl2 and 0.4 μl of 100 U/μl MNase (88216, Thermo Fisher Scientific) were then added ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription reaction was performed on the same amount of total RNA using M-MLV reverse transcriptase (Invitrogen). Real-time qPCR was performed on the CFX96 Real-Time PCR Detection System using the SsoFast EvaGreen Supermix (Bio-Rad) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged by incubation in 0.5 mM EDTA-PBS (stock: 0.5 M EDTA, AM9260G, Thermo Fisher Scientific). hESCs were seeded at a density of 104 cells/cm2 in Essential 8™ medium ...
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bioRxiv - Developmental Biology 2023Quote: ... in splitless mode equipped with a TG-5MS capillary column (30 m × 0.25 mm × 0.25 μm, Thermo Scientific), using helium as carrier gas (1.0 ml/min) ...
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bioRxiv - Cell Biology 2023Quote: ... Bond-breaker TCEP solution (0.5 M) and protease inhibitor cocktail (100×, EDTA-free) were purchased from Thermo Fisher Scientific (Rockford ...
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bioRxiv - Microbiology 2023Quote: ... isolation window: 1.0 m/z) to acquire MS and MS/MS spectra by Xcalibur 4.0 (Thermo Fisher Scientific). MS/MS spectra of biogenic and synthetic molecules were acquired and directly compared ...
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bioRxiv - Biochemistry 2023Quote: ... for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific, Waltham, MA) in water ...
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bioRxiv - Molecular Biology 2023Quote: ... the PCR product was coupled to 25 μl streptavidin-coated paramagnetic beads (Dynabeads Streptavidin, M-280, Thermo Scientific) for 15 min and washed with 3 × 1000μl of PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then reduced and alkylated with 1 μL of 0.5 M tris-2(-carboxyethyl)- phosphine (ThermoFisher) and 5 μL of 0.4 M chloroacetamide in a 90 °C water bath for 10 min followed with 5 min bath sonication ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 10 min in 0.1 M PBS containing 0.02% DAPI (catalog # D1306, Molecular Probes, Invitrogen). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were subsequently incubated with 20-40 μl Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher, Cat# 11201D) for 2-4 hours at 4℃ ...
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bioRxiv - Cancer Biology 2023Quote: Spectral features (retention time, m/z) pairs were extracted from raw data using Compound Discoverer v3.3 (Thermo Scientific). This step included chromatographic peak alignment ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was measured spectrophotometrically (ε280 21430 M-1cm-1, MW 24.79 kDa) using NanoDrop One (Thermo Scientific). Dialysed MSP1D1 was concentrated to 5 mg/ml using a 10 kDa MWCO Amicon Ultra centrifugal concentrator (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... Annie Newell-Fugate at Texas A&M University) were cultured in Eagle’s minimal essential medium (EMEM; Fisher Scientific) using the same supplements and environments described for HFF culture ...
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bioRxiv - Immunology 2023Quote: ... and 40 µL per mL of complex of 1 M formaldehyde prepared from 37 % stock (Thermo Fisher, 10630813). The complex was incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 10 min in 0.1 M PBS containing 0.02% DAPI (catalog # D1306, Molecular Probes, Invitrogen). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 M imidazole and 30 µl of Ni-NTA agarose beads (Thermo Scientific, MA, USA) were added to the mixture and incubated for 30 min at 4-8°C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein from S and P fractions were mixed with 2 µL 0.5 M DTT and 4x LDS (Invitrogen), incubated at 70°C for 10 minutes and run on NuPAGE 10% Bis-Tris gels in MES/SDS-PAGE running buffer (1M MES ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were collected and cells were lysed with 25µL/well M-PERR (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1:100 protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... Upon immobilization of the samples on streptavidin-coated magnetic beads (Dynabeads™ M-280 Streptavidin, ThermoFisher Scientific, #11205D), oligonucleotides were incubated with 100 or 10 ng FLAG-tagged iMab (Absolute antibody ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was purified via ethanol precipitation (final concentrations of 0.3 M sodium acetate pH 5.2, 0.3 µg/mL glycoblue (Invitrogen #AM9516), and 70% ethanol ...
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bioRxiv - Genomics 2024Quote: ... cells were washed once in antibody incubation buffer (47.5 mL H2O RNAse free, 1 mL 1 M HEPES pH 7.5 (Invitrogen), 1.5 mL 5 M NaCl ...
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bioRxiv - Genomics 2024Quote: ... 100 nL of Basic T-ChIC buffer (47.5 mL Nuclease-free water, 1 mL 1 M HEPES pH 7.5 (Invitrogen), 1.5 mL 5 M NaCl ...
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bioRxiv - Genomics 2024Quote: ... cells were pelleted at 500 g for 4 min and washed once in T-ChIC wash buffer (47.5 mL H2O RNAse free, 1 mL 1 M HEPES pH 7.5 (Invitrogen), 1.5 mL 5 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Plastic petri dishes (TPP, Switzerland) were incubated in 100 μg/mL rat tail collagen I (Gibco A10483-01) in 0.1% acetic acid at 4°C overnight on a 60 rpm shaker ...
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bioRxiv - Cell Biology 2020Quote: ... Donkey Anti-rat IgG (H+L) Alexa Fluor 488 (Thermo Fisher; Cat# A-21208; used at 1:250), and Goat anti-mouse IgM Antibody Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... eBioscience) and an Alexa Fluor 647-goat anti-rat IgG secondary antibody (1:400, A-21247, Life Technologies). Sections were also counter-stained with DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... AlexaFluor®-conjugated secondary antibodies (488, 555, 568, 647-conjugated anti-mouse, rabbit and rat antibodies, ThermoFisher Scientific) were used in immunofluorescence and flow cytometry ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-rat and anti-rabbit secondary antibodies and Alexa Fluor conjugated phalloidin were obtained from Invitrogen (Carlsbad, CA).
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bioRxiv - Microbiology 2021Quote: ... was used as primary antibody (1:500) and goat-anti-rat polyclonal Alexa Fluor 594 (Thermofisher, Waltham, MA) as secondary along with Vicia villosa lectin (Vector Labs Burlingame ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies (goat anti-chicken coupled to Alexa 568; goat anti-rat coupled to Alexa 594 Invitrogen A11007), diluted 1:1000 in washing buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... α3 rat cDNA was ligated into a previously modified Gateway entry vector pENTR1a (Thermo Fisher Scientific, Waltham, MA) between the human ubiquitin C promoter (UbiC ...
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bioRxiv - Biochemistry 2022Quote: ... and probed for 2 h with an FITC-conjugated goat anti-rat antibody (1:300, Thermo Fisher Scientific). VE-cadherin in the superficial vascular plexus and deep capillary plexus was visualized in the retinas by confocal microscopy as illustrated in Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using TaqMan Gene Expression Assay for rat β-actin (Actb, Rn00667869_m1, Applied Biosystems), rat Taste receptor type 1 member 3 (Tas1r3 ...
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bioRxiv - Neuroscience 2021Quote: Slices obtained from VGAT-Venus rats were bulk loaded with 50 μM Fura-2,AM (Invitrogen; Carlsbad, CA) 0.2% Pluronic F-127 in our standard extracellular solution but containing 2 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Slides were then incubated with a combination of the following secondary antibodies: donkey anti-rat 488 (Invitrogen, #A21208), donkey anti-rabbit 555 (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... followed by addition of 100× diluted secondary antibody: FITC-linked anti-rat IgG (Thermo Fisher Scientific, Rockford, IL) for an additional 1 h ...